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Citations for Lucigen :
2251 - 2300 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... PAP was purchased from Lucigen. Snake venom phosphodiesterase (SVPDE ...
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bioRxiv - Molecular Biology 2020Quote: ... Clones were identified ∼2 weeks after sorting and screened by genomic DNA extraction with Quickextract (Lucigen cat.#QE09050) and parental allele-specific PCR probes and GoTaq Green Master Mix (Promega cat.#M712) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids were grown in E.Cloni 10G Chemically Competent Cells (Lucigen, 60107-1) and were verified by Sanger sequencing (Eton biosciences) ...
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bioRxiv - Genetics 2020Quote: ... and used as a template for in-vitro transcription of gRNA by AmpliScribe T7 High Yield Transcription Kit (Epicentre Technologies).
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were then used as templates for in vitro transcription per AmpliScribe T7 High Yield Transcription Kit (Epicentre Technologies) specifications to obtain dsRNAs.
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bioRxiv - Genetics 2020Quote: ... Worms were lysed in DNA Quick Lysate (Epicentre Technologies) for 1 hour at 60°C and the lysate was then used as a template for PCR with Q5 Hot Start High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Genetics 2020Quote: ... and subsequently circularised with CircLigase II (Epicentre). PCR amplification was obtained after 18-21 cycles ...
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bioRxiv - Genetics 2020Quote: ... 2 µl per tube was transformed into two tubes of 50 µl of electrocompetent cells (Lucigen DUO) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was prepared using MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... for qRT-PCR and northern blotting and MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) for RNA-seq ...
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bioRxiv - Genetics 2020Quote: ... for qRT-PCR and northern blotting and MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) for RNA-seq ...
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bioRxiv - Genetics 2020Quote: ... using Masterpure complete DNA and RNA purification kit (Lucigen), genomic DNA for whole genome sequencing was isolated ...
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bioRxiv - Genetics 2020Quote: DNA was isolated using QuickExtract DNA extract solution (Lucigen) for PCR grade DNA or the QIAamp DNA mini kit (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... depleted of genomic DNA contamination using Baseline-Zero DNase (Epicentre, #DB0715K), and depleted of rRNA using a Yeast Ribo-Zero Gold rRNA removal kit (Illumina ...
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bioRxiv - Genetics 2020Quote: ... Total RNA was prepared using MasterPure™ Yeast RNA Purification Kit (Epicentre, Lucigen) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... coli (Lucigen). A small aliquot of the transformant culture was plated to monitor library complexity ...
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bioRxiv - Genetics 2020Quote: ... PCR was performed using EconoTaq and associated PCR reagents (Lucigen) with 4 μL of crude DNA lysate created as described previously 24 from ear punch biopsy specimens of 8 to 14-day-old mice ...
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bioRxiv - Genetics 2020Quote: ... genomic DNA was isolated from sorted and non-sorted cells using the QuickExtract reagent (Lucigen QE09050). In addition ...
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bioRxiv - Genetics 2020Quote: ... DNA was isolated from tail snips of MyD88 CRISPy TAKO and Mock-treated control offspring using Quick Extract (Lucigen, #QE09050). Primers for MyD88 genotyping are listed in Table 1 ...
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bioRxiv - Genetics 2020Quote: ... using their associated buffers or alternatively FailSafe PCR PreMix buffers (Epicentre). Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Bioengineering 2020Quote: ... coli C43(DE3) background (Lucigen) to construct all the strains.
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bioRxiv - Cell Biology 2020Quote: ... and EconoTaq Plus Green 2X Master Mix (Lucigen). Primers used are listed in Table 1.
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bioRxiv - Molecular Biology 2020Quote: ... The pure transcripts were capped using the ScriptCap™ m7G Capping System (Epicentre). Radiolabelled transcripts were obtained by substituting the GTP from the kit with [α32P]GTP.
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Lucigen) harboring the pTA-MOB conjugation helper plasmid (Strand et al. ...
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bioRxiv - Systems Biology 2020Quote: RFP positive A549 VIM-RFP cells were harvested and DNA was extracted using QuickExtract (Epicentre, QE09050). Primers were designed for left homology arm and right homology arm junction PCR (left junction Fwd ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... recovered and used for the 20 µL circularization reaction (100 U CircLigase™ ssDNA Ligase (Lucigen, CL4111K), 50 mM ATP ...
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bioRxiv - Molecular Biology 2020Quote: ... the (+)SHAPE and (−)SHAPE RNA samples were treated with Terminator™ 5′-Phosphate-Dependent Exonuclease (TER51020, EPICENTRE co.), which processively digests RNA with 5′-monophosphate ends ...
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bioRxiv - Molecular Biology 2020Quote: ... The ligation reaction was then treated with PlasmidSafe (Epicentre, Cat#: E3101K) to digest any residual linearized DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA was then treated for 15min at 37°C with 10U RNase R from Epicentre in a 20 μl reaction volume ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from cells using the MasterPure RNA Purification Kit from Epicentre following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... jirovecii-infected patients was extracted using the MasterPure Yeast DNA purification kit (Epicentre Biotechnologies, Madison, WI, USA). Total RNAs for P ...
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bioRxiv - Cell Biology 2020Quote: ... four units of exonuclease (Plasmid-Safe ATP-dependent DNase, Epicentre) was added to the reaction at 37°C (reaction volume 40 µL ...
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bioRxiv - Bioengineering 2020Quote: ... and had genetic material isolated via the MasterPure™ genomic DNA purification kit (Lucigen, Cat. No. MC85200). Genomic DNA was fragmented to 300 bp via Covaris sonication ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... sgRNA was synthesized in vitro as per the suggested protocol using Ampliscribe T7 Flash Transcription kit (Lucigen, USA). 6xHis-Cas9 protein was synthesized as described previously25 ...
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bioRxiv - Neuroscience 2020Quote: ... RNA samples with RNA integrity numbers (RIN) greater than seven (mean RIN 8.5) were processed with Ribo-Zero Gold kit (Epicentre, WI) to remove ribosomal RNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... repaired with End-IT DNA repair kit (Epicentre), A-tailed with Klenow enzyme (New England Biolabs) ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR was carried out using EconoTaq PLUS GREEN 2x master mixes (Lucigen) and primers for target genes and control housekeeping genes ...
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bioRxiv - Genomics 2020Quote: ... first strand synthesis was performed by incubating the pucks in 200 μL of reverse transcription solution (Maxima 1x RT Buffer, 1 mM dNTPs, 2 U/μL Lucigen NxGen RNAse inhibitor ...
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bioRxiv - Genomics 2020Quote: Bead arrays with tissue sections were immediately immersed in 200 μL of hybridization buffer (6x SSC with 2 U/μL Lucigen NxGen RNAse inhibitor) for 30 minutes at room temperature to allow for binding of the mRNA to the oligos on the beads ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre, RJ411250). and 1 μL 20 U/μL SUPERase Inhibitor ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre, RJ411250). and 1 μL 20 U/μL SUPERase Inhibitor ...
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bioRxiv - Biophysics 2020Quote: ... pSMART_ptsG-10aa-sfGFP (“10aa” refers to the first 10 codons) was generated from pZEMB8 (19) using site directed mutagenesis and the pSMART LCKan Blunt Cloning Kit (Lucigen, 40821-2). Briefly ...
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bioRxiv - Biophysics 2020Quote: ... Cells were lysed using Ready-Lyse Lysozyme (Epicentre, R1802M) followed by 0.5% SDS treatment ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were thoroughly resuspended in 50 µl QuickExtract (Lucigen) and incubated at 65 °C for 20 min and 98 °C for 5 min ...
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bioRxiv - Genomics 2020Quote: ... a custom transposome was constructed using EZ-Tn5 transposase (EpiCentre) and a transposon carrying a kanamycin (Km ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted with Epicentre Masterpure Kit (Epicentre Catalog number: MC85200). Sample and sequencing library preparation was done by using the Nextera DNA library preparation kit (Illumina ...
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bioRxiv - Genetics 2020Quote: For single cell clones or bulk sequencing genomic DNA (gDNA) was extracted by quick extract (Lucigen). PCR amplification was performed with LongAmp Polymerase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... rRNA was removed using a Ribo-Zero (Bacteria) kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... or Epicenter MasterPure Complete DNA and RNA Purification Kit (Lucigen, Madison, WI, USA) according to manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... The CCF stool pellets were homogenized with 500 μL Tissue and cell lysis buffer (Lucigen©) by pipetting up and down ...