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Citations for Lucigen :
2051 - 2100 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... using genomic template DNA extracted using QuickExtract DNA extraction solution (Epicentre/ Lucigen, Wisconsin, USA). DNA was prepared by harvesting the cells in the QuickExtract DNA extraction solution and heating the samples at 65⁰C for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... using genomic template DNA extracted using QuickExtract DNA extraction solution (Epicentre/ Lucigen, Wisconsin, USA). DNA was prepared by harvesting the cells in the QuickExtract DNA extraction solution and heating the samples at 65⁰C for 15 min ...
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bioRxiv - Microbiology 2020Quote: ATCC 17978 transposon mutants were generated using the EZ-Tn5™
Insertion Kit (Epicentre Biotechnologies) as previously described [33] ... -
bioRxiv - Microbiology 2020Quote: ... GU332722.1) or Asia II 1 (GenBank accession no. GU332721.1) was synthesized using the AmpliScribe T7- Flash Transcription Kit (Epicentre, ASF3507), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The genomic DNA from the mycelia was extracted using the MasterPure™ Yeast DNA Purification kit (Lucigen) and used subsequently for PCR amplification ...
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bioRxiv - Microbiology 2020Quote: Escherichia coli CopyCutter EPI400 (Lucigen) was used for cloning of the pbp2m gene and was grown in Luria-Bertani (LB ...
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bioRxiv - Microbiology 2020Quote: ... HBV cccDNA was quantified from total DNA following digestion for 45 min at 37°C with T5 exonuclease (Epicentre) to remove rcDNA followed by 30 min heat inactivation ...
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bioRxiv - Genomics 2020Quote: ... Multiple PCR reactions for each sample were carried out with 200 pg of annealed DNA using the XpYpE2 and teltail primers (Supplementary Table 3) and FailSafe PCR reagents (Epicentre). PCR conditions were as follows ...
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bioRxiv - Genomics 2020Quote: ... and NxSeq AmpFREE Low DNA Library Kit (Lucigen) was applied ...
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bioRxiv - Cancer Biology 2020Quote: ... The genomic DNA from individual cell clones was extracted using QuickExtract DNA Extraction Solution (Lucigen) and screened by PCR for the deletion amplicon using the DNA primers listed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg RNA was used for reverse transcription with 0.5 μl MMLV HP reverse transcriptase (Lucigen cat # RT80125K) per 20 μl rxn ...
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bioRxiv - Cell Biology 2020Quote: ... strand-specific RNA-Seq libraries facilitating multiplexed paired-end sequencing were prepared from 500 ng total-RNA using the Ribo-Zero Magnetic Gold technology (Epicentre, an Illumina company) for depletion of rRNA followed by library preparation using the ScriptSeq v2 technology (Epicentre) ...
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bioRxiv - Cell Biology 2020Quote: ... for depletion of rRNA followed by library preparation using the ScriptSeq v2 technology (Epicentre). The RNA-Seq libraries were combined into 2 nM pooled stocks ...
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bioRxiv - Bioengineering 2020Quote: ... Embryos that reached blastocyst stage were lysed in 10μL of Epicenter DNA extraction buffer (Lucigen, Palo Alto, CA) using a SimpliAmp Thermal Cycler (Applied Biosystems ...
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bioRxiv - Bioengineering 2020Quote: ... for the top four guides for construction of the gRNA and were used for in vitro transcription using the AmpliScribe T7-Flash Transcription kit (Lucigen, Palo Alto, CA) and purified using the MEGAclear Transcription Clean-Up kit (Thermo Fisher ...
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bioRxiv - Biophysics 2020Quote: Genomic DNA was isolated from candidate cell lines using the QuickExtract DNA Extraction Solution (Lucigen, #QE09050) essentially as described ...
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bioRxiv - Cell Biology 2020Quote: ... coli 10G chemically competent cells (Lucigen). Plasmids were isolated from transformants and Sanger sequencing was used to identify those containing the correct insertion.
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA was isolated with the MasterPure™ Yeast DNA Purification Kit (Lucigen) and submitted to GENEWIZ for whole-genome sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... 1.25 OD units of each sample was resuspended in 50 μl PBS containing 400 units of ReadyLyse lysosome (Epicentre), 12.5 units benzonase (Sigma ...
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bioRxiv - Genomics 2020Quote: ... ethanol precipitated and transformed into 200l of electrocompetent E.coli (Lucigen Endura™ ElectroCompetent Cells ...
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bioRxiv - Genomics 2020Quote: ... ethanol precipitated and transformed into 200l of electrocompetent E.coli (Lucigen Endura™ ElectroCompetent Cells, Lucigen). 4 x 250ml LB preps (supplemented with 100 μg mL-1 carbenicillin ...
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bioRxiv - Neuroscience 2020Quote: ... using Inpp5e WT forward primer 5’-GAGAAGCTGATAGATGGCTAGG and Inpp5e WT reverse primer 5’-AACCAGAAGACCTCATCAAACC and EconoTaq PCR according to manufactures specifications (Lucigen corporation, WI) (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... genomic DNA was extracted with the QuickExtract kit (Epicentre, #QE09050). PCR was performed using Q5 Hot Start High-Fidelity 2X Master Mix ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA for whole genome sequencing was extracted using the MasterPure™ Yeast DNA Purification kit (Lucigen, US) following the manufacturers protocol ...
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bioRxiv - Microbiology 2020Quote: ... followed by circularization with CircLigase (Epicentre). Finally ...
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bioRxiv - Microbiology 2020Quote: ... rRNA was removed using a Ribo-Zero Magnetic kit (Epicentre Inc., USA) from each 5 ug of total RNA ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatants were used as DNA templates for polymerase chain reactions (PCRs, EconoTaq Plus Green 2X mater mix, Lucigen, Middleton, WI, USA). The genotyping primers were as described previously29,30,92
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bioRxiv - Developmental Biology 2020Quote: ... and Sanger sequencing was then extracted using QuickExtract™ DNA Extraction Solution (Lucigen). The PDX1 knockout line was generated in an analogous way.
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bioRxiv - Developmental Biology 2020Quote: ... Cell debris was then pelleted and used for gDNA extraction with 10-20 µl QuickExtract™ DNA Extraction Solution (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... coli into pETite vector (Lucigen, USA) through enzyme-free method ...
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bioRxiv - Microbiology 2020Quote: ... packaged into lambda phage and transferred into Eco EC100 (Lucigen). Each library was expanded to contain 20 x106 unique cosmid clones with ∼30-45 kb eDNA (environmental DNA ...
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bioRxiv - Microbiology 2020Quote: ... following the manufacturer’s instructions (Epicentre, Illumina Inc., Madison, Wisconsin). Final DNA was resuspended in EB buffer ...
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bioRxiv - Molecular Biology 2020Quote: The >200nt RNA fraction was ribo-depleted using non-overlapping DNA oligonucleotides that are complementary to rRNA 18s and 28s followed by digestion with Hybridase™ Thermostable RNaseH (Lucigen #H39500), as previously described (79) ...
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bioRxiv - Molecular Biology 2020Quote: ... and gel-purified RT products circularized using CircLigase II (Lucigen, CL4115K). rRNA depletion was performed using biotinylated oligos as described in (Ingolia et al ...
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bioRxiv - Molecular Biology 2020Quote: ... The ligation reaction above was then treated with PlasmidSafe (Epicentre, Cat#: E3101K) to digest any residual linearized DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Purification and precipitation of RNA were performed using the MasterPure RNA Purification Kit from Epicentre by adding 200 μl of 2X T and C Lysis Solution and following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... In vitro transcribed RNA were incubated for 1h at 37°C after addition of 10units DNase I and 10units DNase Zero (Epicentre). The RNAs were then ethanol precipitated and resuspended in water ...
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bioRxiv - Molecular Biology 2020Quote: ... Ligated RNAs were then purified using phenol/chloroform extraction followed by ethanol precipitation and then digested using 10U RNase R from Epicentre as described in the RNase R treatment paragraph ...
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bioRxiv - Molecular Biology 2020Quote: ... we used the MasterPure RNA purification kit from Epicentre instead of phenol/chloroform extraction to purify RNA after the dephosphorylation and ligation steps.
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bioRxiv - Molecular Biology 2020Quote: ... In vitro transcribed RNA were incubated for 1h at 37°C after addition of 10units DNase I and 10units DNase Zero (Epicentre). The RNA were then ethanol precipitated and resuspended in water and dephosphorylated using 2units Alkaline Phosphatase (FastAP ...
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bioRxiv - Molecular Biology 2020Quote: ... and 8U of DNase I (Epicentre). These diluted nuclear extracts were pre-cleared using 25µL of previously blocked Streptavidin Sepharose beads (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 2µL Riboguard (Epicentre) and incubated for 30min at room temperature with 500ng of in vitro synthesized RNAs ...
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bioRxiv - Molecular Biology 2020Quote: ... genomic DNA was harvested using QuickExtract (Lucigen), and was saved until library preparation and sequencing.
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bioRxiv - Molecular Biology 2020Quote: Harvested cells were lysed using QuickExtract DNA Extraction Solution (Lucigen, Cat. No. QE09050) following manufacturers protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Metatranscriptomic libraries were prepared for sequencing with the addition of 5–50 ng of RNA to the ScriptSeq cDNA V2 library preparation kit (Epicentre). Metatranscriptomic samples were sequenced with an Illumina NextSeq 500 system using V2 high output 300 cycle reagent kit with PHIX control added ...
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bioRxiv - Microbiology 2020Quote: ... Free mRNA and rRNA ends were then ligated into circular forms with a circRNA ligase (Lucigen); it favors ligation events between proximal RNA ends11 and it has limited activity at high temperature ...
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bioRxiv - Microbiology 2020Quote: ... reactions were set up by adding 0.5 uL of RNaseR (Lucigen), 2.5 uL of RNase R buffer and 2 uL of water to the previous set ups (ligated RNA) ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μg of input RNAs and 2.5 μg of RPF RNAs were treated with Ribo-Zero reaction (MRZH11124C, Epicentre) kit by following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... with ribosomal depletion using Human/Mouse/Rat Ribo-Zero kit (Epicentre/Illumina). All samples were sequenced using a 100 base-pair (bp ...
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bioRxiv - Microbiology 2020Quote: ... coli Stellar (Lucigen). PFF1 was isolated from spinach ...