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Citations for Takara Bio :
451 - 500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... 1 mM dNTPs (Clontech), 1 U/mL RNase inhibitor ...
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bioRxiv - Biochemistry 2024Quote: ... a two-round PCR strategy was required to amplify the ORF using a high-fidelity PrimeStar ® DNA polymerase (Takara Europe, Saint-Germain-en-Laye, France). The first round of the nested PCR was carried out using the above cDNA as the template with primers designed in the 5’- and 3’-untranslated regions (UTR ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were processed for DNA library preparation (ThruPLEX DNA-seq Kit, Takara Bio), cleaned up with AMpure XP beads (Beckman Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was filtered using a 0.25 µm syringe filter and applied to Talon metal affinity resin (Takara) in 50mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit anti-dsRed (Takara 632496, 1:1,000–1:2,000), and Chicken anti-GFP (Aves GFP-1020 ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA synthesis was performed using a one-step PrimeScript RT-PCR kit (Takara Bio, Cat. RR057B, Japan) as per the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... using a random primer DNA labeling kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... in BrainPhys neuronal media (composed according to Bardy et al. 2015)74 and 2 µg/ml doxycycline (Takara 631311). Three days after plating and ...
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bioRxiv - Neuroscience 2024Quote: ... and iMatrix (T304, TaKaRa) coated µ- dishes (Cat ...
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bioRxiv - Immunology 2024Quote: ... one containing Mu Mx1 and one containing the Tol2 transposase were transfected into DF1 cells at 50-70% confluency using Xfect transfection reagent (Takara Bio, San Jose, CA) at a 5:1 ratio respectively ...
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bioRxiv - Immunology 2024Quote: ... The digested DNA was self-ligated using T4 DNA ligase (Takara, Japan). The ligated product was precipitated using 3M sodium acetate and 100% ethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... the plug was incubated in 160 μL of 1x M buffer containing 160 units of Nhe I (TaKaRa) overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... The CmR gene was amplified from plasmid pGro7 (Takara Bio) using the primer pair Cm-Vini-F/Cm-Vini-R ...
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bioRxiv - Biochemistry 2024Quote: ... After addition of DNaseI 1mM MgCl2 and 200μl 50% TALONR beads slurry (Clontech), the cell lysate was incubated at 10min at 4°C on a rolling shaker ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was reverse transcribed to cDNA with the PrimeScript RT kit (TaKaRa). cDNA was analysed by real-time PCR with the Light Cycler 480 SYBR Green I Master Mix (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... rat cerebral cortexes or fly brains using TRIzol and the reverse transcription was performed with PrimeScript™ RT Master Mix (Takara) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: HEK293T (Takara Cat. No. 632180), HeLa (ATCC CRM-CCL-2) ...
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bioRxiv - Molecular Biology 2024Quote: ... The qPCR reactions were carried out and the signals were detected using a real time PCR system (catalog no. TP950, TaKaRa). For RT-qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... Cloning was performed with commercially prepared Stellar competent cells (TaKaRa). Key plasmids were fully sequenced using plasmidsaurus and were deposited with Addgene ...
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bioRxiv - Developmental Biology 2024Quote: ... and cDNA synthesis was followed by retrotranscribing reverse transcription with primer transcript II reverse transcriptase (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... The RNA libraries were prepared with the SMART-Seq v4 PLUS Kit from TaKaRa following the TaKaRa protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Each sample was directly lysed in 4 μL of lysis buffer (including 0.2 μL of 1:1000 diluted external RNA controls consortium (ERCC) spike-in) and immediately reverse-transcribed using the PrimeScriptTM II Reverse Transcriptase (Takara, Cat# 2690A), and the cDNA library was constructed as the published Smart-seq2 method ...
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bioRxiv - Cell Biology 2024Quote: ... U-2OS cells stably infected with FLAG-CCND1/2xStrep-CCND2/HA-CCND3 were maintained in McCoy’s 5A medium supplemented with 10% Tet System Approved FBS (Takara, Clontech Laboratories) and 1% penicillin/streptomycin/L-glutamine (Corning Life Sciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... of the pDONR223 PCR product mixed with 243 fmol of each dsDNA insert sequences] and then mixed with 2µl of In-Fusion 5X HD Cloning Plus (Takara Bio #638909). The reaction was incubated for 15 minutes at 37°C and stored at −20°C ...
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bioRxiv - Developmental Biology 2024Quote: Single-cell cDNA synthesis was performed using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) according to the manufacturer’s protocol with some modifications as previously described17 ...
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bioRxiv - Molecular Biology 2024Quote: ... and into pGAD424 and pGBT9 vectors (Clontech) in frame with GAL4 Activation or DNA-binding domains ...
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bioRxiv - Cell Biology 2024Quote: ... including In-Fusion cloning (TaKaRa). Cloning was performed with commercially prepared Stellar competent cells (TaKaRa) ...
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bioRxiv - Synthetic Biology 2024Quote: ... restriction enzymes and T4 DNA ligase from NEB and PCRs were done using the PrimeSTAR Max DNA polymerase from Takara Bio ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... lentiviruses were produced in Lenti-X 293T cells (Takara Bio, Cat#632180) and lentivirus-containing supernatants were concentrated using Lenti-X concentrator (Takara Bio ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... and lentivirus-containing supernatants were concentrated using Lenti-X concentrator (Takara Bio, Cat#631231). NPCs were transduced and selected using 4ug/ml puromycin (Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... Total RNA was isolated from cells using TRIzol Reagent following the instructions and genomic DNA was removed using DNase I (TaKara). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNAs efficiency was assessed in vitro using the sgRNA In vitro Transcription and Screening kit (Clontech, 631439) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... using the PrimeScript RT Master Mix (Takara). We then amplified cDNA in Power SYBR (Thermo ...
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bioRxiv - Plant Biology 2024Quote: ... The CtBOT6 DNA fragment and the amplified vector were then merged using the In-Fusion HD Cloning Kit (TaKaRa). Ct4 transformed with the pBin-GFP-hph-CtBOT6 vector express CtBOT6 under the TrpC promoter control ...
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bioRxiv - Plant Biology 2024Quote: ... amplified promoter region fragments are inserted with KpnI and XhoI site in pAT006-Fluc-iG5_Rluc-iFAD2_F vector using In-Fusion HD Cloning Kit (Takara). Amplified BOT6 fragment was inserted with pGWB402 using pENTR™ Directional TOPO® Cloning Kits (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: Lenti-X 293T cells (Takara, Cat # 632180) were transfected with each CAR expression plasmid and Mirus Bio™ TransIT™-Lenti Transfection Reagent (Cat # MIR6600) ...
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bioRxiv - Cancer Biology 2024Quote: ... and then lentivirus was harvested and concentrated using Lenti-X Concentrator (Takara Bio, 631232).
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bioRxiv - Zoology 2024Quote: ... RNA was extracted using Isogen-LS (Nippon Gene) and RT-qPCR was performed using the One-Step PrimeScript RT-PCR Kit (Takara Bio) on a 7500 Real-Time RT-PCR System (Applied Biosystems) ...
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bioRxiv - Biophysics 2024Quote: ... The protein was purified to homogeneity using a combination of TALON Metal Affinity Resin (Takara) and gel filtration chromatography ...
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bioRxiv - Physiology 2024Quote: ... SYBR green (TaKaRa) and ABI7500 system (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... were cloned into pGBKT7-GW and pGADT7-GW (Takara Bio) vectors through Gateway cloning ...
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bioRxiv - Plant Biology 2024Quote: ... or Prime STAR HS DNA Polymerase (TaKaRa) with standard protocols.
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bioRxiv - Plant Biology 2024Quote: ... The first-strand cDNA synthesis was performed with the Prime Script RT Master Mix kit (Takara). Quantitative real-time PCR was performed at 60°C using Takyon No ROX SYBR 2X MasterMix blue dTTP (Eurogentec ...
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bioRxiv - Plant Biology 2024Quote: Single or multiple DNA fragments were cloned into fungal transformation vector using In-Fusion HD Cloning Kit (Clontech, USA). Briefly ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR amplification was first performed with PrimeSTAR GXL Premix (Takara Bio, R051A) to attach Nextera-style adapter sequences ...
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bioRxiv - Plant Biology 2024Quote: ... or the SMART-Seq mRNA LP Kit (Takara Bio USA) (for pre-parasitic J2 library generation) ...
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bioRxiv - Plant Biology 2024Quote: ... using the In-Fusion HD Cloning Kit (Clontech) kit ...
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bioRxiv - Plant Biology 2024Quote: ... the 5 μl samples were used as input into the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA) (for parasitic J3 library generation ...
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bioRxiv - Synthetic Biology 2024Quote: ... has been acquired from Takara Bio.