Labshake search
Citations for Takara Bio :
201 - 250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Monoclonal Antibody (JL-8) (Clontech). Anti-mouse IgG was used as a secondary antibody.
-
bioRxiv - Microbiology 2024Quote: ... and ligated between the NcoI and BamHI sites of the pTM1 vector with In-Fusion® Snap Assembly Master Mix (TAKARA) according to the manufacturer’s protocols ...
-
bioRxiv - Microbiology 2024Quote: ... The HA1 subunit was divided into three fragments for PCR (PrimeSTAR Max DNA Polymerase, Takara Bio), seven Ns and partial sequencing adapters were added in the primer as the barcode ...
-
bioRxiv - Immunology 2024Quote: ... genes encoding the variable regions of C7 and C74 heavy chains were cloned into a pMN vector with a human CH1 domain and a C-terminal His-tag using In-Fusion cloning system (Takara Bio #639649). The full light chains of C7 and C74 were cloned into the pMN vector without any purification tag using the same method ...
-
bioRxiv - Immunology 2024Quote: ... Cultures were harvested after 7 days and Fabs were purified from culture supernatant using His60 Ni Superflow resin (Takara Bio #635660). Fabs were eluted from the column using a buffer of 50 mM Tris ...
-
bioRxiv - Immunology 2024Quote: ... The two PCR products were cloned via In-Fusion HD Cloning (Takara) into the pCAGGS-ARM/GPC plasmid digested with XbaI and KpnI replacing a segment of the wild-type GPC sequence ...
-
bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using PrimeScript RT-PCR Kit from TaKaRa (#RR037A) (Otsu ...
-
bioRxiv - Immunology 2024Quote: ... The full-length CD177 cDNA inserts were sub-cloned into pBMN-I-EGFP retroviral vector digested with Bam HI and EcoR I restriction enzymes using InFusion HD Cloning Kit (Takara Bio USA, Mountain View, Ca) according to the product manual ...
-
bioRxiv - Immunology 2024Quote: ... RNA was reverse transcribed and cDNA amplified by in vitro transcription with the TaKaRa SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa, Cat. No. 634889) or a low input method using NEB Next® Ultra RNA Library Prep Kit for Illumina® (NEB ...
-
bioRxiv - Immunology 2024Quote: ... equipped with SYBR green detector (TaKaRa Bio, USA) [13] ...
-
bioRxiv - Immunology 2024Quote: ... Steps were performed on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step ...
-
bioRxiv - Immunology 2024Quote: ... The RNeasy kit and the cDNA Synthesis Kit (Takara, Cat#: 9767, RR047A) was used to extract total RNA and prepare cDNA following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... and in-fusion cloned (Takara) into the GPC locus of the mPol1 LCMV S NP/BsmBI cassette digested with BsmBI ...
-
bioRxiv - Cell Biology 2024Quote: ... genomic DNA was purified with Terra™ PCR Direct Genotyping Kit (Takara), followed by PCR amplification and Sanger sequencing of specific genomic regions encompassing the target site.
-
bioRxiv - Cell Biology 2024Quote: ... 0.2µM of each primer (Table S4) and SYBR® Premix ExTaqTM (Takara) according to the manufacture instructions ...
-
bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using RNAiso Plus reagent (Takara, 9019) and reverse transcribed into cDNA using the Hifair® AdvanceFast One-step RT-gDNA Digestion SuperMix (Yeasen ...
-
bioRxiv - Cell Biology 2024Quote: ... HaloTag-mouse ACVR1B-HiBiT and HaloTag-mouse ACVR2B-HiBiT lentiviruses were generated in HEK293T cells using Lenti-X Packaging Single Shots (Takara Bio, 631276) and concentrated 20-fold with Lenti Concentrator (OriGene ...
-
bioRxiv - Biophysics 2024Quote: ... The purification process involved an affinity chromatography step using cobalt affinity resin (TALON, Clontech), followed by a size exclusion chromatography (SEC ...
-
bioRxiv - Biochemistry 2024Quote: ... and the supernatant was batch bound to TALON cobalt-affinity resin (Takara) overnight at 4 °C ...
-
bioRxiv - Bioengineering 2024Quote: Escherichia coli strain HST08 (Takara Bio, Otsu, Japan) was used as the host for recombinant DNA manipulation ...
-
bioRxiv - Bioengineering 2024Quote: ... The mCherry fragment carried by pQLL plasmid with AmpR was synthesized by Beijing Liuhe BGI and then amplified using PrimeSTAR® Max DNA Polymerase (Takara Bio). The plasmid pmCherry was generated by fusing the lacI-Ptrc inducible system and mCherry into the vector pBBR1MCS-5 using In-Fusion® Snap Assembly Master Mix (Takara Bio) ...
-
bioRxiv - Bioengineering 2024Quote: ... coli DH5α (Takara Bio) was used for molecular cloning and cultivated in Luria-Bertani (LB ...
-
bioRxiv - Bioengineering 2024Quote: ... CD11b-specific amplicons were generated via 3-step nested PCR using PrimeSTAR GXL (Takara Bio, Kusatsu, Shiga, Japan) according to the manufacturer’s protocol with a 60°C annealing temperature ...
-
bioRxiv - Bioengineering 2024Quote: ... rapalog AP21967 (Takara) was added to a final concentration of 500 nM ...
-
bioRxiv - Bioengineering 2024Quote: ... Constructs were cloned with InFusion (Clontech) using backbone PCR ...
-
bioRxiv - Bioengineering 2024Quote: ... RNA concentrations were normalized and reverse transcription was performed using the RNA to cDNA EcoDry premix kit (Takara Bio USA; San Jose, CA, USA). Taqman universal master mix and Taqman probes (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2024Quote: We used a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa) for cDNA amplification according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... All the other plasmids used in this study are cloned by following the standard protocol of Infusion HD (Takara).
-
bioRxiv - Cancer Biology 2024Quote: ... Retroviral transduction was achieved by spinoculation of 3 × 106 mouse T cells on retronectin-coated plates (Takara Bio) with neat retroviral supernatant harvested from 293T packaging cells (2000xg ...
-
bioRxiv - Biochemistry 2024Quote: The SUGCT coding sequence was amplified by PCR by using PrimeStar GXL polymerase (Takara Bio Inc) using the following primers (start and stop codon in bold) ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Brain sections were transferred to positively charged glass histology slides (Fisherbrand) and immunostained with mCherry (rabbit-anti-DsRed; Takara Bio ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... The following primary antibodies were used: mCherry (rabbit-anti-DsRed; Takara Bio ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 250 nM Shield1 (Takara Bio) for 21 hours.
-
bioRxiv - Cancer Biology 2024Quote: ... pelleted overnight using Retro-X-Concentrator (Takara Bio, 631456) and resuspended in complete TexMACS the next morning ...
-
bioRxiv - Cancer Biology 2024Quote: ... Meanwhile, untreated 6-well cell culture plates (Falcon, 351146) were coated with 15 µg/ml retronectin solution (Takara Bio, T100A) overnight at 4 °C ...
-
bioRxiv - Plant Biology 2024Quote: ... which incorporate a Gateway cassette between the SmaI and XhoI sites of the original pLacZi vectors (Clontech). Concurrently ...
-
bioRxiv - Cell Biology 2024Quote: ... U-2OS cells stably infected with FLAG-CCND1/2xStrep-CCND2/HA-CCND3 were maintained in McCoy’s 5A medium supplemented with 10% Tet System Approved FBS (Takara, Clontech Laboratories) and 1% penicillin/streptomycin/L-glutamine (Corning Life Sciences) ...
-
bioRxiv - Plant Biology 2024Quote: ... site-directed mutagenesis of gPLT2 was performed using the in-fusion HD cloning Kit (Takara Bio). The further reactions followed the in-fusion HD cloning Kit manual (Takara Bio) ...
-
bioRxiv - Plant Biology 2024Quote: ... was amplified using CloneAmp™ HiFi PCR Premix (Takara Bio, USA) from genomic DNA and then sub-cloned into the pENTR/D/TOPO vector (Invitrogen) ...
-
bioRxiv - Plant Biology 2024Quote: ... The PLT2-GFP fusion protein abundance was detected by using anti-GFP (cat#632381, 1:3,000 dilution; Clontech) and anti-ACTIN (cat#MA1-744 ...
-
bioRxiv - Plant Biology 2024Quote: ... The further reactions followed the in-fusion HD cloning Kit manual (Takara Bio). Two sets of forward and reverse primers for gPLT2C112S ...
-
bioRxiv - Physiology 2024Quote: ... and mCherry (1:500, Takara). Secondary antibodies from Invitrogen (Alexa Fluor 647 goat anti-rabbit ...
-
bioRxiv - Physiology 2024Quote: ... (Cx43: 1:3000 custom-produced rabbit polyclonal; eGFP: 1:1000 mouse monoclonal (Clontech Laboratories Inc ...
-
bioRxiv - Physiology 2024Quote: The plasmids were linearized with NotI (Takara Bio), and capped RNAs (cRNAs ...
-
bioRxiv - Neuroscience 2024Quote: ... 0.2 U/μL RNase Inhibitor (Takara, Tokyo, Japan)] ...
-
bioRxiv - Plant Biology 2024Quote: The HIS3 and ADE2 reporter genes were employed to assess the protein interactions in the yeast strain Y2HGold (Takara Bio). Yeast transformation was carried out using the lithium acetate (LiAc)-mediated method ...
-
bioRxiv - Plant Biology 2024Quote: ... and assembled using the In-Fusion HD Cloning Kit (Takara Bio). After Sanger sequencing validation ...
-
bioRxiv - Plant Biology 2024Quote: ... The clones growing in the –L/-T liquid selection medium (Clontech) were diluted by a 10x series dilution and spotted onto –L/-T/-H medium (Clontech ...
-
bioRxiv - Plant Biology 2024Quote: ... Reverse transcription reactions were performed using the PrimeScript II 1st strand cDNA Synthesis Kit (TAKARA, Cat. No. 9750), solution was then removed ...
-
bioRxiv - Physiology 2024Quote: Flies treated with the same procedure as the lifespan assay were collected after 7-day EF/Sham exposure and homogenized in RNAiso reagent (Takara Bio, Shiga, Japan). Ten to twenty flies were homogenized in one tube ...