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Citations for Thermo Fisher :
9601 - 9650 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: RPE1 (ATCC) cells were incubated at 37°C in 5% CO2 in high glucose Dulbecco’s modified eagle media (DMEM) (Life Technologies), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2020Quote: ... The gel was run following manufacturer instructions with “light blue” cathode buffer (0.5% NativePAGE™ Cathode Buffer Additive 20x, 5% NativePAGE™ Running Buffer 20x, 94.5% H2O) (Invitrogen) at 150 V followed by Coomassie R-250 staining ...
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bioRxiv - Biophysics 2020Quote: ... Cells were grown in an incubation chamber providing 37 °C and 5% CO2 in growth medium (DMEM, catalog no. 11880-02, Gibco) containing 1× MEM NEAA (catalog no ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated with spectral DAPI for 5 minutes and mounted with ProLong Gold antifade reagent (Life Technologies, cat #P36980). Negative controls included incubation with PBS solution instead of the primary antibody ...
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bioRxiv - Cancer Biology 2021Quote: PC-9 and HCC827 cell lines were cultured at 37C and 5% CO2 in RPMI 1640 GlutaMAX media (Gibco, US) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were trypsinized and treated 30 minutes at 37°C with 5 µM CellROX Deep Red reagent (Life technologies, C10422) diluted in medium ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were permeabilised in 0.2% triton for 5 min and incubated with Click-it reaction cocktail (Click-it Alexa Fluor 647 C-10424 Invitrogen) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated at 37 °C with 5% CO2 with Alexa Fluor 488–conjugated transferrin (25 μg/mL, ThermoFisher Scientific) for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and suspension diluted 1/5 by adding FACS staining buffer (SB; 2% heat-inactivated Fetal bovine serum (Life Technologies; 26140079), 25 mM HEPES (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... 5% CO2 in minimal medium containing 1 part DMEM (4.5 g/L D-Glucose, Pyruvate, L-Glutamine; Invitrogen 11971-025): 1 part DMEM/F12 ...
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bioRxiv - Cell Biology 2020Quote: ... HEK 293T cells were seeded at 5×10^6 cells and grown to 70-90% confluence in 10 cm plates in DMEM (Gibco) with 10% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... slides were washed 3 times in PBST for 5 minutes each and mounted using ProLong Diamond Antifade Mountant (Invitrogen, P3696). Coverslips were carefully placed over the sections ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in a final volume of 500 μl of 5% FBS in PBS and subsequently filtered into polypropylene FACS tubes (ThermoFisher). For scRNA-Seq experiments ...
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bioRxiv - Cell Biology 2020Quote: ... CD140a-PE (10 μl/106 cells, 558002, BDBioscience, Eysins, Switzerland) and CD140b-APC (5 μl/106 cells, A15719, Thermo Fisher). Right before analysis ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were loaded onto a trap column trapping cartridge (Acclaim PepMap C18 100Å, 5 mm × 300 μm i.d., #160454, Thermo Scientific) and separated by reversed-phase chromatography on a 50 cm μPAC C18 column (PharmaFluidics ...
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bioRxiv - Cell Biology 2021Quote: ... neurons were cultured at 37 °C with 5% CO2 and maintained in either maintenance media (Neurobasal (Gibco®, 21103-049) supplemented with 33 mM glucose (Corning ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 was cultured in RPMI-1640 medium supplemented with 10% heat-inactivated FBS and 5% Penstrep (Invitrogen and Himedia) in an incubator fixed at 37°C and equipped with 5% CO2 supply ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µg of plasmid was used to transfect 400,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 µL; Thermo Fisher) in OptiMEM (400 µL ...
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bioRxiv - Cell Biology 2021Quote: ... samples were boiled for 5 minutes in NuPage LDS sample buffer and run on NuPage Bis-Tris 4-12% protein gels (Invitrogen), and proteins were transferred to PVDF membranes (Immobilon-FL ...
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bioRxiv - Cell Biology 2021Quote: ... The retinal sections were rehydrated with PBS and blocked with PBS containing 5% (v/v) goat serum (Thermo Fisher Scientific) and 0.1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2020Quote: ... Labeled probes were denatured by heat for 5 min at 90 °C and then incubated with the blot in ULTRAhyp Ultrasensitive Hybridization Buffer (Invitrogen) overnight at 42 °C ...
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bioRxiv - Plant Biology 2020Quote: ... anthers were stained for a week at room temperature with 1 mM Calcofluor white for imaging of cell walls and 5 μM Syto13 (ThermoFisher) for imaging nuclei ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cross-linked for 15 minutes at room temperature with 1% formaldehyde and quenched for 5 mins at room temperature with 0.2 M glycine (Thermo Fisher). The cross-linked cells were aliquot (~ 3X106 cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was measured in technical and biological triplicate on a QuantStudio 5 Real-Time PCR machine (Thermo Fisher Scientific). Manual inspection of melting curves was performed to confirm PCR specificity ...
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bioRxiv - Cell Biology 2021Quote: ... Crypt fractions were centrifuged at 300g for 5 minutes and resuspended with ice-cold basal culture medium (Advanced DMEM/F12 (Gibco) supplemented with 2 mM GlutaMax (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... Different concentrations of acrylamide and bis-acrylamide were mixed to produce gels of different rigidity 5 and mixed together with 2% v/v fluorescent carboxylated 200 nm beads (Invitrogen), 0.05% APS (A3678 ...
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bioRxiv - Biophysics 2022Quote: ... The cells were cultured in a humidified incubator at 37C in 5% CO2 and 95% O2 using a high glucose DMEM (ThermoFisher) containing 10% fetal bovine serum (Life Technology ...
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bioRxiv - Cell Biology 2022Quote: ... Each sample was centrifuged at 200 × g for 5 min and pellets were incubated with 40 nM DiOC6 (D273, Invitrogen) for 15 min at 37°C and 5% CO2 to assess mitochondrial membrane potential ...
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bioRxiv - Biophysics 2022Quote: ... Matthias Mack (Signoret et al., 2000)MC-5 was fluorescently labeled using DyLight 650 NHS ester coupling kit (Thermo Fisher) with a dye:protein coupling ratio of 1.57:1.
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bioRxiv - Neuroscience 2022Quote: ... at a thickness of 300μM as before93,94 and placed in prewarmed aCSF with 5% CO2 and 95% O2 for 30m before transferring to Neurobasal Medium (ThermoFisher). They were treated with vehicle or 500μM glutamate for 3h at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were grown at 5% CO2 and 37 °C in DMEM high glucose media supplemented with 10% heat inactivated fetal bovine serum (FBS) (Gibco), penicillin/streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed 3 x 5 min with PBS before adding secondary antibody (1:200 Alexafluor-488 goat anti-mouse, Invitrogen) and rhodamine phalloidin (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... NC) or plasmid with HAX1 gene with tag on 3’end or 5’end of the gene (LipofectamineTM2000, ThermoFisher Scientific). Cells were detached and seeded on 100mm plates to grow single colonies (selection ...
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bioRxiv - Immunology 2022Quote: Two-millimeter tissue sections were excised 5 cm from the distal end of the small intestine (ileum) and stored in RNAlater (ThermoFisher) overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: ... 50 μg of proteins of tissue lysates were boiled for 5 min at 95°C in NuPAGE LDS sample buffer containing 1x reducing agent (Invitrogen). Lysates were separated by SDS–PAGE using the XCell SureLock Electrophoresis Cell on NuPAGE Novex 4%–12% Bis-Tris Protein Gels (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... DAPI was used as a counterstain at 300nM in 1xPBS for 5 minutes at room temperature and then mounted in mounting medium ProLong Diamond Antifade (ThermoFisher). Imaging was done at room temperature on a Nikon Eclipse Ti microscope with an UltraView VoX imaging system (PerkinElmer ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 x 0.075 mm, 2 μm particle size (Thermo Scientific), coupled to a Q Exactive HF-X Orbitrap (with HCD ...
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bioRxiv - Biochemistry 2022Quote: ... HDLM-2 and K562 cells were obtained from the AstraZeneca cell bank (Alderley Park, UK) and maintained at 37°C in 5% CO2 and grown in DMEM or RPMI-40 (Gibco) with 10% fetal bovine serum (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... stably expressing GFP-Tubulin obtained from Franck Perez lab were grown at 37°C and 5% CO2 in DMEM/F12 (31331028, Gibco) supplemented with 10% fetal bovine serum (10270106 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was decanted and incubated with a 5 mL 50% slurry of NiNTA (Thermofisher Scientific, Saint-Laurant, QC, Canada) resin for one hour with constant inversion ...
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bioRxiv - Bioengineering 2022Quote: ... 721.221 cell lines were kept in a humidified incubator at 37°C with 5% CO2 and cultured in complete RPMI1640 medium supplemented with 2 mM GlutaMAX (Invitrogen), 10% FBS (Sigma),10 mM HEPES pH 8.0 (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell line were maintained at 37°C and 5% CO2 in the presence of 100 unit/ml penicillin and 100 μg/ml streptomycin (Gibco) and were routinely tested for mycoplasma using MycoAlert mycoplasma detection kit (Lonza) ...
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bioRxiv - Biochemistry 2022Quote: ... the gel was rinsed in Milli-Q water for 5 minutes and stained for 20 minutes with 1x SYBR gold nucleic acid stain (Invitrogen) in 1x TBE ...
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bioRxiv - Neuroscience 2022Quote: ... were cultured at 37°C and 5% CO2 in high glucose Dulbecco’s Modified Eagle Medium with GlutaMAX™ Supplement (DMEM; Gibco) supplemented with 1 mM sodium pyruvate (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... were pulsed for 1 hr with 10μg/ml influenza virus NP(366-374) peptide and were subsequently labeled for 10 min at 37°C with 5 μM CFSE (Invitrogen) (CFSEhigh ...
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bioRxiv - Cancer Biology 2022Quote: ... All cells were maintained at 37°C and 5% CO2 in high humidity and cultured in high glucose DMEM (Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2022Quote: ... were used at a final concentration of 5 nM and mixed with 1.3 µl of Lipofectamine RNAiMAX (Thermo Fisher Scientific). The transfection mix was pre-spotted onto 24-well plates for 30 min at RT prior the addition of 7.0x104 A549 cells per well ...
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bioRxiv - Molecular Biology 2022Quote: ... Paraffin-embedded human tissues and hind paws were cut into 5 μm sections with the Microtome HM355S (Thermo Fisher Scientific) and transferred onto microscope slides.
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were rinsed 3 times for 5 minute intervals with PBS and mounted on microscope slides (Fisher Scientific, 22-178277) using ProLong Glass Antifade Mountant (Thermo Fisher Scientific ...