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Citations for Lucigen :
2201 - 2250 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... The DNA was then reacted with EZ-Tn5 transposase (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... was used in 20 µL PCR reactions consisting of 10µL of 2X EconoTaq Master mix (Lucigen), 1uL of each primer (10uM concentration) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Colony PCR was performed as follows: 2X EconoTaq Master mix (Lucigen) was used in 20 µL PCR reactions consisting of 10µL of 2X EconoTaq Master mix (Lucigen) ...
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bioRxiv - Synthetic Biology 2020Quote: ... cloni 10G was obtained from Lucigen. Strains DLF_Z0025 ...
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bioRxiv - Microbiology 2020Quote: Genes were amplified by PCR using the appropriate primers and the amplified DNA cloned in pET28b using NheI/XhoI restriction sites or pETite (ExpressoTM T7 cloning and expression system, Lucigen) generating constructs with either N- or C-terminal His6 tags (Supplementary Table 7) ...
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bioRxiv - Cancer Biology 2020Quote: ... HBV plasmid (positive control) and HBV DNA from inoculum was digested with plasmid-safe DNase (Epicentre, E3101K, Madison, WI). According to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... coli OverExpress C43 (DE3) cells (Lucigen) cells were transformed with the pET-26b vector containing the DNA of GLIC mutants ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli cells (Lucigen), which were plated on LB agar supplemented with 100 µg mL−1 ampicillin ...
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bioRxiv - Molecular Biology 2020Quote: ... Genomic DNA from the whole mosquito was isolated and purified using the MasterPure™ Gram Positive DNA Purification Kit following the manufacturer’s instructions (Epicentre Biotechnologies, Madison, USA). During DNA extraction ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted using the Epicentre MasterPure nucleic acid extraction kit (Epicentre, Madison, WI) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Ribosomal RNA contamination was removed using the Ribo-Zero Magnetic Kit (Bacteria) (Epicentre). Finally ...
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bioRxiv - Microbiology 2020Quote: ... and 23S subunits and spaced 9 bases apart were then annealed at 65 degrees followed by digestion with Hybridase (Lucigen), a thermostable RNAseH ...
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bioRxiv - Microbiology 2020Quote: ... and T-D-F) also collected tongue material by swabbing the tongue dorsum with a sterile Catch-AllTM Specimen Collection Swab (Epicentre Biotechnologies, Madison, WI). Subjects collected dental plaque samples using multiple toothpicks to collect plaque from the gingival margin ...
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bioRxiv - Microbiology 2020Quote: ... PCR-free library preparation (Lucigen) and NextSeq 500 sequencing (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... cDNA libraries were prepared using the ScriptSeqTM Complete Kit (Epicentre, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed in Tris-EDTA (TE) buffer (10 mM Tris-Cl, pH 8.0, 1 mM EDTA) containing lysozyme (Epicentre; R1804M) and 1% SDS ...
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bioRxiv - Microbiology 2020Quote: ... pSMART BAC vector (Lucigen) was digested with Not I ...
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bioRxiv - Microbiology 2020Quote: Escherichia coli TransforMax Epi300 electrocompetent cells (Ref. EC300110, Epicentre, Madison, WI) and Saccharomyces cerevisiae VL6-48N strain [30] were used to propagate the pCC1BAC-His3 containing viral cDNA ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was depleted of ribosomal RNA with the RiboZero gold kit (Epicentre), and enriched mRNA was applied to library preparation according to manufacturer’s protocol (NEBNext Ultra) ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli 10G isa highly competent DH10B derivative [54] originally obtained from Lucigen (60107-1). E ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was depleted of ribosomal RNA with the Ribo-Zero Gold kit (Epicentre). Ribosome-depleted RNA was used to create multiplexed RNA-seq libraries (NEBNext Ultra ...
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bioRxiv - Plant Biology 2020Quote: The LAMP reaction was performed using LavaLAMP™ DNA Master Mix (Lucigen, WI, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... or MasterPure Gram Positive DNA purification kit (Lucigen). Sequencing libraries were prepared using NexteraXT (Illumina) ...
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bioRxiv - Microbiology 2020Quote: The DNA isolation procedure and WGS procedure was performed as follows: DNA was extracted using the MasterPure DNA isolation kit (Lucigen) or MasterPure Gram Positive DNA purification kit (Lucigen) ...
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bioRxiv - Microbiology 2020Quote: ... were prepared by Lucigen (https://www.lucigen.com/) using the pSMART-HCKan cloning vector (Lucigen,WI, USA). Clones were sequenced by Sanger sequencing using the MegaBACE 1000 and 4000 instruments (Symbio Corporation ...
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bioRxiv - Microbiology 2020Quote: ... end-repaired DNA from PoV-01B (1-2 Kb) were prepared by Lucigen (https://www.lucigen.com/) using the pSMART-HCKan cloning vector (Lucigen,WI ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli BL21(DE3) cells (Lucigen).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... circularized using CircLigase II (EpiCentre), annealed to the iCLIP Cut Oligo ...
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bioRxiv - Molecular Biology 2020Quote: ... circularized with Circ Ligase (Lucigen), and PCR amplified ...
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bioRxiv - Molecular Biology 2020Quote: Only cytoplasmic RNA was treated with the Ribo-Zero Magnetic Gold Kit for human/mouse/Rat (Epicentre) to remove ribosomal RNA ...
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bioRxiv - Genetics 2020Quote: ... Intramolecular circularization of the DNA was performed with T4 DNA ligase and residual linear DNA was degraded by a cocktail of exonucleases containing Plasmid-Safe ATP-dependent DNase (Lucigen #E3101K), Lambda exonuclease (#M0262 ...
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bioRxiv - Bioengineering 2020Quote: ... genomic DNA was isolated using QuickExtract DNA Extraction Solution (Epicentre). Two sets of PCR primers were designed ...
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bioRxiv - Bioengineering 2020Quote: ... The cloned libraries were individually amplified by electroporation into Endura ElectroCompetent cells (Lucigen). Using dilution plates for colony counting ...
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bioRxiv - Genetics 2020Quote: ... genomic DNA was isolated from candidate mutants with the MasterPure DNA purification kit (Epicentre) and PCR followed by gel electrophoresis confirmed correct integration of the NAT dominant resistance marker ...
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bioRxiv - Genetics 2020Quote: ... DNA was isolated using the QuickExtract kit (Epicentre, #QE09050) and the target region in the last exon of ACTN2 (Primers ...
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA was isolated from bulk cells transfected with CRISPR/Cas9 plasmids using the epicentre QuickExtract DNA Extraction Solution (Lucigen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: The initial RNA library was transcribed from 1µg of input dsDNA using the AmpliScribe T7-Flash Transcription Kit (Epicentre). 200 ng of DNase treated RNA library was added to 100 nM of Halo-tagged proteins immobilized onto magnetic resin (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... and ribosomal RNA was depleted using the Ribo-Zero Plant rRNA removal kit (Epicentre). chrRNA-seq libraries were constructed from 50 ng of purified RNA using the KAPA Stranded mRNA-seq Kit ...
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bioRxiv - Molecular Biology 2020Quote: ... The remaining DNA was depleted with 2 U of Baseline-ZERO DNase (Epicentre) and ribosomal RNA was depleted using the Ribo-Zero Plant rRNA removal kit (Epicentre) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The synthetic template DNA (IDT) was circularized using CircLigase II (Epicentre), treated with Exonuclease I (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... or the AmpliCap High Yield Message Maker (Epicentre) kits.
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bioRxiv - Neuroscience 2020Quote: ... 0.1 μL 40U/μL RNAsin (Lucigen), and 0.6 μL nuclease-free water (Thermo-Fisher ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 2×105 cells by resuspension in 100 μL of QuickExtract solution (Lucigen, #0905T), incubation at 65°C for 6 min ...
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bioRxiv - Immunology 2020Quote: ... Escherichia coli C43(DE3) cells (Lucigen) transformed with pOPTH-1124 were grown in terrific broth medium ...
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bioRxiv - Synthetic Biology 2020Quote: ... the tet-sacB selection/counterselection cassette was amplified using the appropriate oligos supplying ∼50 bp flanking homology sequences using Econotaq (Lucigen Middleton, WI) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... pSMART-HC-Kan (Lucigen, WI), pTWIST-Chlor-Medium Copy (Twist Biosciences San Francisco ...
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bioRxiv - Biochemistry 2020Quote: ... coli PAP (Lucigen). The PAP reaction mixture (5–10 μL ...
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bioRxiv - Molecular Biology 2020Quote: ... and processing with a RiboZero rRNA removal kit (Epicentre/Illumina) to deplete rRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were treated with Terminator 5′ phosphate-dependent exonuclease (Epicentre) to remove RNAs with 5′ monophosphate (5′ P ...
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bioRxiv - Molecular Biology 2020Quote: ... Tobacco acid pyrophosphatase (TAP, Epicentre) was added to convert 5′ PPP or capped RNAs to 5′ P RNAs ...