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Citations for Lucigen :
2001 - 2050 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... with Escherichia coli codon usage was synthesized and subcloned into pSol SUMO vector using Expresso® Solubility and Expression Screening System (Lucigen). A pET16b plasmid encoding the fluorescent protein substrate of Mpro (His10-mTurquoise2-TSAVLQSGFRKM-mVenus ...
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bioRxiv - Biochemistry 2020Quote: ... Beads were resuspended in PCR master mix (EconoTaq PLUS 2× Master Mix, Lucigen), the DNA was amplified for 15 cycles and purified (QIAGEN) ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were incubated with 150 μg/ml RNAse A (Epicentre, Paris, France) for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... cellular genomic DNA was extracted using QuickExtract DNA solution (Epicentre) and the Gaac.1826 target locus was PCR amplified and purified using DNA Clean & Concentrator™ (Zymo Research ...
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bioRxiv - Microbiology 2020Quote: ... with key features being that RNA was rRNA depleted using RiboZero and libraries constructed using ScriptSeq (Epicentre). Samples were sequenced by paired-end sequencing on the HiSeq platform (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... and amplified in Endura cells (Lucigen #60242) as described previously(Joung et al. ...
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bioRxiv - Plant Biology 2020Quote: A BAC library was constructed with pIndigoBAC-5 (Hind III-Cloning Ready) for a heterozygous resistant plant K182 carrying the Rpi-mcq1 followed the instrument (Epicentre, WI, USA). The library is approximately 9× coverage with an average insert size of 85 kb ...
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bioRxiv - Microbiology 2020Quote: ... and rRNA was depleted with the Illumina Ribo-Zero rRNA Removal kit (Epicentre). RNA integrity was checked with an RNA nano chip (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was isolated from obtained clones using QuickExtract (Lucigen), the gRNA-targeted sites PCR amplified and the products Sanger-sequenced ...
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bioRxiv - Microbiology 2020Quote: ... TG1 cells (Lucigen) were transformed with this library by electroporation.
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bioRxiv - Microbiology 2020Quote: Saliva samples were treated with the Quick ExtractTM DNA Extraction Solution (QE, Lucigen) by mixing 50 μl of saliva with 50 μl of the QE reagent and heating for 5 minutes at 95°C ...
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bioRxiv - Microbiology 2020Quote: ... coli C43 (DE3) electro-competent cells (Lucigen) modified here to incorporate a pspA gene knockout using a Lambda Red recombinase strategy (54) ...
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bioRxiv - Plant Biology 2020Quote: ... Products were then circularized with CircLigase (Epicentre) according to the manufacturer’s instructions and used as a template for PCR amplification ...
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bioRxiv - Plant Biology 2020Quote: ... A mixture consisting of 1 μl 10x Circligase reaction buffer (Epicentre), 0.5 μl 1 mM ATP ...
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bioRxiv - Microbiology 2020Quote: ... the RNA fragments were poly(A)-tailed and 5’PPP structures were removed with RNA 5’ Polyphosphatase (Epicentre). The RNA sequencing adapter with the barcodes were ligated to the 5’-monophosphate of the fragments ...
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bioRxiv - Microbiology 2020Quote: ... 6 μl Baseline Zero DNAse (0.1 U/μl, Epicentre Technologies, Madison, WI, USA), 1 μl Benzonase (1 U/μl ...
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bioRxiv - Microbiology 2020Quote: Synthetic RNA H4 (26) was radiolabeled at its 5’ end using T4 polynucleotide kinase (Epicentre) and [γ-32P]ATP (Perkin Elmer) ...
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bioRxiv - Plant Biology 2020Quote: ... Isolation of genomic DNA from leaf powder was performed using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre (Epicentre, Chicago, USA) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Isolation of genomic DNA from leaf powder was performed using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre (Epicentre, Chicago, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Microbiology 2020Quote: ... and a mix of 600 µl of tissue and cell lysis solution and 2 µl Proteinase K from the MasterPure Complete DNA and RNA Purification Kit (Epicentre-Lucigen, Middleton, WI) was added to each sample tube ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was isolated from obtained clones using DNA QuickExtract (Lucigen), the sgRNA-targeted sites PCR amplified and the products Sanger-sequenced ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from using DNA QuickExtract (Lucigen). Amplicons for indel analysis were generated by PCR amplification ...
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bioRxiv - Systems Biology 2020Quote: ... for electroporation into Endura electrocompetent cells (Lucigen). After 1 h recovery at 37°C ...
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bioRxiv - Systems Biology 2020Quote: ... RNA-seq libraries were constructed using ScriptSeq™ v2 RNA-Seq Library Preparation kits (Epicentre Biotechnologies) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Purified RNA (2.5 μg) was used for ribosome depletion using a Ribo-Zero™ Gold Kit (Human/Mouse/Rat) (Epicentre Biotechnologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... mRNAs were purified after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre Biotech., WI). Fluorescently labeled cRNAs derived from these transcripts were used to probe Agilent Mouse V4.0 LncRNA Array containing probes for 22,692 mRNAs (ArrayStar ...
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bioRxiv - Neuroscience 2020Quote: Genomic DNA was extracted with QuickExtract™ DNA Extraction Solution (Epicentre, QE09050). Genomic PCR was carried out using Q5® High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Genetics 2020Quote: ... coli (Lucigen, Middleton, WI). Transformants were pooled and cultured in LB media containing 100ug/mL carbenecillin overnight at 30°C and plasmids subsequently harvested using the Qiagen (Valencia ...
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bioRxiv - Genetics 2020Quote: The DNA isolation was performed on the swim up purified sperm using the MasterPure DNA purification kit (Epicentre Biotechnologies, Madison, WI, USA) as previously described [13] ...
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bioRxiv - Genomics 2020Quote: Phagemid-containing SS320 (Lucigen) cultures were incubated overnight in 2YT medium containing 2% glucose ...
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bioRxiv - Genomics 2020Quote: ... and were used as templates for in vitro transcription using the AmpliScribe SP6 Transcription Kit (Epicentre AS3106). The mRNAs were then capped in a subsequent reaction using the ScriptCap m7G Capping System (Cellscript C-SCCE0625).
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bioRxiv - Systems Biology 2020Quote: ... coli cells (Lucigen) using a GenePulserXCell (Biorad ...
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bioRxiv - Genomics 2020Quote: AmpliScribe SP6 Transcription Kit (Epicentre AS3106).
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bioRxiv - Genomics 2020Quote: ... clones were genotyped using PCR primers outside of the gRNA targeted region (Table S6) to identify clones homozygous for the mariner deletion (DNA QuickExtract Lucigen #QE09050). Clones passing initial genotyping were then further expanded ...
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bioRxiv - Genetics 2020Quote: ... and RNA was extracted from each replicate using the MasterPure Yeast RNA Purification Kit (Epicentre). Integrity ...
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bioRxiv - Genetics 2020Quote: ... DNA collection from cultured cells was performed using QuickExtract (Lucigen QE09050).
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bioRxiv - Immunology 2020Quote: ... cloni® 10G electrocompetent cells (F− mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA (StrR)) were purchased from Lucigen Corporation and were also used for phagemid DNA cloning ...
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bioRxiv - Genetics 2020Quote: ... and a MasterPure™ Yeast DNA Purification Kit (Lucigen). PE150 libraries of the evolved strains were prepared and sequenced by Novogen.
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bioRxiv - Neuroscience 2020Quote: ... and 0.5 U/μL Ampligase (Epicentre) in 1× Ampligase buffer] for 30 mins at 37 °C and 45 mins at 45 °C ...
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bioRxiv - Microbiology 2020Quote: ... Ribosomal RNA was depleted using Ribo-ZERO magnetic kit (Epicentre). The transcripts were fragmented and used as templates to generate strand-specific cDNA libraries by TruSeq Stranded Total RNA LT Sample Prep kit (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... were digested overnight with 10 units of Plasmid-Safe ATP-Dependent DNAse (Lucigen, Middleton, Wisconsin) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cloni Supreme electrocompetent cells (Lucigen), providing > 10,000x coverage of both libraries in total transformants.
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bioRxiv - Molecular Biology 2020Quote: 20 µg RNA from each sample was depleted of rRNA using a Ribo-Zero rRNA Magnetic Kit (Epicentre, St Louis, Missouri, United States) including the optional RiboGuard RNase inhibitor according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: DNA from six PVN from each supplemental tactile stimulation group in the repeated room temperature condition and nest temperature condition (total n = 24) was extracted using the Masterpure Complete DNA/RNA Extraction kit (Epicentre) and 300 ng of DNA was used for bisulfite conversion using the Epitect Fast Bisulfite Conversion kit (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: Genome DNA of transfected N2a cells or tissue cells were extracted by QuickExtract™ DNA Extraction Solution 1.0 (QE09050, Lucigen, Middleton, WI) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... and 50 units of Circligase (Epicentre) was added to 3 μl of cDNA and incubated at 60°C for 2 h and 68°C for 1 h ...
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bioRxiv - Systems Biology 2020Quote: ... and removed stable RNAs by using the RiboZero Gram Positive ribosomal RNA depletion kit (Epicentre). We prepared the sequencing libraries using the TruSeq stranded Total RNA kit (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: The MasterPure dual extraction kit was purchased from Epicentre (MC85200, Wisconsin, USA). The weight of each sample is within the range of 2-5 mg according to the manufacture’s guidelines (Supplemental Table 1) ...
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bioRxiv - Molecular Biology 2020Quote: DNA was extracted using the MasterPure Complete DNA and RNA Purification Kit (Epicentre) following manufacturer’s instructions ...