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Citations for Takara Bio :
401 - 450 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: HEK-293T (Takara cat. no. 632617), HEK293-EGFP (GenTarget ...
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bioRxiv - Neuroscience 2024Quote: ... and rabbit anti-dsRed (Takara Bio 632393; 1:200). Then ...
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bioRxiv - Molecular Biology 2024Quote: The quantitative RT-PCR (qRT-PCR) was performed with SYBRGreen II (Takara, Dalian, China). The 7500 system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... captured on column with 1 mL cobalt resin (Takara #635504), washed by 15 column volumes of PBS with 100 mM imidazole and 100 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was ligated with InFusion ligation enzyme (TaKaRa) on EPB71 backbone (Addgene plasmid #90018 ...
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bioRxiv - Molecular Biology 2024Quote: ... Ligated product was transformed to Stellar competent cells (TaKaRa) and plated on Agar plates with Carbenicillin (100µg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... using the SMARTer smRNA kit for Illumina (Takara), with few modifications ...
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bioRxiv - Neuroscience 2024Quote: ... The Clock-DN-P2A sequence was inserted into an AAV-EF1α-DIO-EYFP or AAV-EF1α-DO-EYFP vector backbone using In-Fusion cloning (Takara Bio).
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bioRxiv - Neuroscience 2024Quote: ... 0.4 U/ul RNAse inhibitor (Takara, 2313B), 0.2 U/ul SUPERase•In (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... RNAse inhibitor (0.4 U/ul; Takara, 2313B), SUPERase•In (0.2 U/ul ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-dsRed (Takara #632496. 1:1000), chicken anti-GFP (Abcam #13970 ...
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bioRxiv - Neuroscience 2024Quote: EGFP derived from pEGFP-C1 (Clontech) was cloned in-frame to the N-terminal domain of the mouse NFL gene (derived from pmNFL ...
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bioRxiv - Neuroscience 2024Quote: ... 0.02% Tween 20) and resuspend with Capture mix (50µl per ROI_index: 2xBWT, 0.5U/µl RRI (Takara)) ...
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bioRxiv - Neuroscience 2024Quote: ... and resuspended in PBS with 0.3%BSA and 1% recombinant RNase inhibitor (RRI, Takara Bio). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was incubated with TALON metal affinity resin (Takara) (3ml slurry per L culture ...
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bioRxiv - Microbiology 2024Quote: B263R was amplified from gDNA of ASFV and cloned separately into vectors pCMV-Flag-N (635688; Clontech, Mountain View, CA, USA), pCMV-Myc-N (635689 ...
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bioRxiv - Neuroscience 2024Quote: The primary human fibroblasts (HFs) and the corresponding iPSCs were purchased from Takara Bio (Kusatsu ...
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bioRxiv - Neuroscience 2024Quote: ... or In-Fusion cloning (Takara). The sequence of the constructs was confirmed by Sanger sequencing.
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bioRxiv - Microbiology 2024Quote: ... and then the viral DNA and the cDNA were processed with Klenow polymerase (Takara, Japan) in the presence of FR26RV-N ...
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bioRxiv - Microbiology 2024Quote: ... RNA and DNA were extracted using MiniBEST viral RNA/DNA extraction kit (Takara, Japan) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... A Premix Ex Taq™ (Probe qPCR) (TaKaRa, Japan) was used to perform qPCR following manufacturer’s instructions and each reaction consisted of 1 μl DNA template ...
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bioRxiv - Neuroscience 2024Quote: eGFP: EGFP (pAcGFP1-N1, Clontech)
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bioRxiv - Molecular Biology 2024Quote: ... All constructs were cloned using PCR amplification of complimentary fragments and In-Fusion HD assembly (Takara). pRRL-hPGK-mCherry-P2A-PuroR-T2A-FLAG-APEX2 was created by PCR amplifying hPGK-mCherry-P2A-PuroR-T2A from a synthesised gene fragment purchased from GeneWiz and APEX2 from pcDNA3-FLAG-APEX2-NES (a gift from Alice Ting67 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentivirus in the culture medium was concentrated using a lentivirus concentrator (Clontech Labs 3P #631231). One-third of the concentrated virus was used to infect 2.5 x 105 293T cells in the presence of 0.8 mg/ml polybrene ...
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bioRxiv - Molecular Biology 2024Quote: ... 4.9 μg DNA was transfected per dish containing a 1:1 molar ratio of pmCherry-N1 (Clontech) and of the pUC18-GFP or linear GFP substrate ...
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bioRxiv - Immunology 2024Quote: ... lentivirus was collected and concentrated using Lenti-X Concentrator (Clontech, #631231) overnight at 4°C followed by centrifugation according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... DNA was removed using DNaseI (Takara Bio Inc.). Reverse transcription of RNA was performed using PrimeScriptTM reagent Kit (Perfect Real Time ...
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bioRxiv - Neuroscience 2024Quote: A construct to overexpress Nr4a2 was generated with the In-Fusion HD Cloning Kit (Clontech) following supplier’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells were transfected using the CalPhos Mammalian Transfection kit (Clontech) following supplier’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... The In-Fusion reaction mixture were transformed into Stellar Competent Cells (Clontech) using the heat shock Method.
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-DsRed (1:500; 632496, Clontech), mouse anti-rat CD2 (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... and then the tip of the recording electrode was broken into the nested PCR reagent system (Prime ScriptTm RT reagent Kit with QDNA Eraser, Cat: RR0471, Takara), and a cDNA synthesis kit was used to synthesize cDNA following the manufacturer’s protocol (PrimeScriptm II1st Strand cDNA Synthesis Kit) ...
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bioRxiv - Neuroscience 2024Quote: Human embryonic kidney 293T cells (#632273, Takara Bio) were used to produce AAVs ...
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bioRxiv - Neuroscience 2024Quote: ... They were transfected with pAAV, pAAV2/1 (Penn Vector Core, Philadelphia, PA, USA) and pHelper plasmids (Takara Bio)52 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA (500 ng) was reverse transcribed to cDNA using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara, RR047A). qPCR was carried out using PowerUp™ SYBR™ Green Master Mix kit and detected by QuantStudio3 system (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: Total RNAs from scramble shRNA or TDP-43 shRNA treated cells were used to construct RNA-sequencing libraries using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit (Takara 634411), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and Doxycycline Hydrochloride (1ug/ml, 631311, Clontech). Plates are fixed in 4%PFA ...
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bioRxiv - Neuroscience 2024Quote: ... and Doxycycline Hydrochloride (1ug/ml, 631311, Clontech). Progenitor cells were cryopreserved for future use in micronic vials in CryoStorCS10 (07930 ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using TB Green Premix Ex Taq II (Takara Bio) and analysed in a Thermal Cycler Dice Real Time System Lite (Takara Bio) ...
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bioRxiv - Neuroscience 2024Quote: ... and analysed in a Thermal Cycler Dice Real Time System Lite (Takara Bio). The PCR primers used in the present study are listed in the Supplementary Table 4.
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bioRxiv - Neuroscience 2024Quote: ... The cDNA templates were synthesized from the purified RNA using the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio, Kusatsu, Japan) with oligo (dT)20 primers ...
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bioRxiv - Neuroscience 2024Quote: ... A fragment of the mutagenized GluA2 sequence between the BstEII/BspEI restriction sites was then subcloned into the AP-SEP-GluA2 construct (prepared as described in (30)) on a pBI-Tet on vector backbone (Clontech; #6152-1). For AP-SEP-GluA2 ET/YR-BirAER ...
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bioRxiv - Neuroscience 2024Quote: ... was amplified by PCR and inserted into the Cre-dependent AAV hSyn FLEx vector using BamHI/KpnI restriction sites.pTet-on (transactivator) was purchased from Clontech (Clontech; #P3070-5). Plasmids were prepared using the ZymoPURE Plasmid MaxiPrep Kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... and the protein was purified using cobalt affinity resin (1mL/L culture, Takara Bio USA, San Jose, CA). The protein was washed with 100 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 (Takara, Shiga, Japan) and 0.32 µM of each primer according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The Genomic and RNA Profiling Core prepared libraries with the Takara SMARTer® Stranded Total RNA-Seq v3 - Pico Input Mammalian kit (Takara, p/n 634485). Using the KAPA Library Quantification kit for Illumina platforms (KAPA Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA-seq libraries were prepared using the SMART-Seq® Stranded Kit (634442, Takara), according to standard manufacturer’s protocol (Low-input Workflow ...
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bioRxiv - Neuroscience 2024Quote: Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US). Plasmids expressing red fluorescent protein mRuby341 at 25–100 ng/µL under CAG promoter in combination with ASAP328 voltage sensor at 30–100 ng/µL under either CAG ...
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bioRxiv - Neuroscience 2024Quote: ... Cultures were tested regularly for mycoplasma (6601; TaKaRa PCR Mycoplasma Detection Set).
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bioRxiv - Molecular Biology 2024Quote: ... and ssDNA (M13mp18 single-stranded virion DNA, TaKaRa Bio, Kusatsu, Japan) were added in proportions of (in % ...