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Citations for Thermo Fisher :
9701 - 9750 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... charged by Ni2+ ions or 1 ml Tricorn 5/10 column (Cytiva) packed by Ni-NTA resin (Thermo Fisher Scientific). Columns were equilibrated by the 50 mM Na-PO4 ...
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bioRxiv - Immunology 2021Quote: ... The 96-well plates w preloaded with 9 μL of RT-STA solution: 5 μL of CellsDirect PCR mix (Invitrogen), 0.2 μL of SuperScript-III RT/Platinum Taq mix (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... was added to the cells in half culturing volume (40 μL added) and incubated in the dark for 5 min prior to reading on a Varioskan LUX plate reader (ThermoFisher). Measurements were done on all ten mouse sera samples from each group in at least duplicate ...
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bioRxiv - Immunology 2020Quote: ... and plated in culture dishes (TPP) with 5 x 106 cells/dish in 12 mL of complete RPMI (RPMIc): RPMI (Gibco) with 10 % V/V Hi FCS (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... Mast cells and macrophages were obtained from the peritoneal cavity by lavage with 5 ml cold PBS + EDTA (2 mM, Gibco). Cells were resuspended in RPMI medium containing 10% FCS.
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked with 5% milk in PBS for 1 hour and incubated overnight with primary antibody diluted in PBS which contained 5% milk and 0.2% Tween 20 (BP337, Fisher Scientific). The following day the membrane was rinsed several times with TBST (tris-buffered saline with 0.1% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... volvulus worm to each well of 6-well plates containing 5 ml of DMEM +10% fetal bovine serum (Gibco, A3840002) + 100 u/ml penicillin + 100 ug/ml streptomycin + sodium bicarbonate (2 g/1) ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were heated at 95°C for 5 min and separated on 4520% acrylamide Tris-Glycine gradient gels (Life Technologies). Proteins were transferred to polyvinylidene difluoride membranes (EMD Millipore ...
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bioRxiv - Developmental Biology 2019Quote: ... 50-100 μg of total protein extracts were solubilized in SDS-sample buffer by boiling at 95°C for 5 minutes and analysed by SDS polyacrylamide gel electrophoresis (4-12% NuPAGE gel; Invitrogen). Western blotting was performed using antibodies against Vasa (rat ...
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bioRxiv - Developmental Biology 2019Quote: ... isolated cardiomyocytes were concentrated by 20 × g centrifugation for 5 min and re-suspended in cell culture medium [DMEM (Gibco), 10% FBS ...
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bioRxiv - Biochemistry 2020Quote: ... T-rex 293 cells (approximately 3 × 106 cells per 10-cm dish) were transfected with 5-HT2C wild-type or mutant DNA using Lipofectamine 2000 (Invitrogen) following manufacturer’s protocols ...
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bioRxiv - Genomics 2019Quote: The flasks were incubated for five days at 37°C in 5% CO2 and RNA was isolated using Trizol (Ambion) according to the method described by the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... The percentage of CD4-expressing cells was measured after incubation for 10 minutes with 1 µl of anti-CD4 antibody coupled to Alexa 647 (rat isotype, RM4-5, Invitrogen). For each cell line ...
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bioRxiv - Biochemistry 2019Quote: ... blocked with 5% milk in TBST and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (ThermoFisher MA1-21315, 1:3000). Blots were incubated with primary antibodies overnight at 4 °C with rocking and were then washed (3×5min ...
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bioRxiv - Cell Biology 2019Quote: ... in which the HEPES was replaced with 25 mM sodium bicarbonate and the PVA was replaced with 5% fetal bovine serum (Invitrogen). Media were equilibrated with 5% CO2 and 95% air ...
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bioRxiv - Biochemistry 2019Quote: ... HRP-conjugated secondary antibodies were either inactivated by incubation with sodium azide in 5% skim milk/TBST or stripped with Restore Stripping Buffer (ThermoFisher). The following antibodies were used in this study ...
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bioRxiv - Microbiology 2019Quote: ... the mixture was heated to 65°C for 5 min using the 2720 Thermal Cycler (Applied Biosystems; Foster City, USA) and rapidly cooled on ice afterwards to prevent renaturation ...
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bioRxiv - Immunology 2019Quote: ... isolated lungs were cut into 2-3 mm2 sections and resuspended in 5 mL digestion solution of Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco) supplemented with 5 mM GlutaMax (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: ... centrifuged for 5 minutes at 400 × g and resuspended in B27 medium (Neurobasal supplemented with 1% B27 without vitamin A (Gibco), 2 mM L-glutamine and 0.2% penicillin/streptomycin) ...
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bioRxiv - Bioengineering 2019Quote: ... proteins were transferred onto a nitrocellulose membrane and then blocked overnight while shaken at 4°C in TBST-5% BSA – a TBS Tween-20 (TBST; ThermoFisher) solution containing 5% bovine serum albumin (BSA ...
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bioRxiv - Genetics 2019Quote: ... Primary blood lymphocytes were isolated after venipuncture into a Vacutainer CPT (Becton Dickinson, Franklin Lakes, NJ) per the manufacturer’s recommendations and grown in 5 mL RPMI 1640 (Life Technologies) supplemented with 10% fetal bovine serum (Hyclone ...
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bioRxiv - Physiology 2019Quote: ... Sulfhydrated proteins in the dried bands were reduced with 0.1 mL of 65 mM dithiothreitol (DTT) (Fisher Scientific # BP172-5) at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: ... pelleted again by centrifugation and resuspended at 3−5 × 106 cells/ml in sterile PBS containing 1.25 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; ThermoFisher Scientific) and stored on ice until required ...
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bioRxiv - Microbiology 2019Quote: Images were captured using an EVOS XL Core (Fig 1) or EVOS FL (Fig 5) Cell Imaging System (ThermoFisher Scientific). To measure transfection efficiency ...
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bioRxiv - Microbiology 2019Quote: ... The suspension was centrifuged at 12,000 g for 5 min and the protein supernatant was quantified using the BCA protein assay kit (ThermoFisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Any typical colony was streaked to a trypticase soy agar plate supplemented with 5% defibrinated sheep’s blood (Thermo Scientific-Remel) to confirm isolate purity ...
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bioRxiv - Cancer Biology 2020Quote: ... interphase between upper fat layer and cell pellet was removed and 5 mL of 0.25% trypsin-EDTA (Invitrogen, 25200-056) was added ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were detached using EDTA (0.5 mM) and transferred on uncoated dishes in sphere medium (DMEM:F12, Neurobasal, N2-supplement, B27-supplement, Glutamine (all ThermoFisher Scientific), Insulin (CSBio ...
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bioRxiv - Cancer Biology 2019Quote: ... Mouse organoids were established by enzymatic digestion of GEMM primary prostate tumor tissue in 5 mg/ml Collagenase type II (Gibco) in adDMEM/F12 (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 500 tubuloids in 40 μL differentiation medium with 5% BME were plated in a 384-wells plate using the Multidrop dispenser (Thermofisher) and treated with increasing concentrations of bicalutamide that were always combined with 100 μM cysteamine ...
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bioRxiv - Genomics 2021Quote: ... The cells were stained for 5 minutes at room temperature with LIVE/DEAD Fixable Violet Dead Stain Kit (ThermoFisher L34864). Subsequently ...
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bioRxiv - Genomics 2021Quote: ... 5036-wn) or in low serum medium (DMEM supplemented with 5% FBS) containing 0.1 ng/ μl BMP4 (Thermo Fisher, PHC9534) as described in (Szemes ...
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bioRxiv - Genomics 2021Quote: ... The 293T cells (ATCC, Manassas, Virginia, USA) were cultured in DMEM (Hyclone, USA) with 5% FBS (Gibco BRL. Co. Ltd.) and 1% penicillin-streptomycin (Sangon Biotech ...
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bioRxiv - Genomics 2021Quote: ... First strand cDNA synthesis was done with 2.5-5 ug of polyA(+) RNA and with Superscript III (Invitrogen, #18080-044) using a reporter-RNA specific primer (Oligo 15 ...
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bioRxiv - Genomics 2021Quote: ... The cells were grown in a humified incubator at 37°C and 5% CO2 and split by dissociation by pipetting (CAD cells) or by trypsinizing (TrypLE, ThermoFisher). To induce differentiation into a more neuron-like phenotype ...
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bioRxiv - Microbiology 2021Quote: ... and cDNAs were synthesized using SARS-CoV-2 nucleocapsid (N) reverse primer N660R (5’-AGCAAGAGCAGCATCACCGCCATTGCCAGC-3’) and M-MLV reverse transcriptase (Invitrogen). Then ...
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bioRxiv - Genetics 2020Quote: ... centrifuged for 5 minutes at 400g and resuspended in B27 medium (Neurobasal supplemented with 1% B27 without vitamin A (Gibco), 2 mM L-glutamine and 0.2% penicillin/streptomycin Y27632 (10 µM) ...
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bioRxiv - Immunology 2021Quote: ... 1.75x103 cells were loaded into individual well of a 96 well v-bottom plate in RPMI/5% (v/v) FBS/2mM EDTA/1% (v/v) Penicillin/Streptomycin/1% (v/v) L-Glutamine (ThermoFisher)/0.1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... radius and ulna dependent on the cell numbers required and collected into PBS/5% (v/v) FBS/1% (v/v) Penicillin/Streptomycin (ThermoFisher). All subsequent processing was undertaken in a biological safety level 2 tissue culture hood with sterile reagents ...
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bioRxiv - Cell Biology 2020Quote: ... Metabolites were detected and quantified as area under the curve based on retention time and accurate mass (≤ 5 ppm) using the TraceFinder 3.3 (Thermo Scientific) software.
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bioRxiv - Cell Biology 2020Quote: ... NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit) and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion). For GFP-centrin ...
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bioRxiv - Microbiology 2021Quote: ... or hydrogen peroxide (5 and 10 mM) and incubated with or without 50 μM of diphenyl-1-pyrenylphosphine (DPPP) (ThermoFisher) at 37°C (68 ...
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bioRxiv - Cell Biology 2021Quote: ... and the suspension diluted 1/5 by adding FACS buffer (FB; 2% heat-inactivated Fetal bovine serum (Life Technologies; 26140079), 25 mM HEPES (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in 5% BSA in TBS-T for 30min before incubated with specific primary antibodies and the corresponding secondary antibody (Invitrogen, #A21206 and #A10038 ...
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bioRxiv - Genomics 2021Quote: RNA from individual E10.5 hearts (N=3 or 5 per genotype) was isolated using the RNAqueous-Micro Total RNA Isolation Kit following manufacturer recommended protocols (Invitrogen). cDNA synthesis was performed using random hexamers and SuperScript IV reverse transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... 5 µm particle size) while the analytical column was an Acclaim PepMap 100 (75 μm X 25 cm) (Thermo Scientific). After equilibrating the column in 98% solvent A (0.1% formic acid in water ...
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bioRxiv - Physiology 2021Quote: ... Plates were incubated for 1 h at 37°C with 5% CO2 and were then overlaid with 1X MEM (Gibco) containing 2% FBS and 0.6% Avicel for 2 days ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were cultured at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Thermo Scientific; 31600-034) containing 3.5 g/L D-glucose ...
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bioRxiv - Molecular Biology 2021Quote: ... 37°C in a 5% CO2 incubator with filtered DMEM supplemented with antibiotics (100 IU/mL streptomycin, 100 IU/mL penicillin [Gibco, Invitrogen, Paisley ...
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bioRxiv - Molecular Biology 2021Quote: ... 37°C in a 5% CO2 incubator with filtered DMEM supplemented with antibiotics (100 IU/mL streptomycin, 100 IU/mL penicillin [Gibco, Invitrogen ...