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Citations for Thermo Fisher :
9801 - 9850 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: SH-SY5Y cells (Human Neuroblastoma ATCC - CRL-2266) were cultivated in 25 cm2 cell culture bottles at 37 °C under 5 % CO2 in DMEM/F12 (Gibco - Dulbecco’s Modified Eagle Medium - Nutrient Mixture ...
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bioRxiv - Neuroscience 2020Quote: WiBR3 hES cell line and the generated iPS cell lines were maintained in 5% CO2 conditions on irradiated DR4 mouse embryonic fibroblasts (MEF) feeder layers in FGF/KOSR conditions: DMEM-F12 (Gibco; 21331-020 or Biological Industries ...
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bioRxiv - Microbiology 2021Quote: ... the mixture was incubated for 5 minutes at room temperature and then the beads were pelleted on a magnetic stand (Invitrogen) for 2 minutes ...
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bioRxiv - Genetics 2021Quote: Hap1 TP53 cells were cultured in IMDM media and supplemented with 10% Fetal-GRO and 5% penicillin and streptomycin (Gibco) and L-glutamine (Gibco) ...
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bioRxiv - Genomics 2021Quote: ... RNA purity and concentration were determined and cDNA was synthesized using 5 μg RNA and the MMLV reverse transcription kit (Invitrogen). PCR was performed using 20 μl reaction volume contained 1 × PCR Buffer ...
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bioRxiv - Biophysics 2020Quote: ... Roughly 2–5 μL of DNA (~80 ng/mL concentrations) were slowly pipetted into ~50 μL of competent cells (Invitrogen). The cells were left on ice for 20 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... SK-N-FI) and NCI-H23 cells (human NSCLC) were cultivated at 37°C with 5 % CO2 in RPMI 1640 medium (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Cells were precipitated by centrifugation at 1500 rpm for 5 minutes and resuspended in DMEM (0.2% Primocin and 10% chicken serum (Life Technologies)) ...
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bioRxiv - Immunology 2020Quote: Cytokines (IL-5 and IL-13) in supernatant of human and murine ILC2 cell cultures were analyzed with ELISA kits from Invitrogen. For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend ...
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bioRxiv - Microbiology 2019Quote: ... The RNA was precipitated with ice-cold isopropanol and 3M sodium acetate (pH 5) at 4°C before RNA pellets were resuspended in nuclease-free H2O and treated with DNase I (Ambion) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Untranslated regions were cloned using synthetic DNA (Integrated DNA Technologies) or by isolation using 5′ RACE (RLM-RACE kit, Invitrogen). Template was PCR amplified using Phusion polymerase from the plasmids using the following primers ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from groups of 5 HIOs per replicate with a total of 4 replicates per condition using the mirVana miRNA Isolation Kit (ThermoFisher). Cytosolic and mitochondrial ribosomal RNA was removed from samples using the Ribo-Zero Gold Kit according to manufacturer’s instructions (Illumina) ...
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bioRxiv - Microbiology 2020Quote: ... were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 5% fetal bovine serum or 10% horse serum (BT cells) and 100 IU/ml penicillin/streptomycin (Gibco). HeLa PKRkd cells were maintained in medium containing 1 µg/ml puromycin (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... Washed PBMC was then replenished with 5 ml of R10 supplementing with 2,000 U/ml of rh GM-CSF (ThermoFisher Scientific) and 4,000 U/ml of rh IL-4 (R&D Systems ...
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bioRxiv - Microbiology 2020Quote: ... Baby hamster kidney strain 21 (BHK-21, ATCC CCL-10) cells were maintained at 37°C and 5% CO2 in the DMEM medium (Gibco) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2020Quote: ... then resuspended at 2 × 107 cells/mL in growth medium supplemented with 5 mM biotin-phenol (biotin tyramide, Acros Organics). After a one-hour incubation ...
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bioRxiv - Immunology 2021Quote: ... 4 million isolated splenocytes or inguinal lymph node cells were stimulated ex vivo for 3 hours at 5% CO2 at 37°C in Minimum Essential Media (Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: HeLa and MelJuSo cell lines were maintained at 37°C/5% CO2 in Gibco Iscove’s Modified Dulbecco’s Medium (IMDM, Life Technologies-Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... or anti-inflammatory (Mφ2) macrophages with 5 ng/ml of granulocyte-macrophage colony- stimulating factor (GM-CSF; Life Technologies-Invitrogen) or 50 ng/ml macrophage colony- stimulating factor (M-CSF ...
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bioRxiv - Immunology 2021Quote: ... 6.8 µL of 10 µM RNA stock solution and 5 µL of transfection reagent were added in 200 µL Opti-MEM (Invitrogen) to make the transfection mixture ...
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bioRxiv - Microbiology 2021Quote: ... the bacteria were grown overnight at 37°C with 240 RPM agitation in 5 mL of Lennox L broth (LB, Invitrogen) then diluted 1/20 in pre-warmed DMEM 25 mM Hepes (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Coarse particles were allowed to settle out for 5 minutes and supernatant was poured into 250ml polycarbonate Nalgene centrifuge tubes (ThermoFisher). Fine particles were removed by successive centrifugation (pelleting ...
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bioRxiv - Immunology 2020Quote: ... and washed once in droplet media comprising RPMI w/o phenol red with supplemented 5% KnockOut Serum Replacement (both ThermoFisher), 0.5% recombinant human serum albumin (A9986 ...
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bioRxiv - Immunology 2021Quote: ... was added to the cells and incubated in the dark for 5-10 min prior reading on a Varioskan LUX plate reader (ThermoFisher). Measurements were done in duplicate with two independent productions of pseudotyped viruses and RLU values were converted to percentage of neutralization and plotted with a nonlinear regression curve fit in Graph Prism.
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bioRxiv - Immunology 2021Quote: ... Qβ-VLPs were then re-packaged with B-type 1668 CpGs (5″-TCC ATG ACG TTC CTG ATG CT-3″) with phosphorothioate backbone purchased from (InvitroGen). The re-packaging was confirmed by 1% agarose gel stained with SYBR Safe dye for 30min at 90V ...
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bioRxiv - Neuroscience 2020Quote: ... C2C12 cells were allowed to differentiate into myotubes in DMEM supplemented with 5% (v/v) horse serum (Gibco-Life Technologies) and 1% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... C2C12 cells were allowed to differentiate into myotubes in DMEM supplemented with 5% (v/v) horse serum (Gibco-Life Technologies) and 1% (v/v ...
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bioRxiv - Immunology 2020Quote: ... 0.5 mM EDTA; 0.5 mM EGTA; 1% Triton X-100; 0.5 mM PMSF; and Protease Inhibitor Cocktail [Thermo Fisher Scientific]). Protein concentration was measured using a BCA protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... HepG2 or Vero E6 cells were plated in a 12-well plate at 5 x 10E5 cells/well in DMEM medium (Gibco) supplemented with 5% FCS ...
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bioRxiv - Plant Biology 2020Quote: ... radiolabeled probes were prepared from linearized plasmid templates (SpeI or NcoI) having 5’ overhangs from either T7 or SP6 RNA polymerase using the MAXIscript Kit (Ambion) to produce the HvCESA1 antisense-targeting (466nt ...
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker and then incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker after which they were incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Immunology 2020Quote: ... DNA was stained for 5 minutes in PBS with 1 ug/ml of DAPI and coverslips mounted with Pro-Long Gold (Invitrogen). Imaging was performed using a Leica SPE2 confocal microscope using LAS X version 3.3.0 software (Leica Microsystems ...
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bioRxiv - Immunology 2020Quote: ... The nTreg cells from Foxp3gfp male mice were FACS sorted and expanded in vitro for 5 days by using Dynabeads Mouse T-Activator CD3/CD28 (Invitrogen) in the presence of 500 U/mL recombinant IL-2 ...
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bioRxiv - Immunology 2021Quote: ... PLAT-E cells were washed with 45 ml of PBS and were dissociated from the culture flask by a 5 minute incubation (37°C) with 9 ml of TrypLE reagent (Gibco), which was inactivated by adding 36 ml PBS ...
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bioRxiv - Immunology 2020Quote: ... Tissue was shaken for 10 s before 5 ml of L-15 medium supplemented with 10% foetal calf serum (Invitrogen) was added ...
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bioRxiv - Immunology 2020Quote: ... then incubated another 5 hr at 37 C with additional Protein Transport Inhibitor Cocktail (eBioscience 00-4980-03, Thermo Fisher). Cells were then fixed and permeabilized with BD Cytofix/Cytoperm Kit (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... The lysis was performed in PCR strips by transferring 5 µl of worm pellet into 45 µl of Lysis Solution supplemented with 1:100 DNase I (Ambion). The samples were then lysed by at least 10 freeze-thaw cycles using liquid nitrogen and a warm water bath ...
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bioRxiv - Immunology 2021Quote: ... samples were once again washed three times with 1XPBS+0.2% Triton-X100 for 5 minutes and mounted onto slides with ProLong Gold antifade reading with DAPI (Invitrogen P36935). Images were captured using a Zeiss LSM 710 confocal microscope and receptor expression quantitated using ImageJ.
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bioRxiv - Immunology 2020Quote: ... The vRNA was eluted (45μL) and 5 μL was added in a 0.1 mL fast 96-well optical microtiter plate (Cat.# 4346906, ThermoFisher, CA).
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bioRxiv - Microbiology 2020Quote: ... 1 μL (10μM) reverse primer (ldh4_R, 5’-AATCACAGCAGCCCCTTG-3’) and 1 μL (1 U/μL) Platinum Taq DNA polymerase (Invitrogen, 10966-018) in a 50 μL total reaction volume ...
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bioRxiv - Immunology 2020Quote: ... containing 50 mM dithiothreitol (DTT) for 5 min at 100°C before loading on 8-16% Tris-glycine gels (ThermoFisher). Gels were electrophoresed at RT in running buffer (25 mM Tris ...
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bioRxiv - Immunology 2020Quote: ... Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco, Waltham, MA) to remove excess virus ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were sonicated and aliquots containing 5 x 106 cells were separated on a NuPAGE bis-tris 4–12% gradient polyacrylamide gel (Invitrogen) under reducing conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... Neurons were maintained in a 37°C humidified incubator with 5% CO2 in phenol-free Neurobasal-A medium (Life Technologies) supplemented with B-27 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... The iPSCs were dissociated (DD0) after 4-5 days of maintenance using 0.25% trypsin / 1 mM EDTA (Thermo Fisher Scientific) at 37°C for 2 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 40C in 5% NDS/PBS (Nkx2-1 [abcam Cat. Ab227652] 1:500, St18, 1:1000, Ki67 [Invitrogen Cat ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR was performed using qPCRBIO SyGreen Blue Mix Lo-ROX (PCR biosystems) and the QuantStudio 5 Real Time PCR system (ThermoFisher). The data were analysed using QuantStudio Design and Analysis software (ThermoFisher) ...
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bioRxiv - Cell Biology 2021Quote: ... and 8 mM MgSO4 pH 7.0 (2x PHEM buffer) containing 1% Triton X100 for 5 min and then fixed in 4% (v/v) paraformaldehyde (ThermoFisher) in 2x PHEM buffer at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... 1-2 μg of plasmid was used to transfect 500,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5-10 μL; Thermo Fisher) in OptiMEM (400 μL ...