Labshake search
Citations for Thermo Fisher :
9601 - 9650 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2019Quote: ... Forward and reverse primers were added to a final total primer concentration of 0.1 μM in addition to 5 μL of Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher). Nuclease-free water was added for a final reaction volume of 10 μL ...
-
bioRxiv - Developmental Biology 2020Quote: ... for 3 to 5 min at 37°C and resuspended in differentiation medium N2B27 (Advanced DMEM F12, Neurobasal vol:vol (Life Technologies)) ...
-
bioRxiv - Biophysics 2020Quote: ... cells grown in glass-bottom dishes in FBS-containing medium were stained with 5 μM laurdan (Molecular Probes, Eugene, OR) for 5 min ...
-
bioRxiv - Cell Biology 2019Quote: Cells expressing G12V Rac1A under a tetracycline-inducible promoter along with GFP-WASP or RFP-tagged active Rac marker were resuspended in LoFlo and seeded to a final concentration of 5×105 cells/ml on a borosilicate glass 8 well chamber (ThermoFisher) or on a 35 mm glass bottom dish (MatTek) ...
-
bioRxiv - Microbiology 2019Quote: ... Blots were blocked and proved with 1° antibody in 5% milk (vol/vol) in 50 mM Tris-buffered saline with 0.05% (vol/vol) Tween-20 (Fisher Scientific). Monoclonal antibodies against Mycobacterium tuberculosis GlcB (Gene Rv1837c) ...
-
bioRxiv - Bioengineering 2019Quote: ... The pre-PCR amplification reaction was performed in a 5 µl reaction mixture containing 2.5 µl TaqMan PreAmp Master Mix 2X (Applied Biosystems), 1.25 µl of 96-pooled LNA™ assay mix (0.25X ...
-
bioRxiv - Microbiology 2019Quote: ... CD14-positive monocytes were seeded into 12 well plates at 5 × 105 cells/well and differentiated into macrophages by culture in complete RPMI 1640 medium (Gibco) supplemented with 10% heat-inactivated human AB+ serum ...
-
bioRxiv - Neuroscience 2019Quote: ... and then transfected with 2.5 µg of donor DNA and 1.25 µg of each targeting construct (Supplemental Table 3) using Lipofectamine Stem (Invitrogen STEM00003) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... Transferred RNA was UV cross-linked and hybridized overnight with 100 ng/mL of 5’-biotinylated probes (Table S2) in ULTRAhyb hybridization buffer (Ambion) at 42 °C as described previously (27 ...
-
bioRxiv - Microbiology 2019Quote: ... Beads were then washed with ice-cold lysis buffer 3 times for 5 minutes and proteins were recovered by boiling in denaturing loading buffer (Invitrogen).
-
bioRxiv - Neuroscience 2020Quote: ... Briefly, cDNA was derived from 5 week-old C57BL/6J retina (Jackson, 000664) following RNA extraction (RNAqueous Kit, Ambion, AM1912) and reverse transcription with Superscript III (ThermoFisher ...
-
bioRxiv - Genomics 2019Quote: ... and slides were rinsed in PBS for 5 minutes three times before a cover slip was applied using ProLong Gold Antifade Mountant (P10144, Invitrogen). 40x images representative of the sample were obtained by a blinded investigator using a Nikon Eclipse 80i with Ds-Ri2 camera and NIS Elements V4.3 software (Nikon Instruments ...
-
bioRxiv - Cancer Biology 2019Quote: ... VA) and cultured at 37°C in a humidified 5% CO2 atmosphere in DMEM medium (Gibco; Thermo Fisher Scientific, Inc.) with 10% fetal calf serum (Gibco ...
-
bioRxiv - Cancer Biology 2019Quote: ... VA) and cultured at 37°C in a humidified 5% CO2 atmosphere in DMEM medium (Gibco; Thermo Fisher Scientific, Inc.) with 10% fetal calf serum (Gibco ...
-
bioRxiv - Plant Biology 2019Quote: ... and appropriate buffer containing deoxynucleoside triphosphates (dNTPs) and MgCl2 in a final concentration of 5 mM (1×; Thermo Fisher Scientific), was incubated at 25°C for 10 min and at 37°C for 30 min ...
-
bioRxiv - Microbiology 2020Quote: ... 100 ng of extracted vRNAs were reverse transcribed using a Uni-12 primer (5’-AGCRAAAGCAGG-3’) and Superscript III reverse transcriptase (Invitrogen). Quantification was performed by qPCR on a Rotor-Gene Q 2plex System (Qiagen ...
-
bioRxiv - Microbiology 2019Quote: ... at a 1:10000 dilution in 5% skimmed milk-PBST and 1:20000 secondary HRP Rabbit-anti-mouse antibody (Invitrogen) in 5% skimmed milk-PBST16.
-
bioRxiv - Developmental Biology 2019Quote: HEK293 cells were passaged in HyClone DMEM (GE) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco) at 37°C and 5% CO2 under humidified conditions ...
-
bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2019Quote: ... Cells were pelleted by centrifugation at 300RCF for 5 minutes and re-suspended at a density of 6,000 cells/µl in warm Neurobasal-A (Gibco #10888022) + 1X B27 supplement (Gibco #17504044 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were then permeabilized and blocked for 5 min in DPBS containing 0.2% saponin and 10% FBS (Thermo Fisher Scientific). Cells were stained with primary (1:25-50 ...
-
bioRxiv - Synthetic Biology 2020Quote: Samples were run on 4% agarose (Bio-Lab, Israel) in 1× TBE buffer (Biological industries, Israel) supplemented with 5 mM MgCl2 (Ambion), and stained with 0.3 µg/ml ethidium bromide (Sigma-Aldrich) ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Cells were harvested for flow cytometry using FACS buffer (PBS pH 7.4, 2–5 mM EDTA, 0.1% BSA) or using 0.05% Trypsin-EDTA (Thermo Fisher Scientific #25300120) for 5 min followed by quenching with medium ...
-
bioRxiv - Immunology 2020Quote: ... centrifuged at 300g for 5 min and resuspended in 3 mL of 1X ebioscience Red Blood Cell Lysis Buffer (ThermoFisher) for 5 min at 37°C ...
-
bioRxiv - Neuroscience 2020Quote: ... was added to 7.5 μL of a master mix containing 5 μL of Power SYBR green mix (4367659, Applied Biosystems) and 2.5 μL of nuclease-free water with the nCoV_IP2 primers (nCoV_IP2-12669Fw ...
-
bioRxiv - Neuroscience 2020Quote: ... was added to 7.5 μL of a master mix containing 5 μL of Power SYBR green mix (4367659, Applied Biosystems) and 2.5 μL of nuclease-free water containing predesigned primers (#249900 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 7.5 µL master mix was generated for each well in the following ratios: 5 µL genotyping Taqman mix (ThermoFisher Scientific), 0.5 µL of each the Met and Tfrc copy number probes (Table S5) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Click-iT Alexa Fluor 488 EdU (5-ethynyl-2’-deoxyuridine) imaging kit and Lipofectamine RNAiMAX was purchased from Life Technologies. Foetal Bovine Serum (FBS ...
-
bioRxiv - Immunology 2020Quote: ... for 2 h at 37°C and 5% CO2 in the presence of dextran-rhodamine (0.5 mg/mL) (Molecular Probes). mAb-untreated Pf sporozoites were used to measure the maximum traversal capacity ...
-
bioRxiv - Bioengineering 2020Quote: ... falciparum parasite culture was maintained in 5% human red blood cells purified from whole blood (Research Blood Components) with complete RPMI medium (Gibco) supplemented with hypoxanthine (Sigma-Aldrich) ...
-
bioRxiv - Bioengineering 2019Quote: ... or human organoid basal culture media consisting of conditioned media produced using stably transfected L cells (Wnt 50%; R-spondin 10%; Noggin 5%) and the following growth factors: B12 1X (Invitrogen), Nicotinamide 10mM (Sigma-Aldrich) ...
-
bioRxiv - Physiology 2019Quote: ... 100□μl of PBS solution containing 5□mg/ml of 70-kDa Texas Red-conjugated dextran (Thermo Fisher Scientific, USA) was injected intravenously prior to imaging ...
-
bioRxiv - Cell Biology 2019Quote: Soft agar assays were carried out in 6-well plates previously coated with a 5 ml layer of medium containing 40% Dulbecco’s modified Eagle’s medium 2X (Thermo Fisher Scientific), 10% FBS (Gibco ...
-
bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... was added to the cells and incubated in the dark for 5-10 minutes prior to reading on a Varioskan LUX plate reader (ThermoFisher). Measurements were done in duplicate and relative luciferase units (RLU ...
-
bioRxiv - Developmental Biology 2019Quote: ... then washed 2x in PBX for 5 minutes each and placed in a 5µg/ml solution of Hoechst 33342 (Life Technologies) in PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... The loading solution contained a 1:10 mixture of 5 mM Fluo-4 AM in dry DMSO and PowerloadTM concentrate (Invitrogen), which was diluted 100-fold into extracellular Tyrode’s solution (NaCl 140mM ...
-
bioRxiv - Molecular Biology 2020Quote: ... Peptides were trapped at 20 μl/min in loading solvent (2% acetonitrile, 0.05% TFA) on a 5 mm x 300 μm C18 pepmap cartridge pre-column (Thermo Fisher Scientific / Dionex Softron GmbH ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... PCR was conducted using a 5’ fluorescently-labelled primer (6-FAM, PET, NED, or VIC) (Applied Biosystems, Foster City, CA) and an unlabeled reverse primer ...
-
bioRxiv - Bioengineering 2020Quote: ... centrifuged at 300 g for 5 min and seeded at a density of 50,000 cells/cm2 in Dulbecco’s Modified Eagle Medium (Invitrogen, Carlsbad, CA),supplemented with 10% v.v ...
-
bioRxiv - Developmental Biology 2020Quote: ... Slides were then washed with PBS-T (3 × 5 min) and incubated with fluorophore-conjugated secondary antibodies (1:500 in blocking buffer, Invitrogen). Hoechst 33258 (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: HeLa cells were cultured at 37°C and 5% CO2 in DMEM containing 10% [vol/vol] foetal bovine serum (Invitrogen). For passaging ...
-
bioRxiv - Molecular Biology 2021Quote: ... followed by 1 h incubation with 1:1000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse BM-MSCs were first transfected with lentiCas9-Blast vector (Genomeditech) and selected with 5 μg/ml blasticidin S HCl (A1113903, Invitrogen). Then ...
-
bioRxiv - Pathology 2020Quote: ... 5 µL of a 50/50 slurry of streptavidin coated magnetic beads (Dynabeads MyOne C1, Thermo Fisher Scientific, Waltham MA) in 1x-PBS was added to each cRASL reaction and incubated with gentle shaking for 15 min at room temperature ...
-
bioRxiv - Neuroscience 2021Quote: ... Longitudinal imaging of vascular leakage in C57BL/6 mouse was performed for 5 days after injecting 600 μg of 3kDa TRITC-dextran (LIF-D-3308, Invitrogen) and 600 μg of 2MDa FITC-dextran (LIF-D-7137 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were maintained at 37 °C in a humidified 5% CO2 incubator in Dulbecco’s Modified Eagle’s Medium (GIBCO, CA, USA) containing 10% fetal bovine serum (GIBCO ...
-
bioRxiv - Molecular Biology 2020Quote: E14 ES cells were grown at 37°C in 5% CO2 on gelatin-coated plates in media comprising high glucose DMEM (Gibco), 15% foetal bovine serum (Gibco) ...
-
bioRxiv - Cancer Biology 2020Quote: BMDM were isolated from tibia of WT and Thbs4-/- mice as described by others (36) and in our previous publication (5) and cultured in M-CSF (14-8983-62, ThermoFisher)/DMEM/F12 for 5 days ...
-
bioRxiv - Neuroscience 2021Quote: ... tau proteins were measured with Tau-5 antibodies using ELISA according to manufacturer’s instructions (The Human Tau Solid-phase sandwich ELISA, #KHB0041, Thermo Scientific).