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Citations for Takara Bio :
551 - 600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... the full-length ORF of PpGL2 fused with the GAL4 DNA binding domain in the PGBKT7 vector (Clontech, Japan) was transformed into the competent yeast strain Y2HGold ...
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bioRxiv - Plant Biology 2024Quote: ... the full-length ORF of PpGL2 was fused with the GAL4 transcriptional activation domain in the pGADT7 vector (Clontech, Japan), and then co-transformed with the pHIS2 cis reporter vector (containing triple tandem CAAATTG repeat sequence ...
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bioRxiv - Plant Biology 2024Quote: ... DNA polymerase I was purchased from Takara (Dalian, China). Key instruments and equipment include the PDS-1000 desktop gene gun (Bio Rad ...
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bioRxiv - Plant Biology 2024Quote: ... The pMD18-T vector was procured from TaKaRa Bioengineering (Dalian ...
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bioRxiv - Plant Biology 2024Quote: ... a genomic DNA walk was carried out using the Universal Genome Walker 2.0 Kit (Takara Bio Inc. Company, USA). Genomic DNA was isolated using DNeasy ® Plant Mini Kit (Qiagen) ...
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bioRxiv - Plant Biology 2024Quote: ... SYBR Premix Ex Taq II (Takara) and the primers listed in Supplementary Table 1 ...
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bioRxiv - Neuroscience 2024Quote: ... mCherry of pmCherry-C1 plasmid (Clontech, Mountain View, CA, USA) was subcloned into pAAV-hSyn-EGFP (#50465 ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells in 24-well plates were transfected with a total of 0.9 μg of DNA vectors carrying genes encoding GluN1 and GluN2 subunits and green fluorescent protein (GFP; pQBI 25 vector; for identification of transfected cells; Takara) in a 1:1:1 ratio using 0.9 μl of PolyMag reagent (OZBiosciences ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-DsRed (1:500; 632496, Clontech) and chicken anti-eGFP (to detect eYFP ...
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bioRxiv - Neuroscience 2024Quote: ... The target genes were amplified by qPCR according to the instructions of kits (Cat.no. 638315 Takara, Japan), reversely ...
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bioRxiv - Neuroscience 2024Quote: ... The cDNA was obtained by using the PrimeScript RT Reagent Perfect Real Time kit (Takara, Japan). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... The titer of AAV was determined with a real-time PCR thermal cycler (Dice, Takara Bio). The resultant AAV1-Syn-mCherry (2.8 × 1012 GC/ml) ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using TB Green Premix Ex Taq II (Takara Bio) and analysed in a Thermal Cycler Dice Real Time System Lite (Takara Bio) ...
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bioRxiv - Neuroscience 2024Quote: ... and analysed in a Thermal Cycler Dice Real Time System Lite (Takara Bio). The PCR primers used in the present study are listed in the Supplementary Table 4.
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bioRxiv - Neuroscience 2024Quote: ... The cDNA templates were synthesized from the purified RNA using the PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio, Kusatsu, Japan) with oligo (dT)20 primers ...
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bioRxiv - Neuroscience 2024Quote: ... Low input RNA-Seq library preparation (Takara SMART-Seq v4) and sequencing by Illumina NovaSeq S2 PE150 Sequencing Lane (40M read pairs/sample avg ...
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bioRxiv - Neuroscience 2024Quote: ... qRT-PCR was carried out with TB Green Premix Ex Taq II (Takara: RR82LR) using gene-specific primers ...
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bioRxiv - Neuroscience 2024Quote: ... and then the tip of the recording electrode was broken into the nested PCR reagent system (Prime ScriptTm RT reagent Kit with QDNA Eraser, Cat: RR0471, Takara), and a cDNA synthesis kit was used to synthesize cDNA following the manufacturer’s protocol (PrimeScriptm II1st Strand cDNA Synthesis Kit) ...
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bioRxiv - Neuroscience 2024Quote: Human embryonic kidney 293T cells (#632273, Takara Bio) were used to produce AAVs ...
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bioRxiv - Neuroscience 2024Quote: ... They were transfected with pAAV, pAAV2/1 (Penn Vector Core, Philadelphia, PA, USA) and pHelper plasmids (Takara Bio)52 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA (500 ng) was reverse transcribed to cDNA using the PrimeScript™ RT Reagent Kit with gDNA Eraser (Takara, RR047A). qPCR was carried out using PowerUp™ SYBR™ Green Master Mix kit and detected by QuantStudio3 system (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: Total RNAs from scramble shRNA or TDP-43 shRNA treated cells were used to construct RNA-sequencing libraries using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit (Takara 634411), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and Doxycycline Hydrochloride (1ug/ml, 631311, Clontech). Plates are fixed in 4%PFA ...
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bioRxiv - Neuroscience 2024Quote: ... and Doxycycline Hydrochloride (1ug/ml, 631311, Clontech). Progenitor cells were cryopreserved for future use in micronic vials in CryoStorCS10 (07930 ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time PCR was performed using a Thermal Cycler Dice Real-Time System II TP900 (Takara Bio, Shiga, Japan), and the relative copy number of various gene transcripts per beta-actin transcript was determined using calibration standards for each tested molecule ...
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bioRxiv - Neuroscience 2024Quote: ... Protamine sulfate (Final concentration: 8 μg/ml, Takara Bio) was added ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a T7 endonuclease I (T7EI) assay using the Guide-it Mutation Detection Kit (Takara Bio) following the standard protocol for mismatch detection.
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bioRxiv - Neuroscience 2024Quote: ... and the “SMARTer RNA Unique Dual Index Kit - 24U” (Takara Bio, Cat. No. 634451), following “SMARTer Stranded Total RNA-seq Kit v3 – Pico Input Mammalian User Manual” ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using PrimeScript™ RT Master Mix (Takara Biotechnology, Japan; RR036A),and a reaction mix was prepared using SYBR green master mix (Yeasen ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from tissues or cells using RNAiso Plus (Takara Biotechnology, Japan; 9109) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from M-exos and HM-exos using the Trizol reagent (Takara Biotechnology, Japan; 9109), and RNA integrity was assessed using a 2100 bioanalyzer (Agilent Technologies ...
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bioRxiv - Neuroscience 2024Quote: Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US). Plasmids expressing red fluorescent protein mRuby341 at 25–100 ng/µL under CAG promoter in combination with ASAP328 voltage sensor at 30–100 ng/µL under either CAG ...
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bioRxiv - Neuroscience 2024Quote: ... 1μg of total RNA was reverse transcribed using an oligo-dT primer and PrimeScript RTase (TaKaRa (Japan), 2680A) ...
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bioRxiv - Neuroscience 2024Quote: ... with TB Green Premix Ex Taq (Takara, RR420A) The specific primers used were provided in the Supplementary Table 1 ...
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bioRxiv - Plant Biology 2024Quote: ... 10 µL SYBR Premix Ex Taq II (Takara, China), 1 µL (200 nM final concentration ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using SYBR® Premix Ex Taq™ II (Perfect Real Time, Takara Bio Inc.). SYBR green detection of PCR products was conducted in real time using a MyiQ single-color detection system (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... fragmented RNA library was mixed with 5 µM of PE2-N6 primer (Supplementary Table S1) and reverse transcribed using PrimeScript RTase (Takara, SD0418) for 60Lmin at 42L°C ...
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bioRxiv - Neuroscience 2024Quote: ... and then further subcloned into pDsRed-Express2 (Clontech, cat no 632535). Due to the GC-rich region being close to the Sox2 TSS ...
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bioRxiv - Neuroscience 2024Quote: ... and rabbit anti-dsRed (Takara Bio 632393; 1:200). Then ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized with isoflurane and perfused intracardially with 1X Phosphate Buffered Saline (PBS) (Takara, Japan) and 4% Paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted as described for RNA sequencing and cDNA was generated using the PrimeScript RT reagent kit (Takara, cat. #RR037A) using a mix of oligo dT and random hexamer primers ...
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bioRxiv - Neuroscience 2024Quote: ... samples in homogenization buffer (Clontech, Cat: 2313A), were thawed on ice ...
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bioRxiv - Neuroscience 2024Quote: The primary human fibroblasts (HFs) and the corresponding iPSCs were purchased from Takara Bio (Kusatsu ...
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bioRxiv - Neuroscience 2024Quote: ... or In-Fusion cloning (Takara). The sequence of the constructs was confirmed by Sanger sequencing.
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bioRxiv - Microbiology 2024Quote: ... and then the viral DNA and the cDNA were processed with Klenow polymerase (Takara, Japan) in the presence of FR26RV-N ...
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bioRxiv - Microbiology 2024Quote: ... RNA and DNA were extracted using MiniBEST viral RNA/DNA extraction kit (Takara, Japan) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... A Premix Ex Taq™ (Probe qPCR) (TaKaRa, Japan) was used to perform qPCR following manufacturer’s instructions and each reaction consisted of 1 μl DNA template ...
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bioRxiv - Neuroscience 2024Quote: eGFP: EGFP (pAcGFP1-N1, Clontech)
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bioRxiv - Neuroscience 2024Quote: EGFP derived from pEGFP-C1 (Clontech) was cloned in-frame to the N-terminal domain of the mouse NFL gene (derived from pmNFL ...
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bioRxiv - Neuroscience 2024Quote: ... 0.02% Tween 20) and resuspend with Capture mix (50µl per ROI_index: 2xBWT, 0.5U/µl RRI (Takara)) ...