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Citations for Thermo Fisher :
9851 - 9900 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... centrifuged at 300g for 5 min and resuspended in 3 mL of 1X ebioscience Red Blood Cell Lysis Buffer (ThermoFisher) for 5 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... was added to 7.5 μL of a master mix containing 5 μL of Power SYBR green mix (4367659, Applied Biosystems) and 2.5 μL of nuclease-free water with the nCoV_IP2 primers (nCoV_IP2-12669Fw ...
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bioRxiv - Neuroscience 2020Quote: ... was added to 7.5 μL of a master mix containing 5 μL of Power SYBR green mix (4367659, Applied Biosystems) and 2.5 μL of nuclease-free water containing predesigned primers (#249900 ...
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bioRxiv - Cancer Biology 2020Quote: ... 7.5 µL master mix was generated for each well in the following ratios: 5 µL genotyping Taqman mix (ThermoFisher Scientific), 0.5 µL of each the Met and Tfrc copy number probes (Table S5) ...
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bioRxiv - Cancer Biology 2019Quote: ... Click-iT Alexa Fluor 488 EdU (5-ethynyl-2’-deoxyuridine) imaging kit and Lipofectamine RNAiMAX was purchased from Life Technologies. Foetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... for 2 h at 37°C and 5% CO2 in the presence of dextran-rhodamine (0.5 mg/mL) (Molecular Probes). mAb-untreated Pf sporozoites were used to measure the maximum traversal capacity ...
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bioRxiv - Bioengineering 2020Quote: ... falciparum parasite culture was maintained in 5% human red blood cells purified from whole blood (Research Blood Components) with complete RPMI medium (Gibco) supplemented with hypoxanthine (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2019Quote: ... or human organoid basal culture media consisting of conditioned media produced using stably transfected L cells (Wnt 50%; R-spondin 10%; Noggin 5%) and the following growth factors: B12 1X (Invitrogen), Nicotinamide 10mM (Sigma-Aldrich) ...
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bioRxiv - Physiology 2019Quote: ... 100□μl of PBS solution containing 5□mg/ml of 70-kDa Texas Red-conjugated dextran (Thermo Fisher Scientific, USA) was injected intravenously prior to imaging ...
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bioRxiv - Cell Biology 2019Quote: Soft agar assays were carried out in 6-well plates previously coated with a 5 ml layer of medium containing 40% Dulbecco’s modified Eagle’s medium 2X (Thermo Fisher Scientific), 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... was added to the cells and incubated in the dark for 5-10 minutes prior to reading on a Varioskan LUX plate reader (ThermoFisher). Measurements were done in duplicate and relative luciferase units (RLU ...
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bioRxiv - Developmental Biology 2019Quote: ... then washed 2x in PBX for 5 minutes each and placed in a 5µg/ml solution of Hoechst 33342 (Life Technologies) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The loading solution contained a 1:10 mixture of 5 mM Fluo-4 AM in dry DMSO and PowerloadTM concentrate (Invitrogen), which was diluted 100-fold into extracellular Tyrode’s solution (NaCl 140mM ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were trapped at 20 μl/min in loading solvent (2% acetonitrile, 0.05% TFA) on a 5 mm x 300 μm C18 pepmap cartridge pre-column (Thermo Fisher Scientific / Dionex Softron GmbH ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR was conducted using a 5’ fluorescently-labelled primer (6-FAM, PET, NED, or VIC) (Applied Biosystems, Foster City, CA) and an unlabeled reverse primer ...
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bioRxiv - Bioengineering 2020Quote: ... centrifuged at 300 g for 5 min and seeded at a density of 50,000 cells/cm2 in Dulbecco’s Modified Eagle Medium (Invitrogen, Carlsbad, CA),supplemented with 10% v.v ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were then washed with PBS-T (3 × 5 min) and incubated with fluorophore-conjugated secondary antibodies (1:500 in blocking buffer, Invitrogen). Hoechst 33258 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells were cultured at 37°C and 5% CO2 in DMEM containing 10% [vol/vol] foetal bovine serum (Invitrogen). For passaging ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by 1 h incubation with 1:1000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... mouse BM-MSCs were first transfected with lentiCas9-Blast vector (Genomeditech) and selected with 5 μg/ml blasticidin S HCl (A1113903, Invitrogen). Then ...
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bioRxiv - Pathology 2020Quote: ... 5 µL of a 50/50 slurry of streptavidin coated magnetic beads (Dynabeads MyOne C1, Thermo Fisher Scientific, Waltham MA) in 1x-PBS was added to each cRASL reaction and incubated with gentle shaking for 15 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Longitudinal imaging of vascular leakage in C57BL/6 mouse was performed for 5 days after injecting 600 μg of 3kDa TRITC-dextran (LIF-D-3308, Invitrogen) and 600 μg of 2MDa FITC-dextran (LIF-D-7137 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were maintained at 37 °C in a humidified 5% CO2 incubator in Dulbecco’s Modified Eagle’s Medium (GIBCO, CA, USA) containing 10% fetal bovine serum (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: E14 ES cells were grown at 37°C in 5% CO2 on gelatin-coated plates in media comprising high glucose DMEM (Gibco), 15% foetal bovine serum (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: BMDM were isolated from tibia of WT and Thbs4-/- mice as described by others (36) and in our previous publication (5) and cultured in M-CSF (14-8983-62, ThermoFisher)/DMEM/F12 for 5 days ...
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bioRxiv - Neuroscience 2021Quote: ... tau proteins were measured with Tau-5 antibodies using ELISA according to manufacturer’s instructions (The Human Tau Solid-phase sandwich ELISA, #KHB0041, Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... Cells were washed once with CYE and further incubated 5 min with Alexa488- or Alexa561-labeled anti-rabbit secondary antibodies (Thermofisher). Cells were washed four times in CYE and concentrated 5-fold ...
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bioRxiv - Cancer Biology 2019Quote: All cells are grown at 37°C with 5% of CO2 with 100 UI/mL Penicillin and 100 µg/mL Streptomycin (Gibco). A673/TR/shEF (Carrillo et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... as well as human hepatoma Huh7.5 cells28 were maintained at 37 °C with 5% CO2 in Dulbecco’s Modified Medium (DMEM) (Invitrogen, Paisley, UK) containing 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... of SARS-CoV-2 S protein and a C-terminal polyhistidine tag was amplified and inserted into a pFasebac1 vector for expression in High-5 insect cells using the Bac-to-Bac expression system (Invitrogen). The resulting recombinant protein ...
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bioRxiv - Immunology 2019Quote: ... 100µl cell suspension was embedded by centrifugation (5’ at 1230 g at 30°C) in 100µl 2% Low Melting Agarose (LMA, Gibco BRL) in 0.1M cacodylate buffer ...
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bioRxiv - Microbiology 2021Quote: ... The cells were cultured at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Thermo Scientific; 31600-034) that contained 3.5 g/L D-glucose ...
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bioRxiv - Microbiology 2021Quote: ... The cells were left to fix for 1 h at room temperature before transferring 5 μl into 15 μl ProLongTM Gold Antifade mountant (ThermoFisher). For cell membrane staining ...
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bioRxiv - Molecular Biology 2021Quote: ... all proteolytic digests were acidified by addition of 10% formic acid to pH 2 and directly analyzed by LC-ESI-MS(/MS) using an a capillary BioBasic C18 reversed-phase column (BioBasic-18, 150 x 0.32 mm, 5 μm, Thermo Scientific), installed in an Ultimate U3000 HPLC system (Dionex) ...
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bioRxiv - Biochemistry 2021Quote: ... cells were cultured at 37 °C in humidified 95 % air with 5 % CO2 in Dulbecco’s modified essential medium (DMEM) (Gibco, Life Technologies) containing glucose (4.5 g/L) ...
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bioRxiv - Physiology 2021Quote: ASM cells and arteries were respectively loaded with 5 μM and 10 μM Fluo-4-AM (Invitrogen, Thermo Fisher Scientific) in a buffer solution containing 140 mM NaCl ...
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bioRxiv - Physiology 2021Quote: ASM cells and arteries were respectively loaded with 5 μM and 10 μM Fluo-4-AM (Invitrogen, Thermo Fisher Scientific) in a buffer solution containing 140 mM NaCl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HEK293T/17 cells were cultured at 37°C and 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, 10567-014) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO] ...
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bioRxiv - Developmental Biology 2021Quote: ... all cells were cultured at 37°C, 5% CO2 in growth medium (DMEM, 10% Embryonic Stem Cell qualified FBS (Gibco), NEAA ...
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bioRxiv - Cancer Biology 2021Quote: ... Suspension was centrifuged at 200g for 5 minute and cells were resuspended in 1ml of full medium (DMEM + 10% FBS; Gibco). Cells were either plated in 2D on cell culture treated flat bottom 96-well plates (Falcon ...
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bioRxiv - Molecular Biology 2021Quote: To label HELB with QDs we incubated a 1:5 molar ratio solution of biotinylated HELB and streptavidin-coated QDs 525 (Q10143, Invitrogen) for 30 min on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... resuspended in 5 mL PBS and crosslinked at room temperature with 1.5 mM ethylene glycol bis-succinimidyl succinate (EGS, Thermo Scientific). After 30 min of incubation ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized using the env V1/V3 Primer ID primer (HXB2 positions 6585-7208): 5’-GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNCAGTCCATTTTGCT CTACTAATGTTACAATGTGC-3’ and SuperScript III Reverse Transcriptase (Invitrogen). The final cDNA reaction contained the following ...
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bioRxiv - Microbiology 2020Quote: ... one or two guanines were added to the 5’ end of the guide sequence within the primer to ensure the format “5’-GG(N18-20)-3’” in order to facilitate in vitro transcription with MEGAscript T7 in vitro transcription kit (Ambion). Transcribed sgRNAs were purified with MEGAclear kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed four times with TBS-T for 5 minutes prior to secondary staining with HRP-conjugated goat anti-mouse and goat anti-rabbit antibodies (Invitrogen). For detection ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were initially trapped in C18 PepMap100 pre-column (300 µm inner diameter x 5 mm, 100A, Thermo Fisher Scientific) in Solvent A (Formic acid 0.1% (v/v) ...
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bioRxiv - Microbiology 2021Quote: Harvested cells were concentrated by centrifugation at 10,000 rpm for 1 min and immediately loaded on a 3% w/v agarose (in water) pad on a microscope slide supplemented with 5 µg/ml membrane dye Nile Red (Invitrogen). The agarose pad was made following the protocol described previously (15) ...
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bioRxiv - Microbiology 2021Quote: ... boiled at 100°C for 5 min and then separated on NuPAGE Novex 4-12% Bis-Tris midi gels (Invitrogen) using NuPAGE MES SDS Running buffer (Invitrogen) ...