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Citations for Takara Bio :
451 - 500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ChIP-seq libraries were generated using DNA SMART ChIP-seq kits (Takara Bio, 634865) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were analysed by gel electrophoresis and positive products were purified using the NucleoSpin Gel and PCR clean up kit (Clontech, Takara Bio) before being sent for sequencing by Eurofins Genomics (Germany) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were analysed by gel electrophoresis and positive products were purified using the NucleoSpin Gel and PCR clean up kit (Clontech, Takara Bio) before being sent for sequencing by Eurofins Genomics (Germany) ...
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bioRxiv - Molecular Biology 2024Quote: ... The RNA extracted from the gonads underwent reverse transcription after concentration adjustment with the PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Japan).
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bioRxiv - Molecular Biology 2024Quote: ... and one μg of RNA was reverse transcribed using a cDNA Reverse Transcription Kit (RR037A, Takara). Final cDNA samples were used then used for quantitative real-time PCR assay by SYBR Green PCR Kit (RR820A ...
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bioRxiv - Molecular Biology 2024Quote: ... and plasmid DNA was isolated using NucleoSpin® Plasmid (NoLid) kit for miniprep (740499.250; Takara Bio) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Amplification was performed using the Advantage 2 Polymerase Mix (Clonetech, now Takara Bio USA, Mountain View, CA) and the following primers ...
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bioRxiv - Molecular Biology 2024Quote: ... Final cDNA samples were used then used for quantitative real-time PCR assay by SYBR Green PCR Kit (RR820A, Takara). Quantitative primers were designed based on the gene sequences by Sangon Biotech ...
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bioRxiv - Molecular Biology 2024Quote: ... The expression vector was produced by inserting the cDNA encoding the Rhino fragment into pGEX-5X-3 (Cytiva) by Infusion (Takara). The primers used are listed in Supplementary Table 1.
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bioRxiv - Molecular Biology 2024Quote: ... into LentiX-cells (Takara) according to standard procedures ...
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bioRxiv - Neuroscience 2024Quote: The annealed oligos were ligated with the pGuide-it-sgRNA1 Vector and prepared using the FastGene Plasmid Mini Kit (Nippon Genetics) and NucleoBond Xtra Midi (Takara Bio). Transfection into HEK-293T cells was performed according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... was produced and purified according to the Guide-it CRISPR/Cas9 Gesicle Production System protocol (Takara Bio) involving oligo-DNA pair annealing in a thermal cycler.
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bioRxiv - Neuroscience 2024Quote: ... AAV was purified 3–5 days after transfection using AAVpro Purification Kit Midi or Maxi (Takara Bio, Shiga, Japan). The viral concentration was measured by qRT-PCR.
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bioRxiv - Neuroscience 2024Quote: HEK-293T (Takara cat. no. 632617), HEK293-EGFP (GenTarget ...
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bioRxiv - Neuroscience 2024Quote: ... and rabbit anti-dsRed (Takara Bio 632393; 1:200). Then ...
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bioRxiv - Molecular Biology 2024Quote: The quantitative RT-PCR (qRT-PCR) was performed with SYBRGreen II (Takara, Dalian, China). The 7500 system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... captured on column with 1 mL cobalt resin (Takara #635504), washed by 15 column volumes of PBS with 100 mM imidazole and 100 mM NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was ligated with InFusion ligation enzyme (TaKaRa) on EPB71 backbone (Addgene plasmid #90018 ...
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bioRxiv - Molecular Biology 2024Quote: ... Ligated product was transformed to Stellar competent cells (TaKaRa) and plated on Agar plates with Carbenicillin (100µg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... using the SMARTer smRNA kit for Illumina (Takara), with few modifications ...
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bioRxiv - Neuroscience 2024Quote: ... The Clock-DN-P2A sequence was inserted into an AAV-EF1α-DIO-EYFP or AAV-EF1α-DO-EYFP vector backbone using In-Fusion cloning (Takara Bio).
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bioRxiv - Neuroscience 2024Quote: ... 0.4 U/ul RNAse inhibitor (Takara, 2313B), 0.2 U/ul SUPERase•In (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... RNAse inhibitor (0.4 U/ul; Takara, 2313B), SUPERase•In (0.2 U/ul ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-dsRed (Takara #632496. 1:1000), chicken anti-GFP (Abcam #13970 ...
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bioRxiv - Neuroscience 2024Quote: EGFP derived from pEGFP-C1 (Clontech) was cloned in-frame to the N-terminal domain of the mouse NFL gene (derived from pmNFL ...
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bioRxiv - Neuroscience 2024Quote: ... 0.02% Tween 20) and resuspend with Capture mix (50µl per ROI_index: 2xBWT, 0.5U/µl RRI (Takara)) ...
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bioRxiv - Neuroscience 2024Quote: ... and resuspended in PBS with 0.3%BSA and 1% recombinant RNase inhibitor (RRI, Takara Bio). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysate was incubated with TALON metal affinity resin (Takara) (3ml slurry per L culture ...
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bioRxiv - Microbiology 2024Quote: B263R was amplified from gDNA of ASFV and cloned separately into vectors pCMV-Flag-N (635688; Clontech, Mountain View, CA, USA), pCMV-Myc-N (635689 ...
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bioRxiv - Neuroscience 2024Quote: The primary human fibroblasts (HFs) and the corresponding iPSCs were purchased from Takara Bio (Kusatsu ...
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bioRxiv - Neuroscience 2024Quote: ... or In-Fusion cloning (Takara). The sequence of the constructs was confirmed by Sanger sequencing.
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bioRxiv - Microbiology 2024Quote: ... and then the viral DNA and the cDNA were processed with Klenow polymerase (Takara, Japan) in the presence of FR26RV-N ...
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bioRxiv - Microbiology 2024Quote: ... RNA and DNA were extracted using MiniBEST viral RNA/DNA extraction kit (Takara, Japan) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... A Premix Ex Taq™ (Probe qPCR) (TaKaRa, Japan) was used to perform qPCR following manufacturer’s instructions and each reaction consisted of 1 μl DNA template ...
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bioRxiv - Neuroscience 2024Quote: eGFP: EGFP (pAcGFP1-N1, Clontech)
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bioRxiv - Molecular Biology 2024Quote: ... All constructs were cloned using PCR amplification of complimentary fragments and In-Fusion HD assembly (Takara). pRRL-hPGK-mCherry-P2A-PuroR-T2A-FLAG-APEX2 was created by PCR amplifying hPGK-mCherry-P2A-PuroR-T2A from a synthesised gene fragment purchased from GeneWiz and APEX2 from pcDNA3-FLAG-APEX2-NES (a gift from Alice Ting67 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentivirus in the culture medium was concentrated using a lentivirus concentrator (Clontech Labs 3P #631231). One-third of the concentrated virus was used to infect 2.5 x 105 293T cells in the presence of 0.8 mg/ml polybrene ...
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bioRxiv - Molecular Biology 2024Quote: ... 4.9 μg DNA was transfected per dish containing a 1:1 molar ratio of pmCherry-N1 (Clontech) and of the pUC18-GFP or linear GFP substrate ...
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bioRxiv - Immunology 2024Quote: ... lentivirus was collected and concentrated using Lenti-X Concentrator (Clontech, #631231) overnight at 4°C followed by centrifugation according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... DNA was removed using DNaseI (Takara Bio Inc.). Reverse transcription of RNA was performed using PrimeScriptTM reagent Kit (Perfect Real Time ...
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bioRxiv - Neuroscience 2024Quote: A construct to overexpress Nr4a2 was generated with the In-Fusion HD Cloning Kit (Clontech) following supplier’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293T cells were transfected using the CalPhos Mammalian Transfection kit (Clontech) following supplier’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... The In-Fusion reaction mixture were transformed into Stellar Competent Cells (Clontech) using the heat shock Method.
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-DsRed (1:500; 632496, Clontech), mouse anti-rat CD2 (1:200 ...
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bioRxiv - Neuroscience 2024Quote: The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... and then the tip of the recording electrode was broken into the nested PCR reagent system (Prime ScriptTm RT reagent Kit with QDNA Eraser, Cat: RR0471, Takara), and a cDNA synthesis kit was used to synthesize cDNA following the manufacturer’s protocol (PrimeScriptm II1st Strand cDNA Synthesis Kit) ...
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bioRxiv - Neuroscience 2024Quote: Human embryonic kidney 293T cells (#632273, Takara Bio) were used to produce AAVs ...
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bioRxiv - Neuroscience 2024Quote: ... They were transfected with pAAV, pAAV2/1 (Penn Vector Core, Philadelphia, PA, USA) and pHelper plasmids (Takara Bio)52 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA (500 ng) was reverse transcribed to cDNA using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara, RR047A). qPCR was carried out using PowerUp™ SYBR™ Green Master Mix kit and detected by QuantStudio3 system (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: Total RNAs from scramble shRNA or TDP-43 shRNA treated cells were used to construct RNA-sequencing libraries using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit (Takara 634411), according to the manufacturer’s instructions ...