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Citations for Thermo Fisher :
9501 - 9550 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... After dispensing 5 μL of these master mixes to each well of a 384 well plate (Applied Biosystems PN 4309849) using a Mantis Liquid Handler (Formulatrix) ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were blocked in 1% BSA and then stained with indicated antibodies against Claudin-5 (Invitrogen 35-2500 1:50), Ad5 (Abcam ab6982 1:100) ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed three times in 1x PBS for 5 min and incubated with secondary antibodies [donkey anti-rabbit Alexa594 (1:1000, #A21207, Invitrogen) and goat anti-mouse Alexa488 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... were transformed into DH10EmBacY chemically competent cells with heat shock at 42°C for 15 seconds followed by incubation at 37°C for 5 hours in S.O.C media (Thermo Fisher Scientific). The cells were then plated on LB-agar plates containing kanamycin (50 μg/ml) ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 hours at RT and were exposed to ECL imager after 5 min incubation with ECL reagent (Thermo Fisher).
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bioRxiv - Cell Biology 2021Quote: ... 2X SSC) for 5 min and counter stained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies). Coverslips were mounted in imaging buffer (3.7 μg/μl glucose oxidase and 1U catalase in equilibration buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were washed with 1X PBS 3 times for 5 minutes and then mounted with Prolong Glass Antifade mountant (Invitrogen). Confocal Z-stack images were acquired using Nikon SoRA spinning disk microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... FBS was dialyzed in-house (against 0.15M NaCl until glucose reached <5 mg/dL) using 10k MWCO dialysis tubing (Fisher Scientific) at 4°C for 6 h ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were pelleted by centrifugation at 300RCF for 5 min and re-suspended at a density of 6,000 cells/µl in warm Neurobasal-A (Gibco #10888022) + 1X B27 supplement (Gibco #17504044 ...
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bioRxiv - Cancer Biology 2020Quote: Cells were sorted into 96-well plates at 50 cells/well in 5 µL CellsDirect™ 2× Reaction Buffer (Invitrogen). Cells lysates were reverse transcribed using Superscript™ III (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... Wells were consecutively washed two times for 5 min in washing buffer before incubation with secondary antibodies (Alexa Fluor-488 goat anti-mouse IgG, Invitrogen, A-11029 ...
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bioRxiv - Cell Biology 2021Quote: ... Passages were made before reaching confluence every 2-3 days by two washes with PBS (15 min each) and incubation in trypsin 0.05% for 5-10 min (Trypsin Cat#15400054, Thermo Fisher). MDCK cells stable transfected clones (MDCK-Shh ...
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bioRxiv - Cell Biology 2021Quote: ... we washed the chambers three times for 5 minutes in PBS and mounted them in ProLong Diamond Antifade Mountant (ThermoFisher). Images were acquired using a Leica DMI6000 inverted microscope with an integrated confocal module SP5 (Leica Microsystems ...
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bioRxiv - Cell Biology 2021Quote: ... with 5% CO2 at 37°C and propagated in the same media containing penicillin-streptomycin-glutamine (1:100 dilution of Gibco Penicillin-Streptomycin-Glutamine 100X ...
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bioRxiv - Neuroscience 2020Quote: ... recombinant human epidermal growth factor (5 ng/ml) and additionally supplemented with freshly added recombinant human fibroblast growth factor-basic (1 ng/ml; Gibco). hCMEC/D3 cells used for the experiments were between passages 6 and 11 ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was then transferred to 5 mL pre-warmed (20 min at 37°C) Trypsin/EDTA (Life Technologies, USA) supplemented with 132 mM trehalose (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: MCF10A cells were obtained from ATCC and cultured in DMEM/F-12 media with 5% horse serum (Thermo Fisher Scientific), 20 ng/ml human EGF (Peprotech) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells selected with CD11b microbeads were plated onto poly-L-lysine precoated 96-well or 386-well plates at 3x104 cell/cm2 density and were cultured at 37 °C in a 95% air/5% CO2 incubator in DMEM/Glutamax medium (#31966-021, Gibco) supplemented with 10% FBS (fetal bovine serum ...
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bioRxiv - Plant Biology 2021Quote: ... pistils and young seeds (7 DAP from CT and 5 DAP from HT) were isolated following a protocol for Trizol (Invitrogen). RNA from 26 DAP seeds was isolated using a NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5 mM EDTA and 0.5 % NP40) complemented with 1 × protease inhibitors (Pierce Protease Inhibitor Mini Tablets, EDTA-Free, Thermo Scientific) and 2 U Turbo DNase (Ambion ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μm) and a C18 analytical column (PepMapAcclaim C18, 50 cm × 0.75 mm, 2 μm, both Dionex-Thermo Fisher Scientific), applying a linear gradient from 2 to 35% solvent B (80% acetonitrile ...
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bioRxiv - Biochemistry 2020Quote: ... 4390771 ID: s69588 and s69586) or ERK3 siRNA (5 pmol; catalog number: 4390771 ID: s78451) and Lipofectamine 2000 (2 µl) (Invitrogen) and cells incubated for 19 h ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI was used as a counterstain at 300nM in 1xPBS for 5 minutes at room temperature and then mounted in mounting medium ProLong Diamond Antifade (ThermoFisher). Slides were imaged on a Keyence BZ-X810 microscope using the 10X objective.
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bioRxiv - Biochemistry 2020Quote: ... 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific, 43028) and quenching with 1N HCl ...
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bioRxiv - Cell Biology 2021Quote: ... 5–12 µl of protein lysate was loaded into pre-cast protein gels (4–12 % Bolt Bis Tris Plus, ThermoFisher) together with 10 µl of the molecular marker (PageRuler prestained ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Cell Biology 2021Quote: ... Human islets were loaded with 5 µg/µL of the Ca2+-sensitive dye Fluo-4 (1923626, Invitrogen, Thermo Fisher Scientific) for 60 min before being transferred to a recording chamber ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured at 37 C in 5% CO2 in growth media consisting of high-glucose DMEM (Gibco No. 10566), 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... sections were washed at least 3-5 times with TBSTX buffer and incubated with the appropriate Alexa fluorescent secondary antibodies (Invitrogen). Sections were washed 3-5 times with TBSTX buffer and then mounted on Superfrost slides (Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted down at 2,500 rpm for 3 mins and were washed 5 times with ice-cold DPBS (Gibco #14200075). The pellets were finally dissolved in DPBS and were counted by haemocytometer using Trypan Blue stain (Gibco #15250061) ...
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bioRxiv - Cell Biology 2021Quote: ... were triple-plated in 12-well plates at a concentration of 5×104 cells/well and cultured with osteogenic differentiation medium (Gibco; StemPro ® Osteogenesis Differentiation Kit) ...
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bioRxiv - Cell Biology 2021Quote: ... were triple-plated in 12-well plates at a concentration of 5×104 cells/well and cultured with adipogenic differentiation medium (Gibco; StemPro ® Adipogenesis Differentiation Kit) ...
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bioRxiv - Microbiology 2020Quote: Purified RNA of 10 μg was denatured at 95°C for 5 min in gel loading buffer II (#AM8547, Invitrogen) and separated by 6% polyacrylamide gel with 7M urea at 300 V for 2 h ...
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bioRxiv - Microbiology 2020Quote: ... the extracted RNA (5 μL) from each tissue of individual cases was reverse transcribed using the SuperScript IV VILO cDNA synthesis system (Invitrogen). The cDNA generated from each sampled tissue (2 μL ...
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bioRxiv - Systems Biology 2021Quote: ... against Tfap4 or a negative control siPOOL at a final concentration of 5 nM using the Lipofectamine RNAiMax transfection reagent (Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... denatured at 65°C for 5 min and treated with 10 U RNase-free DNase I (ThermoFisher Scientific, Waltham, USA) in DNase 1 buffer at 37 °C for 40 min ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 5% foetal calf serum (FCS) and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). KC cells (A Culicoides insect derived cell line ...
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bioRxiv - Microbiology 2020Quote: ... Aag2 cells were grown at 30 °C with 5% CO2 in DMEM high glucose media supplemented with 10% heat-inactivated fetal bovine serum (Gibco), 1% penicillin-streptomycin ...
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bioRxiv - Synthetic Biology 2021Quote: ... Annealing reaction was set up by mixing 1.5 µL of each oligonucleotide (100 fM) with 5 µL T4-DNA ligase buffer (Thermo Scientific) in a total reaction volume of 50 µL ...
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bioRxiv - Systems Biology 2021Quote: ... and samples were centrifuged at 16,000g for 5 minutes at −4°C in a Sorvall Legend Micro 17R microcentrifuge (Thermo Scientific). The supernatant (extract ...
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bioRxiv - Systems Biology 2021Quote: Metabolites were detected and quantified as area under the curve based on retention time and accurate mass (≤ 5 ppm) using the TraceFinder 3.3 (Thermo Scientific) software ...
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bioRxiv - Systems Biology 2021Quote: ... were cultured in a humidified incubator with 5% CO2 at 37°C in R10% medium: RPMI-1640 Medium (with glutamine, no phenol red, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2020Quote: ... The samples were then further post-fixed with 1.5 mM BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate) (Thermo Scientific A35396) in 1× PBS at room temperature for 20 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... were cultured at 37°C in an atmosphere of 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... the cultured cells were diluted 1:500 by adding 20 μL of the cell suspension to a flask containing 10 mL RNased NB and 10 μL 5-EU (100 mM stock solution; Invitrogen), giving a final concentration of 100 μM 5-EU ...
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bioRxiv - Plant Biology 2021Quote: ... the putative target of miR775 was validated by modified 5′RLM-RACE using First Choice RLM-RACE Kit (Ambion,USA) following manufacturer's instructions with minor modifications ...
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bioRxiv - Microbiology 2020Quote: RACE-PCR to determine the transcriptional start site was performed with the 5’ RACE System for Rapid Amplification of cDNA Ends (ThermoFisher) following the manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: ... amoebae transfected with vector control (pEhEx) were washed and resuspended in M199s medium and labeled with CellTracker green 5-chloromethylfluorescein diacetate (CMFDA; Invitrogen) at 186 ng/ml for 10 minutes at 35°C ...
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bioRxiv - Microbiology 2021Quote: ... Both HEK293T and A549 cell lines were maintained at 37°C and 5% CO2 in high-glucose DMEM (11-965-092, Gibco), supplemented with 10% fetal bovine serum (Genclone ...