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Citations for Lucigen :
1951 - 2000 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... about 15 μg of total RNA of human frontal cortex were treated with 10 units of RNase R (RNR07250, Epicentre) for 1 hour at 37 °C and purified by phenolchloroform extraction ...
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bioRxiv - Neuroscience 2020Quote: ... coli 5-alpha Chemically Competent cells (Lucigen), performing a thermal shock for 45 seconds at 42°C followed by 2 minutes on ice ...
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bioRxiv - Neuroscience 2020Quote: PCR products were amplified in 100 ml reactions with 0.2 units of EconoTaq DNA polymerase (Lucigen, Middleton, Wisconsin), 1 μM of each primer ...
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bioRxiv - Microbiology 2020Quote: ... bacterial genomic DNA was prepared using the MasterPure™ Gram Positive DNA Purification Kit (Lucigen) and fragmented by AatII digestion and the blot was probed with btl19-13 ...
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bioRxiv - Microbiology 2020Quote: ... with the Ribo-Zero rRNA removal kit for Bacteria (Epicentre) according to standard protocol ...
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bioRxiv - Molecular Biology 2020Quote: A freshly inoculated cotton dry swab (nerbe plus GmbH, 09-819-5000) is inserted into 500 μl of QuickExtract (Lucigen, QE09050) supplemented with 2 ng/μl RNase-free plasmid DNA (pX330 ...
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bioRxiv - Molecular Biology 2020Quote: Quick extract DNA extraction solution (Lucigen) was tested in accordance with the manufacturer’s suggested buffer to sample ratio ...
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bioRxiv - Biochemistry 2020Quote: ... pNT-15 was constructed using NEB HiFi DNA Assembly with a gBlock® from IDT (Coralville, IA) and cloning vector pSMART-HCKan (Lucigen, #40704-2). All PCR reactions were performed with Q5® Hot-Start High Fidelity Master Mix (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... cloni (Lucigen, Middleton WI) was used for cloning and plasmid propagation ...
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bioRxiv - Biochemistry 2020Quote: ... Eluted RNA was again treated with DNase using the Baseline-ZERO DNase kit (Lucigen, catalog # DB0715K) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The standard PCR reaction was performed with the HNqPCRrev1 and HNqPCRfrw1 primers using the Failsafe PCR system with the Premix A (Lucigen, catalog # F599100). Positive and negative PCR control reactions with or without 50ng of HNDNA ...
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bioRxiv - Genetics 2020Quote: ... and ribosomal RNA was depleted using the Ribo Zero Gold Magnetic system (Epicentre/Illumina, San Diego, CA). RNA-seq libraries were prepared with the ScriptSeq v2 kit (Epicentre ...
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bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared with the ScriptSeq v2 kit (Epicentre) and subjected to paired-end sequencing (2 x 100 bp ...
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bioRxiv - Bioengineering 2020Quote: ... in 10 μL PCR reactions consisting of 5μL of 2X EconoTaq Master mix (Lucigen), 1uL of each primer (10μM) ...
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bioRxiv - Bioengineering 2020Quote: ... using 2X EconoTaq Master mix (Lucigen) in 10 μL PCR reactions consisting of 5μL of 2X EconoTaq Master mix (Lucigen) ...
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bioRxiv - Bioengineering 2020Quote: ... Biopsies were transferred to 10μL of Epicenter DNA extraction buffer (Lucigen, Palo Alto, CA) and lysed as described above ...
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bioRxiv - Bioengineering 2020Quote: ... Embryos that reached blastocyst stage were lysed in 10μL of Epicenter DNA extraction buffer (Lucigen, Palo Alto, CA) using a SimpliAmp Thermal Cycler (Applied Biosystems ...
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bioRxiv - Bioengineering 2020Quote: ... 1.5 U/µl NextGen T7 RNA polymerase (Lucigen), 1 mM of each rNTP (NEB) ...
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bioRxiv - Bioengineering 2020Quote: ... 2’-fluoro (2’-F) modified RNA was synthesized using the DuraScribe T7 transcription kit (Lucigen) as described in the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... Genomic DNA was extracted from wild-type and GLUT1-null Caco-2 cells with QuickExtract DNA Extraction Solution (Lucigen, SS000035-D2) and regions of interest were PCR amplified with the designed primers and Taq polymerase (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... PP contamination was removed using the Plasmid Safe ATP-dependent DNase kit (Epicentre, WI, USA), and the products where cleaned and concentrated using the Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Bioengineering 2020Quote: ... cell pellets were resuspended in a QuickExtract DNA extraction solution (Lucigen, Middleton, WI, USA), incubated at 65°C for 10 mins ...
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bioRxiv - Biochemistry 2020Quote: ... cloni® 10G Electrocompetent Cells (Lucigen) (> 5 x109 cfu/μg ...
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bioRxiv - Biochemistry 2020Quote: ... coli C43(DE3) strain (Lucigen, WI). Protein expression was induced by 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2020Quote: ... coli Cloni® 10G cells (Lucigen, USA; 1ul crude reaction mix). Kanamycin selected clones were screened by colony PCR and verified by sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C (1 – 2 h) and 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and sequenced.
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bioRxiv - Biochemistry 2020Quote: ... 1 μl from PCR reaction aliquots were transformed in electrocompetent E.coli Cloni® 10G cells (Lucigen, USA). Colonies were screened by colony PCR and positive colonies were sequenced ...
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bioRxiv - Biochemistry 2020Quote: ... Wild-type proteins were expressed in strain C41 (Lucigen) by induction at mid-log phase (OD600∼0.4 ...
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bioRxiv - Biochemistry 2020Quote: ... Mutant proteins were expressed in strain C41 (Lucigen) by induction at mid-log phase (OD600 ∼0.4 ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from each isolate using QuickExtract (Lucigen, Wisconsin, USA) according to manufacturer’s protocol from the above mentioned 0.1X TSB cultures ...
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bioRxiv - Neuroscience 2020Quote: ... and Failsafe buffer J (Epicentre Biotechnologies) at a final concentration of 1X ...
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bioRxiv - Neuroscience 2020Quote: ... Following amplification with EconoTaq PLUS GREEN 2X Master Mix (Lucigen), products were agarose gel-purified to select single strand DNA using the QiaQuick Gel Extraction kit (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: ... and transferred to tubes containing 3 μL of lysis buffer (Epicentre, MessageBOOSTER kit). Cells were collected within 1 h of removal from the incubator and within 4 h of removal from the animals.
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bioRxiv - Neuroscience 2020Quote: The RNA isolated from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, Message BOOSTER kit for cell lysate), run through RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: ... Tobacco Acid Pyrophosphatase (Epicentre, discontinued) or recombinant PIR-1 was used to dephosphorylate ppp-RNAs for cloning ppp-RNAs when needed while no such treatment was required for cloning p-RNAs ...
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bioRxiv - Biochemistry 2020Quote: ... Illumina adapters were ligated using Circ Ligase I (Lucigen) and linker pA-Adapt-Bp (Table S5) ...
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bioRxiv - Biophysics 2020Quote: ... Rolling circle amplification was performed by using phi29 polymerase (Lucigen, 30221) for 12 hours at 37°C followed by 2 washes each 5 min with label probe hybridization buffer (2x SSC ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was harvested using QuickExtract solution (screening of CRISPR/Cas9 clones; Epicentre EPI-QE0905T) or NucleoSpin Tissue kit (routine genotyping ...
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bioRxiv - Neuroscience 2020Quote: ... transformed into E.coli TG1F+ DUO (Lucigen) with a minimal library size of 3E+08 transformants per HCDR3 length and phages were produced using VCSM13 helper phage (Agilent Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... transformed into E.coli TG1F+ DUO (Lucigen) with a minimal library size of 1E+08 transformants ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... QuickExtract DNA Extraction Solution was purchased from Lucigen Corporation (WI ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasmid-Safe ATP-Dependent DNase was purchased from Epicentre Technologies Corp ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Step 2: Riboshredder RNase (Epicentre) was used in place of RNase A ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic DNA from infected barley leaves at 6 days post-inoculation (dpi) was isolated by using the MasterPure™ Complete DNA&RNA purification Kit (Epicentre®, Illumina®) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The gDNA was isolated by using a MasterPure™ Complete DNA&RNA Purification Kit (Epicentre®, Illumina®, Madison, Wisconsin, USA) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... cloni® 10G (F-mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ-tonA) produced by Lucigen (Middleton, WI, USA). Genes encoding for OMTs selected for in vivo testing were cloned directly from the synthetic DNA fragments by restriction and ligation (NcoI/XhoI ...
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bioRxiv - Biochemistry 2020Quote: ... C-terminally truncated MCM9 constructs (643-900 or 680-900) with GST at the N-terminus were transformed into C43 pLysS (Lucigen, Middleton, WI) and induced with 0.25 mM IPTG at OD600 ~ 0.5 for 4 hours at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Cloni 10G ELITE cells (Lucigen) in 1.0-mm Bio-Rad cuvettes using a Gene Pulser Xcell Electroporation System (settings ...
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bioRxiv - Biochemistry 2020Quote: ... coli (Lucigen) in 1.0-mm Biorad cuvettes using a Gene Pulser Xcell Electroporation System (settings ...
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bioRxiv - Biochemistry 2020Quote: ... Linker scanning mutagenesis was performed with an Ez-Tn5 kit (Epicentre®) as previously described49.