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Citations for Takara Bio :
201 - 250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: CD8+ T-cells were exposed to the SMARTerTM RACE cDNA Amplification Kit (Clontech/Takara) to identify and annotate ROPN1/B epitope-specific TCRα- and β-chains based on Kunert et al ...
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bioRxiv - Immunology 2024Quote: ... qRT-PCR was conducted using TB Green fluorescence (TaKaRa) on a MasterCycler EP Realplex 4 thermal cycler (Eppendorf ...
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bioRxiv - Immunology 2024Quote: CD8+ T-cells were exposed to the SMARTerTM RACE cDNA Amplification Kit (Clontech/Takara) to identify and annotate ROPN1/B epitope-specific TCRα- and β-chains based on Kunert et al ...
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bioRxiv - Immunology 2024Quote: ... and cloned into the PiggyBac vector (a kind gift from Dr. P.J. French, Erasmus MC, Rotterdam, the Netherlands) using In Fusion cloning kit (Takara). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... Fifteen microliter reactions were analyzed as outlined by the manufacturer (TaKaRa). The relative fold-changes of the transcripts were calculated using the comparative CT(2-ΔΔCT ...
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bioRxiv - Immunology 2024Quote: ... A total of 1 μg of total RNA was reverse-transcribed with random primers using the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... The band was extracted and purified with Nucleospin Gel Extraction Kit (Takara). The gel-purified DNA was then further purified with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was run on a 1% agarose gel at 85 V for 35 min and gel extracted using the Nucleospin Gel Extraction Kit (Takara). We then used PCR with KOD Polymerase 2x Mastermix (Millipore Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were homogenized with RNAiso (Takara, Otsu, Japan) and incubated in room temperature for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... using TB Green Premix Ex Taq (TaKaRa) and 0.2 μM of each primer ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription of the RNA using a primer designed to the constant region of the barcoded adaptor with addition of an adapter to the 3’ end of the cDNA by template switching using SMARTScribe (Clontech) as described;86 (ii ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 cells were collected in the center of a 1.5 ml centrifuge tube containing 4.75 μl SMART-seq reaction buffer (Takara) using a BD FACSymphony S6 (BD Bioscience) ...
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bioRxiv - Cancer Biology 2024Quote: RNA was prepared and cDNA was synthesized with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) and ran the Agilent Tapestation to assess cDNA product ...
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bioRxiv - Molecular Biology 2024Quote: - IgG detector solution V2 (1:2000 dilution, Takara, Cat# T7122A-1).
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bioRxiv - Molecular Biology 2024Quote: ... Complementary DNA (cDNA) was amplificated with SYBR Premix Ex TaqTM (TaKaRa Bio, Otsu, Japan). RT-qPCR was performed using SYBR Green PCR master mix (Life Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... which was conducted using oligo-dT-primed reverse transcription with SMARTScribe reverse transcriptase (Clontech, Cat#: 639538) and a locked nucleic acid containing template-switching oligonucleotide (TSO ...
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bioRxiv - Developmental Biology 2024Quote: ... ThruPLEX DNA-seq Kit (Takara) was used to construct the CUT&RUN DNA library for sequencing on an Illumina platform ...
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bioRxiv - Genetics 2024Quote: ... 1 mM dNTPs (Clontech), 1 U/mL RNase inhibitor ...
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bioRxiv - Biochemistry 2024Quote: ... a two-round PCR strategy was required to amplify the ORF using a high-fidelity PrimeStar ® DNA polymerase (Takara Europe, Saint-Germain-en-Laye, France). The first round of the nested PCR was carried out using the above cDNA as the template with primers designed in the 5’- and 3’-untranslated regions (UTR ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were processed for DNA library preparation (ThruPLEX DNA-seq Kit, Takara Bio), cleaned up with AMpure XP beads (Beckman Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was filtered using a 0.25 µm syringe filter and applied to Talon metal affinity resin (Takara) in 50mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit anti-dsRed (Takara 632496, 1:1,000–1:2,000), and Chicken anti-GFP (Aves GFP-1020 ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA synthesis was performed using a one-step PrimeScript RT-PCR kit (Takara Bio, Cat. RR057B, Japan) as per the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... using a random primer DNA labeling kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... in BrainPhys neuronal media (composed according to Bardy et al. 2015)74 and 2 µg/ml doxycycline (Takara 631311). Three days after plating and ...
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bioRxiv - Neuroscience 2024Quote: ... and iMatrix (T304, TaKaRa) coated µ- dishes (Cat ...
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bioRxiv - Immunology 2024Quote: ... one containing Mu Mx1 and one containing the Tol2 transposase were transfected into DF1 cells at 50-70% confluency using Xfect transfection reagent (Takara Bio, San Jose, CA) at a 5:1 ratio respectively ...
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bioRxiv - Immunology 2024Quote: ... The digested DNA was self-ligated using T4 DNA ligase (Takara, Japan). The ligated product was precipitated using 3M sodium acetate and 100% ethanol ...
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bioRxiv - Molecular Biology 2024Quote: ... The plug was equilibrated twice in 1 mL of 1× M buffer (TaKaRa) by rotating the tube for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... the plug was incubated in 160 μL of 1x M buffer containing 160 units of Nhe I (TaKaRa) overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... The CmR gene was amplified from plasmid pGro7 (Takara Bio) using the primer pair Cm-Vini-F/Cm-Vini-R ...
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bioRxiv - Biochemistry 2024Quote: ... After addition of DNaseI 1mM MgCl2 and 200μl 50% TALONR beads slurry (Clontech), the cell lysate was incubated at 10min at 4°C on a rolling shaker ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was reverse transcribed to cDNA with the PrimeScript RT kit (TaKaRa). cDNA was analysed by real-time PCR with the Light Cycler 480 SYBR Green I Master Mix (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... rat cerebral cortexes or fly brains using TRIzol and the reverse transcription was performed with PrimeScript™ RT Master Mix (Takara) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: HEK293T (Takara Cat. No. 632180), HeLa (ATCC CRM-CCL-2) ...
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bioRxiv - Molecular Biology 2024Quote: ... The qPCR reactions were carried out and the signals were detected using a real time PCR system (catalog no. TP950, TaKaRa). For RT-qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... Cloning was performed with commercially prepared Stellar competent cells (TaKaRa). Key plasmids were fully sequenced using plasmidsaurus and were deposited with Addgene ...
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bioRxiv - Developmental Biology 2024Quote: ... and cDNA synthesis was followed by retrotranscribing reverse transcription with primer transcript II reverse transcriptase (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... The RNA libraries were prepared with the SMART-Seq v4 PLUS Kit from TaKaRa following the TaKaRa protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Each sample was directly lysed in 4 μL of lysis buffer (including 0.2 μL of 1:1000 diluted external RNA controls consortium (ERCC) spike-in) and immediately reverse-transcribed using the PrimeScriptTM II Reverse Transcriptase (Takara, Cat# 2690A), and the cDNA library was constructed as the published Smart-seq2 method ...
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bioRxiv - Cell Biology 2024Quote: ... U-2OS cells stably infected with FLAG-CCND1/2xStrep-CCND2/HA-CCND3 were maintained in McCoy’s 5A medium supplemented with 10% Tet System Approved FBS (Takara, Clontech Laboratories) and 1% penicillin/streptomycin/L-glutamine (Corning Life Sciences) ...
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bioRxiv - Cancer Biology 2024Quote: ... of the pDONR223 PCR product mixed with 243 fmol of each dsDNA insert sequences] and then mixed with 2µl of In-Fusion 5X HD Cloning Plus (Takara Bio #638909). The reaction was incubated for 15 minutes at 37°C and stored at −20°C ...
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bioRxiv - Developmental Biology 2024Quote: Single-cell cDNA synthesis was performed using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) according to the manufacturer’s protocol with some modifications as previously described17 ...
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bioRxiv - Molecular Biology 2024Quote: ... and into pGAD424 and pGBT9 vectors (Clontech) in frame with GAL4 Activation or DNA-binding domains ...
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bioRxiv - Cell Biology 2024Quote: ... including In-Fusion cloning (TaKaRa). Cloning was performed with commercially prepared Stellar competent cells (TaKaRa) ...
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bioRxiv - Synthetic Biology 2024Quote: ... restriction enzymes and T4 DNA ligase from NEB and PCRs were done using the PrimeSTAR Max DNA polymerase from Takara Bio ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... lentiviruses were produced in Lenti-X 293T cells (Takara Bio, Cat#632180) and lentivirus-containing supernatants were concentrated using Lenti-X concentrator (Takara Bio ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... and lentivirus-containing supernatants were concentrated using Lenti-X concentrator (Takara Bio, Cat#631231). NPCs were transduced and selected using 4ug/ml puromycin (Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... Total RNA was isolated from cells using TRIzol Reagent following the instructions and genomic DNA was removed using DNase I (TaKara). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNAs efficiency was assessed in vitro using the sgRNA In vitro Transcription and Screening kit (Clontech, 631439) following the manufacturer’s protocol ...