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Citations for Lucigen :
1851 - 1900 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and 10-U endonuclease (Lucigen Cat#DB0715K) for 30-min at 37°C and then incubated at 65°C for 10-min to stop the digestion ...
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bioRxiv - Molecular Biology 2020Quote: ... Single colonies of each suppressor strain were obtained and genomic DNA was prepped using MasturePure(tm) Yeast DNA Purification Kit (Lucigen). The DHR1 ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR screening of the colonies was performed using genotyping oligos listed in Supplementary Table 1 using Quick Extract DNA Extraction Solution (Lucigen Catalog Number: QE09050) according to manufacturer’s protocol in a PCR machine and DreamTaq Green Polymerase (Thermo Fisher Scientific Catalog Number ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were then removed in the magnetic field and RNase treatment (5µg/µl Epicentre MRNA092) performed for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNAs were size-purified on TBE-Urea gels before being circularized by CircLigase II (Epicentre). Circularised cDNAs were then annealed to an oligonucleotide complementary to the BamHI site and then BamHI digested ...
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bioRxiv - Molecular Biology 2020Quote: ... Whole cell lysates containing 10 µg of total RNA were each treated with 12.5 units of RNase I (Epicentre) at 23°C for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... whole cell lysates containing 20 µg of total RNA were each treated with 10 units of RNase I (Epicentre) at 24°C for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Recovered cDNA was circularized with Circligase™ II (Epicentre) at 60 °C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... phosphorylated DNA using the End-It DNA End-Repair Kit (Lucigen). DNA was purified using ratio of 1:1.8 sample to AMPure XP beads ...
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bioRxiv - Molecular Biology 2020Quote: ... HSPCs were harvested and QuickExtract DNA extraction solution (Epicentre, Madison, WI, USA) was used to collect gDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... HSPCs were harvested and QuickExtract DNA extraction solution (Epicentre, Madison, WI, USA) was used to collect gDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μg of total RNA was subjected to RNase R treatment (29) with 5U RNase R (Lucigen, RNR07250) at 37°C for 30 min and then placed on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... The small RNA fraction was treated with 20U of RNA 5’ Polyphosphatase (TAP, Lucigen), in a 50μl reaction at 37°C for 1h ...
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bioRxiv - Molecular Biology 2020Quote: Rolling Cycle Amplification (RCA) was done O/N at 30°C using 0.5 Units/μl Φ29 polymerase (Lucigen, 30221-2). The reaction mixture contained also 1X Φ29 buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... were mounted on the slides and sections were preconditioned for 30 minutes at room temperature (R/T) with hybridization-reaction mixture of 1X Ampligase Buffer (Lucigen, A1905B), 0.05M KCl (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... by annealing locus-specific oligonucleotides to a common 5’ universal oligonucleotide and performing in vitro RNA transcription (AmpliScribe T7-Flash Kit, Lucigen, ASF3507)40 ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was extracted from modified cell lines using QuickExtract DNA extraction buffer (Epicentre Technologies, Madison, Wisconsin, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... media was aspirated and 50-100 μL QuickExtract™ DNA Extraction Solution (Lucigen) were added to each well ...
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bioRxiv - Genomics 2020Quote: ... an aliquot of 25 uL was reserved from each sample for extraction of DNA with MasterPure™ kit (Epicentre) and bacterial cell count was measured using qPCR (TaqMan™) ...
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bioRxiv - Genomics 2020Quote: ... electroporated in triplicate into Endura electrocompetent cells (Lucigen, Middleton WI), and plated onto 24.5 cm2 LB-agar plates ...
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bioRxiv - Genomics 2020Quote: ... cDNA was circularized with CircLigase (Epicentre, CL4111K). DNA libraries were amplified by PCR for 6-10 cycles with Phusion HF DNA Polymearse (NEB ...
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bioRxiv - Genomics 2020Quote: ... 2.5 μL of T4 polynucleotide kinase (5 U/μL) plus 2.5 μL 10X Reaction Buffer (Lucigen) were added to the initial 20-μL nucleic acids solution ...
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bioRxiv - Microbiology 2020Quote: Transposon mutagenesis was performed using the EZ-Tn5™
Tnp Transposome™ Kit (Epicentre), according to manufacturer’s specifications ... -
bioRxiv - Genomics 2020Quote: ... The End-It DNA End-Repair Kit (Epicentre) was used to repair DNA fragments to blunt ends ...
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bioRxiv - Genomics 2020Quote: ... They were then amplified using electroporation in Endura electrocompetent cells (Lucigen), to achieve at least 250 colonies per sgRNA in the library ...
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bioRxiv - Genomics 2020Quote: T7 RNA polymerase (Lucigen NxGen)
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bioRxiv - Genomics 2020Quote: Libraries were prepared from the DNA samples using the NxSeq AmpFREE Low DNA Library kit (Lucigen, Cat no. 14000-2) as per the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... washed with PBS and resuspended in 200 μL of Quick Extract (Lucigen) lysis buffer and cells were lysed according to the manufacture’s protocol ...
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bioRxiv - Genomics 2020Quote: ... 50 µL of Quick Extract (Lucigen) was added to the cell pellet and the cell mixture was transferred to a 96-well PCR plate (Bio-Rad) ...
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bioRxiv - Genomics 2020Quote: ... Library preparation was performed following the manufacturer’s protocol using the Illumina ScriptSeq™ v2 RNA-Seq Library Preparation Kit (Epicentre, USA). Quality of the RNA-Seq transcriptome library was examined using Agilent 2100 High Sensitivity DNA Kit ...
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bioRxiv - Genomics 2020Quote: RNA samples with RNA Integrity Number (RIN) >7.0 were chosen to proceed to rRNA depletion using Ribo-Zero™ rRNA Removal Kits (Bacteria) (Epicentre, USA) prior to cDNA synthesis ...
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bioRxiv - Genomics 2020Quote: We extracted high molecular weight (HMW) genomic DNA using the Masterpure Complete DNA and RNA purification kit (Lucigen), using the protocol for tissue samples ...
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bioRxiv - Genomics 2020Quote: ... gDNA for the rest of the strains was extracted using the Masterpure Complete DNA and RNA purification kit (Lucigen) using the protocol for tissue samples ...
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bioRxiv - Genomics 2020Quote: Genomic DNA from 300 million cells was purified using the Master Pure Yeast DNA Purification kit (Epicentre) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... 1ug of RNA was incubated with 5U of RNaseR (Epicentre) for 45 min at 37C in a 14ul reaction volume to digest all linear RNA ...
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bioRxiv - Genomics 2020Quote: ... both transcripts were biotin-labeled after in vitro transcription from 1µg linearized pcDNA3.1-LETR1-1 and pcDNA3.1-LETR1-1-antisense plasmids for 1h at 37°C using Ampliscribe T7-flash biotin-RNA kit (Lucigen). Biotinylated LETR1 sense and antisense RNA were then treated with RNase-free DNase I for additional 15min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... RNA extraction was done using the ‘MasterPure Yeast RNA Purification Kit’ (Epicentre) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... using a Gibson assembly master mix (New England Biolabs).Gibson assembly products were transformed into electrocompetent cells (E. cloni, Lucigen) and plated on 245mm x 245mm square LB-agar plates to obtain the sufficient number of bacterial colonies at a ∼50× library coverage ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was end-repaired using End-it from Lucigen. 17.5ul DNA was added to 2.5ul 10X end-it buffer ...
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bioRxiv - Genomics 2020Quote: ... 400 ng of pooled plasmid DNA was electroporated into Endura (Lucigen) E ...
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bioRxiv - Genomics 2020Quote: ... 2 mL of recovery buffer (Lucigen) was added ...
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bioRxiv - Genomics 2020Quote: ... then digested with exonuclease I by adding 1 μL of enzyme (20 U/μL) plus 1.5 μL 10X Reaction Buffer (Lucigen) and incubating 1 h at 37 °C ...
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bioRxiv - Genomics 2020Quote: ... Nucleic acids were extracted using MasterPure yeast DNA purification kit (Epicentre, MPY80200) according the manufacturer’ instructions ...
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bioRxiv - Genomics 2020Quote: To test the Terminator™ 5’-phosphate-dependent exonuclease (Lucigen, Cat. # TER5120), 100 ng of total RNA in 2 μL was combined with 18 μL TEX mix ...
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bioRxiv - Genomics 2020Quote: ... The plugs were washed and then solubilized with GELase (Epicentre). The purified DNA was subjected to 45min of drop-dialysis ...
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bioRxiv - Genomics 2020Quote: ... and rRNA depletion was performed using Ribo-Zero Magnetic Kit H/M/R (Epicentre/Illumina).
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bioRxiv - Microbiology 2020Quote: ... Ready-lyse lysozyme and Omnicleave nuclease (Epicentre) were added and the mixture was permitted to rock on ice for 15 minutes ...
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bioRxiv - Bioengineering 2020Quote: The 5′-biotinylated-E07 (anti-EGFR aptamer) was generated by performing an in vitro transcription reaction (DuraScribe T7 Transcription Kit, Lucigen, #DS010925), as described previously (Ray et al. ...
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bioRxiv - Synthetic Biology 2020Quote: ... All strains were constructed using Escherichia coli strain C43(DE3) (Lucigen, Madison, WI). The deletion of frdABCD ...
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bioRxiv - Microbiology 2020Quote: ... RNA was directly treated with Baseline-ZERO DNase (2.5 units/50 µL; Epicentre) for 30 min at 37°C ...