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Citations for Lucigen :
1901 - 1950 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... PAP dedicated buffer and PAP (Lucigen) from 0.05 to 0.2 U/μL reaction ...
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bioRxiv - Genetics 2020Quote: Ribosomal RNA-depleted RNA-seq libraries were prepared from 200-500 ng of total RNA using the low input ScriptSeq Complete Gold Kit (Epicentre). Libraries were sequenced on an Illumina NextSeq 500 with single-end 75 bp reads.
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bioRxiv - Cell Biology 2020Quote: ... ruptured cells were treated with 1 U μl−1 OmniCleave endonuclease (Lucigen) for 15 min at 37°C to release the DNA-bound protein pool ...
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bioRxiv - Immunology 2020Quote: ... DNA was extracted 48 hrs after electroporation using QuickExtract DNA Extraction Solution (Epicentre). Genomic DNA was extracted in the same way from NK cells for off-target studies comparing WT and HiFi Alt-R Cas9 RNP complexes with editing mediated by optimized delivery conditions ...
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bioRxiv - Synthetic Biology 2020Quote: ... 400ng of total RNA was used to produce cDNA using MMLV High Performance Reverse Transcriptase (Lucigen RT80125K). TaqMan qPCR was performed using PrimeTime® Gene Expression Master Mix IDT ...
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bioRxiv - Synthetic Biology 2020Quote: ... and T5 Exonuclease (Epicentre) in 5X isothermal buffer (Supplementary Table 6) ...
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bioRxiv - Immunology 2020Quote: ... coli (Epicentre), while sMVA F1 was cloned and maintained in DH10B E ...
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bioRxiv - Biochemistry 2020Quote: buffer supplied from Epicentre in the AmpliScribe T7 High Yield Transcription Kit.
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bioRxiv - Biochemistry 2020Quote: ... The AmpliScribe™ T7 High Yield Transcription Kit was purchased from Epicentre and the HiScribe T7 In Vitro Transcription Kit was obtained from New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... Nematodes were washed individually from each mapping line in approximate equal pellet sizes prior to pooling and genomic DNA extraction using the MasterPure Kit (Epicentre). We sequenced the whole genomes of tu391 and csu60 ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli strains DLF_R002 and DLF_R003 were constructed as previously described.10 Reporter plasmids were constructed in the pSMART-HC-Kan (Lucigen, WI) backbone ...
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bioRxiv - Genomics 2020Quote: The genomic DNA of collected cells was extracted using QuickExtract DNA Extraction Solution (Epicentre). Genomic PCR reaction was performed using 100 ng genomic DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... The ligation product was treated with Plasmid-safe nuclease (10,000 units/ml, Epicentre, E3101K) and transformed into SURE2 cells ...
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bioRxiv - Bioengineering 2020Quote: ... The products of the Gibson reactions were combined and electroporated into Endura cells (Lucigen) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: Golden gate assembly reactions were transformed into chemically competent Escherichia coli prepared from strain TG1 (Lucigen). Transformed cells were selected on Lysogeny Broth containing the antibiotics chloramphenicol ...
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bioRxiv - Genomics 2020Quote: ... Pellets were resuspended in 2 µl water and subjected to electroporation into Endura competent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... TOPO plasmids were electroporated into Endura competent cells (Lucigen) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... followed by resuspension in Quick Extract (Lucigen). Samples were vortexed at top speed for 15 seconds ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μl Plasmid-Safe Enzyme (Lucigen), and 6 μl 10mM ATP (New England Biolabs) ...
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bioRxiv - Cancer Biology 2020Quote: ... the circularization mixture was combined with 6 μl 10X Plasmid-Safe Buffer (Lucigen), 2 μl Plasmid-Safe Enzyme (Lucigen) ...
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bioRxiv - Cancer Biology 2020Quote: ... The linear DNA was then removed using Plasmid-Safe DNase (Lucigen). Briefly ...
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bioRxiv - Synthetic Biology 2020Quote: ... cloni® cells (Lucigen) and plated on 37 large agar plates with Kanamycin antibiotics in order to conserve library complexity ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli “ClearColi” BL21(DE3) (Lucigen), which harbors a modified LPS that does not trigger endotoxic response ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was purified from pelleted cells using a MasterPure Yeast RNA extraction kit (Lucigen, MPY03100), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The ‘curlcake’ constructs were in vitro transcribed using Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507) with either unmodified rNTPs (UNM) ...
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bioRxiv - Molecular Biology 2020Quote: ... subtilis lysine riboswitch and Tetrahymena ribozyme were also produced by in vitro transcription using Ampliscribe™ T7-Flash™ Transcription Kit (Lucigen-ASF3507). All constructs were 5’ capped using vaccinia capping enzyme (NEB-M2080S ...
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bioRxiv - Biochemistry 2020Quote: ... ratio overnight at 16 °C followed by purification and then electroporation into phage-display competent TG-1 cells (#60502-1, Lucigen). The library was plated onto 2xYT agar plates containing 100 µg/mL carbenicillin and 2% glucose at 37 °C overnight ...
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bioRxiv - Genomics 2020Quote: ... Gel-purified cDNAs were then circularized with CircLigase I (Lucigen, Cat: CL4111K) and PCR-amplified with Illumina’s paired-end primers 1.0 and 2.0 ...
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bioRxiv - Genetics 2020Quote: Cells were lysed in plate format in 50µL QuickExtract DNA Extraction Solution (Lucigen QE09050). Crude lysate was then incubated at 65°C for 20 minutes and 95°C for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and resulting double-stranded cDNAs were treated with a combined mixture of RiboShredder RNase Blend (Lucigen) and RNase ...
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bioRxiv - Genetics 2020Quote: ... The genomic DNA extracted using MasterPure Yeast DNA Purification Kit (tebu-bio: now Lucigen) was sequenced using Illumina HiSeq 2000 technology with 100 bp paired-end libraries ...
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bioRxiv - Genetics 2020Quote: ... cloni 10G SUPREME Electrocompetent Cells (Lucigen, #60080-2), in 11 parallel transformations (1 mL each ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted genomic DNA from mouse tails using the QuickExtract DNA extraction kit (Epicentre) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted using the MasterPure™ Yeast DNA Purification Kit (Epicentre MPY80200), with slight modifications compared to the manufacturer’s guidelines as follows ...
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bioRxiv - Genomics 2020Quote: ... coli (Lucigen) and 12μl ligation product ...
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bioRxiv - Genomics 2020Quote: ... First strand cDNA synthesis was done using Episcript™ RNase H-reverse transcriptase (Epicentre) where RNA samples were primed with random hexamers (NEB) ...
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bioRxiv - Genomics 2020Quote: ... separately using EpiScript™ Reverse Transcriptase (EpiCentre Biotechnologies) using both random hexamers (NEB ...
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bioRxiv - Genomics 2020Quote: ... DNA was treated with 10U of Plasmid-safe Dnase (Epicentre® Illumina) for 45 minutes at 37°C following thelatest update of the international working group on cccDNAstandardization (Allweisset al. ...
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bioRxiv - Genomics 2020Quote: ... or homogenized tissues were digested with proteinase K prior to DNA isolation using MasterPure™ DNA Purification Kit (Epicentre, by Illumina, Madison, United States) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... Polyphosphatase (Lucigen) was incubated with smRNA samples before ligation and PCR amplification with primers described by (Goodarzi et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA was then treated with polyphosphatase (Lucigen) before acid phenol chloroform extraction and RNA precipitation ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate was digested with RNase I (Lucigen) before ribosomes were isolated through ultracentrifugation through a sucrose cushion ...
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bioRxiv - Molecular Biology 2020Quote: ... and the RT product was circularized by CircLigase II (Epicentre). A PCR library was amplified and sequenced using Illumina Nextseq 500 at the Rockefeller University Genomics Center.
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bioRxiv - Neuroscience 2020Quote: DNA was lysed with 50 μl QuickExtract™ DNA Extraction Solution (Lucigen). Copy numbers were analyzed by quantitative real-time PCR performed in an ABI Prism 7900 HT Fast Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... 124 nt oligonucleotides (IDT) (see Supplementary Table S7) were circularized using with CircLigase™ single stranded (ss) DNA Ligase (Lucigen) according the manufacturers suggestion ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transferred to tubes containing 3uL of lysis buffer (Epicentre, MessageBOOSTER kit), and stored at - 80° ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA collected from each cell was reverse transcribed and amplified using T7 linear amplification (Epicentre, MessageBOOSTER kit for cell lysate), cleaned with RNA Cleaner & Concentrator-5 columns (Zymo Research) ...
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bioRxiv - Genetics 2020Quote: Total DNA was extracted using a QuickExtract kit (Lucigen). Unless otherwise indicated ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 serial dilution samples and a non-evolved yLL132a ancestor with the MasterPure Yeast DNA Purification Kit (Epicentre, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... 15 μg total RNA were digested with 10 units of RNase R (RNR07250, Epicentre) for 1 hour at 37 °C ...