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Citations for Thermo Fisher :
9651 - 9700 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Cells were washed once with CYE and further incubated 5 min with Alexa488- or Alexa561-labeled anti-rabbit secondary antibodies (Thermofisher). Cells were washed four times in CYE and concentrated 5-fold ...
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bioRxiv - Cancer Biology 2019Quote: All cells are grown at 37°C with 5% of CO2 with 100 UI/mL Penicillin and 100 µg/mL Streptomycin (Gibco). A673/TR/shEF (Carrillo et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... as well as human hepatoma Huh7.5 cells28 were maintained at 37 °C with 5% CO2 in Dulbecco’s Modified Medium (DMEM) (Invitrogen, Paisley, UK) containing 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... of SARS-CoV-2 S protein and a C-terminal polyhistidine tag was amplified and inserted into a pFasebac1 vector for expression in High-5 insect cells using the Bac-to-Bac expression system (Invitrogen). The resulting recombinant protein ...
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bioRxiv - Immunology 2019Quote: ... 100µl cell suspension was embedded by centrifugation (5’ at 1230 g at 30°C) in 100µl 2% Low Melting Agarose (LMA, Gibco BRL) in 0.1M cacodylate buffer ...
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bioRxiv - Microbiology 2021Quote: ... The cells were cultured at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Thermo Scientific; 31600-034) that contained 3.5 g/L D-glucose ...
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bioRxiv - Microbiology 2021Quote: ... The cells were left to fix for 1 h at room temperature before transferring 5 μl into 15 μl ProLongTM Gold Antifade mountant (ThermoFisher). For cell membrane staining ...
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bioRxiv - Molecular Biology 2021Quote: ... all proteolytic digests were acidified by addition of 10% formic acid to pH 2 and directly analyzed by LC-ESI-MS(/MS) using an a capillary BioBasic C18 reversed-phase column (BioBasic-18, 150 x 0.32 mm, 5 μm, Thermo Scientific), installed in an Ultimate U3000 HPLC system (Dionex) ...
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bioRxiv - Biochemistry 2021Quote: ... cells were cultured at 37 °C in humidified 95 % air with 5 % CO2 in Dulbecco’s modified essential medium (DMEM) (Gibco, Life Technologies) containing glucose (4.5 g/L) ...
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bioRxiv - Physiology 2021Quote: ASM cells and arteries were respectively loaded with 5 μM and 10 μM Fluo-4-AM (Invitrogen, Thermo Fisher Scientific) in a buffer solution containing 140 mM NaCl ...
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bioRxiv - Physiology 2021Quote: ASM cells and arteries were respectively loaded with 5 μM and 10 μM Fluo-4-AM (Invitrogen, Thermo Fisher Scientific) in a buffer solution containing 140 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HEK293T/17 cells were cultured at 37°C and 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, 10567-014) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO] ...
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bioRxiv - Developmental Biology 2021Quote: ... all cells were cultured at 37°C, 5% CO2 in growth medium (DMEM, 10% Embryonic Stem Cell qualified FBS (Gibco), NEAA ...
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bioRxiv - Cancer Biology 2021Quote: ... Suspension was centrifuged at 200g for 5 minute and cells were resuspended in 1ml of full medium (DMEM + 10% FBS; Gibco). Cells were either plated in 2D on cell culture treated flat bottom 96-well plates (Falcon ...
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bioRxiv - Molecular Biology 2021Quote: To label HELB with QDs we incubated a 1:5 molar ratio solution of biotinylated HELB and streptavidin-coated QDs 525 (Q10143, Invitrogen) for 30 min on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... resuspended in 5 mL PBS and crosslinked at room temperature with 1.5 mM ethylene glycol bis-succinimidyl succinate (EGS, Thermo Scientific). After 30 min of incubation ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized using the env V1/V3 Primer ID primer (HXB2 positions 6585-7208): 5’-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNCAGTCCATTTTGCT CTACTAATGTTACAATGTGC-3’ and SuperScript III Reverse Transcriptase (Invitrogen). The final cDNA reaction contained the following ...
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bioRxiv - Microbiology 2020Quote: ... one or two guanines were added to the 5’ end of the guide sequence within the primer to ensure the format “5’-GG(N18-20)-3’” in order to facilitate in vitro transcription with MEGAscript T7 in vitro transcription kit (Ambion). Transcribed sgRNAs were purified with MEGAclear kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed four times with TBS-T for 5 minutes prior to secondary staining with HRP-conjugated goat anti-mouse and goat anti-rabbit antibodies (Invitrogen). For detection ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were initially trapped in C18 PepMap100 pre-column (300 µm inner diameter x 5 mm, 100A, Thermo Fisher Scientific) in Solvent A (Formic acid 0.1% (v/v) ...
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bioRxiv - Microbiology 2021Quote: Harvested cells were concentrated by centrifugation at 10,000 rpm for 1 min and immediately loaded on a 3% w/v agarose (in water) pad on a microscope slide supplemented with 5 µg/ml membrane dye Nile Red (Invitrogen). The agarose pad was made following the protocol described previously (15) ...
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bioRxiv - Microbiology 2021Quote: ... boiled at 100°C for 5 min and then separated on NuPAGE Novex 4-12% Bis-Tris midi gels (Invitrogen) using NuPAGE MES SDS Running buffer (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS and incubated for 1 h at 37°C and 5% CO2 with Opti-MEM (Gibco) containing the indicated inhibitors ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed once with PBS and incubated for 2 h at 37°C and 5% CO2 with Opti-MEM (Gibco) containing 5 or 2.5 μM remdesivir alone or in combination with 10 μM ramelteon (PubChem ID 208902) ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei are stained with DAPI for 5 min before mounting with ProLong™ Gold Antifade Mountant (Thermo Fisher Scientific, P36930).
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bioRxiv - Developmental Biology 2020Quote: Neural retinae were dissected from adult zebrafish dark adapted for 12 hours between 5:30 PM and 6:30 PM and stored in RNAlater (Ambion) at 4°C until extraction ...
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bioRxiv - Biochemistry 2020Quote: ... and an Acclaim™ Pepmap™ 100 C18 analytical column (length 15 cm, 5 μm particle size, Thermo Scientific, USA). 2 μL of the bacteriocin sample was loaded onto the trap column at 3% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... biotinylated amyloid peptides spanning the amyloid beta sequence (−5 to 45) were pre-incubated with NeutrAvidin (ThermoFisher Scientific, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: HeLa and RPE1 (ATCC) cells were incubated at 37°C in 5% CO2 in high glucose Dulbecco’s modified eagle media (DMEM) (Life Technologies), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2019Quote: ... with 10% fetal calf serum (FCS) at 37°C in an atmosphere with 5% CO2 and transfected with lipofectamine (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were washed in PBS (2×10 min at 1500 rpm) and RPMI 1640 + 10% fetal bovine serum (FBS) (5 min at 1500 rpm) (all products from ThermoFisher). Monocytes were isolated with anti-CD14 microbeads (Miltenyi ...
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bioRxiv - Genomics 2019Quote: ... Cells were resuspended in 1 ml IP lysis buffer [25 mM Tris-HCl pH 7.5 / 150 mM NaCl / 1% NP-40 / 1 mM EDTA / 5% glycerol / Halt protease inhibitor cocktail (Thermo scientific) / Halt phosphatase inhibitor cocktail (Thermo scientific)] and incubated on ice for 1 h ...
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bioRxiv - Plant Biology 2020Quote: Regions screened for transcription factor binding via Yeast One-Hybrid were first inserted into pENTR 5’TOPO TA entry vector (Thermofisher) and subsequently placed into the pMW2 and pMW3 destination vectors containing HIS3 and LACZ marker genes respectively (Deplancke et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... Input for each sample consisted of 500 ng of RNA and 5 μL of 1:500 diluted ERCC spike-in mix 2 (Ambion). Libraries were amplified for 11 cycles during the final amplification step ...
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bioRxiv - Synthetic Biology 2020Quote: ... The membrane was blocked with 5% skim milk in TBST and probed with HRP-conjugated anti-flag mouse monoclonal antibody (Thermofisher) diluted at 1:10000 in the blocking buffer ...
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bioRxiv - Bioengineering 2020Quote: ... the cells were stained for 5 minutes at room temperature with LIVE/DEAD Fixable Violet Dead Stain Kit (ThermoFisher L34864). Subsequently ...
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bioRxiv - Synthetic Biology 2020Quote: ... in 50 ml 1X TAE prepared in Milli-Q® water + 5 μl SYBR™ Safe DNA Gel Stain (Invitrogen™ ...
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bioRxiv - Cell Biology 2020Quote: ... The final concentration of the TMT labeled digest/peptide mixture was 0.33 μg/μl and 5 μl of this mixture was loaded onto a C18-reversed phase column (Thermo Scientific, Acclaim PepMap C18 column ...
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bioRxiv - Immunology 2020Quote: ... were added to 7.5□μL of a master mix containing 5□μL of Power SYBR green mix (4367659, Applied Biosystems) and 2.5□μL of nuclease-free water with nCoV_IP2 primers (nCoV_IP2-12669Fw ...
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... and mAb 18B7 (10 µg/ml) for 30 minutes followed by incubation with Alexa-Fluor 546 anti-mouse IgG1 (5 µg/ml; Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Biochemistry 2021Quote: To each well in the plate was added 25 ng of pMIR-B3GLCT plasmid in 5 μl Opti-MEM (Gibco) and 0.11 μl lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were maintained at 37°C in 5% CO2 in serum-free Neurobasal plus medium containing supplement B27 plus (Invitrogen) for up to 3 weeks ...
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bioRxiv - Neuroscience 2020Quote: ... 5% DTT) for 10 min at 70 °C before being loaded on a 4-12% Bis-tris gel (ThermoFisher NP0322BOX) at 10 µg/lane ...
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bioRxiv - Neuroscience 2020Quote: ... at 37°C and 5% CO2 in mitotically arrested Mouse Embryonic Fibroblasts (MEFs) conditioned media (DMEM/F12 (Life Technologies, #11330057), 20% Knock-out Serum Replacement (KSR ...
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bioRxiv - Developmental Biology 2021Quote: ... and the solution was denatured at 95°C for 5 min and run on a ABI3130XL Genetic Analyzer (Applied Biosystems), using GeneMapper software (Life Technologies).
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed 3x 5 minutes in PBST and then incubated for 2 hours at room temperature with Alexa-Fluor secondary antibodies (ThermoFisher) (all 1:1000 in PBST + 5% BSA + 0.02% azide) ...
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bioRxiv - Plant Biology 2020Quote: ... 60-mer DNA oligonucletoides were 5’ end-labeled using [γ-32P]-ATP and PNK (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were pre-treated with 5-ethynyl uridine (EU) for 30 min prior to nuclei fractionation using Sigma kit (#NUC101) followed by Invitrogen Click-iT™ Nascent RNA Capture Kit protocol for nascent transcript extraction (#C10365) ...
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bioRxiv - Genomics 2021Quote: ... American Type Culture Collection (ATCC)] were cultured at 37°C and 5% CO2-concentration in DMEM supplemented with 10% fetal bovine serum (Gibco Invitrogen), 100 units of potassium penicillin and 100 μg of streptomycin sulfate per 1 ml (Lonza) ...