Labshake search
Citations for Lucigen :
2101 - 2150 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: Random knockout mutants were produced using the EZ::Tn5Tm
Tnp TransposomeTm kit (Epicentre) following the manufacturers recommendations ... -
bioRxiv - Biophysics 2020Quote: ... genomic DNA was isolated using QuickExtract (Lucigen), the sgRNA-targeted sites were PCR amplified and then NGS-sequenced via Genewiz’s EZ-Amplicon service ...
-
bioRxiv - Biophysics 2020Quote: The identified sybody genes of positive clones were chemically-transformed into Escherichia coli MC1061 F- (Lucigen) cells ...
-
bioRxiv - Molecular Biology 2020Quote: ... We then depleted rRNA using the Ribozero Gold kit from Epicentre (RZG1224). cDNA was synthesized from the purified RNA using the Superscript Vilo cDNA sequencing kit from ThermoFisher (11754050 ...
-
bioRxiv - Molecular Biology 2020Quote: C41(DE3) cells (Lucigen) were transformed with the expression plasmid using standard techniques ...
-
bioRxiv - Molecular Biology 2020Quote: ... the genomic DNA of each clone was extracted by using QuickExtract solution (Epicentre) and PCR was carried out ...
-
bioRxiv - Neuroscience 2020Quote: ... gDNA was extracted using DNA QuickExtract Solution (Lucigen) followed by PCR and Sequencing to determine efficient rescue.
-
bioRxiv - Neuroscience 2020Quote: ... and circularized with CircLigase II (Epicentre CL9025K). RPF libraries were PCR amplified with Phusion High-Fidelity DNA Polymerase (NEB M0530A ...
-
bioRxiv - Microbiology 2020Quote: ... and the rRNA was removed with Ribo-Zero Magnetic Gold Kit (Epicentre, USA). The extracted RNA was reverse transcribed with anchored random primers ...
-
bioRxiv - Microbiology 2020Quote: ... transposomes were prepared using an EZ-Tn5 transposase (Epicentre Biotechnology) and a custom transposon carrying a kanamycin resistance cassette amplified from the pUT_Km plasmid (32 ...
-
bioRxiv - Microbiology 2020Quote: ... Next 5 μL of circularization reaction buffer was added (3.3 μL water, 1.5 μL 10x Circligase-II buffer, and 0.5 μL of Circligase-II (Epicentre)) ...
-
bioRxiv - Microbiology 2020Quote: Genomic DNA was isolated using MasterPure complete DNA and RNA purification kit (Epicentre, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... 7.5 μg RNA (harvested using the MasterPure Yeast RNA Purification Kit (Epicentre)) was DNAse treated with the Turbo DNA-free Kit (Invitrogen) ...
-
bioRxiv - Microbiology 2020Quote: ... RNA was harvested from snap-frozen pellets (using liquid nitrogen) using the MasterPure Yeast RNA Purification Kit (Epicentre) and stored at −80 °C ...
-
bioRxiv - Microbiology 2020Quote: ... Germany). MasterPure DNA purification kit (Cat. No. MCD85201) was obtained from Epicentre (Middleton, WI, USA). GoTaq Green Master Mix (Cat ...
-
bioRxiv - Microbiology 2020Quote: ... was isolated using the MasterPure DNA purification kit (MCD85201 Epicentre, Middleton, WI, USA). For constructing nanB-deletion mutants ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1 U/ml Rnase Inhibitor (Lucigen), 2.5 μM Template Switch Oligo ...
-
bioRxiv - Microbiology 2020Quote: ... Purified PCR products were ligated into the pETite vector containing an N-terminal His6 tag (Lucigen) and transformed into HI-Control 10G cells ...
-
bioRxiv - Microbiology 2020Quote: ... NEB Builder reaction mixtures were transformed into E.coli EPI300 cells (Lucigen) and successful integration of the S protein cDNA was confirmed by Sanger sequencing ...
-
bioRxiv - Microbiology 2020Quote: A genomic fosmid library of strain HO-1 was constructed using the CopyControl™ HTP Fosmid Library Production Kit (Epicentre) according to the instructions of the manufacturer ...
-
bioRxiv - Microbiology 2020Quote: ... Positive clones that were capable of producing hydroxylamine and nitrite from ammonium were screened and sequenced using specific pCC2FOS sequencing primers (Epicentre) at SinoGenoMax (Beijing ...
-
bioRxiv - Immunology 2020Quote: ... genomic DNA was extracted from at least 104 harvested cells using the QuickExtract protocol (Lucigen). The target locus was then amplified by PCR with forward and reverse primers where at least one primer annealed outside the integration site (Supplementary Table 1) ...
-
bioRxiv - Neuroscience 2020Quote: ... and 0.5 U/μL Ampligase (Epicentre) in 1× Ampligase buffer] for 30 mins at 37 °C and 45 mins at 45 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... and 0.5 U/μL Ampligase (Epicentre) in 1× Ampligase buffer] without any wash in between ...
-
bioRxiv - Neuroscience 2020Quote: ... and 0.5 μL Tn5 (Lucigen). The mixture was heated to 55 °C for 12 minutes ...
-
bioRxiv - Molecular Biology 2020Quote: ... A library of ∼500,000 independent mini-Tn5 transposon mutants was created by transforming BW25113 ΔascA with an EZ-Tn5
Tnp transposome kit (Epicentre) by electroporation ... -
bioRxiv - Molecular Biology 2020Quote: ... The ligated DNA was packaged into virions using the MaxPlax λ packaging extract (Lucigen #MP5120, Middleton, WI, USA), and the virions were sequenced to determine input barcode frequency.
-
bioRxiv - Molecular Biology 2020Quote: ... fragmentation of extracted DNA into pieces of an average size of 2 kb by sonication using a Covaris E210 (Massachusetts, USA) followed by end-repair using the End-It end repair kit (Epicentre), iii ...
-
bioRxiv - Immunology 2020Quote: ... which were washed with PBS and resuspended in QuickExtract DNA Extraction Solution (Epicentre, QE09050). Cells were incubated at 68 °C for 15 min and 95 °C for 8 min and the integrated synthetic VL-Ck-2A-VH antibody region was PCR-amplified with flanking primers 5’-CATGTGCCTTTTCAGTGCTTTCTC-3’ and 5’-CTAGATGCCTTTCTCCCTTGACTC-3’ that were specific for the 5’ and 3’ homology arms ...
-
bioRxiv - Microbiology 2020Quote: ... treated with 10 U Plasmid-Safe ATP-Dependent DNase (Lucigen) exonuclease at 37°C for 1 hr ...
-
bioRxiv - Microbiology 2020Quote: ... coli EC100D-pir+ (Lucigen).
-
bioRxiv - Biochemistry 2020Quote: ... genomic DNA was extracted using Quickextract DNA extraction solution (Lucigen) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... Total RNA was extracted according to the manufacturer’s instructions (MasterPure Yeast RNA Purification Kit, Epicentre, MPY03100). Successful plasmid shuffling was confirmed by total RNA extraction and RT-qPCR for rRNA tags ...
-
bioRxiv - Biochemistry 2020Quote: ... cloni®10G chemically competent cells (Lucigen) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... coli C43 (DE3) (Lucigen, Middleton, WI, USA) was used for recombinant gene expressions ...
-
bioRxiv - Bioengineering 2020Quote: ... cloni® 10G (Lucigen) competent cells by electroporation ...
-
bioRxiv - Bioengineering 2020Quote: ... cloni® 10G (Lucigen) competent cells by electroporation ...
-
bioRxiv - Bioengineering 2020Quote: ... genomic DNA was isolated from the transfected cells using DNA QuickExtract™ Buffer (Lucigen) in adherence to the manufacturer’s instructions.
-
bioRxiv - Bioengineering 2020Quote: ... After mutagenesis DNA sublibrary pools were digested with appropriate restriction enzymes to remove phagemid template before transformation into SS320 electrocompetent cell (Lucigen) for phage production ...
-
bioRxiv - Bioengineering 2020Quote: Genomic DNA was extracted with QuickExtract DNA Extraction Solution (catalog no. QE09050; Lucigen, Biosearch technologies ...
-
bioRxiv - Neuroscience 2020Quote: ... Ligated plasmid was then treated with Plasmid-Safe exonuclease (Lucigen), to prevent unwanted recombination products ...
-
bioRxiv - Synthetic Biology 2020Quote: ... coli from Lucigen.
-
bioRxiv - Molecular Biology 2020Quote: ... The ChIP-DNA samples were end repaired using End-It DNA End Repair Kit (Lucigen) and A-tailed using Klenow Fragment and dATP (New England Biolabs) ...
-
bioRxiv - Genomics 2020Quote: ... Libraries for WGBS were prepared as follows: DNA fragments were end-repaired using the End-It kit (Epicentre), A-tailed with Klenow exo-(NEB ...
-
bioRxiv - Genomics 2020Quote: ... Genomic DNA was isolated using QuickExtract DNA Extraction Solution (Lucigen). PCR amplified target sequence was heated to 95°C and slowly (2C/s ...
-
bioRxiv - Microbiology 2020Quote: ... gel purified and circularised using CircLigase (Epicentre) for 1h at 60° C followed by heat inactivation for 10min at 80°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... 100,000 iPSCs were taken for DNA extraction using Quick Extract (Lucigen), whereas organoid samples were directly added to Quick Extract (Lucigen) ...
-
bioRxiv - Developmental Biology 2020Quote: ... whereas organoid samples were directly added to Quick Extract (Lucigen), and vortexed ...
-
bioRxiv - Bioengineering 2020Quote: ... using AmpliScribe T7-Flash Transcription Kit (Lucigen) following the manufacturer’s instruction and purified by RNA Clean & Concentrator (Zymo) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Genotyping PCR was performed using Failsafe PCR 2x PreMix H (Lucigen) and Taq polymerase (NEB).