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Citations for Thermo Fisher :
9951 - 10000 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and cultured in 75 cm2 flasks in a humidified incubator at 37°C under 95% O2 5% CO2 in Eagles minimum essential medium (Gibco) supplemented with 5% fetal bovine serum/5% horse serum (Atlanta Biologicals) ...
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bioRxiv - Neuroscience 2022Quote: ... For in vivo labelling of proliferating SOX10+ cells 5-Ethynyl-2’-deoxyuridine (EdU, 0.3 mg/ml, Thermo Fisher Scientific, E10187) was administered intraperitoneally to pregnant females two hours prior to harvesting of embryos ...
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bioRxiv - Neuroscience 2022Quote: ... followed by mechanical and enzymatic dissociation by pipetting every 5 min in protease solution (0.25% trypsin (Life Technologies, 15090-046), 1 mM EDTA ...
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bioRxiv - Molecular Biology 2023Quote: ... Northern blot analysis of U1 snRNA was performed using 5’-radiolabeled DNA probes (Sequence is listed in Supplemental Table 7) in ultrasensitive hybridization Buffer (Thermofisher). The radioactivity signals were analyzed by PhosphorImager (Typhoon FLA 7000).
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bioRxiv - Molecular Biology 2023Quote: ... hiPSC-CMs were metabolically enriched for 5 d with daily feeding with DMEM without glucose or L-glutamine (Invitrogen, F530S) supplemented with 4 mM Sodium L-lactate (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: SF268 (human glioblastoma) cells were cultured at 37 °C with 5% CO2 in RPMI medium supplemented with 10% FBS (Gibco). Vero (African green monkey kidney ...
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bioRxiv - Neuroscience 2023Quote: ... gene was prepared by first plating 4×106 HEK cells in a T-75 and grown in 5% Foetal Bovine Serum (#FFBS-500, Scientifix) in DMEM/F12 (#10565018, Gibco). The following day cells were transfected using Lipofectamine 2000 (#11668027 ...
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bioRxiv - Microbiology 2023Quote: Normal adult donor blood (in EDTA) was diluted 1:5 with RPMI 1640 supplemented with pen/strep and 2mM L-glutamine (Gibco). Cells were incubated at 37°C for 2 hours in the presence of polymyxin B (1.25μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... and mut-Alkbh5 AAV transfected adult DRG neurons or the L4-5 DRGs at varying times after SNC using TRIzol reagent (Invitrogen). The Seq-StarTM poly (A ...
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bioRxiv - Physiology 2022Quote: ... the membrane was blocked for 45 minutes in blocking buffer (5% w/v bovine serum albumin in tris-buffered saline with 0.1% v/v Tween-20; Fisher Thermo Scientific) and incubated with a custom polyclonal primary antibody against coral soluble adenylyl cyclase (sAC ...
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bioRxiv - Evolutionary Biology 2022Quote: ... iPSCs were split with EDTA at 1:5 ratios in culture dishes coated with matrigel and culture in N2B27 medium (comprised of DMEM/F12 medium (Invitrogen) supplemented with 1% MEM-nonessential amino acids (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... ATCC) were maintained at 37□°C with 5% CO2 in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Gibco, CA, USA) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Genetics 2022Quote: ... β-catenin-Luc plus 5 ng pRL-TK-Renilla were transfected into the cells with Lipofectamine 3000 (Invitrogen, L3000-015) in 96-well plates ...
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bioRxiv - Neuroscience 2023Quote: ... To analyze the proliferative capacity of glial cells we injected 5-Ethinyl-2′-deoxyuridine (EdU, 0.05 mg/g, Thermofisher #E10187) intraperitoneally and animals were sacrificed 1hr after injection.
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bioRxiv - Cancer Biology 2024Quote: ... K562 and HS-5 cell lines were enumerated on the Countess II FL Automated Cell Counter (ThermoFisher, Waltham, MA, USA). The cell lines were tested for mycoplasma contamination using a PCR-based approach.
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bioRxiv - Cancer Biology 2024Quote: ... and washed again with PBS (4 x 5 min) before the slides were mounted using Prolong Gold Antifade (Thermo Fisher).
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bioRxiv - Cell Biology 2024Quote: ... Non-specific binding was inhibited by incubating for 2 h in blocking buffer (5% BSA or Goat Serum (Invitrogen, #500622) + Triton X-100 (VWR ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa Fluor 488-labeled probe was generated through Nick translation system using the Chromatide Alexa Fluor 488-5-dUTP kit (Invitrogen). Labeled probe was precipitated and denatured at 85°C for 5 min ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... cDNA samples were diluted 5-folds and PCR was performed with StepOne Plus Real-Time PCR System (PE Applied Biosystems). The FastStart Universal SYBR Green Master (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... plates were washed twice with TBST and blocked with 5% BSA + 0.1 mg/ml salmon sperm DNA (ThermoFisher, cat# 15632011), 100ul/well ...
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bioRxiv - Neuroscience 2022Quote: ... 1 ng-5 μg of RNA per reaction was used in SuperScript™ First-Strand synthesis kits (Invitrogen, ID: 11904018). Nucleic acid purity was assessed using a NanoPhotometer® (Implen).
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bioRxiv - Neuroscience 2022Quote: ... 5 mM EDTA and 1% BSA in HBSS) and 2μL of 7-aminoactinomycin D (7-AAD, Thermo Fisher Scientific, A1310) at a concentration of 0.1 mg/mL were added ...
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bioRxiv - Neuroscience 2022Quote: ... and POLDIP2 KO cells were maintained at 37°C and 5% CO2 in a culture medium containing DMEM (Thermo Fisher) supplemented with 10% [v/v] Fetal Bovine Serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were cultured at 37°C in humidified air containing 5% CO2 in DMEM (Biological Industries) supplemented with 10% (v/v) FBS (Gibco), penicillin (100 U/ml) ...
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bioRxiv - Neuroscience 2022Quote: ... 20 three months old transgenic TgN3R182C150 mice were immunized initially either with 25 µg of aggregated NOTCH3 EGF1-5 protein (n=11, vaccinated) plus adjuvant (Imject Alum Adjuvant, ThermoFisher) or PBS (n=9 ...
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bioRxiv - Synthetic Biology 2022Quote: ... A 1:10 dilution was performed and 2μl of diluted DNA was amplified by mean of a QuantStudio 5 (Applied Biosystems) thermal cycler using the SYgreen Blue Mix (PCRbio ...
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bioRxiv - Physiology 2022Quote: HEK293-H cells were maintained in suspension at 37°C with 5% CO2 in CD293 medium supplemented with 4 mM GlutaMAX (Invitrogen). For imaging and electrophysiology ...
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bioRxiv - Molecular Biology 2022Quote: ... was grown overnight on blood agar plates (with 5% sheep blood) or in brain heart infusion broth (SA, ThermoFisher Scientific) until OD600=0.4 ...
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bioRxiv - Neuroscience 2022Quote: ... Collected sections were mounted on glass slides, blocked in blocking buffer (5% goat serum, 0.3% Triton X-100 in PBS) (all materials here from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568, 1:200, Invitrogen, A11041 ...
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bioRxiv - Microbiology 2022Quote: ... All cells were cultured at 37 °C under 5% CO2 in DMEM (HyClone) supplemented with 10% FBS (GIBCO, Life Technologies), 100 unit/ml penicillin ...
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bioRxiv - Microbiology 2022Quote: ... All cells were cultured at 37 °C under 5% CO2 in DMEM (HyClone) supplemented with 10% FBS (GIBCO, Life Technologies), 100 unit/ml penicillin ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were separated using 50 cm Acclaim PepMap 100 analytical column (75 μm ID, 3 μm C18) in conjunction with a Pepmap trapping column (100μm × 2 cm, 5 μm C18) (Thermo Scientific) analysed with Orbitrap Fusion Tribrid mass spectrometer (Thermo-Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: HEK293T cell line was purchased from ATCC and incubated at 37°C with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, 11965092), which was supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... and later dyed with 5 μM CFSE (CellTrace™ CFSE Cell Proliferation Kit - For Flow Cytometry, Molecular Probes, Eugene, OR) (1 mL PBS containing CFSE 5 μM per 3 × 107 splenic cells ...
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bioRxiv - Biochemistry 2022Quote: ... medium supplemented with 10 % fetal bovine serum (FBS) and 0.5 mM L-glutamine in humidified atmosphere at 37°C and 5 % CO2 (all from Gibco-Life Technologies).
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bioRxiv - Bioengineering 2022Quote: ... or transwell inserts precoated using 0.15 mg/mL rat collagen I in a humidified 5% CO2 incubator at 37 ± 0.5°C (Isotemp, Thermo Fisher Scientific). The cells were cultured in complete growth medium composed of endothelial cell basal medium (EBM-2 ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions (5 × 105 - 2 × 106 total cells) were washed with PBS and resuspended in 1X Annexin binding buffer (Thermofisher) and washed once ...
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bioRxiv - Systems Biology 2022Quote: ... Cells were cultured under standard conditions (37 °C with 5% CO2) in a series 8000 WJ incubator (Thermo Fisher Scientific) and kept in monolayers up to 90% confluency.
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bioRxiv - Plant Biology 2022Quote: ... We fragmented Enriched samples for 5 mins at 80°C and generated the first-strand cDNA with SuperScript III (Invitrogen) and random primers ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from RNA extracted from ~30 pairs of ovaries or testes dissected from 5-7 days-post-emergence (dpe) Liverpool strain adults using Trizol (Invitrogen). Primers LA1076 then nested with LA1352 (5’ ...
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bioRxiv - Physiology 2022Quote: ... Cells were then incubated in differentiation media consisting of high glucose Dulbecco’s Modification of Eagle Medium (319-005-CL, Wisent, Saint-Jean Baptiste, QC, Canada) supplemented with 5% heat inactivated horse serum (26050088, Gibco™ Fisher Scientific Company ...
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bioRxiv - Developmental Biology 2022Quote: ... Complementary DNA was synthesized from 5 μg total RNA using SuperScript IV First-Strand Synthesis System (Thermo Fisher Scientific, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Monocytes were seeded in T75 cell culture flasks (5 x 106 cells/flask) in α-minimal essential medium (αMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... brucei 427 parasites were grown at 37°C and 5% CO2 in HMI-9 medium supplemented with Penicillin-Streptomycin (Gibco) and 10% Fetal Bovine Serum (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... Eluted cells were spun at 300 g for 5 minutes and resuspended in Gibco Dulbecco’s Modified Eagle Medium with GlutaMAX (DMEM; Thermofisher, 10566016) supplemented with 10% fetal bovine serum serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... 5’-AACGGGAAGCTTGTCATCAA-3’) (Berg et al., 2019) or telomeres (Telo, 5’-UUAGGGUUAGGGUUAGGGUU-3’) (McCaffrey et al., 2017) were transfected using RNAiMAX (Invitrogen). In brief ...
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bioRxiv - Microbiology 2022Quote: ... NIH) host cells were cultured at 37°C with 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Invitrogen, Waltham, MA) containing 10% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... PCR reactions were performed in a 5 uL RNA reaction volume using SYBR™GreenER™ qPCR SuperMix (Applied Biosystems) or SuperScript™ III Platinum™ One-Step qRT-PCR Kit for TaqMan reactions (InvivoGen ...