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Citations for Thermo Fisher :
9551 - 9600 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... and reverse (5’-ACAGGACATTGACCAACCCA-3’) primers (0.3μM) and 1X premixed Phusion Flash High-Fidelity PCR Master Mix (Thermo Scientific, France). Amplifications were carried out in a thermal cycler LifeEco (BIOER Technology ...
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bioRxiv - Genetics 2022Quote: ... coli MG1693 Thy-which had been grown for 24 hours at 37C with shaking in minimal media containing 20uM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen). The culture consisted of 100 mL M9 ...
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bioRxiv - Genetics 2022Quote: ... Hygromycin-resistant colonies emerging on the surface of the overlay after 3–5 days were excised and transferred to MEA amended with 100 µg/mL of hygromycin B (Invitrogen) (MEA+Hyg100) ...
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bioRxiv - Genetics 2022Quote: The cloned SNP STARRseq library (100ug plasmid DNA/replica) was transiently transfected into LNCaP cells (5 × 107 cells/replica; 3 biological replicas) using the Neon Transfection System (Invitrogen). Cells were grown in RPMI 1640 medium supplemented with 10% FBS and collected 48hrs post electroporation ...
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bioRxiv - Genetics 2022Quote: The fresh skin punch biopsies were dilacerated with scalpels under a sterile area and then transferred into T25 flasks containing 5 mL of AmnioMAX C-100 (Gibco) medium ...
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bioRxiv - Immunology 2022Quote: ... TG2 enzymatic activity was measured using 5-BP crosslinking and was visualized by costaining with AF594-conjugated streptavidin (Invitrogen, S32356). Images were acquired at 20x magnification using a Leica SP8 Laser Scanning Confocal microscope ...
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bioRxiv - Immunology 2022Quote: Surface antibody staining was performed in PBS containing 5% FCS and 1mM EDTA and using a Fixable Aqua Dead Cell (Invitrogen) to determine viability ...
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bioRxiv - Cancer Biology 2022Quote: ... MC38Ifnar1_KO and cells were cultured under standard condition at 37°C in a humid atmosphere with 5% CO2 in DMEM GlutaMAX medium (Gibco) supplemented with 10% FBS (Lonza ...
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bioRxiv - Cancer Biology 2022Quote: ... CT26 and CT26LM3 cells were cultured under standard condition at 37°C in a humid atmosphere with 5% CO2 in RPMI GlutaMAX medium (Gibco) supplemented with 10% FBS (Lonza ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR analysis was performed on an Applied Biosystem™ QuantStudio™ 5 Real-Time PCR system (Thermo Fisher Scientific) using Itaq™ Universal SYBR (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... and lysed by heating for 5 mins at 95°C with Max Bacterial Enhancement Reagent (Invitrogen, cat. no. 46-6036), and stored overnight at -20°C.
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bioRxiv - Cancer Biology 2022Quote: ... and then incubated in the dark at 21°C for 30 minutes with a staining solution of 5 μM DRAQ5 (Invitrogen) and 5 μM Calcein AM (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with PBS for 5 min followed by incubation with Alexa fluor-488 conjugated secondary antibody (ThermoFisher Scientific) for 1hr at room temperature ...
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Understanding molecular mechanisms and predicting phenotypic effects of pathogenic tubulin mutationsbioRxiv - Genetics 2022Quote: HeLa cells were cultured and maintained in a humidified 5% CO2 atmosphere at 37°C in DMEM supplemented with 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) and 10% Fetal Bovine Serum (Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: ... were generated from H9 hESCs and cultured in suspension in the presence of BMP4 (1 ng/mL) and ROCK inhibitor (5 uM) for 24 hours in StemPro-34 medium (Gibco) supplemented with L-glutamine (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were grown at 37°C in 5% CO2 on growth medium consisting of DMEM supplemented with 10% GlutaMAX (Thermofisher), ampicillin ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were resuspended in 1 ml IP lysis buffer [25 mM Tris-HCl pH 7.5 / 150 mM NaCl / 1% NP-40 / 1 mM EDTA / 5% glycerol / Halt protease inhibitor cocktail (Thermo scientific) / Halt phosphatase inhibitor cocktail (Thermo scientific)] and incubated on ice for 1 h ...
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bioRxiv - Genomics 2022Quote: HeLa-JVM cells19 were cultured at 37°C and 5% CO2 in HeLa complete medium (DMEM, Life Technologies, Cat#: 11960044) supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... and cultured at 37°C and 5% CO2 in iPSC media containing KnockOut DMEM (Thermo Fisher Scientific, Cat#: 10829-018), 15% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... Cells were cultured at a density of 5×105 cells/ml in flat-bottom 96-well plates in RPMI-1640 medium (Gibco), supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were excluded by staining for 5 min on ice with LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Invitrogen) before fixation ...
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bioRxiv - Biochemistry 2022Quote: ... coated Corning dishes at 37°C in a 5% CO2 incubator and gently dissociated for passaging at 1:8 ratio every second day with Stem Pro Accutase (Gibco). Cells were washed with Dulbecco’s Phosphate Buffer Saline cell culture grade.
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bioRxiv - Immunology 2022Quote: ... albicans hyphae at roughly MOI 5 were co-incubated with Sytox green (final concentration 1 μM) (Life Technologies, Eugene, OR) for 5 hours at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: ... Wild type wis1+ open reading frame or versions in which Methionine at 395 was substituted with alanine or glycine were synthesised by integrated DNA technologies (IDT) with PstI site at 5’end and KpnI site at 3’end and cloned into pJet1.2 (Thermo Scientific). Wild type wis1+ ORF was amplified by Phusion PCR from NT4 genomic DNA with primers containing PstI and KpnI restriction sites at 5’ end and 3’ end respectively ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with scrambled small interfering RNA or small interfering RNA (siRNA) to silence TMX4 expression (5′-GCAUGGUGUUCUUACGUUUtt-3′, 100 nM per dish, Silencer Select Pre-designed, Ambion). Cells were processed for immunofluorescence or for biochemical analyses after 48h of transfection.
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bioRxiv - Physiology 2022Quote: ... then perfused the lungs with 3 mL of ice-cold DPBS containing Ca2+ and Mg2+ followed by 5 mL of 4% methanol-free formaldehyde (ThermoFisher) in DPBS containing Ca2+ and Mg2+ ...
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bioRxiv - Physiology 2022Quote: ... 1×105 NRK-49F cells were cultured under 37°C and 5% CO2 in plastic culture plates with Dulbecco’s Modified Eagle Medium (DMEM-Low glucose, Invitrogen, USA) containing 5% inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... cells were resuspended in neuronal plating medium (NPM: Minimal Essential Medium with Earl’s Salt and L-glutamine, 0.6% D-glucose, 5% fetal bovine serum; Invitrogen, Carlsbad, USA) and seeded to a concentration of 3.0 × 105 cells per well on EM grids and glass bottom dishes (MatTek corp. ...
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bioRxiv - Cell Biology 2019Quote: The pellets (n = 5/time point/group) were rinsed in PBS and lysed in 500 μl TRIzol (Invitrogen, CA, USA) for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... and MIA PaCa-2 cell lines were a gift from the laboratory of Ben Stanger (University of Pennsylvania) and cultured in DMEM (Mediatech) with 5% FBS (Invitrogen) and 1% antibiotic-antimycotic (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: Caco-2 cells were incubated on sterile coverslips at 37°C in 5% CO2 with 3.5 or 14 μg of PKH26-labeled protozoal EVs (ThermoFisher™) for 1h ...
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bioRxiv - Microbiology 2019Quote: ... The first-strand cDNA was reverse transcribed from 5 μg of total RNA with the SuperScript IV First-Strand Synthesis System (Invitrogen). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Systems Biology 2019Quote: ... Cell lines were grown at 37°C with 5% CO2 in humidified tissue culture incubators (Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Systems Biology 2019Quote: ... 4 µL of medium containing one of 1,833 different bioactive library compounds was then added (final concentration 5 µM) using a Versette robotic liquid handler (ThermoFisher Scientific) and plates were immediately transferred to an IncuCyte Zoom dual color live content imaging system (Model 4459 ...
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bioRxiv - Bioengineering 2019Quote: ... FDB fibers are stained at room temperature for 20 min prior to imaging with 5 µM of the calcium-sensitive dye mag-fluo-4 AM (KD ~22 µM, Invitrogen) dissolved in Tyrode’s buffer.
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bioRxiv - Neuroscience 2019Quote: ... The non-specific binding of antibodies was avoided by incubating the cells with 5% normal goat serum(Thermo Fisher Scientific)/ 0.4% Triton X-100(Sigma)/1% Glycine(Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... and 105 μg AAV-PHP.eB were mixed with 5 mL of Opti-MEM I media (Reduced Serum, GlutaMAX; ThermoFisher Scientific) and 1.1 mL of a solution of 1 mg/mL 25 kDa linear Polyethylenimine (Polysciences ...
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bioRxiv - Immunology 2019Quote: 10% highest and lowest Nur77-eGFP sorted CD4+CD25-CD62LhiCD44lo naïve T cells from SKGNur and WTNur LNs and spleen were labeled with 5 μM Cell Trace Violet according to the manufacturer’s instructions (Life Technologies) before culturing in a 1:10 ratio with 5×105 BALB/c splenic APCs (prepared via Thy1.2+ cell depletion by MACS ...
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bioRxiv - Immunology 2019Quote: 10% highest and lowest Nur77-eGFP sorted CD4+CD25- CD62LhiCD44lo naïve T cells from SKGNur and WTNur LNs and spleen were labeled with 5 μM Cell Trace Violet according to the manufacturer’s instructions (Life Technologies) before culturing with plate-bound αCD3 at the indicated concentration and 2 ug/mL αCD28 in a 96-well flat-bottom plate in complete RPMI medium for 3 days ...
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bioRxiv - Plant Biology 2019Quote: ... and then rotated for another 5 min before loading the strip into the well of a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen).
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... cells were centrifuged for 5 min at 1400 rpm and incubated at 37°C for 2 min in sterile RBC lysis solution (Invitrogen). Lysis was arrested by adding 30 ml PBS ...
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bioRxiv - Pathology 2019Quote: ... Aliquot concentration was determined by enumerating colony growth on agar plates with 5% sheep blood (Remel, Thermo Fisher, Lenexa, KS) at the time of freezing ...
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bioRxiv - Cell Biology 2019Quote: ... Ecadherin-GFP cells and α-catenin KD MDCK cells were maintained in media containing 5 μg/ml geneticin (Life Technologies).
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bioRxiv - Systems Biology 2019Quote: Jurkat T cells (ATTC TIB-152) and K562 cells (ATCC CCL-243) were maintained in 5% CO2 environment in RPMI (GIBCO) media supplemented with 10% fetal bovine serum ...
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bioRxiv - Biophysics 2019Quote: QDs were functionalized with benzyl guanine by mixing 5 μl of 8 μM amino (PEG) QDs emitting at 655 nm (ThermoFisher) with 2 μl of 20 mM BG-GLA-NHS (NEB ...
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bioRxiv - Systems Biology 2019Quote: Metabolites were detected and quantified as area under the curve based on retention time and accurate mass (≤ 5 ppm) using the TraceFinder 3.3 (Thermo Scientific) software ...
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bioRxiv - Molecular Biology 2019Quote: ... At least 5 µg of mRNA was purified from total RNA using the Poly(A)Purist MAG kit (AM1922, Ambion). mRNAs were fragmented for 3 minutes at 70 °C using a Zinc-based alkaline fragmentation reagent (AM8740 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We used 300 ng of purified PCR product to set a BPII recombination reaction (5 μL) into the Gateway Entry Vector pDONR201 (150 ng) according to the manufacturer’s instructions (Invitrogen, USA). BpII reaction mix was incubated overnight at 25°C ...
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bioRxiv - Cell Biology 2020Quote: ... pCR2-TUB1 consists of the region of the TUB1 locus from the intron (situated close the 5’ end of the gene) to 385 bp after the stop codon cloned into the pCR2 vector (Invitrogen). The HIS3 gene expression cassette was ligated into the BsrGI site within the 3’ untranslated region of the TUB1 sequence within pCR2 (pCR2-TUB1) ...
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bioRxiv - Immunology 2019Quote: ... The suspension was shaken for 10 s before addition of 5 ml of L-15 medium supplemented with 10% foetal calf serum (Invitrogen) and centrifugation at 400 g for 5 min ...