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Citations for Takara Bio :
401 - 450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: B263R was amplified from gDNA of ASFV and cloned separately into vectors pCMV-Flag-N (635688; Clontech, Mountain View, CA, USA), pCMV-Myc-N (635689 ...
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bioRxiv - Molecular Biology 2024Quote: ... and transformed into StellarTM competent cells (Takara Bio) or Mix & Go Stbl3 competent cells (Zymo Research) ...
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bioRxiv - Molecular Biology 2024Quote: ... or Tks Gflex DNA polymerase (Takara Bio) by gradient touch-down PCR with cycler settings optimised for long-range synthesis ...
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bioRxiv - Molecular Biology 2024Quote: ... or DNA ligation kit (Takara) and transformed into StellarTM competent cells (Takara Bio ...
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bioRxiv - Physiology 2024Quote: ... and mCherry (1:500, Takara). Secondary antibodies from Invitrogen (Alexa Fluor 647 goat anti-rabbit ...
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bioRxiv - Physiology 2024Quote: ... (Cx43: 1:3000 custom-produced rabbit polyclonal; eGFP: 1:1000 mouse monoclonal (Clontech Laboratories Inc ...
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bioRxiv - Molecular Biology 2024Quote: ... HepG2-NTCP and Huh-7 cells with RNAi plus (TaKaRa, Japan) according to the manufacture’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... which incorporate a Gateway cassette between the SmaI and XhoI sites of the original pLacZi vectors (Clontech). Concurrently ...
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bioRxiv - Cell Biology 2024Quote: ... U-2OS cells stably infected with FLAG-CCND1/2xStrep-CCND2/HA-CCND3 were maintained in McCoy’s 5A medium supplemented with 10% Tet System Approved FBS (Takara, Clontech Laboratories) and 1% penicillin/streptomycin/L-glutamine (Corning Life Sciences) ...
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bioRxiv - Plant Biology 2024Quote: ... site-directed mutagenesis of gPLT2 was performed using the in-fusion HD cloning Kit (Takara Bio). The further reactions followed the in-fusion HD cloning Kit manual (Takara Bio) ...
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bioRxiv - Plant Biology 2024Quote: ... was amplified using CloneAmp™ HiFi PCR Premix (Takara Bio, USA) from genomic DNA and then sub-cloned into the pENTR/D/TOPO vector (Invitrogen) ...
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bioRxiv - Plant Biology 2024Quote: ... The PLT2-GFP fusion protein abundance was detected by using anti-GFP (cat#632381, 1:3,000 dilution; Clontech) and anti-ACTIN (cat#MA1-744 ...
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bioRxiv - Plant Biology 2024Quote: ... The further reactions followed the in-fusion HD cloning Kit manual (Takara Bio). Two sets of forward and reverse primers for gPLT2C112S ...
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bioRxiv - Plant Biology 2024Quote: ... and assembled using the In-Fusion HD Cloning Kit (Takara Bio). After Sanger sequencing validation ...
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bioRxiv - Plant Biology 2024Quote: ... The clones growing in the –L/-T liquid selection medium (Clontech) were diluted by a 10x series dilution and spotted onto –L/-T/-H medium (Clontech ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription reactions were performed using the PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA, Cat. No. 9750), solution was then removed ...
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bioRxiv - Physiology 2024Quote: Flies treated with the same procedure as the lifespan assay were collected after 7-day EF/Sham exposure and homogenized in RNAiso reagent (Takara Bio, Shiga, Japan). Ten to twenty flies were homogenized in one tube ...
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bioRxiv - Neuroscience 2024Quote: ... Developing glutamatergic neurons were transfected with peGFP-N1 (BD Biosciences Clontech) using a calcium phosphate transfection approach ...
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bioRxiv - Physiology 2024Quote: The plasmids were linearized with NotI (Takara Bio), and capped RNAs (cRNAs ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2 U/μL RNase Inhibitor (Takara, Tokyo, Japan)] ...
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bioRxiv - Plant Biology 2024Quote: The HIS3 and ADE2 reporter genes were employed to assess the protein interactions in the yeast strain Y2HGold (Takara Bio). Yeast transformation was carried out using the lithium acetate (LiAc)-mediated method ...
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bioRxiv - Neuroscience 2024Quote: Human embryonic kidney cells (Lenti-X 293T Cell line from Takara. Cat no. 632180) were grown in DMEM/F12 media (GIBCO ...
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bioRxiv - Plant Biology 2024Quote: ... equilibrated with 2 μg of anti-GFP antibody (Takara Bio, 632381). Beads were washed for 2 x 10 mins in low salt wash buffer ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were first incubated with ARIAD ligand at a concentration of 1 µM (AL; D/D Solubilizer; Takara) for the indicated times ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using PrimeScriptTM RT reagent kit (Takara, catalog no. RR037A), and qPCR analysis was done with power SYBR Green PCR master mix (Thermo Fisher ...
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bioRxiv - Pathology 2024Quote: ... we used the PrimeScript RT Reagent Kit (Perfect Real Time; Takara Bio Inc., Shiga, Japan). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviral titers were measured using Lenti-X GoStix (PT5185-2; Clontech, Takara Bio) following instructions provided by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... viral particles were collected and concentrated using a Lenti-X concentrator (631231; Takara Bio). Lentiviral titers were measured using Lenti-X GoStix (PT5185-2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviral titers were measured using Lenti-X GoStix (PT5185-2; Clontech, Takara Bio) following instructions provided by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... and ssDNA (M13mp18 single-stranded virion DNA, TaKaRa Bio, Kusatsu, Japan) were added in proportions of (in % ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to cDNA synthesis using PrimeScriptTM RT Master Mix (TaKaRa). qPCR was performed using QuantStudio6 Flex System (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... 3’ RACE was performed using 3’-Full RACE Core Set (TaKaRa, Kusatsu, Japan). PCR amplification on the 3’ UTR regions of AtCFI25a ...
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bioRxiv - Molecular Biology 2024Quote: ... and used with the mixture of specially designed primers (SWITCH2 and RT_universal_primer, Supplementary Table S2) for cDNA synthesis (SMARTScribe Reverse Transcriptase, TaKaRa, Kusatsu, Japan).
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bioRxiv - Neuroscience 2024Quote: ... and additionally with DNAoff (Takara) for library preparations.
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bioRxiv - Neuroscience 2024Quote: ... After the addition of 5 µl lysis buffer (0.2% Triton X-100, with 2 U/μl recombinant RNase inhibitor, Clontech) to the cap ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 (Takara, Shiga, Japan) and 0.32 µM of each primer according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The Genomic and RNA Profiling Core prepared libraries with the Takara SMARTer® Stranded Total RNA-Seq v3 - Pico Input Mammalian kit (Takara, p/n 634485). Using the KAPA Library Quantification kit for Illumina platforms (KAPA Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA-seq libraries were prepared using the SMART-Seq® Stranded Kit (634442, Takara), according to standard manufacturer’s protocol (Low-input Workflow ...
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bioRxiv - Immunology 2024Quote: ... and quantified using bulk RNA-seq (Psomagen) after construction of Illumina sequencing libraries using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara). Noise from low-expression transcripts was filtered ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.025 U μl−1 of SeqAmp polymerase (Takara Bio) and 0.5 μM Smartseq3 forward (5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATTGCGCAATG-3′ ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.6 μl PCR mix was dispensed to each well containing the following: 1× SeqAmp PCR buffer (Takara Bio), 0.025 U μl−1 of SeqAmp polymerase (Takara Bio ...
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bioRxiv - Molecular Biology 2024Quote: ... and the indicated type and amount of RNase Inhibitor (no RNAse inhibitor, 0.003 μl RNase Inhibitor (40 U/μl, Cat. 2313B, TaKaRa), SEQURNA Thermostable RNase inhibitor (Cat ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and pelleted using the Lenti-X Concentrator (631232; Clontech) according to the manufacturer’s instructions ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... DNA was purified using the standard phenol-chloroform extract method and subjected to DNase I (Takara, 2270 B) treatment and reverse transcription for DRIPc-seq library construction ...
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bioRxiv - Molecular Biology 2024Quote: ... We then introduced into this 303_p3E_GI_PA our previous RNAi cassette from plasmid pME-RNAi6415 using an “In-fusion (Takara)” cloning strategy ...
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bioRxiv - Molecular Biology 2024Quote: ... We conducted an In-fusion cloning reaction (In Fusion HD kit, Takara) following the manufacturer instructions to introduce the RNAi cassette into the digested intronic sequence ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1.2 U recombinant inhibitor (Cat. 2313B, TaKaRa) or SEQURNA Thermostable RNase inhibitor (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 U recombinant inhibitor (TaKaRa) for samples containing biological inhibitor ...
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bioRxiv - Molecular Biology 2024Quote: ... and 4 U recombinant inhibitor (Cat. 2313B, TaKaRa) or SEQURNA thermostable RNase inhibitor (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Fresh tubes of RRI (Cat. 2313B TaKaRa) were used in all comparisons in this study.