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Citations for Thermo Fisher :
9651 - 9700 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The protein complex was purified by a Superdex 200 16/60 column in a buffer containing 5 mM HEPES pH 7.5 (Life Technologies) and 150 mM NaCl (Quality Biological ...
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bioRxiv - Immunology 2022Quote: ... 0.1 mM EDTA) for 5 minutes at room temperature and then neutralized with B cell media (RPMI 1640 with L-glutamine (Gibco) supplemented with 15% fetal bovine serum (Corning) ...
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bioRxiv - Immunology 2022Quote: ROS production was assessed after 2.5 h of stimulation as previously described (59) with 5 μM of the CellROX™ green reagent (Thermofisher). Cells were acquired on an Attune NxT.
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bioRxiv - Neuroscience 2022Quote: Using a subcloned and sequenced PCR fragment (162 bp – Table S1) of the mouse 5-HT2AR cDNA (Zero Blunt TOPO PCR Cloning kit, Invitrogen), high specific-activity RNA probes were produced from a linearized plasmid (HindIII ...
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bioRxiv - Pathology 2022Quote: ... for 5 min at 95 °C before they were loaded in Bolt™ 4–12 % Bis-Tris gels (Thermo Fisher). After separation ...
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bioRxiv - Physiology 2022Quote: Mouse NIH3T3 cells were transfected with pExpress-LoxBsr-FLAG-2xNLS-EGFP or pExpress-LoxBsr-FLAG-mGCNA then later selected for resistance to Blasticidin (5 µg/ml, catalog no. A11139-03, Gibco). Resistant clones were then screened for the expression of FLAG-2xNLS-EGFP or FLAG-mGCNA by immunoblotting of FLAG (see later in “Western Blotting” part) ...
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bioRxiv - Biochemistry 2022Quote: ... A total of 405 WT peptides were identified (Table 4) on the Fusion Lumos OrbiTrap mass spectrometer (mass tolerance <5 ppm) using Proteome Discoverer 2.2.0 software (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... to final concentration 0.5 mg/ml protein and 6x dye and heated from 10 to 70 °C with fluorescence reading every 0.1 °C (4 to 5 sec) in a QuantStudioTM 7 Flex Real-Time PCR System (Life Technologies). Protein thermal melting curve were generated using the Protein Thermal Shift TM software ...
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bioRxiv - Developmental Biology 2022Quote: ... 5’L1-3’L2 orientation were inserted via recombination into a pCS2+ Gateway-converted vector (Custom vector conversion kit; Invitrogen). Details of the Gateway plasmid are available upon request ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were boiled at 95°C for 5 min and loaded on SDS-PAGE (NuPAGE 4-12% Bis-Tris, Invitrogen). Efficiency of ligand coupling to the Ad capsid (% total hexon protein coupled ...
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bioRxiv - Developmental Biology 2022Quote: ... F 5’-GAACTGTCCAGATGCCCTTCCAGTT-3’ and R 5’-GCATCTGGACAGTTCTGGGAAGCCCG-3’) was cloned by PCR and ligated into the pEF6/V5-His TOPO plasmid vector (Invitrogen). FBLN7-V5-His vector was transfected into CHO cells (FBLN7-CHO ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected with remaining adherent cells collected by 5 min incubation with Cell Dissociation Buffer (Gibco, Thermo Fisher Scientific) at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected with remaining adherent cells collected by 5 min incubation with Cell Dissociation Buffer (Gibco, Thermo Fisher Scientific) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... hiPSC-CMs were quickly washed with DPBS and incubated with SARS-CoV-2 at 5 multiplicity of infection (MOI) diluted in DMEM only (Gibco) for 1 h at 37 C ...
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bioRxiv - Microbiology 2022Quote: ... An equivalent of 0.25 midgut and 5 salivary glands were resolved in a NuPAGE™ 10% Bis-Tris Protein Gel (Invitrogen) under reducing conditions and transferred to a PVDF membrane Invitrogen™ Power Blotter Select Transfer Stacks ...
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bioRxiv - Cell Biology 2022Quote: Human embryonic kidney 293T (HEK 293T) cells (ATCC CRL-3216) were cultured at 37 °C and 5% CO2 in DMEM (Gibco) supplemented with 4.5 g/L glucose ...
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bioRxiv - Cell Biology 2022Quote: K-562 Chronic Myelogenous Leukemia cells (ATCC, CCL-243) were cultured at 37 °C and 5% CO2 in IMDM (Cat# 12440053, Gibco) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Immunology 2022Quote: ... complementary DNA (cDNA) was generated from extracted mouse plasma RNA using primer YB383 5’-TTTTTTTTTTTTTTTTTTTTTTTTRAAGCAC-3’ and enzyme Superscript III (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Those at the early headfold to early somite stages were selected and cultured by the roller culture method under 5% CO2 at 37°C in 50-ml tubes containing DMEM or FluoroBrite DMEM (A1896701, ThermoFisher) supplemented with 75% rat serum ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were passaged every 4-5 days using 0.5 mM EDTA (pH 8.0) (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2022Quote: ... The membranes were again washed 3× in PBS for 5 min each and mounted onto slides using ProLong Gold Antifade (Invitrogen). Images of stained mTECs were obtained using an SP8 (Leica Microsystems ...
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bioRxiv - Biochemistry 2022Quote: ... 550 pmol of protein was then added to a tube containing 1.1 nmol of 5’Biotin(GGTTAG)2 DNA (Thermo Fisher) in a volume of 100 µL dilution buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... CRL-3216) were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco; cat. no. 12800-017) supplemented with 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then washed 3x for 5 mins with 1xPBS before mounting onto glass slides using ProLong Diamond anti-fade mountant (Invitrogen) and left to dry for 24hrs before imaging ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples were heated at 95°C for 5 minutes before loading and separated onto SDS-PAGE cassette (Thermo Fisher Scientific Bolt™ 4-12% Bis-Tris Plus Gels ...
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bioRxiv - Cancer Biology 2022Quote: ... and 0.06% Bromophenol Blue) at 98°C for 5 minutes and separated on 12% sodium dodecyl sulfate (SDS)-polyacrylamide gels (Invitrogen). Proteins were transferred to nitrocellulose membranes (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The inoculum was removed and cells washed 2× with PBS and 500 μL culture media (high glucose DMEM + 5% FBS (Gibco) + 1% antibiotic-antimycotic ...
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bioRxiv - Neuroscience 2022Quote: ... jGCaMP7b-XC or jGCaMP7b-XN and 1 μg cDNA encoding CFP (for labelling the soma area and neurites) were transiently transfected into DIV 5-7 cultured cortical neurons by Lipofectamine 2000 (Invitrogen) with a typical protocol according to the manual ...
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bioRxiv - Developmental Biology 2022Quote: ... span down at 200x g for 5 min and resuspended in Aggregation media consisting of KnockOut DMEM/F12 (Gibco, 12660012), 20% KnockOut Serum Replacement (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... we performed 5-ethynyl-2′-deoxyuridine (EdU) staining using the Click-iT EdU Alexa Fluor 647 imaging kit (C10340, Invitrogen). Four-color image acquisition of three replicates per condition was performed on an SP8 confocal microscope (Leica) ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged at ~300g for 5 minutes at room temperature and washed twice in 5ml of prewarmed DPBS (Dulbecco’s phosphate-buffered saline) (Gibco: 14190250) and subsequently seeded at 0.25*106 cells/ml in IMDM ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed three times for 5 minutes with PBS and incubated with 4μg/mL of goat anti-rabbit-Alexa-488 (ThermoFisher Scientific) in the blocking solution for 30 minutes at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were dried for 30 mins in a 37°C incubator and washed 3×5 min with 0.1% TritonX-100 in PBS (PBST) and incubated in 10% Horse Serum (ThermoFisher, #26050070), 0.4% TritonX-100 in 1x PBS (blocking buffer ...
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bioRxiv - Biophysics 2022Quote: ... HEK-293 cells cultured in DMEM supplemented with 10% FBS at 37°C with 5% CO2 were co-transfected with hERG1a in pcDNA3 and GFP in pcDNA3 using lipofectamine 3000 (Invitrogen). Cells were plated onto coverslips 14–16 h after transfection and cells with green fluorescence were selected for recordings ...
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bioRxiv - Cancer Biology 2022Quote: The NCI-H460 cell line was obtained from the American Tissue Collection Center (ATCC) and cultivated in a humidified atmosphere at 37°C and 5% CO2 using RPMI medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... and samples were centrifuged at 8000xg for 5 minutes at −9°C in a Sorvall Legend Micro 17R (Thermo Scientific). The supernatant (extract ...
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bioRxiv - Bioengineering 2022Quote: ... and stable pAGDisplay-RBD cell lines were cultured at 37°C in a 5% CO2 atmosphere in DMEM medium (4.5 g/L glucose, L-glutamine; Gibco, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... The dissociated cells and tissue fragments were centrifuged at 1,000 rpm for 5 mins at 4 ℃ and cultured in a T75 tissue-culture flask in a growth medium (advanced DMEM/F12 (Gibco) containing 5% bovine serum (Gibco)) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were incubated in a blocking/permeabilization buffer (0.3% Triton X-100 and 5% normal goat serum (Fisher Scientific) in 1X PBS ...
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bioRxiv - Plant Biology 2022Quote: ... Rosettes were rinse 3 times in distilled water and stained 20 min in a 5% (v/v) Lugol’s iodine solution (Fisher Scientific). After 3 rinses in distilled water ...
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bioRxiv - Cell Biology 2022Quote: ... at standard 5% CO2 and atmospheric O2 at 37°C in DMEM (5.5 mM glucose) supplemented with 10% FBS (Thermofisher #10437010), 50 μg/ml uridine (Sigma-Aldrich #U6381) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was then diluted 1:5 in 1x Assay buffer and processed according to the kit instructions (DNA Damage Competitive ELISA Kit, Invitrogen). The initial DNA concentration used in the assay was optimized to ensure the resulting values fall within the optimum range of kit detection limits (between Std3 and Std6) ...
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bioRxiv - Plant Biology 2022Quote: ... and the amplified DNA fragment and an annealed linker oligo (for GFP: primers 5 and 6) were introduced into the pENTR/D-TOPO vector (Invitrogen; sequences were confirmed by Sanger sequencing using primers 7 and 8 ...
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bioRxiv - Biochemistry 2022Quote: ... Chromatographic separation was carried out on an Aquasil C18 analytical column (4.6 mm x 150 mm, 5 mm; Thermo Scientific). MNA was eluted with the mobile phase consisting of acetonitrile (A ...
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bioRxiv - Molecular Biology 2022Quote: ... Blots were washed with TBST three times for 5 minutes and developed with the ECL system (ThermoFisher, Cat No. #34095) according to the manufacturer’s protocols.
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bioRxiv - Cancer Biology 2022Quote: ... All cells were maintained in a humidified 5% CO2 incubator at 37 °C and subcultured by 0.05% trypsin–0.5 mM EDTA solution (Gibco Life Technologies). Once the confluences reached suitable percentages ...
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bioRxiv - Cell Biology 2022Quote: ... was cultured in a gelatin/fibronectin matrix (5 μg fibronectin / 0.02% gelatin (m/v)-Sigma) and Claycomb culture medium supplemented with Glutamax™ (Gibco), 10% (v/v ...
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bioRxiv - Genomics 2022Quote: ... followed by seeding 5×104/cm2 of mouse embryonic fibroblast (MEF) cells (CellBiolabs, CBA-310) in MEF medium (DMEM [Gibco, 11965118] ...
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bioRxiv - Cancer Biology 2022Quote: ... Trp53loxP/loxP mice were incubated at 37°C and 5% CO2 in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher Scientific) containing 10% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... and HeLa-LAP-Spindly cells were grown in a humidified incubator at 37°C and 5% CO2 in DMEM (Gibco) containing 10% FCS Sigma-Aldrich) ...