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Citations for Thermo Fisher :
9751 - 9800 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... was mixed with 300 μL of bacterial overnight culture and 5 mL of molten soft agar (MSM buffer containing 0.4% TopVision Low Melting Point Agarose (ThermoFisher Scientific)) cooled to 32°C ...
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bioRxiv - Neuroscience 2020Quote: ... Retinas were maintained in culture (37°C, 5% CO2) fed by Neurobasal-A media supplemented with 2 mM L-glutamate (GlutaMAX, Gibco) 2 % B27 ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were kept in retrieval solution until reaching room temperature and washed 2 × 5 min with Tris-buffered saline (TBS; ThermoFisher) plus 0.3% (v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Fisher) containing 1x protease inhibitor cocktail III (Calbiochem ...
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bioRxiv - Biochemistry 2021Quote: ... and 300 mM NaCl] was incubated with 20-fold excess (1 mM final concentration) of tetramethylrhodamine-5-maleimide (TMR) (Invitrogen) at room temperature in the dark for 2–4 h ...
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bioRxiv - Microbiology 2021Quote: ... were seeded into one well of a six-well plate and were transfected with 600 ng of plasmid with 5 μL of Lipofectamine 2000 (Invitrogen) in 200 μL of OptiMEM (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5×104 parental control or transporter containing cells were incubated with 0.25μM Calcein-AM (C3100MP ThermoFisher Scientific, Walthman, MA, USA) in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1 M MgCl2, 0.1M Tris HCL, 1% Tx-100, 1x sBlock, 1% sodium deoxycholate, 5 units/mL DNAse I – Thermo Scientific #89836 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5 ng of the Renilla Luciferase internal control plasmid (pRT-TK) were co-transfected by the Lipofectamine 2000 Reagent (Invitrogen). Forty-eight hours after plasmid transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Cas9/sgRNA RNP complex was assembled just prior to injection and after 5 min incubation at room temperature 200 pg of Cas9 protein (Invitrogen) and 100 pg of sgRNA were co-injected into one-cell stage Tg(fli1ep:Lifeact-EGFP)zf495;Tg(kdr-l:ras-mCherry)s916 embryo ...
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bioRxiv - Biochemistry 2021Quote: The input (total Nav1.5 expression) and biotinylated (cell surface-expressed Nav1.5) samples were separated on NuPAGE 4–12% Bis-Tris gels (Invitrogen, ThermoFisher) and transferred to nitrocellulose membrane (iBlot transfer system ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were dried for 20 min at room temperature then resuspended in 5 μL of 1 M Tris and 10 μL of protein loading buffer (2x NuPAGE LDS sample buffer (Invitrogen), 715 mM 2-mercaptoethanol) ...
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bioRxiv - Biochemistry 2021Quote: 24.5 μL of 5 μM solution of every protein was prepared in 96-well plate MicroAmp Optical plate (Applied biosystems), and incubated at room temp ...
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bioRxiv - Immunology 2021Quote: Thymocytes and LN T cells were cultured at 37°C with 5% CO2 in RPMI-1640 (Gibco, Invitrogen Corporation, CA) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: Thymocytes and LN T cells were cultured at 37°C with 5% CO2 in RPMI-1640 (Gibco, Invitrogen Corporation, CA) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... and human phosphatidylinositol 4-phosphate 5-kinase type-1 beta (hPIP5KB; Uniprot #O14986) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). The gene encoding yeast Mss4 (Uniprot #P38994 ...
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bioRxiv - Microbiology 2020Quote: ... and A549 (ATCC® CCL-18) (verified mycoplasma free) were grown at 37°C with 5% CO2 in Dulbecco’s Modified Eagle’s Medium (Gibco, Dublin, Ireland) supplemented with 10 % fetal bovine serum and Penicillin-Streptomycin solution (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Then, 500 μL of ice-cold wash buffer2 (1x PBS, 5% trehalose, 0.5 mg/ml ULTRAPURE BSA (Thermo fisher, AM2616)) was added ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were incubated in DAPI (used at 2 μg/ml in PBS) for 5 min to counterstain nuclei and mounted in Prolong Gold antifade reagent (Invitrogen). Proprietary (Advanced Cell Diagnostics ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5 µg plasmid DNA and 5 µl TransIT-2020 reagent were added into 250 µl of Opti-MEMI Reduced-Serum Medium (Gibco). After adding the mixture into each well ...
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bioRxiv - Cancer Biology 2020Quote: ... Human primary AML cell were grown at 37°C with 5% CO2 in Iscove’s Modified Dulbecco’s Media with Glutamax (IMDM, Gibco, Life Technologies) supplemented with 20% fetal calf serum (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected pregnant mice intraperitoneally with 100 μL of 5-ethynyl-2’-deoxyuridine (EdU, Click-iT EdU Alexa Fluor 647 Imaging Kit, Invitrogen) at 1mg/mL ...
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bioRxiv - Systems Biology 2021Quote: ... Native protein complexes from platelets were obtained by incubating the washed pallet with Pierce IP lysis buffer (25 mM Tris•HCl pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol) (Thermo Scientific) containing protease and phosphatase inhibitors at 4 °C for 5 minutes ...
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bioRxiv - Genomics 2021Quote: ... 10 µg of antibody (3 µg for H3K27ac) was added to 5 µg of sonicated chromatin along with Dynabeads Protein A magnetic beads (Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Immunology 2021Quote: ... were added to the cells and incubated in the dark for 5-10 min prior reading on a Varioskan LUX plate reader (ThermoFisher). Data was processed using GraphPad Prism v9.0.
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bioRxiv - Developmental Biology 2021Quote: ... Samples were transferred to a PepMap 100 C18 trap column (100 µm x 2 cm, 5 µM particles, Thermo Scientific) at a flow rate of 5 µl/min using mobile phase A (0.1% formic acid and 1% acetonitrile in water) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were washed in PBS (2×10 min at 1500 rpm) and RPMI 1640 + 10% fetal bovine serum (FBS) (5 min at 1500 rpm) (all products from ThermoFisher). Monocytes were isolated with anti-CD14 microbeads (Miltenyi ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293T cells were cultured at 37°C in 5% CO2 in DMEM (Biological Industries) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin (Biological Industries) ...
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bioRxiv - Neuroscience 2021Quote: ... A small incision was rapidly made over the cerebellum and .5 to 1µL fluorescent dye (Vybrant DiI cell labeling solution, Thermo Fisher) was injected at the midline of lobules IV/V of the cerebellar cortex using a glass pipette and a pressure pump (Picospritzer III ...
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bioRxiv - Neuroscience 2021Quote: ... pH 6.8) for 5 min at 95° C and loaded to NuPAGE 4-12% Bis-Tris Protein polyacrylamide gels (Invitrogen, #NP0323BOX). After electrophoresis ...
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bioRxiv - Genomics 2022Quote: ... THP-1 cells were separately transduced with lentivirus expressing sgRNA targeting each candidate gene and selected with 5 μg mL-1 Puromycin (A1113802, Gibco) for 7 days ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were centrifuged for 3 mins at 300 x g and resuspended in 5 mL of 5x TrypLE (Cat.No. A1217701; Thermo Fisher) in L15 and incubated on an orbital rotator at room temperature for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and then diluted to 0.5-2 mg/ml in the hybridization solution with 5% dextran sulfate (Cat. No. S4030; Thermo Fisher). Fluorescence RNA in situ hybridization procedure was performed following Thisse and Thisse (2008 ...
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bioRxiv - Immunology 2021Quote: ... 4 million cells per sample were stimulated ex vivo for 3 hours at 5% CO2 at 37°C in Minimum Essential Media (Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2021Quote: ... The membranes were blocked with 5% fat-free milk and thereafter probed with primary antibodies 6xHis Tag Monoclonal Antibody (Invitrogen) followed by incubation with horseradish peroxidase-labeled secondary antibody ...
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bioRxiv - Pathology 2021Quote: ... pellets were resuspended in 50μl of 5% SDS containing phosphate buffered saline and cytoplasmic fractions were mixed 1:1 with 10% SDS solution (Thermofisher) to give a final 5% SDS concentration ...
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β-importins Tnpo-SR and Cadmus and the small GTPase Ran promote ovarian cyst formation in DrosophilabioRxiv - Developmental Biology 2021Quote: ... dissected ovaries were incubated for one hour at room temperature in Grace's media containing 10 μM 5-ethynyl-2′-deoxyuridine (EdU; Life Technologies). The following primary antibodies were diluted in blocking solution and used overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Liver was perfused by pumping (4 ml/min for 5 min) a pre-warmed perfusion buffer (EBSS w/o CaCl2/MgCl2 with 0.5 mM EGTA, Gibco, Life Technologies) through a catheter inserted into the vena cava ...
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bioRxiv - Cancer Biology 2021Quote: ... Murine cell lines were cultured in Dulbeccos’ Modified Eagle Medium/F12 medium supplemented with 5 mg/mL D-glucose (Invitrogen), 0.1 mg/mL soybean trypsin inhibitor type I (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-FU-D131-#6) were labeled by 15 min incubation with 1 μM CFSE (CellTrace CFSE, ThermoFisher Scientific, Ref: C34554). Cells were then resuspended in PBS supplemented with 30% Percoll ...
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bioRxiv - Cancer Biology 2020Quote: ... HGrC1 cells (15 samples including 3 replicates for 5 conditions) were lysed using TRIzol reagent (catalog #15596026, Thermo Fisher Scientific) and RNA was extracted with Direct-zol RNA MiniPrep kit (#R2052 ...
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bioRxiv - Developmental Biology 2021Quote: ... each sample was dragged through wet lens paper with light pressure to remove the paratenon sheath and then stained with dichlorotriazinylaminofluorescein (5-DTAF, Invitrogen) by incubating in a 5 µg/ml solution of 5-DTAF and 0.1 M sodium bicarbonate buffer (pH 9.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5 ng of the Renilla Luciferase internal control plasmid (pRT-TK) were co-transfected by the Lipofectamine 2000 Reagent (Invitrogen). Fourty-eight hours after plasmid transfection ...
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bioRxiv - Cell Biology 2021Quote: ... both human primary myogenic progenitors isolated from female donors (n=2-5 per experiment) and commercialised human primary skeletal muscle progenitors (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The Stim coding sequence was cloned by PCR using primers 5’-CAC CAT GCG AAA GAA TAC CAT TTG GAA C-3’ and 5’-TTC CGT GGC AAG CAG CGA AAA GTT C-3’ and ligated into pENTR/D-TOPO (Invitrogen). Site-directed mutagenesis (Stratagene QuikChange XL ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37 °C with 5% CO2 in phenol-free Dulbecco’s modified Eagle’s medium (DMEM) (Thermo-Fisher, Gibco™, 31053028) containing 4.5 g/L glucose and supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... with 10% FBS and 1% pen/strep and MCF10A cells were cultured in DMEM/F-12 supplemented with 5% horse serum (Gibco), 20ng/ml epidermal growth factor ...
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bioRxiv - Cell Biology 2020Quote: ... The production of ROS was assessed by the oxidation of 5-6-chloromethyl-2,7-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) (Invitrogen Corporation). Cells cultured in 96-well plates were incubated with JC1 ...
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bioRxiv - Cell Biology 2021Quote: Whole fly RNA was prepared from mixed sex samples (5-15 flies per sample, flies < 1 week old) with Trizol reagent (Invitrogen) according the manufacturer’s protocol ...