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Citations for Lucigen :
2151 - 2200 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Aliquots from these lysates were used for a PCR (with a Taq DNA polymerase with standard Taq buffer NEB or EconoTaq DNA polymerase Lucigen) with respective gene primers and an 18mer M13F-FAM fluorescent tagged primer (5’-TGTAAAACGACGGCCAGT-3’ ...
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bioRxiv - Synthetic Biology 2020Quote: ... 22 Alanine production plasmids were constructed using codon optimized (Codon Optimization Tool from the IDT) synthetic DNA and the pSMART-HCKan vector (Lucigen, Middleton, WI). Plasmids were assembled using NEBuilder® HiFi DNA Assembly Master Mix following manufacturer’s protocol (NEB ...
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bioRxiv - Systems Biology 2020Quote: ... the fragmentation of 100 pg cDNA was performed with EZ Tn5 Transposase (Lucigen, TNP92110). Fragments of cDNA were amplified by KAPA HiFi hotstart readymix (EMSCO/FISHER ...
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bioRxiv - Systems Biology 2020Quote: ... Total-RNA (250ng) was depleted of ribosomal RNA using RNaseH (Epicentre) and ribosomal RNA targeting oligonucleotides based on a protocol by Adiconis et al. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... shRNAs were synthesized with AmpliScribe™ T7-Flash™ Transcription Kit (Epicentre, Charlotte, NC, USA) for 15 h followed by 15 min treatment with 1 μl of DNAse I ...
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bioRxiv - Synthetic Biology 2020Quote: ... and genomic DNA was extracted using 50 μL of Quick Extract DNA Extract Solution (Lucigen, QE09050) per well according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... coli (Lucigen). Aliquots of cells were spread onto 2YT agar plates supplemented with ampicillin and glucose ...
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bioRxiv - Microbiology 2020Quote: ... -1B and -1C were each cloned into PADL22c vector (Lucigen) with a N-terminal PelB leader sequence and C-terminal His tag and HA tag ...
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bioRxiv - Microbiology 2020Quote: ... coli (Lucigen), SHuffle T7 E ...
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bioRxiv - Microbiology 2020Quote: ... coli (Lucigen), TG1 E ...
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bioRxiv - Microbiology 2020Quote: ... 16S rRNA was minimized using the Ribo-Zero rRNA removal kit for bacteria (Epicentre, Madison, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by chromosomal DNA isolation (Quick Extract /Lucigen), PCR amplification (Sup Table 8 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and cm genes with vector Pmod2-MCS (Epicentre) via Gibson Assembly ...
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bioRxiv - Microbiology 2020Quote: ... and 0.1 µl Ready-Lyse™ Lysozyme solution (Epicentre) and incubated for 1 hour at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was spun down at 5,000 x g for 5 minutes and the pellet was resuspended in 100 µl of QuickExtract™ DNA Extraction Solution (Lucigen) and 0.1 µl Ready-Lyse™ Lysozyme solution (Epicentre ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcription of shRNAs was performed using the AmpliScribe™ T7-Flash™ Transcription Kit (Lucigen). The transcription reaction was allowed to continue at 37°C for 5-7h ...
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bioRxiv - Genomics 2020Quote: ... Yeast total RNA was extracted with the MasterPure Yeast RNA Purification Kit (Lucigen MPY03100) as per the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: ... and 23S rRNA subunits and spaced nine bases apart were then annealed at 65 °C followed by digestion with Hybridase (Lucigen), a thermostable RNase H ...
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bioRxiv - Systems Biology 2020Quote: ... RNA digestion of lysates was performed for 1 hour with the mixture of 2000 Units of RNase T1 (Epicentre) and 300 Units of RNase S7 (Roche/Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl RNase inhibitor (Epicentre), 1X NEB buffer 3.1 (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl RNase inhibitor (Epicentre), and nuclease-free water (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 u/µl NxGen RNase inhibitor (Lucigen). Control reactions contained 320 µg/ml puromycin (Santa Cruz Biotechnology ...
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bioRxiv - Synthetic Biology 2020Quote: Transposon Cassettes were inserted into pNL4-3 by in vitro transposition with EZ-Tn5 transposase (Epicentre) per manufacturer’s protocol and with equal mols of plasmid template and transposon ...
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bioRxiv - Biophysics 2020Quote: ... cloni EXPRESS BL21 (DE3) cells (Lucigen Corporation, Middleton, USA) were transformed with a pT7 plasmid containing the ClyA-AS gene ...
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bioRxiv - Microbiology 2020Quote: ... Three μg of purified DNA were treated with 2 μl of Plasmid-Safe ATP-dependent exonuclease (Epicentre, Madison, USA) at 37°C for 30 minutes and then heat-inactivated by a 30-min incubation at 70°C.
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bioRxiv - Cancer Biology 2020Quote: ... BACs were isolated from 50ml bacterial cultures using the BACMAX Bac extraction kit (Epicentre). Isolated BACs were sonicated (x10 cycles of 15 seconds ‘on’ 45 seconds ‘off’ at constant intensity with power set to ‘3’ ...
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bioRxiv - Synthetic Biology 2020Quote: ... and C41(DE3) (Lucigen; Wisconsin, USA) were used for protein expression.
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bioRxiv - Genetics 2020Quote: ... Genomic DNAs were extracted by QuickExtract (Epicentre) solution and amplified by Phusion High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Genetics 2020Quote: ... the linear RNA was digested with 3U of RNase R (Epicentre, USA) per μg of RNA ...
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bioRxiv - Genetics 2020Quote: ... ribosomal RNA was removed by Epicetre Ribozero™ rRNA Removal Kit (Epicentre, USA), and rRNA free residue was cleaned up by ethanol precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... was transformed into electrocompetent Endura cells (#60242, Lucigen) in quadruplicates ...
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bioRxiv - Evolutionary Biology 2020Quote: Illumina fragment libraries (insert sizes 270b and 420b) and mate-pair libraries (insert sizes 2kb, 4kb and 8kb) were constructed by Lucigen Corporation (Middleton ...
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bioRxiv - Molecular Biology 2020Quote: Strand specific libraries were prepared from ribosomal-RNA depleted mRNA using ScriptSeq Complete Plant kit (Epicentre) and sequenced on an Illumina HiSeq2500 ...
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bioRxiv - Molecular Biology 2020Quote: ... Strand specific libraries were prepared from ribosomal-RNA depleted mRNA using ScriptSeq Complete Plant kit (Epicentre) and sequenced on the Illumina HiSeq2500 platform (100 bp single-end reads) ...
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bioRxiv - Molecular Biology 2020Quote: Transposon mutant libraries were constructed using the EZ-Tn5
TnP Transposome Kit (Epicentre) according to the manufacturer’s instructions ... -
bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Molecular Biology 2020Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using MasterPureTM Yeast DNA purification kit (Lucigen/Epicentre). Genomic DNA of Ch16-Tel-DSB ...
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bioRxiv - Molecular Biology 2020Quote: ... 500 ng of total RNA were depleted of ribosomal RNA using the Ribo-Zero rRNA removal kit (Epicentre). The resulting RNA was then used for library preparation using the TruSeq small RNA Sample Prep Kit (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was resuspended in 7 μl of water and then 8 μl of CircLigase II mix (5 μl water, 1.5 μl 10x CircLigase Buffer II, 0.75 μl 50 mM MnCl2, 0.75 μl Circligase II [Lucigen]) were added ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was then incubated at 37 °C for 1 h with 20 U of RNase R (Lucigen, cat n°RNR07250), to remove all the undesired products (i.e linear RNA or concatemer product) ...
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bioRxiv - Molecular Biology 2020Quote: ... The swabs were each placed in 1.5 mL Eppendorf tube containing 200 ul of QuickExtract DNA Extraction Solution (QE buffer, Lucigen LLC, Madison, WI). Each tube was vortexed and stored until extraction.
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl RNase R mixture (Epicentre) was added to the sample before incubation at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... furfur CBS 14141 were isolated with MasterPure yeast DNA kit (Epicentre) as per manufacturer’s protocol with additional step of homogenizing at 6 m/s for 50 sec (MP Biomedicals) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA template was incubated at 37°C overnight in a transcription mix containing: transcription buffer 1X (Epicentre Biotechnologies), 1 mM 2’F-Py (2’F-2’-dCTP and 2’F-2’-dUTP ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1.5 u/μl of the mutant T7 RNA polymerase (T7 R&DNA polymerase, Epicentre Biotechnologies). After transcription ...
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bioRxiv - Synthetic Biology 2020Quote: ... Electroporations were recovered in 1 mL recovery media (Lucigen) for 1 hour and subsequently grown overnight in LB + selection.
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bioRxiv - Synthetic Biology 2020Quote: ... coli (Lucigen 10G Elite) cells using 0.1 cm electroporation cuvettes (GenePulser cuvettes ...
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bioRxiv - Genetics 2020Quote: ... Genomic DNA (gDNA) was extracted with QuickExtract buffer (Lucigen, Middleton, USA) by adding 30 μL to a well of a 96-well plate and incubating for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA for clone screens was extracted by QuickExtract (Lucigen) or ethanol precipitation.
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bioRxiv - Genomics 2020Quote: ... QuickEx (Lucigen) was used for rapidly extracting genomic DNAs from the transfected cells according to manufacturer’s protocol ...