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Citations for Takara Bio :
201 - 250 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... DNaseI-treated total RNA (4 µg) was reverse transcribed using Primescript reverse transcriptase (Takara Bio) and random hexamers (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Lenti-XTM 293T (Takara, 632810) cells were transfected GFP-LC3 plasmid ...
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bioRxiv - Biochemistry 2024Quote: The Upf1-HD-His6 wild-type protein was first purified on a TALONTM resin (Clontech) equilibrated with buffer A200 ...
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bioRxiv - Microbiology 2024Quote: ... M4B and M4 midgut regions were harvested in RNAiso Plus (Takara Bio). This resulted in 24 samples (Apo and Sym insects × 4 timepoints × 3 midgut regions) ...
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bioRxiv - Biochemistry 2024Quote: ... The linear PCR product was then re-circularized with In-Fusion Cloning Master Mix (Takara Bio, 638948) according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... sgRNA inserts were PCR amplified using Ex Taq DNA Polymerase (Takara, cat#RR001A) from sufficient genome equivalents of DNA to achieve an average coverage of >200x of the sgRNA library ...
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bioRxiv - Cancer Biology 2024Quote: ... mRNA was reversed transcribed into cDNA using PrimeScript™ RT Master Mix (Takara Bio #RR036B). cDNA was then used for qPCR with SsoAdvanced Universal SYBR (Bio-Rad #1726275 ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTag-mouse ACVR1B-HiBiT and HaloTag-mouse ACVR2B-HiBiT lentiviruses were generated in HEK293T cells using Lenti-X Packaging Single Shots (Takara Bio, 631276) and concentrated 20-fold with Lenti Concentrator (OriGene ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was purified with Terra™ PCR Direct Genotyping Kit (Takara), followed by PCR amplification and Sanger sequencing of specific genomic regions encompassing the target site.
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then diluted 1:5 to improve binding efficiency and incubated overnight on the rotisserie at 4°C with TALON Cobalt Resin (Takara Bio). The next day the resin was washed with 10 column volumes of Membrane Buffer 1 ...
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bioRxiv - Biophysics 2024Quote: ... for 35 min at 4°C and the supernatant was incubated overnight on the rotisserie at 4°C with TALON Cobalt Resin (Takara Bio). The next day the resin was washed with 10 column volumes of Purification Buffer and 10 column volumes of Purification Buffer with 10 mM imidazole before elution using Purification Buffer with 300 mM imidazole ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR fragments containing pLib vector backbone (Clontech) and EF1A promoter ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then diluted 1:4 to improve binding efficiency and incubated overnight on the rotisserie at 4°C with TALON Cobalt Resin (Takara Bio). The next day the resin was washed with 10 column volumes of Membrane Buffer 2 and 10 column volumes of Membrane Buffer 2 with 20 mM imidazole before elution with Membrane Buffer 2 with 300 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... and retronectin (20ng/uL; Takara T100B) in PBS overnight at 4C ...
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bioRxiv - Cell Biology 2024Quote: ... The absence of ATG14 transcripts was verified by Reverse Transcription PCR (RT-PCR) on cDNA synthesized from total RNA extracted from the cell samples using Terra™ PCR Direct Polymerase Mix (TAKARA, 639270). Total RNA was isolated using the NucleoSpin RNA II kit (Macherey-Nagel) ...
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bioRxiv - Cell Biology 2024Quote: ... using In-Fusion® HD Cloning Kit (Takara Bio, #639650). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... two PCRs were performed using li-Strep_ss-SBP-EGFP-Ecadherin (a gift plasmid from F. Perez) (Boncompain et al., 2012) and pEGFP-C1 (Clontech) as templates and the following primer pairs (GAT GCA CCC GGG AGG CGC GCC ATG and CTC CTC GCC CTT GCT CAC ACC TGC AGG TGG TTC ACG ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was collected and incubated with TALON IMAC resin (Clontech) at 4 °C for 2 h in the presence of 20 mM imidazole ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS, Clontech) and 50 μg/ml gentamicin (Life Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... concentrated with Lenti-X™ Concentrator (Takara, #631231), and resuspended in 1/10th volume of fresh Type 2 medium [12] ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μg of genomic DNA was used as a template using Titanium Taq PCR kit (Takara). The sequence information for the primers used for barcode amplification was kindly provided by Novartis and can be found in Supplementary Table 4 ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 μg of genomic DNA per sample was used as template for PCR to amplify the sgRNA region using Ex Taq DNA polymerase (Takara). Next Generation Sequencing (NGS ...
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bioRxiv - Cancer Biology 2024Quote: ... The plasmid libraries were then transfected into Lenti-X cells (Takara) to produce lentiviral pools ...
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bioRxiv - Cancer Biology 2024Quote: ... and media was exchanged for DMEM with 10% Tet-free FBS (Clontech). Supernatants were collected and 0.45 μm filtered at 48 and 72 hours post-transfection and immediately used for infection of MeWo-rtTA target cells in the presence of 8 μg/ml of polybrene (Fisher) ...
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bioRxiv - Cancer Biology 2024Quote: pDual_dsCas9_Venus was prepared by In Fusion cloning (Takara, #638952) using EDCPV vector (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcode sequence of each clone was PCR amplified directly from the cells using Terra PCR Direct Polymerase kit (Takara) for Sanger sequencing using previously described primers (5).
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bioRxiv - Cancer Biology 2024Quote: ... we sub-cloned TVA-2A-mCherry-2A-oGlycoprotein into a lentiviral vector (‘pFU-‘) using In-Fusion cloning (Takara). TVA-2A-mCherry-2A-oGlycoprotein was amplified from p306 (Zurich virus core) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was used for library preparation with SMARTer® StrandedTotal RNA-Seq Kit v3 (Takara Bio) with rRNA removal and sequenced on Illumina Novaseq 6000(50 bp paired-end mode ...
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bioRxiv - Cell Biology 2024Quote: ... ovaries were collected in 1ml of TRIzol (Takara - 9108). Then ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration was determined by using Bradford assay kit (Takara). For the vehicle control and inhibitor-treated or 5 ...
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bioRxiv - Cell Biology 2024Quote: ... Actin and GAPDH (primers in Table 1) in ovaries by using TB Green syber mix (Takara - RR820A) as per manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and cDNA was synthesized according to manufacturer instructions (Takara - 6110a). 100 ng cDNA was used to check the mRNA expression of BNIP3 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by quantitative RT-PCR (qRT–qPCR) using the TB Green® Premix Ex Taq™ (Tli RNaseH Plus) (RR420A, Takara). Data were normalized to GAPDH expression utilizing the 2-ΔΔCT method ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription of RNA into cDNA was accomplished with the PrimeScript RT reagent Kit with gDNA Eraser (RR047A, Takara), followed by quantitative RT-PCR (qRT–qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed with a SYBR Premix Ex Taq II Kit (Takara, Japan) by a Real-Time System (CFX96 ...
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bioRxiv - Cancer Biology 2024Quote: ... the RNA Isolation Kit (RNAiso Plus, Takara, Japan) was employed ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed with PrimeScriptTM RT reagent Kit (Takara #RR037B), and quantitative assays were performed in triplicate using SYBR Premix Ex TaqTM II (Takara #RR820A ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... or media with Tet System Approved FBS (Takara Bio cat# 631107; Mountain View, USA) with and without doxycycline hyclate for 72 h and lysed in RIPA buffer (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using SMART-Seq Stranded Kit (Takara Bio). The quality ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantitative assays were performed in triplicate using SYBR Premix Ex TaqTM II (Takara #RR820A) in Bio-Rad iQ5 system ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed using TB Green Premix Ex Taq ™ II (Tli RNaseH Plus) (Takara) with qTOWER3 (Jena) ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse-transcription-quantitative PCR (RT-qPCR) was conducted using the Mir-X miRNA qRT-PCR TB Green Kit (TAKARA), the reverse-transcription samples ...
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bioRxiv - Cancer Biology 2024Quote: ... The Mir-X miRNA First-Strand Synthesis Kit (TAKARA, Shiga, Japan) was used to convert RNAs into complementary DNA (cDNA) ...
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bioRxiv - Cancer Biology 2024Quote: ... passed through 0.45µm filter and concentrated with RetroX Concentrator (Takara Bio USA, Mountainview, CA). Concentrated lentivirus was added to cells in the presence of 6 μg/ml polybrene (MilliporeSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... passed through 0.45um filter and concentrated with LentiX Concentrator (Takara Bio USA, Mountainview, CA). Concentrated lentivirus was added to cells in the presence of 6 μg/ml polybrene (MilliporeSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... H1299 cells were transfected with ten µg of pEGFP-N1 vector (Clontech), containing a C-terminus EGFP-tagged sequence of the full-length M237I p53 protein (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... The full-length cDNA of the human ZNRF3 was acquired from the human CRC DLD-1 cell line by RNA purification using NucleoSpin RNA II kit (Macherey Nagel) and reverse transcription RT-PCR using specific primers and Primescript RT reagent kit (TaKaRa). Then the ORF of the ZNRF3 short variant replaced RNF43 and was assembled into the C-terminal FLAG-tagged vector using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using a NucleoSpin RNA kit (Macherey Nagel) and reverse transcribed with the Primescript RT reagent kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... approximately 20% confluent HEK293T cells seeded in 12-wells plates were transfected with 300ng ZNRF3 encoding plasmid and 50ng GFP expression plasmid (pEGFP-C1, Clontech) using Lipofectamine 2000 ...
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bioRxiv - Cancer Biology 2024Quote: ... we generated lentivirus using Lenti-XTM Tet-OneTM system (Takara). To induce overexpression of YTHDC1 wild-type and mutant ...