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Citations for Thermo Fisher :
9851 - 9900 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... and denatured for 5 minutes at 94°C prior to capillary electrophoresis (Applied Biosystems 3500 Genetic Analyzer, Thermo Fisher Scientific).
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bioRxiv - Genomics 2021Quote: 0,7 × 106 MEFs were electroporated with 5 µg plasmid DNA using a Neon Transfection System (Thermo Fisher Scientific, Waltham, Massachusetts) with parameters ...
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bioRxiv - Genomics 2019Quote: ... Cells were cultured at 37°C with 5% CO2 and were maintained in Ham’s F10 nutrient mix (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... The polymerase chain reaction and following allelic discrimination were performed on QuantStudio 5 Real-Time PCR System platform (Applied Biosystems).
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bioRxiv - Genetics 2020Quote: ... Real-time quantitative PCR were performed on 1ml of diluted RT reactions (1/5) using SYBR Green kit (Life Technologies) on a One Step Plus thermocycler (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... and cultured at 5% O2 in Dulbecco’s modified essential medium (DMEM)/F12 with B-27 minus vitamin A (Life Technologies), 1% penicillin-streptomycin ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated in the blocking/0.5 % BSA solution containing 1:500 of a secondary antibody (goat anti-chicken, Alexa Fluor 488, Invitrogen, # A-11039; ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... was reconstituted by submerging the vial at 4°C over night and was diluted 1:5 in ice-cold knock-out DMEM (Cat.-No. 10829, Gibco) until material was evenly dispersed ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we stained the formaldehyde preserves with DAPI (4’,6-diamidino-2-phenylindole, final concentration 5 ug/mL, Thermo Scientific, USA), and counted the microbial cells using hemocytometers (DHC-N01 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then washed extensively (5 times) with cold PBS buffer and then lysed with LDS lysis buffer (Invitrogen) for analysis by Western blot ...
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bioRxiv - Genomics 2020Quote: Mutagenized Aβ was then ligated into the linearized plasmid in a 5:1 ratio (insert:vector) using a ligase treatment (T4, Thermo Scientific) overnight ...
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bioRxiv - Microbiology 2021Quote: ... 4 and 6 by washing this side with 100 μL PBS and quantification of the amount of vRNA by first mixing 5 μL wash fluid with 50 μL Cells-to-cDNA II Cell Lysis Buffer (Thermofisher) and heating at 75°C or 15 minutes ...
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bioRxiv - Cancer Biology 2020Quote: Six metastatic patient-derived samples were treated in adherence for 72h in the presence of 5 μM SFX-01 or water (vehicle control) in DMEM/F-12 GlutaMAX medium (GIBCO) containing 10% foetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at a concentration of 1-2×106/mL in 50-100 µL of cold HBSS containing 5% heat-inactivated FBS (Gibco), 100U/mL penicillin ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mg of total protein/sample was combined with 330 μL RIPA-equilibrated PierceTM Streptavidin Magnetic Beads (Thermo Fisher Scientific) and incubated on a rotator at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5 μg of RNA were incubated with ezDNase and cDNA was obtained with SuperScript IV VILO Master Mix (both Invitrogen) following the manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... Oligonucleotide probes from IDT hybridizing to SdsR or 5S rRNA were end-labelled using KinaseMax 5′ end-labelling kit (Ambion). Blots were hybridized overnight with 32P-5′ end-labelled probes at 42°C ...
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bioRxiv - Microbiology 2022Quote: ... for 30 minutes at RT followed by a 5-minute incubation with 300 nM 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then separated by electrophoresis for a short amount of time (5 min) at 200 V on a NuPAGE 4-12% Bis-Tris gel in 1X MES/SDS (Invitrogen) running buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 5% CO2 in a tissue culture incubator (Panasonic) and handled in a Safe 2020 Class II tissue culture cabinet (ThermoFisher).
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bioRxiv - Neuroscience 2022Quote: ... Ss06901209_m1), fibulin-5 (FBLN5, Ss04805024_m11) and samples were run on a QuantStudio 6 Real-Time PCR system (Applied Biosystems #4485694) in technical and biological replicates (n = 3-5) ...
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bioRxiv - Developmental Biology 2022Quote: 5-ethynyl-2-deoxyuridine (EdU) pulse labeling was performed with the Click-IT EdU imaging kit (Life Technologies; Cat#:C10340) as described in (Perillo et al. ...
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bioRxiv - Cell Biology 2022Quote: ... cells were maintained in physiological (5 mM) glucose DMEM media supplemented to 10% FBS and containing antimycin A and antibacterial supplements (Gibco). Drugs are listed in the Supplemental Material section ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 µg of antibody (3 µg for H3K27ac) was added to 5 µg of sonicated chromatin along with Dynabeads Protein A magnetic beads (Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Biophysics 2022Quote: Fibroblasts grown in vitro to the third passage were plated in 6-well tissue culture plates (5 × 105 cells per well) in DMEM (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Microbiology 2022Quote: ... 72°C for 20 seconds and final termination at 72°C for 5 min in a thermocycler (Applied Biosystems, USA). All PCR products were resolved and visualized by 1.5% to 2% w/v agarose gel electrophoresis ...
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bioRxiv - Molecular Biology 2022Quote: ... and a goat anti-rabbit secondary antibody (1:3000, SeraCare, Milford, MA) in 5% bovine serum albumin (BSA, Thermo Fisher) in TBST ...
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bioRxiv - Biochemistry 2022Quote: ... which was hydrolyzed by adding 1.5 μL of 10 units/μL T4 PNK (polynucleotide kinase) and 1 μL RNase-Out solution (40 units/μL, ThermoFisher) to the cleavage reaction above ...
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bioRxiv - Bioengineering 2022Quote: ... resuspended in assay medium (RPMI with 10% FBS and 20 IU/mL IL-2), and centrifuged (300xg, 5 mins) (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... ATCC CCL-10) cells were maintained at 37°C and 5% CO2 in the DMEM medium (Gibco, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biophysics 2022Quote: ... Blots were washed twice for 5 min with TBST and incubated with HRP-coupled anti-rabbit secondary antibody (Thermo Fisher) at 1:5000 dilution for 1 h in the dark ...
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bioRxiv - Biochemistry 2022Quote: LC-MS/MS: Generated peptides were injected into a PepMap C18 5 mm x 300 um column (Thermo Fisher Scientific) at a flow of 25 μL/min with 0.1% TFA mobile phase ...
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bioRxiv - Cancer Biology 2022Quote: ... Each acyl-CoA was quantified by the [M-507+H]+ product ion with a 5 ppm window with Tracefinder 4.1 (Thermo Scientific). Analysts were blinded to sample identity during sample preparation and analysis.
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bioRxiv - Bioengineering 2022Quote: ... Next donkey-anti mouse Alexa Fluor 647 was added to the medium to a final concentration of 1:1000 and incubated on ice for 5 min (Invitrogen). 200 μL of the medium was then introduced to the cells cultured in a well of a 96 well-plate and incubated for 5 min at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed 3 times for 5 minutes each with PBS and mounted using ProLong Diamond anti-fade mountant with DAPI (Invitrogen) and allowed to cure overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25 ...
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bioRxiv - Biochemistry 2022Quote: All mammalian cells were cultured at 37°C in a humidified atmosphere with 5% CO2 in DMEM (Thermo Fisher, 11995073) supplemented with 10% fetal bovine serum (Bio-Techne ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were cast on methacrylate-coated glass slides at various Am/Bis ratios (table S2) with 5 μl far red fluorescent microspheres (F8807, ThermoFisher) added to the prepolymer mixture ...
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bioRxiv - Cancer Biology 2022Quote: ... in 500 μL HEPES (25 mM)/PBS were mixed overnight at 4 °C with 5 μL of 4 μm aldehyde-activated latex beads (A37304, Invitrogen). The beads were blocked with 200 μL of 1% BSA for 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
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bioRxiv - Biochemistry 2022Quote: ... cells were grown at 37°C in 5% CO2 in McCoy’s 5A medium supplemented with 10% FBS superior (Gibco, heat-inactivated, Fisher Scientific), 2 mM L-glutamine (Lonza BioWhittaker ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was washed 3x for 5 min with TTBS and incubated 1 h at RT under agitation with anti-GFP-HRP (Thermofisher) diluted 2,500 fold in 1% BSA in TTBS ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was filtered through 5 µM and 1.2 µM filters and applied to a column containing SulfoLink resin (ThermoFisher, 20402) coupled to PDZ ...
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bioRxiv - Biophysics 2022Quote: ... HEK293 and COS-7 cells were maintained at 37 ºC and 5% CO2 and cultured in Dulbecco’s modified Eagle media (Life Technologies) supplemented with 10% FBS (Hyclone ...
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was first blocked with 5% milk and then incubated overnight with primary antibodies GLS (ThermoFisher, Cat# PA5-40135), IDH1 (Cell Signaling ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos at E1.5 were incubated 15 minutes in M2 + BSA medium supplemented with 5 ng/mL Hoechst (Invitrogen Ref. H3570) to label DNA.
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bioRxiv - Cell Biology 2022Quote: ... and interacting proteins were co-immunoprecipitated with 5 µg of anti-gag antibody per mg of total proteins using Dynabeads protein G (Invitrogen). Proteins were solubilized in Laemmli buffer and separated by SDS–PAGE ...
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bioRxiv - Developmental Biology 2022Quote: ... tissues were washed in PBS with 2% Triton X-100 at 37°C for 5 days and mounted in SlowFade Diamond Antifade Mountant (S36972, Invitrogen). The stained whole mounts were imaged under the two-photon microscope.
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bioRxiv - Cell Biology 2022Quote: ... 5′-CCUGGAUAAUGAUGAAGGA-3′), OSBP (predesigned, cat. no. 4392420) and nontargeting control siRNA (predesigned, cat. no. 4390844) were purchased from Ambion. ON-TARGET plus Human PI4K2A siRNA (predesigned ...
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bioRxiv - Developmental Biology 2022Quote: ... HEK293T cells were routinely cultured at 37°C and 5% CO2 on dishes in DMEM high glucose medium (Gibco #41966) supplemented with 10% Fetal bovine serum (Life technologies #10270-106 ...