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Citations for Thermo Fisher :
9451 - 9500 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... jGCaMP7b-XC or jGCaMP7b-XN and 1 μg cDNA encoding CFP (for labelling the soma area and neurites) were transiently transfected into DIV 5-7 cultured cortical neurons by Lipofectamine 2000 (Invitrogen) with a typical protocol according to the manual ...
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bioRxiv - Developmental Biology 2022Quote: ... span down at 200x g for 5 min and resuspended in Aggregation media consisting of KnockOut DMEM/F12 (Gibco, 12660012), 20% KnockOut Serum Replacement (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... we performed 5-ethynyl-2′-deoxyuridine (EdU) staining using the Click-iT EdU Alexa Fluor 647 imaging kit (C10340, Invitrogen). Four-color image acquisition of three replicates per condition was performed on an SP8 confocal microscope (Leica) ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged at ~300g for 5 minutes at room temperature and washed twice in 5ml of prewarmed DPBS (Dulbecco’s phosphate-buffered saline) (Gibco: 14190250) and subsequently seeded at 0.25*106 cells/ml in IMDM ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed three times for 5 minutes with PBS and incubated with 4μg/mL of goat anti-rabbit-Alexa-488 (ThermoFisher Scientific) in the blocking solution for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were dried for 30 mins in a 37°C incubator and washed 3×5 min with 0.1% TritonX-100 in PBS (PBST) and incubated in 10% Horse Serum (ThermoFisher, #26050070), 0.4% TritonX-100 in 1x PBS (blocking buffer ...
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bioRxiv - Biophysics 2022Quote: ... HEK-293 cells cultured in DMEM supplemented with 10% FBS at 37°C with 5% CO2 were co-transfected with hERG1a in pcDNA3 and GFP in pcDNA3 using lipofectamine 3000 (Invitrogen). Cells were plated onto coverslips 14–16 h after transfection and cells with green fluorescence were selected for recordings ...
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bioRxiv - Cancer Biology 2022Quote: The NCI-H460 cell line was obtained from the American Tissue Collection Center (ATCC) and cultivated in a humidified atmosphere at 37°C and 5% CO2 using RPMI medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... and samples were centrifuged at 8000xg for 5 minutes at −9°C in a Sorvall Legend Micro 17R (Thermo Scientific). The supernatant (extract ...
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bioRxiv - Bioengineering 2022Quote: ... and stable pAGDisplay-RBD cell lines were cultured at 37°C in a 5% CO2 atmosphere in DMEM medium (4.5 g/L glucose, L-glutamine; Gibco, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... The dissociated cells and tissue fragments were centrifuged at 1,000 rpm for 5 mins at 4 ℃ and cultured in a T75 tissue-culture flask in a growth medium (advanced DMEM/F12 (Gibco) containing 5% bovine serum (Gibco)) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were incubated in a blocking/permeabilization buffer (0.3% Triton X-100 and 5% normal goat serum (Fisher Scientific) in 1X PBS ...
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bioRxiv - Plant Biology 2022Quote: ... Rosettes were rinse 3 times in distilled water and stained 20 min in a 5% (v/v) Lugol’s iodine solution (Fisher Scientific). After 3 rinses in distilled water ...
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bioRxiv - Cell Biology 2022Quote: ... at standard 5% CO2 and atmospheric O2 at 37°C in DMEM (5.5 mM glucose) supplemented with 10% FBS (Thermofisher #10437010), 50 μg/ml uridine (Sigma-Aldrich #U6381) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was then diluted 1:5 in 1x Assay buffer and processed according to the kit instructions (DNA Damage Competitive ELISA Kit, Invitrogen). The initial DNA concentration used in the assay was optimized to ensure the resulting values fall within the optimum range of kit detection limits (between Std3 and Std6) ...
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bioRxiv - Plant Biology 2022Quote: ... and the amplified DNA fragment and an annealed linker oligo (for GFP: primers 5 and 6) were introduced into the pENTR/D-TOPO vector (Invitrogen; sequences were confirmed by Sanger sequencing using primers 7 and 8 ...
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bioRxiv - Biochemistry 2022Quote: ... Chromatographic separation was carried out on an Aquasil C18 analytical column (4.6 mm x 150 mm, 5 mm; Thermo Scientific). MNA was eluted with the mobile phase consisting of acetonitrile (A ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were washed with TBST three times for 5 minutes and developed with the ECL system (ThermoFisher, Cat No. #34095) according to the manufacturer’s protocols.
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bioRxiv - Cancer Biology 2022Quote: ... All cells were maintained in a humidified 5% CO2 incubator at 37 °C and subcultured by 0.05% trypsin–0.5 mM EDTA solution (Gibco Life Technologies). Once the confluences reached suitable percentages ...
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bioRxiv - Cell Biology 2022Quote: ... was cultured in a gelatin/fibronectin matrix (5 μg fibronectin / 0.02% gelatin (m/v)-Sigma) and Claycomb culture medium supplemented with Glutamax™ (Gibco), 10% (v/v ...
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bioRxiv - Genomics 2022Quote: ... followed by seeding 5×104/cm2 of mouse embryonic fibroblast (MEF) cells (CellBiolabs, CBA-310) in MEF medium (DMEM [Gibco, 11965118] ...
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bioRxiv - Cancer Biology 2022Quote: ... Trp53loxP/loxP mice were incubated at 37°C and 5% CO2 in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher Scientific) containing 10% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... and HeLa-LAP-Spindly cells were grown in a humidified incubator at 37°C and 5% CO2 in DMEM (Gibco) containing 10% FCS Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then washed 3x for 5 minutes with 1xPBS before mounting onto glass slides using ProLong Diamond anti-fade mountant (Invitrogen) and left to dry for 24hrs before imaging ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were incubated for 5 minutes with 1 ml of Supersignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095) and the final blot were imaged using the ‘chemi high-resolution’ setting on a Bio-Rad ChemiDoc MP System.
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bioRxiv - Cancer Biology 2022Quote: ... These three cell lines were grown at 5% CO2 and 37° C in RPMI 1640 without L-Glutamine media supplemented with heat inactivated 10% FBS (Gibco), penicillin/streptomycin (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... The filtrate was spun at 500 x g for 5 min to pellet the cells that were then resuspended in Ham’s F-10 growth media (Thermo Fisher), 20% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells (CCL-2, ATCC) were maintained in T-75 flasks (Falcon) at 37 °C and 5% CO2 in DMEM (Gibco) supplemented with 4.5 g/L glucose ...
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bioRxiv - Cancer Biology 2022Quote: ... and maintained at 37 °C in a humidified atmosphere of 5% CO2 (Stericycle CO2 incubator, Thermo Scientific, Waltham, MA, USA). The cell line was authenticated (STR profiling ...
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bioRxiv - Cell Biology 2022Quote: ... dissociated neurons were resuspended and cultured at 37°C in a humidified incubator with 5% CO2 and 95% O2 in poly-D-lysine coated 6-well plates in neurobasal media (12349015, Gibco) supplemented with 1% GlutaMAX™ Supplement (35050-061 ...
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bioRxiv - Cancer Biology 2022Quote: ... mechanically dissociated into small fragments using narrow aperture glass pipettes then digested briefly for 5 min at 37°C in TrypLE express (Gibco) which was then quenched using DMEM (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... and these cells were cultured by a standard protocol at 37 °C with 5 % CO2 in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin (ThermoFisher).
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bioRxiv - Neuroscience 2020Quote: ... with 1% PBS-T (1% Triton X-100 in 1x PBS) and blocked in 1% PBS-T containing 5% normal goat serum (ThermoFisher) for one hour ...
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bioRxiv - Plant Biology 2021Quote: ... The 10 μL reaction mixture was prepared as follows: 5 μL TaqMan™ Fast Universal PCR Master Mix (Applied Biosystems), 0.2 μL each of forward and reverse primers (0.2 uM) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then spinned down (1000 rcf, 5 min) and equally distributed in wells containing growth medium (DMEM-F12-glutamax [Gibco] ...
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bioRxiv - Neuroscience 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) staining was performed using Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Cells were cultured at 37°C and 5% CO2 in growth medium containing high glucose Dulbecco’s Modified Eagle Medium (ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... and typically passaged at 80-95% confluence using a pre-warmed 0.25% trypsin-EDTA solution for a maximum of 5 minutes (Gibco SH30236.02).
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bioRxiv - Microbiology 2021Quote: ... Peptides were initially trapped in C18 PepMap100 pre-column (300 µm inner diameter x 5 mm, 100A, Thermo Fisher Scientific) in Solvent A (Formic acid 0.1% (v/v) ...
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bioRxiv - Microbiology 2021Quote: Bacteria (~5 × 107 bacteria/ml) added to RPMI-HSA supplemented with 1 μM of Sytox Green Dead Cell stain (Thermofisher) or 30 μM DiOC2 (PromoCell) ...
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bioRxiv - Developmental Biology 2021Quote: ... 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301], 100 μL of Collagenase Type I [480U/mL, Gibco, 17100-017] in 9 mL of PBS [Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... Non-specific binding sites were blocked for 2 h at room temperature with 5% normal SVF (Gibco, ThermoFisher Scientific, France) in 0.1% Triton X-100-PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Non-specific binding sites were blocked for 2 h at room temperature with 5% normal SVF (Gibco, ThermoFisher Scientific, France) in 0.1% Triton X-100-PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed three times for 5 minutes with PBS before incubation with streptavidin with fluorescent tag (Alexa Fluor 647 – Thermofisher) in blocking solution at 1:1000 dilution for 1.5hrs ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were then plated on collagen-coated 35 mm Petri dishes (Biocoat) and maintained in culture at 37°C (95% air/5% CO2) with the following medium: Neurobasal-A (Gibco) completed with L-glutamine (Lonza ...
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bioRxiv - Molecular Biology 2021Quote: For tracking experiments cells were labelled with 5 nM JF549-HaloTag ligand for 15-30 minutes at 37 °C in mouse ES imaging medium (FluoroBrite DMEM (ThermoFisher, 10 % FCS supplemented with nonessential amino acids ...
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bioRxiv - Plant Biology 2021Quote: ... 5 mM MgCl2 and 0.5 mM MnCl2) and quantified by using Pierce™ BCA Protein Assay Kit (23227, Thermo scientific). In the case of protease digestions ...
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bioRxiv - Molecular Biology 2020Quote: ... Untranslated regions were cloned using synthetic DNA (Integrated DNA Technologies) or by isolation using 5′ RACE (RLM-RACE kit, Invitrogen). Template was PCR amplified using Phusion polymerase from the plasmids using the following primers ...
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bioRxiv - Molecular Biology 2020Quote: ... Trypsinized hippocampi were washed two times with the same HBSS buffer before being triturated 5-6 times with a fire-polished glass Pasteur pipette in BME (Invitrogen) supplemented with 10 percent FBS (VWR) ...
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bioRxiv - Molecular Biology 2020Quote: ... the blot was incubated for 5 min with SuperSignal™ West Pico PLUS Chemiluminiscent substrate (Thermo scientific, cat. No. 34577), exposed on film and developed.