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Citations for Thermo Fisher :
9951 - 10000 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: Images were captured using an EVOS XL Core (Fig 1) or EVOS FL (Fig 5) Cell Imaging System (ThermoFisher Scientific). To measure transfection efficiency ...
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bioRxiv - Microbiology 2019Quote: ... The suspension was centrifuged at 12,000 g for 5 min and the protein supernatant was quantified using the BCA protein assay kit (ThermoFisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Any typical colony was streaked to a trypticase soy agar plate supplemented with 5% defibrinated sheep’s blood (Thermo Scientific-Remel) to confirm isolate purity ...
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bioRxiv - Cancer Biology 2020Quote: ... interphase between upper fat layer and cell pellet was removed and 5 mL of 0.25% trypsin-EDTA (Invitrogen, 25200-056) was added ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were detached using EDTA (0.5 mM) and transferred on uncoated dishes in sphere medium (DMEM:F12, Neurobasal, N2-supplement, B27-supplement, Glutamine (all ThermoFisher Scientific), Insulin (CSBio ...
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bioRxiv - Cancer Biology 2019Quote: ... Mouse organoids were established by enzymatic digestion of GEMM primary prostate tumor tissue in 5 mg/ml Collagenase type II (Gibco) in adDMEM/F12 (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 500 tubuloids in 40 μL differentiation medium with 5% BME were plated in a 384-wells plate using the Multidrop dispenser (Thermofisher) and treated with increasing concentrations of bicalutamide that were always combined with 100 μM cysteamine ...
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bioRxiv - Genomics 2021Quote: ... The cells were stained for 5 minutes at room temperature with LIVE/DEAD Fixable Violet Dead Stain Kit (ThermoFisher L34864). Subsequently ...
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bioRxiv - Genomics 2021Quote: ... 5036-wn) or in low serum medium (DMEM supplemented with 5% FBS) containing 0.1 ng/ μl BMP4 (Thermo Fisher, PHC9534) as described in (Szemes ...
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bioRxiv - Genomics 2021Quote: ... The 293T cells (ATCC, Manassas, Virginia, USA) were cultured in DMEM (Hyclone, USA) with 5% FBS (Gibco BRL. Co. Ltd.) and 1% penicillin-streptomycin (Sangon Biotech ...
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bioRxiv - Genomics 2021Quote: ... First strand cDNA synthesis was done with 2.5-5 ug of polyA(+) RNA and with Superscript III (Invitrogen, #18080-044) using a reporter-RNA specific primer (Oligo 15 ...
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bioRxiv - Genomics 2021Quote: ... The cells were grown in a humified incubator at 37°C and 5% CO2 and split by dissociation by pipetting (CAD cells) or by trypsinizing (TrypLE, ThermoFisher). To induce differentiation into a more neuron-like phenotype ...
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bioRxiv - Microbiology 2021Quote: ... and cDNAs were synthesized using SARS-CoV-2 nucleocapsid (N) reverse primer N660R (5’-AGCAAGAGCAGCATCACCGCCATTGCCAGC-3’) and M-MLV reverse transcriptase (Invitrogen). Then ...
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bioRxiv - Genetics 2020Quote: ... centrifuged for 5 minutes at 400g and resuspended in B27 medium (Neurobasal supplemented with 1% B27 without vitamin A (Gibco), 2 mM L-glutamine and 0.2% penicillin/streptomycin Y27632 (10 µM) ...
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bioRxiv - Immunology 2021Quote: ... 1.75x103 cells were loaded into individual well of a 96 well v-bottom plate in RPMI/5% (v/v) FBS/2mM EDTA/1% (v/v) Penicillin/Streptomycin/1% (v/v) L-Glutamine (ThermoFisher)/0.1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... radius and ulna dependent on the cell numbers required and collected into PBS/5% (v/v) FBS/1% (v/v) Penicillin/Streptomycin (ThermoFisher). All subsequent processing was undertaken in a biological safety level 2 tissue culture hood with sterile reagents ...
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bioRxiv - Cell Biology 2020Quote: ... Metabolites were detected and quantified as area under the curve based on retention time and accurate mass (≤ 5 ppm) using the TraceFinder 3.3 (Thermo Scientific) software.
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bioRxiv - Cell Biology 2020Quote: ... NotI-linearized plasmids were purified (QIAquick Gel Extraction Kit) and the 5’-capped mRNAs were synthesized using SP6 mMessenger mMachine kit (Ambion). For GFP-centrin ...
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bioRxiv - Microbiology 2021Quote: ... or hydrogen peroxide (5 and 10 mM) and incubated with or without 50 μM of diphenyl-1-pyrenylphosphine (DPPP) (ThermoFisher) at 37°C (68 ...
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bioRxiv - Cell Biology 2021Quote: ... and the suspension diluted 1/5 by adding FACS buffer (FB; 2% heat-inactivated Fetal bovine serum (Life Technologies; 26140079), 25 mM HEPES (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in 5% BSA in TBS-T for 30min before incubated with specific primary antibodies and the corresponding secondary antibody (Invitrogen, #A21206 and #A10038 ...
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bioRxiv - Genomics 2021Quote: RNA from individual E10.5 hearts (N=3 or 5 per genotype) was isolated using the RNAqueous-Micro Total RNA Isolation Kit following manufacturer recommended protocols (Invitrogen). cDNA synthesis was performed using random hexamers and SuperScript IV reverse transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... 5 µm particle size) while the analytical column was an Acclaim PepMap 100 (75 μm X 25 cm) (Thermo Scientific). After equilibrating the column in 98% solvent A (0.1% formic acid in water ...
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bioRxiv - Physiology 2021Quote: ... Plates were incubated for 1 h at 37°C with 5% CO2 and were then overlaid with 1X MEM (Gibco) containing 2% FBS and 0.6% Avicel for 2 days ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were cultured at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Thermo Scientific; 31600-034) containing 3.5 g/L D-glucose ...
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bioRxiv - Molecular Biology 2021Quote: ... 37°C in a 5% CO2 incubator with filtered DMEM supplemented with antibiotics (100 IU/mL streptomycin, 100 IU/mL penicillin [Gibco, Invitrogen, Paisley ...
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bioRxiv - Molecular Biology 2021Quote: ... 37°C in a 5% CO2 incubator with filtered DMEM supplemented with antibiotics (100 IU/mL streptomycin, 100 IU/mL penicillin [Gibco, Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... was mixed with 300 μL of bacterial overnight culture and 5 mL of molten soft agar (MSM buffer containing 0.4% TopVision Low Melting Point Agarose (ThermoFisher Scientific)) cooled to 32°C ...
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bioRxiv - Neuroscience 2020Quote: ... Retinas were maintained in culture (37°C, 5% CO2) fed by Neurobasal-A media supplemented with 2 mM L-glutamate (GlutaMAX, Gibco) 2 % B27 ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were kept in retrieval solution until reaching room temperature and washed 2 × 5 min with Tris-buffered saline (TBS; ThermoFisher) plus 0.3% (v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Fisher) containing 1x protease inhibitor cocktail III (Calbiochem ...
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bioRxiv - Biochemistry 2021Quote: ... and 300 mM NaCl] was incubated with 20-fold excess (1 mM final concentration) of tetramethylrhodamine-5-maleimide (TMR) (Invitrogen) at room temperature in the dark for 2–4 h ...
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bioRxiv - Microbiology 2021Quote: ... were seeded into one well of a six-well plate and were transfected with 600 ng of plasmid with 5 μL of Lipofectamine 2000 (Invitrogen) in 200 μL of OptiMEM (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 5×104 parental control or transporter containing cells were incubated with 0.25μM Calcein-AM (C3100MP ThermoFisher Scientific, Walthman, MA, USA) in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1 M MgCl2, 0.1M Tris HCL, 1% Tx-100, 1x sBlock, 1% sodium deoxycholate, 5 units/mL DNAse I – Thermo Scientific #89836 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5 ng of the Renilla Luciferase internal control plasmid (pRT-TK) were co-transfected by the Lipofectamine 2000 Reagent (Invitrogen). Forty-eight hours after plasmid transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Cas9/sgRNA RNP complex was assembled just prior to injection and after 5 min incubation at room temperature 200 pg of Cas9 protein (Invitrogen) and 100 pg of sgRNA were co-injected into one-cell stage Tg(fli1ep:Lifeact-EGFP)zf495;Tg(kdr-l:ras-mCherry)s916 embryo ...
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bioRxiv - Biochemistry 2021Quote: The input (total Nav1.5 expression) and biotinylated (cell surface-expressed Nav1.5) samples were separated on NuPAGE 4–12% Bis-Tris gels (Invitrogen, ThermoFisher) and transferred to nitrocellulose membrane (iBlot transfer system ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were dried for 20 min at room temperature then resuspended in 5 μL of 1 M Tris and 10 μL of protein loading buffer (2x NuPAGE LDS sample buffer (Invitrogen), 715 mM 2-mercaptoethanol) ...
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bioRxiv - Biochemistry 2021Quote: 24.5 μL of 5 μM solution of every protein was prepared in 96-well plate MicroAmp Optical plate (Applied biosystems), and incubated at room temp ...
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bioRxiv - Immunology 2021Quote: Thymocytes and LN T cells were cultured at 37°C with 5% CO2 in RPMI-1640 (Gibco, Invitrogen Corporation, CA) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: Thymocytes and LN T cells were cultured at 37°C with 5% CO2 in RPMI-1640 (Gibco, Invitrogen Corporation, CA) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... and human phosphatidylinositol 4-phosphate 5-kinase type-1 beta (hPIP5KB; Uniprot #O14986) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). The gene encoding yeast Mss4 (Uniprot #P38994 ...
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bioRxiv - Microbiology 2020Quote: ... and A549 (ATCC® CCL-18) (verified mycoplasma free) were grown at 37°C with 5% CO2 in Dulbecco’s Modified Eagle’s Medium (Gibco, Dublin, Ireland) supplemented with 10 % fetal bovine serum and Penicillin-Streptomycin solution (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Then, 500 μL of ice-cold wash buffer2 (1x PBS, 5% trehalose, 0.5 mg/ml ULTRAPURE BSA (Thermo fisher, AM2616)) was added ...
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bioRxiv - Developmental Biology 2021Quote: ... sections were incubated in DAPI (used at 2 μg/ml in PBS) for 5 min to counterstain nuclei and mounted in Prolong Gold antifade reagent (Invitrogen). Proprietary (Advanced Cell Diagnostics ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5 µg plasmid DNA and 5 µl TransIT-2020 reagent were added into 250 µl of Opti-MEMI Reduced-Serum Medium (Gibco). After adding the mixture into each well ...
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bioRxiv - Cancer Biology 2020Quote: ... Human primary AML cell were grown at 37°C with 5% CO2 in Iscove’s Modified Dulbecco’s Media with Glutamax (IMDM, Gibco, Life Technologies) supplemented with 20% fetal calf serum (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected pregnant mice intraperitoneally with 100 μL of 5-ethynyl-2’-deoxyuridine (EdU, Click-iT EdU Alexa Fluor 647 Imaging Kit, Invitrogen) at 1mg/mL ...