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Citations for Lucigen :
1801 - 1850 of 2345 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Intra-species transcriptional profiling reveals key regulators of Candida albicans pathogenic traitsbioRxiv - Genomics 2021Quote: ... RNA was harvested from cells using a Masterpure Yeast RNA Purification kit (Epicentre, Madison, WI) and treated with DNaseI (Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... the ligation products were purified by removing the enzymes and transformed into 500 μl of electroporation competent TG1 cells (Lucigen, Middleton, WI) to make the library.
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bioRxiv - Molecular Biology 2021Quote: ... 5ug of RNA from individual tissues were treated with 15 units of RNAse R (Epicentre, Illumina, USA) for 15 minutes at 37 degrees Celsius ...
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bioRxiv - Cancer Biology 2021Quote: ... tissues from secondary GBM (Grade III (n=44), Grade IV (n=23)) and LGG (grade II, n=42) using MasterPure kit (Epicentre). Raw reads from the RNA-seq data were processed using an in-house pipeline that uses STAR for read alignment ...
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bioRxiv - Evolutionary Biology 2021Quote: ... sample 2 was treated with 20 U RNase R (Epicentre/Illumina, Cat. No. RNR07250) for 1 h at 37°C to degrade linear RNAs ...
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bioRxiv - Genetics 2021Quote: ... DNA was extracted from the cells using QuickExtract DNA Extract Solution (Lucigen, QE9050). Clones were genotyped by PCR and Sanger Sequencing (Supplementary Fig ...
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bioRxiv - Microbiology 2021Quote: ... and then ribosomal RNA was removed by a Ribo-Zero Magnetic kit (Epicentre Biotechnologies, USA). RNA quality was checked by NanoDrop (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... ribosomal RNA (rRNA) was removed using the Ribo-Zero Magnetic Kit for bacteria (Epicentre, IL). Metatranscriptome library preparation was performed using the Ion Total RNA-Seq Kit v2 (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... cccDNA was quantified after ExoI + ExoIII endonuclease (Epicentre) digestion of total extracted DNA for 2 hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: Total DNA was extracted using MasterPure™ Complete DNA Purification Kit (Epicentre). Total RNA was extracted using ExtractAll TRI-Reagent (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was directly treated with Baseline-ZERO DNase (2.5 units/50 µL; Epicentre) for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... in combination with 1 µl of RiboGuard RNase Inhibitor (Lucigen) were used instead of the recommended Agencourt RNAclean XP beads to purify samples after enzymatic reactions ...
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bioRxiv - Microbiology 2020Quote: ... rRNAs) was achieved by incubation of the RNA with the Terminator 5’-Phosphate-Dependent Exonuclease (TEX, Lucigen). For this purpose ...
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bioRxiv - Microbiology 2020Quote: ... and 0.5 µl RiboGuard RNase Inhibitor (Lucigen) in a total volume of 20 µl for 60 minutes at 30°C ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl TEX reaction buffer (Lucigen) and 0.5 µl RiboGuard RNase Inhibitor (Lucigen ...
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bioRxiv - Microbiology 2020Quote: ... The samples were chilled on ice for 5 minutes and 175 μl of MPC protein precipitation solution (Lucigen, USA) was added to 300 μl of the lysed sample and vortexed vigorously for 10 seconds ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was isolated from clones following overnight culture using a MasterPure™ Gram Positive DNA Purification Kit (Lucigen Corp., USA). The sequencing library was prepared using the Illumina Nextera kit following published methods (22 ...
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bioRxiv - Microbiology 2020Quote: The MasterPure™ Gram Positive DNA Purification Kit (Lucigen Corp., USA) was used to isolate genomic DNA from S ...
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bioRxiv - Molecular Biology 2020Quote: ... circularized using CircLigase (Epicentre) and PCR amplified (8-12 cycles ...
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bioRxiv - Molecular Biology 2020Quote: ... After treatment with RNase R (Epicentre, Madison, WI, USA) to remove the linear RNAs ...
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bioRxiv - Molecular Biology 2020Quote: ... The cDNA was then circularized using Circligase II (Lucigen, #CL4111K) and then linearized using BamHI ...
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bioRxiv - Genomics 2020Quote: ... Ribosomal RNA (rRNA) in the samples was depleted by Ribo-Zero Magnetic Kit (Plant Leaf) (Epicentre). Then ...
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bioRxiv - Genomics 2020Quote: ... rRNA free samples were used for RNA-seq library preparation using ScriptSeq v2 RNA-Seq Library Preparation Kit (EPicentre). Microsome-polysomal RNA was obtained using a previously described method with some modifications (Li et al. ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA templates were prepared by lysing cells in QuickExtract DNA extraction solution (Lucigen, Cat No. QE0905T). Target regions were amplified by using specific PCR primers (ETV2_F ...
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bioRxiv - Cell Biology 2020Quote: RNA extraction was performed using the MasterPure™ Complete DNA and RNA Purification Kit (Epicentre, MC85200), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... maltophilia transposon insertion mutant library was constructed using an EZ::TN
Tnp transposome kit (Epicentre) as described previously and in accordance with the manufacturer’s protocol (Weisenfeld ... -
bioRxiv - Cell Biology 2020Quote: ... ribosomal RNA was depleted from HCT116 wild-type and KO-CREB1 cells by using a Ribo-Zero rRNA Removal Kit (Epicentre). Then ...
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bioRxiv - Cell Biology 2020Quote: ... 1.5 μL RiboGuard RNAse inhibitor (Lucigen 75935-554), 3 μL 35S-labeled methionine (PerkinElmer NEG709A500UC) ...
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bioRxiv - Cell Biology 2020Quote: ... an oligonucleotide containing the Illumina P7 adaptor sequence was ligated to the 3’ end of the single stranded LAM PCR fragments using Circligase (Epicentre/Illumina, #CL9025K). A P5 adaptor was added by PCR ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was extracted from each sample using the Epicentre MasterPure kit (Lucigen, WI, USA), treated with DNase (2hrs) ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA was isolated from single cell clones using the QuickExtract DNA extraction solution (Epicentre/Lucigen, Middleton, WI) according to manufacturer’s instructions and analyzed for the presence of the wildtype and knock-in Lmna allele by PCR using the GoTaq green master mix (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA was isolated from single cell clones using the QuickExtract DNA extraction solution (Epicentre/Lucigen, Middleton, WI) according to manufacturer’s instructions and analyzed for the presence of the wildtype and knock-in Lmna allele by PCR using the GoTaq green master mix (Promega ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... 10 µg total RNA was diluted in 20 µl of water with 4U RNase R/µg and 2 µl enzyme buffer (Epicentre), then incubated at 37 °C for 3 h ...
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bioRxiv - Cancer Biology 2020Quote: ... and rRNA removed using RiboZero (Epicentre, Wisconsin, USA) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... then 90% of cells were harvested for genotyping using QuickExtract DNA lysis solution (Epicentre #QE0905T), while 10% of cells were re-plated for the next round of cell selection for wells of interest by FACS sorting again or by serial dilution of cells for manual picking of colonies ...
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bioRxiv - Evolutionary Biology 2020Quote: ... dsRNA was made using ribonucleotides from Lucigen.
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bioRxiv - Developmental Biology 2020Quote: Library preparation was adapted from previous protocols(Flynn et al., 2015; Ingolia et al., 2012) and the ARTseq Ribosome Profiling Kit manual (Epicentre, Illumina). In summary ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by the addition of a poly(A) tail using a Poly(A) Polymerase Tailing Kit (Lucigen, PAP5104H). In vitro synthesized capped and poly(A ...
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bioRxiv - Microbiology 2020Quote: ... DNAse-treated RNA (5 µg) was mRNA enriched using a Ribo-Zero Magnetic Kit (Epicentre). After Illumina sequencing the reads were mapped to C ...
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bioRxiv - Developmental Biology 2020Quote: We used 200-250 primordial follicles and 100-150 primary follicles for cDNA synthesis using the Message Booster cDNA synthesis kit (Lucigen, Palo Alto, CA). Direct cDNA and subsequent cRNA syntheses were performed by following the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were then synthesized using the AmpliScribe T7-Flash Transcription Kit (Lucigen) and purified by ethanol precipitation or with RNeasy MinElute Cleanup Kit (QIAGEN) ...
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bioRxiv - Genomics 2020Quote: ... Cells were then lysed with QuickExtract DNA Extraction Solution (Lucigen) and PCR-amplified using primers ...
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bioRxiv - Genomics 2020Quote: ... the ligase-treated DNA was incubated with 2 μl (10 U/μl) of Plasmid-Safe ATP-Dependent DNase (Lucigen; Middleton, WI) in 1x reaction buffer (33 mM Tris-acetate ...
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bioRxiv - Genomics 2020Quote: ... 1 ul Hybridase Thermostable RNase H (Lucigen, H39500) and 1 ul 10 X digestion buffer (500 mM Tris-HCl ...
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bioRxiv - Genomics 2020Quote: ... Bisulfite-converted DNA was used for single-stranded library preparation using the EpiGnome Methyl-Seq kit (Epicentre, EGMK81312) with the described modifications ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted using QuickExtract™ DNA Extraction Solution (Lucigen, USA). Genomic PCR was performed using 100 ng genomic DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was purified using MasterPure Yeast DNA purification Kit (Epicentre) according to the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.4μL 50mM MnCl2 and 0.3μL of 100U/μl CicrLigase II ssDNA ligase (Epicentre CL9021K) and incubated in a PCR machine at 60°C for 60 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... blunt-end ligation into the pZE21 MCS1 expression vector with constitutive promoter pLteo-1 (Lutz and Bujard, 1997) at the HincII site using the Fast Link ligation kit (Epicentre), and iv ...
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bioRxiv - Molecular Biology 2020Quote: ... ssAAV vector samples were incubated with 40-U exonuclease (Lucigen Cat#E3101K) and 10-U endonuclease (Lucigen Cat#DB0715K ...