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Citations for Thermo Fisher :
9801 - 9850 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Wild type wis1+ open reading frame or versions in which Methionine at 395 was substituted with alanine or glycine were synthesised by integrated DNA technologies (IDT) with PstI site at 5’end and KpnI site at 3’end and cloned into pJet1.2 (Thermo Scientific). Wild type wis1+ ORF was amplified by Phusion PCR from NT4 genomic DNA with primers containing PstI and KpnI restriction sites at 5’ end and 3’ end respectively ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with scrambled small interfering RNA or small interfering RNA (siRNA) to silence TMX4 expression (5′-GCAUGGUGUUCUUACGUUUtt-3′, 100 nM per dish, Silencer Select Pre-designed, Ambion). Cells were processed for immunofluorescence or for biochemical analyses after 48h of transfection.
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bioRxiv - Physiology 2022Quote: ... then perfused the lungs with 3 mL of ice-cold DPBS containing Ca2+ and Mg2+ followed by 5 mL of 4% methanol-free formaldehyde (ThermoFisher) in DPBS containing Ca2+ and Mg2+ ...
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bioRxiv - Physiology 2022Quote: ... 1×105 NRK-49F cells were cultured under 37°C and 5% CO2 in plastic culture plates with Dulbecco’s Modified Eagle Medium (DMEM-Low glucose, Invitrogen, USA) containing 5% inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... cells were resuspended in neuronal plating medium (NPM: Minimal Essential Medium with Earl’s Salt and L-glutamine, 0.6% D-glucose, 5% fetal bovine serum; Invitrogen, Carlsbad, USA) and seeded to a concentration of 3.0 × 105 cells per well on EM grids and glass bottom dishes (MatTek corp. ...
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bioRxiv - Cell Biology 2019Quote: The pellets (n = 5/time point/group) were rinsed in PBS and lysed in 500 μl TRIzol (Invitrogen, CA, USA) for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... and MIA PaCa-2 cell lines were a gift from the laboratory of Ben Stanger (University of Pennsylvania) and cultured in DMEM (Mediatech) with 5% FBS (Invitrogen) and 1% antibiotic-antimycotic (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: Caco-2 cells were incubated on sterile coverslips at 37°C in 5% CO2 with 3.5 or 14 μg of PKH26-labeled protozoal EVs (ThermoFisher™) for 1h ...
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bioRxiv - Microbiology 2019Quote: ... The first-strand cDNA was reverse transcribed from 5 μg of total RNA with the SuperScript IV First-Strand Synthesis System (Invitrogen). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Systems Biology 2019Quote: ... Cell lines were grown at 37°C with 5% CO2 in humidified tissue culture incubators (Thermo Scientific, Waltham, MA, USA).
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bioRxiv - Systems Biology 2019Quote: ... 4 µL of medium containing one of 1,833 different bioactive library compounds was then added (final concentration 5 µM) using a Versette robotic liquid handler (ThermoFisher Scientific) and plates were immediately transferred to an IncuCyte Zoom dual color live content imaging system (Model 4459 ...
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bioRxiv - Bioengineering 2019Quote: ... FDB fibers are stained at room temperature for 20 min prior to imaging with 5 µM of the calcium-sensitive dye mag-fluo-4 AM (KD ~22 µM, Invitrogen) dissolved in Tyrode’s buffer.
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bioRxiv - Neuroscience 2019Quote: ... The non-specific binding of antibodies was avoided by incubating the cells with 5% normal goat serum(Thermo Fisher Scientific)/ 0.4% Triton X-100(Sigma)/1% Glycine(Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... and 105 μg AAV-PHP.eB were mixed with 5 mL of Opti-MEM I media (Reduced Serum, GlutaMAX; ThermoFisher Scientific) and 1.1 mL of a solution of 1 mg/mL 25 kDa linear Polyethylenimine (Polysciences ...
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bioRxiv - Immunology 2019Quote: 10% highest and lowest Nur77-eGFP sorted CD4+CD25-CD62LhiCD44lo naïve T cells from SKGNur and WTNur LNs and spleen were labeled with 5 μM Cell Trace Violet according to the manufacturer’s instructions (Life Technologies) before culturing in a 1:10 ratio with 5×105 BALB/c splenic APCs (prepared via Thy1.2+ cell depletion by MACS ...
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bioRxiv - Immunology 2019Quote: 10% highest and lowest Nur77-eGFP sorted CD4+CD25- CD62LhiCD44lo naïve T cells from SKGNur and WTNur LNs and spleen were labeled with 5 μM Cell Trace Violet according to the manufacturer’s instructions (Life Technologies) before culturing with plate-bound αCD3 at the indicated concentration and 2 ug/mL αCD28 in a 96-well flat-bottom plate in complete RPMI medium for 3 days ...
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bioRxiv - Plant Biology 2019Quote: ... and then rotated for another 5 min before loading the strip into the well of a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen).
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... cells were centrifuged for 5 min at 1400 rpm and incubated at 37°C for 2 min in sterile RBC lysis solution (Invitrogen). Lysis was arrested by adding 30 ml PBS ...
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bioRxiv - Pathology 2019Quote: ... Aliquot concentration was determined by enumerating colony growth on agar plates with 5% sheep blood (Remel, Thermo Fisher, Lenexa, KS) at the time of freezing ...
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bioRxiv - Cell Biology 2019Quote: ... Ecadherin-GFP cells and α-catenin KD MDCK cells were maintained in media containing 5 μg/ml geneticin (Life Technologies).
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bioRxiv - Systems Biology 2019Quote: Jurkat T cells (ATTC TIB-152) and K562 cells (ATCC CCL-243) were maintained in 5% CO2 environment in RPMI (GIBCO) media supplemented with 10% fetal bovine serum ...
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bioRxiv - Biophysics 2019Quote: QDs were functionalized with benzyl guanine by mixing 5 μl of 8 μM amino (PEG) QDs emitting at 655 nm (ThermoFisher) with 2 μl of 20 mM BG-GLA-NHS (NEB ...
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bioRxiv - Systems Biology 2019Quote: Metabolites were detected and quantified as area under the curve based on retention time and accurate mass (≤ 5 ppm) using the TraceFinder 3.3 (Thermo Scientific) software ...
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bioRxiv - Molecular Biology 2019Quote: ... At least 5 µg of mRNA was purified from total RNA using the Poly(A)Purist MAG kit (AM1922, Ambion). mRNAs were fragmented for 3 minutes at 70 °C using a Zinc-based alkaline fragmentation reagent (AM8740 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We used 300 ng of purified PCR product to set a BPII recombination reaction (5 μL) into the Gateway Entry Vector pDONR201 (150 ng) according to the manufacturer’s instructions (Invitrogen, USA). BpII reaction mix was incubated overnight at 25°C ...
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bioRxiv - Cell Biology 2020Quote: ... pCR2-TUB1 consists of the region of the TUB1 locus from the intron (situated close the 5’ end of the gene) to 385 bp after the stop codon cloned into the pCR2 vector (Invitrogen). The HIS3 gene expression cassette was ligated into the BsrGI site within the 3’ untranslated region of the TUB1 sequence within pCR2 (pCR2-TUB1) ...
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bioRxiv - Immunology 2019Quote: ... The suspension was shaken for 10 s before addition of 5 ml of L-15 medium supplemented with 10% foetal calf serum (Invitrogen) and centrifugation at 400 g for 5 min ...
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bioRxiv - Synthetic Biology 2019Quote: ... Forward and reverse primers were added to a final total primer concentration of 0.1 μM in addition to 5 μL of Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher). Nuclease-free water was added for a final reaction volume of 10 μL ...
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bioRxiv - Developmental Biology 2020Quote: ... for 3 to 5 min at 37°C and resuspended in differentiation medium N2B27 (Advanced DMEM F12, Neurobasal vol:vol (Life Technologies)) ...
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bioRxiv - Biophysics 2020Quote: ... cells grown in glass-bottom dishes in FBS-containing medium were stained with 5 μM laurdan (Molecular Probes, Eugene, OR) for 5 min ...
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bioRxiv - Cell Biology 2019Quote: Cells expressing G12V Rac1A under a tetracycline-inducible promoter along with GFP-WASP or RFP-tagged active Rac marker were resuspended in LoFlo and seeded to a final concentration of 5×105 cells/ml on a borosilicate glass 8 well chamber (ThermoFisher) or on a 35 mm glass bottom dish (MatTek) ...
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bioRxiv - Microbiology 2019Quote: ... Blots were blocked and proved with 1° antibody in 5% milk (vol/vol) in 50 mM Tris-buffered saline with 0.05% (vol/vol) Tween-20 (Fisher Scientific). Monoclonal antibodies against Mycobacterium tuberculosis GlcB (Gene Rv1837c) ...
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bioRxiv - Bioengineering 2019Quote: ... The pre-PCR amplification reaction was performed in a 5 µl reaction mixture containing 2.5 µl TaqMan PreAmp Master Mix 2X (Applied Biosystems), 1.25 µl of 96-pooled LNA™ assay mix (0.25X ...
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bioRxiv - Microbiology 2019Quote: ... CD14-positive monocytes were seeded into 12 well plates at 5 × 105 cells/well and differentiated into macrophages by culture in complete RPMI 1640 medium (Gibco) supplemented with 10% heat-inactivated human AB+ serum ...
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bioRxiv - Neuroscience 2019Quote: ... and then transfected with 2.5 µg of donor DNA and 1.25 µg of each targeting construct (Supplemental Table 3) using Lipofectamine Stem (Invitrogen STEM00003) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Transferred RNA was UV cross-linked and hybridized overnight with 100 ng/mL of 5’-biotinylated probes (Table S2) in ULTRAhyb hybridization buffer (Ambion) at 42 °C as described previously (27 ...
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bioRxiv - Microbiology 2019Quote: ... Beads were then washed with ice-cold lysis buffer 3 times for 5 minutes and proteins were recovered by boiling in denaturing loading buffer (Invitrogen).
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bioRxiv - Neuroscience 2020Quote: ... Briefly, cDNA was derived from 5 week-old C57BL/6J retina (Jackson, 000664) following RNA extraction (RNAqueous Kit, Ambion, AM1912) and reverse transcription with Superscript III (ThermoFisher ...
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bioRxiv - Genomics 2019Quote: ... and slides were rinsed in PBS for 5 minutes three times before a cover slip was applied using ProLong Gold Antifade Mountant (P10144, Invitrogen). 40x images representative of the sample were obtained by a blinded investigator using a Nikon Eclipse 80i with Ds-Ri2 camera and NIS Elements V4.3 software (Nikon Instruments ...
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bioRxiv - Cancer Biology 2019Quote: ... VA) and cultured at 37°C in a humidified 5% CO2 atmosphere in DMEM medium (Gibco; Thermo Fisher Scientific, Inc.) with 10% fetal calf serum (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: ... VA) and cultured at 37°C in a humidified 5% CO2 atmosphere in DMEM medium (Gibco; Thermo Fisher Scientific, Inc.) with 10% fetal calf serum (Gibco ...
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bioRxiv - Plant Biology 2019Quote: ... and appropriate buffer containing deoxynucleoside triphosphates (dNTPs) and MgCl2 in a final concentration of 5 mM (1×; Thermo Fisher Scientific), was incubated at 25°C for 10 min and at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... 100 ng of extracted vRNAs were reverse transcribed using a Uni-12 primer (5’-AGCRAAAGCAGG-3’) and Superscript III reverse transcriptase (Invitrogen). Quantification was performed by qPCR on a Rotor-Gene Q 2plex System (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... at a 1:10000 dilution in 5% skimmed milk-PBST and 1:20000 secondary HRP Rabbit-anti-mouse antibody (Invitrogen) in 5% skimmed milk-PBST16.
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bioRxiv - Developmental Biology 2019Quote: HEK293 cells were passaged in HyClone DMEM (GE) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco) at 37°C and 5% CO2 under humidified conditions ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were pelleted by centrifugation at 300RCF for 5 minutes and re-suspended at a density of 6,000 cells/µl in warm Neurobasal-A (Gibco #10888022) + 1X B27 supplement (Gibco #17504044 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were then permeabilized and blocked for 5 min in DPBS containing 0.2% saponin and 10% FBS (Thermo Fisher Scientific). Cells were stained with primary (1:25-50 ...
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bioRxiv - Synthetic Biology 2020Quote: Samples were run on 4% agarose (Bio-Lab, Israel) in 1× TBE buffer (Biological industries, Israel) supplemented with 5 mM MgCl2 (Ambion), and stained with 0.3 µg/ml ethidium bromide (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were harvested for flow cytometry using FACS buffer (PBS pH 7.4, 2–5 mM EDTA, 0.1% BSA) or using 0.05% Trypsin-EDTA (Thermo Fisher Scientific #25300120) for 5 min followed by quenching with medium ...