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Citations for Thermo Fisher :
9551 - 9600 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... A burr hole was made to inject the fluorescent muscimol Bodipy(R)-TMR(X) (500 μM in cortex buffer with 5% DMSO, Invitrogen) in the medial part of the posterior thalamic nucleus (POm) ...
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bioRxiv - Neuroscience 2020Quote: ... Invitrogen) were applied for 5 h in antibiotic- and serum-free Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) and cells were then grown in respective cell culture media ...
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bioRxiv - Microbiology 2021Quote: ... Transferred RNA was UV-crosslinked and hybridized overnight with 100 ng/mL of 5′ biotinylated DNA probe (Table S3) in ULTRAhyb (Ambion) hybridization buffer at 42°C ...
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bioRxiv - Neuroscience 2020Quote: Culture medium was replaced with Fluo-4 AM (5 μM) or X-Rhod-1 AM (10 μM) (both from Invitrogen) working solution in Ca2+ solution (pH 7.4) ...
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bioRxiv - Genomics 2020Quote: ... velifer embryonic fibroblasts into 6 well plates and transfected with the resulting Cas9-gRNA vector pair (5 μg DNA each, for a total of 10 μg DNA) via electroporation (ThermoFisher Neon Transfection System #MPK10025 ...
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bioRxiv - Microbiology 2021Quote: Host shutoff activity of 1918 H1N1 WT PA-X on host protein synthesis was determined by transiently transfecting HEK293T cells (5 × 104cells/well, 24-well plate format, triplicates) using Lipofectamine 2000 (LPF2000, Invitrogen) with increasing concentrations (0 ...
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bioRxiv - Neuroscience 2020Quote: ... MEF were grown as monolayers at 37°C with 5% CO2 in DMEM supplemented with 10% serum and MEM non-essential amino acids (Gibco), or with 5% delipidated fetal bovine serum and 35 μM oleic acid ...
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bioRxiv - Microbiology 2021Quote: ... RNA Stop Solution (5% phenol, 95% ethanol) was added and total RNA was extracted using TRI reagent (Thermo Fisher Scientific). RNA was treated with TURBO DNase (Thermo Fischer Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: For quantification of target expression, HPAECs (Day 0, Day 5, Day 10 of chip culture) were recovered using TrypLE Express Enzyme (Gibco) at 37°C for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... The flow-through was centrifuged and resuspended in 5 ml of red blood cell lysis buffer (Invitrogen, USA, 00-4300) for 10 min ...
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bioRxiv - Immunology 2021Quote: ... forward 5’-TAACCGGTATGGGCATACTGAGCTTTCT-3’ (contains the AgeI restriction site) and reverse 5’-TAGGATCCAATCCTGTTCTGGTCGTCG-3’ (contains the BamHI restriction site) and the PCR product was cloned into pCRBlunt (Invitrogen) and sequenced ...
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bioRxiv - Biophysics 2021Quote: ... we wash the blot 3 times with TBST and soak for 5 min in chemiluminescent substrate (Thermo Scientific catalog # 34080).
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto a trapping cartridge (Acclaim PepMap C18 100Å, 5 mm x 300 μm i.d., 160454, Thermo Scientific) in a mobile phase of 2% ACN ...
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bioRxiv - Microbiology 2021Quote: ... using a HA-specific primer (5’-GTCCTTGCGACTG-3’) and a RevertAid first-strand cDNA synthesis kit (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... using a HA-specific primer (5’-GTCCTTGCGACTG-3’) and a RevertAid first-strand cDNA synthesis kit (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and human hepatoma Huh7.5 cells83 were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (Invitrogen, Paisley, UK) supplemented with 10% heat inactivated fetal bovine serum (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18, 5 um particles, 20 mm length, ThermoFisher) at 5 µL/min ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were immediately fixated by adding two times concentrated fixative (5 mM dithiobis(succinimidyl propionate) (DSP) (Thermo Fisher Scientific, USA) and 5 mM succinimidyl 3-(2-pyridyldithio)propionate (SPDP ...
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bioRxiv - Microbiology 2021Quote: ... Samples were concentrated on an Acclaim PepMap 100 (100 μm × 2 cm, nanoViper, C18, 5 μm, 100 Å; Thermo Scientific) trap column and separated on an Acclaim PepMap RSLC (75 μm × 50 cm ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed three times for 5 min and incubated with 1:3000 dilution of horseradish peroxidase-conjugated Goat anti-mouse IgG Fc secondary antibody (Invitrogen) for 1 h ...
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bioRxiv - Plant Biology 2021Quote: ... using 5 μgr Diagenode Ab C15410004 H3K9ac antibody and a mix of 25/25 μl of protein-A/G dynabeads (Invitrogen). 5-10 ng of DNA per sample were used for library preparation with Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences ...
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bioRxiv - Plant Biology 2021Quote: ... ISR1 CDS with a 5’ EcoRI restriction site and 3’ BamHI restriction site was amplified using Phusion (Thermo Fisher Scientific) and ISR1 start + EcoRI FOR and ISR1 no stop + BamHI REV primers (Table S1) ...
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bioRxiv - Microbiology 2021Quote: ... siRNA reverse transfection was performed by mixing 1.2 μl siRNA 5 μM with 1 μl lipofectamine RNAiMAX transfection reagent (Invitrogen, ThermoFisher Scientific) to a final volume of 100 μl with Opti-MEM® (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed and stained for 4 h in 16% PFA solution containing 5 μg mL−1 Hoechst 33258 nuclear stain (Invitrogen). The plates were then imaged using an Operetta High Content Imaging System (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... and MDCK-NS1-GFP cells were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco®) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... were grown under standard conditions at 37°C at 5% CO2 in DMEM supplemented with penicillin (100 U/ml) and streptomycin (100 mg/ml) (Gibco). To investigate calcification in the GC1 cells ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed 3 × (MBL2 wash buffer) and stained with DAPI (600 nM in MBL2 wash buffer; 5 min, RT; ThermoFisher) before imaging ...
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bioRxiv - Microbiology 2020Quote: ... The wells were then washed 5 times with phosphate-buffered saline (PBS) + 0.05% Tween 20 and blocked with SEA BLOCK blocking buffer (Thermo Scientific) in the first panning round and 5% milk powder in PBS in the second panning round ...
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bioRxiv - Molecular Biology 2022Quote: ... with FLAG sequence flanking at 5’ end of the primer and cloned in Hind III and Xba I site of pCDNA3.1V5-His (Invitrogen, USA). Clones were sequenced with T7 and BGH primer using ABI BigDyeTerminator V3.1 cycle sequencing kit followed by automated sequencing in ABI 3130 Genetic Analyzer according to manufacturer’s protocol.
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged for 5 min at 4,000 x g and suspended in ProLong™ Live Antifade Reagent (ThermoFisher Scientific). For imaging ...
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bioRxiv - Microbiology 2020Quote: ... trapped and washed on a precolumn (C18 PepMap 100, 5µm, 300 µm i.d. x 5 mm, 100 Å, Thermo Scientific) for 3 min at a flow rate of 30 μL/min with 100% buffer A (0.1% formic acid in HPLC grade water) ...
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bioRxiv - Cancer Biology 2020Quote: 1 μg total RNA from healthy and OS samples was used for 5’ and 3’ RACE reactions with the FirstChoice RLM-RACE kit (Ambion) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: MDA-MB-231 cells were plated for 1 to 5 hrs on Oregon Green 488 (OR488) or AF594-conjugated cross-linked gelatin (Invitrogen) in EBSS or CM medium in the presence or absence of rapamycin as previously described 38 ...
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bioRxiv - Cancer Biology 2020Quote: ... Total RNA was isolated from sorted 2-3×10^5 CD34+ cells using the mirVANA miRNA isolation kit (Thermo Fisher) and subsequently processed using the Small RNA Library Prep kit (Norgen Biotek) ...
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bioRxiv - Cancer Biology 2020Quote: ... 95% and 100% 2x) and in two changes of xylene 5 minutes each and finally mounted using xylene-based DPX mounting media (ThermoFisher). Slides were analysed using optical microscopy ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific knockdown was achieved by reverse-transfection of PCa cell suspensions (total 5×105 cells) with 12.5 nM siRNA in 6 well plates using RNAiMAX transfection reagent (Life Technologies; Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... and synovial sarcoma cell line (HS-SYII) were cultured in 5% CO2 in a humidified atmosphere at 37 °C in Dulbecco’s modified medium (DMEM) (Thermo Fisher) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... so that 50 μg of total protein could be mixed with 5% Coomassie brilliant blue G-250 before loading on a 3-12% Bis-Tris BNE gel (Invitrogen). After electrophoresis (2.5 hr ...
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bioRxiv - Cell Biology 2021Quote: ... patens cDNA using a 5’ CACC sequence on the specific primers for oriented cloning into the pENTR-D-TOPO vector (Invitrogen). LR clonase (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were then washed twice with ice-cold PBS and stained nuclei with Hoechst 33342 (5 µg/mL, Invitrogen) for 10 min ...
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bioRxiv - Cell Biology 2021Quote: Full length human NHSL1d cDNA including exon 1d and excluding exon 5 was generated by cloning overlapping EST clones Flj35425 and DKFZp686P1949 into pENTR3C (Invitrogen). Fragments of NHSL1 (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed three times with imaging buffer and incubated in imaging buffer containing EGTA-AM (5 μM, Invitrogen #E1219). After 45 minutes incubation ...
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bioRxiv - Biophysics 2020Quote: ... for 5 min at room temperature and washed twice with phosphate buffered solution (1×, pH 7.4, Gibco, Thermo Fisher Scientific). The microchips were then coated with fibronectin (15 μg/mL ...
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bioRxiv - Biophysics 2020Quote: ... for 5 min at room temperature and washed twice with phosphate buffered solution (1×, pH 7.4, Gibco, Thermo Fisher Scientific). The microchips were then coated with fibronectin (15 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was carried out in technical duplicates in 384-well plates on a QuantStudio 5 instrument (Thermo Fisher Scientific) using 2x Perfecta ROX,UNG Fast Mix (Quanta Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... HA-AP2M1 and eGFP were co-transfected at a plasmid ratio of 10:10:1 into neurons at DIV 5 using Lipofectamine 2000 reagent (Invitrogen). At 48 hours post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... the specimens were washed 2 × 5 min in PBS and mounted in ProLong® Gold antifade reagent (Life Technologies, P36934). Antibodies used for immunohistochemistry and their working concentrations are listed in Table 1.
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bioRxiv - Cell Biology 2020Quote: ... 5-ethynyl-2-deoxyuridine (EdU) labelling performed with Invitrogen Click-iT EdU Alexa Fluor 594 Imaging Kit (Life Technologies, C10339).
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bioRxiv - Biophysics 2020Quote: ... Nile Red (AC415711000), DiI-C18(5) (D3911), carboxylate-modified microspheres (0.1 μm, 350/440 blue fluorescent, F8797) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... slides were rinsed in PBS for 3 x 5 mins and stained with 1 %g/mL 4,6-diamino-2-phenylindole (DAPI - Molecular Probes) for 3 mins in the dark at RT ...