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Citations for Thermo Fisher :
9901 - 9950 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS and incubated for 1 h at 37°C and 5% CO2 with Opti-MEM (Gibco) containing the indicated inhibitors ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed once with PBS and incubated for 2 h at 37°C and 5% CO2 with Opti-MEM (Gibco) containing 5 or 2.5 μM remdesivir alone or in combination with 10 μM ramelteon (PubChem ID 208902) ...
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bioRxiv - Cell Biology 2020Quote: ... Nuclei are stained with DAPI for 5 min before mounting with ProLong™ Gold Antifade Mountant (Thermo Fisher Scientific, P36930).
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bioRxiv - Developmental Biology 2020Quote: Neural retinae were dissected from adult zebrafish dark adapted for 12 hours between 5:30 PM and 6:30 PM and stored in RNAlater (Ambion) at 4°C until extraction ...
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bioRxiv - Biochemistry 2020Quote: ... and an Acclaim™ Pepmap™ 100 C18 analytical column (length 15 cm, 5 μm particle size, Thermo Scientific, USA). 2 μL of the bacteriocin sample was loaded onto the trap column at 3% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... biotinylated amyloid peptides spanning the amyloid beta sequence (−5 to 45) were pre-incubated with NeutrAvidin (ThermoFisher Scientific, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: HeLa and RPE1 (ATCC) cells were incubated at 37°C in 5% CO2 in high glucose Dulbecco’s modified eagle media (DMEM) (Life Technologies), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2019Quote: ... with 10% fetal calf serum (FCS) at 37°C in an atmosphere with 5% CO2 and transfected with lipofectamine (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were washed in PBS (2×10 min at 1500 rpm) and RPMI 1640 + 10% fetal bovine serum (FBS) (5 min at 1500 rpm) (all products from ThermoFisher). Monocytes were isolated with anti-CD14 microbeads (Miltenyi ...
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bioRxiv - Genomics 2019Quote: ... Cells were resuspended in 1 ml IP lysis buffer [25 mM Tris-HCl pH 7.5 / 150 mM NaCl / 1% NP-40 / 1 mM EDTA / 5% glycerol / Halt protease inhibitor cocktail (Thermo scientific) / Halt phosphatase inhibitor cocktail (Thermo scientific)] and incubated on ice for 1 h ...
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bioRxiv - Plant Biology 2020Quote: Regions screened for transcription factor binding via Yeast One-Hybrid were first inserted into pENTR 5’TOPO TA entry vector (Thermofisher) and subsequently placed into the pMW2 and pMW3 destination vectors containing HIS3 and LACZ marker genes respectively (Deplancke et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... Input for each sample consisted of 500 ng of RNA and 5 μL of 1:500 diluted ERCC spike-in mix 2 (Ambion). Libraries were amplified for 11 cycles during the final amplification step ...
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bioRxiv - Synthetic Biology 2020Quote: ... The membrane was blocked with 5% skim milk in TBST and probed with HRP-conjugated anti-flag mouse monoclonal antibody (Thermofisher) diluted at 1:10000 in the blocking buffer ...
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bioRxiv - Bioengineering 2020Quote: ... the cells were stained for 5 minutes at room temperature with LIVE/DEAD Fixable Violet Dead Stain Kit (ThermoFisher L34864). Subsequently ...
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bioRxiv - Synthetic Biology 2020Quote: ... in 50 ml 1X TAE prepared in Milli-Q® water + 5 μl SYBR™ Safe DNA Gel Stain (Invitrogen™ ...
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bioRxiv - Cell Biology 2020Quote: ... The final concentration of the TMT labeled digest/peptide mixture was 0.33 μg/μl and 5 μl of this mixture was loaded onto a C18-reversed phase column (Thermo Scientific, Acclaim PepMap C18 column ...
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bioRxiv - Immunology 2020Quote: ... were added to 7.5□μL of a master mix containing 5□μL of Power SYBR green mix (4367659, Applied Biosystems) and 2.5□μL of nuclease-free water with nCoV_IP2 primers (nCoV_IP2-12669Fw ...
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... and mAb 18B7 (10 µg/ml) for 30 minutes followed by incubation with Alexa-Fluor 546 anti-mouse IgG1 (5 µg/ml; Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Biochemistry 2021Quote: To each well in the plate was added 25 ng of pMIR-B3GLCT plasmid in 5 μl Opti-MEM (Gibco) and 0.11 μl lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were maintained at 37°C in 5% CO2 in serum-free Neurobasal plus medium containing supplement B27 plus (Invitrogen) for up to 3 weeks ...
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bioRxiv - Neuroscience 2020Quote: ... 5% DTT) for 10 min at 70 °C before being loaded on a 4-12% Bis-tris gel (ThermoFisher NP0322BOX) at 10 µg/lane ...
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bioRxiv - Neuroscience 2020Quote: ... at 37°C and 5% CO2 in mitotically arrested Mouse Embryonic Fibroblasts (MEFs) conditioned media (DMEM/F12 (Life Technologies, #11330057), 20% Knock-out Serum Replacement (KSR ...
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bioRxiv - Developmental Biology 2021Quote: ... and the solution was denatured at 95°C for 5 min and run on a ABI3130XL Genetic Analyzer (Applied Biosystems), using GeneMapper software (Life Technologies).
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed 3x 5 minutes in PBST and then incubated for 2 hours at room temperature with Alexa-Fluor secondary antibodies (ThermoFisher) (all 1:1000 in PBST + 5% BSA + 0.02% azide) ...
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bioRxiv - Plant Biology 2020Quote: ... 60-mer DNA oligonucletoides were 5’ end-labeled using [γ-32P]-ATP and PNK (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were pre-treated with 5-ethynyl uridine (EU) for 30 min prior to nuclei fractionation using Sigma kit (#NUC101) followed by Invitrogen Click-iT™ Nascent RNA Capture Kit protocol for nascent transcript extraction (#C10365) ...
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bioRxiv - Genomics 2021Quote: ... American Type Culture Collection (ATCC)] were cultured at 37°C and 5% CO2-concentration in DMEM supplemented with 10% fetal bovine serum (Gibco Invitrogen), 100 units of potassium penicillin and 100 μg of streptomycin sulfate per 1 ml (Lonza) ...
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bioRxiv - Biochemistry 2020Quote: ... charged by Ni2+ ions or 1 ml Tricorn 5/10 column (Cytiva) packed by Ni-NTA resin (Thermo Fisher Scientific). Columns were equilibrated by the 50 mM Na-PO4 ...
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bioRxiv - Immunology 2021Quote: ... The 96-well plates w preloaded with 9 μL of RT-STA solution: 5 μL of CellsDirect PCR mix (Invitrogen), 0.2 μL of SuperScript-III RT/Platinum Taq mix (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... was added to the cells in half culturing volume (40 μL added) and incubated in the dark for 5 min prior to reading on a Varioskan LUX plate reader (ThermoFisher). Measurements were done on all ten mouse sera samples from each group in at least duplicate ...
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bioRxiv - Immunology 2020Quote: ... and plated in culture dishes (TPP) with 5 x 106 cells/dish in 12 mL of complete RPMI (RPMIc): RPMI (Gibco) with 10 % V/V Hi FCS (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... Mast cells and macrophages were obtained from the peritoneal cavity by lavage with 5 ml cold PBS + EDTA (2 mM, Gibco). Cells were resuspended in RPMI medium containing 10% FCS.
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked with 5% milk in PBS for 1 hour and incubated overnight with primary antibody diluted in PBS which contained 5% milk and 0.2% Tween 20 (BP337, Fisher Scientific). The following day the membrane was rinsed several times with TBST (tris-buffered saline with 0.1% Tween 20 ...
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bioRxiv - Microbiology 2021Quote: ... volvulus worm to each well of 6-well plates containing 5 ml of DMEM +10% fetal bovine serum (Gibco, A3840002) + 100 u/ml penicillin + 100 ug/ml streptomycin + sodium bicarbonate (2 g/1) ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were heated at 95°C for 5 min and separated on 4520% acrylamide Tris-Glycine gradient gels (Life Technologies). Proteins were transferred to polyvinylidene difluoride membranes (EMD Millipore ...
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bioRxiv - Developmental Biology 2019Quote: ... 50-100 μg of total protein extracts were solubilized in SDS-sample buffer by boiling at 95°C for 5 minutes and analysed by SDS polyacrylamide gel electrophoresis (4-12% NuPAGE gel; Invitrogen). Western blotting was performed using antibodies against Vasa (rat ...
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bioRxiv - Developmental Biology 2019Quote: ... isolated cardiomyocytes were concentrated by 20 × g centrifugation for 5 min and re-suspended in cell culture medium [DMEM (Gibco), 10% FBS ...
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bioRxiv - Biochemistry 2020Quote: ... T-rex 293 cells (approximately 3 × 106 cells per 10-cm dish) were transfected with 5-HT2C wild-type or mutant DNA using Lipofectamine 2000 (Invitrogen) following manufacturer’s protocols ...
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bioRxiv - Genomics 2019Quote: The flasks were incubated for five days at 37°C in 5% CO2 and RNA was isolated using Trizol (Ambion) according to the method described by the manufacturer ...
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bioRxiv - Molecular Biology 2019Quote: ... The percentage of CD4-expressing cells was measured after incubation for 10 minutes with 1 µl of anti-CD4 antibody coupled to Alexa 647 (rat isotype, RM4-5, Invitrogen). For each cell line ...
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bioRxiv - Biochemistry 2019Quote: ... blocked with 5% milk in TBST and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (ThermoFisher MA1-21315, 1:3000). Blots were incubated with primary antibodies overnight at 4 °C with rocking and were then washed (3×5min ...
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bioRxiv - Cell Biology 2019Quote: ... in which the HEPES was replaced with 25 mM sodium bicarbonate and the PVA was replaced with 5% fetal bovine serum (Invitrogen). Media were equilibrated with 5% CO2 and 95% air ...
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bioRxiv - Biochemistry 2019Quote: ... HRP-conjugated secondary antibodies were either inactivated by incubation with sodium azide in 5% skim milk/TBST or stripped with Restore Stripping Buffer (ThermoFisher). The following antibodies were used in this study ...
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bioRxiv - Microbiology 2019Quote: ... the mixture was heated to 65°C for 5 min using the 2720 Thermal Cycler (Applied Biosystems; Foster City, USA) and rapidly cooled on ice afterwards to prevent renaturation ...
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bioRxiv - Immunology 2019Quote: ... isolated lungs were cut into 2-3 mm2 sections and resuspended in 5 mL digestion solution of Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco) supplemented with 5 mM GlutaMax (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: ... centrifuged for 5 minutes at 400 × g and resuspended in B27 medium (Neurobasal supplemented with 1% B27 without vitamin A (Gibco), 2 mM L-glutamine and 0.2% penicillin/streptomycin) ...
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bioRxiv - Bioengineering 2019Quote: ... proteins were transferred onto a nitrocellulose membrane and then blocked overnight while shaken at 4°C in TBST-5% BSA – a TBS Tween-20 (TBST; ThermoFisher) solution containing 5% bovine serum albumin (BSA ...
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bioRxiv - Genetics 2019Quote: ... Primary blood lymphocytes were isolated after venipuncture into a Vacutainer CPT (Becton Dickinson, Franklin Lakes, NJ) per the manufacturer’s recommendations and grown in 5 mL RPMI 1640 (Life Technologies) supplemented with 10% fetal bovine serum (Hyclone ...
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bioRxiv - Physiology 2019Quote: ... Sulfhydrated proteins in the dried bands were reduced with 0.1 mL of 65 mM dithiothreitol (DTT) (Fisher Scientific # BP172-5) at room temperature for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: ... pelleted again by centrifugation and resuspended at 3−5 × 106 cells/ml in sterile PBS containing 1.25 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; ThermoFisher Scientific) and stored on ice until required ...