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Citations for Thermo Fisher :
9751 - 9800 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the cell suspension was centrifuged at 200 g for 5 minutes and the resulting pellet was resuspended in 1 mL of DMEM (Gibco) supplemented with 10% FBS and 1% antibiotic-antimycotic (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... Then slides were washed 3×5’ in TBS and incubated in donkey-α-sheep-488 (1:500, Life Technologies, A11015) in TBS for 90’ ...
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bioRxiv - Neuroscience 2021Quote: ... were plated at 2.5×104 cells/ well of a 96 well plate in HAP1 media (Iscove’s Modified Dulbecco’s Medium (IMDM) (Gibco 12440-053) + 20% FBS (Gibco A38400-01) ...
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bioRxiv - Pathology 2021Quote: ... Samples were transferred to a PepMap 100 C18 trap column (100 µm x 2 cm, 5 µM particles, Thermo Scientific) and separated on an analytical column (PepMap RSLC C18 ...
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bioRxiv - Microbiology 2021Quote: ... target cells were labelled with 5 µM of carboxyfluorescein succinimidyl ester (CFSE) from a CFSE cell proliferation kit (Invitrogen, C34554) for 5 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR mixtures were assembled using 50 ng or 5 µl cDNA and PowerUp SYBR Green Master Mix (Applied Biosystems). For VEEV detection ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were seeded on a 6-well plate with 2 ml of DMEM medium supplemented with 5% horse serum (Gibco). At 24 hours after transfection ...
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bioRxiv - Biochemistry 2020Quote: ... 2020) were generated by PCR-amplifying 5’ UTR sequences from pRF plasmids using AccuPrime Pfx DNA Polymerase (Thermo, Invitrogen, 12344024). pSP73-4xS1m(MCS ...
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bioRxiv - Biochemistry 2020Quote: ... washed twice in PBS and resuspended in around 0.5 mL Staining Solution (5 μg/mL propidium iodide (P3566, ThermoFisher Scientific), 100 μg/mL RNase A (R5503-100MG ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then washed 3x in HBSS and dissociated by pipetting up and down 15-20 times through a P1000 pipette in growth media (Neurobasal media supplemented with 5 % FBS, 500 nM L-glutamine, B27 (Gibco) and Pen-strep ...
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bioRxiv - Bioengineering 2021Quote: ... followed by buffer exchange using the 0.5 ml centrifugal filter described above (cat. no. UFC503024, MilliporeSigma) and 10 mM ammonium acetate buffer (cat. no. AM9070G, Invitrogen).
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bioRxiv - Bioengineering 2021Quote: T cells were cultured in OpTimizer CTS T Cell Expansion SFM containing 5% CTS Immune Cell SR (ThermoFisher, Waltham, MA), L-Glutamine ...
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bioRxiv - Immunology 2021Quote: ... 4 million splenocytes cells were stimulated ex vivo for 3 hours at 5% CO2 at 37°C in Minimum Essential Media (Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2020Quote: ... was analyzed using an Agilent 1100 HPLC system equipped with a MAbPac HIC-Butyl column (4.6 × 100 mm, 5 µm, Thermo Scientific). Elution conditions were as follows ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were analyzed using an Agilent 1100 HPLC system equipped with a MAbPac SEC analytical column (4.0 × 300 mm, 5 µm, Thermo Scientific). Elution conditions were as follows ...
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bioRxiv - Biophysics 2021Quote: ... at 37 °C in a 5% (v/v) CO2 humidified environment (Forma Steri-Cycle CO2 incubator, Thermo Fisher Scientific, Singapore).
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bioRxiv - Neuroscience 2022Quote: ... Reverse transcription of polyA mRNA from 5 µg total DNA-free RNA was performed using Superscript First Strand Synthesis (Invitrogen) with Oligo-dT primers ...
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bioRxiv - Microbiology 2022Quote: Hemolymph sporozoites collected on days 19 to 24 post-infection were centrifuged for 5 min at 450 x g and 4 °C in an 8-well Lab-Tek chamber slide (Nunc) or a 384-well plate (Greiner) ...
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bioRxiv - Neuroscience 2022Quote: ... with added penicillin/streptomycin for 30 min at 37°C and 5% CO2 and then incubated with 50 μg/ml transferrin-Alexa-568 (ThermoFisher) in complete culture media for 20 min at 4°C ...
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bioRxiv - Physiology 2022Quote: ... An aliquot of 100 μL was subsequently derivatized using a final concentration of 10 mM aniline and 5 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (ThermoFisher) for 2 h at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... boiled for 5 min and size-fractionated by gel electrophoresis on NuPAGE 10% Bis-Tris 1.5-mm gels (Life Technologies) in 2-(N-morpholino ...
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bioRxiv - Developmental Biology 2022Quote: ... Merril 108 and Tcf7-/- mESCs previously generated in the laboratory 37 were cultured at 37°C and 5% CO2 on gelatin-coated plates in DMEM (Gibco), 15% fetal bovine serum ...
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bioRxiv - Genetics 2022Quote: ... as well as fibroblasts and SH-SY5Y cells treated for 5 hours with 100 µM oleic acid (OA) following over-night starvation in Opti-MEM (ThermoFisher). OA is a potent inducer of lipid droplet formation (55) ...
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bioRxiv - Microbiology 2022Quote: ... siRNA reverse transfection was performed by mixing 1.2 µl siRNA 5 µM with 1 µl lipofectamine RNAiMAX transfection reagent (Invitrogen, ThermoFisher Scientific) to a final volume of 100 µl with Opti-MEM® (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Cell lines were grown at 37°C and 5% CO2 in high-glucose Dulbecco’s Modified Eagles Medium (Thermo Fisher Scientific) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Physiology 2022Quote: ... The DMEM medium was supplemented with 5% penicillin/streptomycin resistance solution (Sangon, China) and 10% fetal bovine serum (Gibco, USA). The cells were grown at 37 °C with 5% CO2 and sub-cultured every 2–3 days ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T helper cell subsets were identified using the antibodies listed above and the addition of anti-IL-5 (for Th2) and anti-IFNγ (for Th1) after permeabilization (Invitrogen). Gates were set according to Fluorescence Minus One (FMO ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and reverse (5’-ACAGGACATTGACCAACCCA-3’) primers (0.3μM) and 1X premixed Phusion Flash High-Fidelity PCR Master Mix (Thermo Scientific, France). Amplifications were carried out in a thermal cycler LifeEco (BIOER Technology ...
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bioRxiv - Genetics 2022Quote: ... coli MG1693 Thy-which had been grown for 24 hours at 37C with shaking in minimal media containing 20uM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen). The culture consisted of 100 mL M9 ...
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bioRxiv - Genetics 2022Quote: ... Hygromycin-resistant colonies emerging on the surface of the overlay after 3–5 days were excised and transferred to MEA amended with 100 µg/mL of hygromycin B (Invitrogen) (MEA+Hyg100) ...
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bioRxiv - Genetics 2022Quote: The cloned SNP STARRseq library (100ug plasmid DNA/replica) was transiently transfected into LNCaP cells (5 × 107 cells/replica; 3 biological replicas) using the Neon Transfection System (Invitrogen). Cells were grown in RPMI 1640 medium supplemented with 10% FBS and collected 48hrs post electroporation ...
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bioRxiv - Genetics 2022Quote: The fresh skin punch biopsies were dilacerated with scalpels under a sterile area and then transferred into T25 flasks containing 5 mL of AmnioMAX C-100 (Gibco) medium ...
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bioRxiv - Immunology 2022Quote: ... TG2 enzymatic activity was measured using 5-BP crosslinking and was visualized by costaining with AF594-conjugated streptavidin (Invitrogen, S32356). Images were acquired at 20x magnification using a Leica SP8 Laser Scanning Confocal microscope ...
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bioRxiv - Immunology 2022Quote: Surface antibody staining was performed in PBS containing 5% FCS and 1mM EDTA and using a Fixable Aqua Dead Cell (Invitrogen) to determine viability ...
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bioRxiv - Cancer Biology 2022Quote: ... MC38Ifnar1_KO and cells were cultured under standard condition at 37°C in a humid atmosphere with 5% CO2 in DMEM GlutaMAX medium (Gibco) supplemented with 10% FBS (Lonza ...
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bioRxiv - Cancer Biology 2022Quote: ... CT26 and CT26LM3 cells were cultured under standard condition at 37°C in a humid atmosphere with 5% CO2 in RPMI GlutaMAX medium (Gibco) supplemented with 10% FBS (Lonza ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR analysis was performed on an Applied Biosystem™ QuantStudio™ 5 Real-Time PCR system (Thermo Fisher Scientific) using Itaq™ Universal SYBR (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... and lysed by heating for 5 mins at 95°C with Max Bacterial Enhancement Reagent (Invitrogen, cat. no. 46-6036), and stored overnight at -20°C.
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bioRxiv - Cancer Biology 2022Quote: ... and then incubated in the dark at 21°C for 30 minutes with a staining solution of 5 μM DRAQ5 (Invitrogen) and 5 μM Calcein AM (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with PBS for 5 min followed by incubation with Alexa fluor-488 conjugated secondary antibody (ThermoFisher Scientific) for 1hr at room temperature ...
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Understanding molecular mechanisms and predicting phenotypic effects of pathogenic tubulin mutationsbioRxiv - Genetics 2022Quote: HeLa cells were cultured and maintained in a humidified 5% CO2 atmosphere at 37°C in DMEM supplemented with 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) and 10% Fetal Bovine Serum (Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: ... were generated from H9 hESCs and cultured in suspension in the presence of BMP4 (1 ng/mL) and ROCK inhibitor (5 uM) for 24 hours in StemPro-34 medium (Gibco) supplemented with L-glutamine (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were grown at 37°C in 5% CO2 on growth medium consisting of DMEM supplemented with 10% GlutaMAX (Thermofisher), ampicillin ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were resuspended in 1 ml IP lysis buffer [25 mM Tris-HCl pH 7.5 / 150 mM NaCl / 1% NP-40 / 1 mM EDTA / 5% glycerol / Halt protease inhibitor cocktail (Thermo scientific) / Halt phosphatase inhibitor cocktail (Thermo scientific)] and incubated on ice for 1 h ...
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bioRxiv - Genomics 2022Quote: HeLa-JVM cells19 were cultured at 37°C and 5% CO2 in HeLa complete medium (DMEM, Life Technologies, Cat#: 11960044) supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... and cultured at 37°C and 5% CO2 in iPSC media containing KnockOut DMEM (Thermo Fisher Scientific, Cat#: 10829-018), 15% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... Cells were cultured at a density of 5×105 cells/ml in flat-bottom 96-well plates in RPMI-1640 medium (Gibco), supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were excluded by staining for 5 min on ice with LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Invitrogen) before fixation ...
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bioRxiv - Biochemistry 2022Quote: ... coated Corning dishes at 37°C in a 5% CO2 incubator and gently dissociated for passaging at 1:8 ratio every second day with Stem Pro Accutase (Gibco). Cells were washed with Dulbecco’s Phosphate Buffer Saline cell culture grade.
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bioRxiv - Immunology 2022Quote: ... albicans hyphae at roughly MOI 5 were co-incubated with Sytox green (final concentration 1 μM) (Life Technologies, Eugene, OR) for 5 hours at 37°C with 5% CO2 ...