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Citations for Takara Bio :
501 - 550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... and IV from M13cp following PCR amplification and ligation using the In-Fusion Snap Assembly Master Mix (Takara Bio, 638944). The resulting helper plasmids were transformed into DH5α competent cells (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... The resulting double-stranded cDNA’s were further processed to DNA sequencing libraries using ThruPLEX DNA-seq 12S Kit (R400428, Clontech Laboratories). Libraries were size-selected by gel purification for an average size of 350bp ...
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bioRxiv - Developmental Biology 2024Quote: ... with eGFP (Clontech) and mKate2 (Evrogen ...
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bioRxiv - Plant Biology 2024Quote: ... Bait and prey constructs were transformed into the haploid yeast strains Y2HGold and Y187 (Matchmaker Gold Yeast Two-Hybrid System, Clontech), respectively ...
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bioRxiv - Plant Biology 2024Quote: ... We then ran a western blot using the GFP antibody (Takara living colors EGFP monoclonal antibody JL-8 ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA library was prepared using the SMART-Seq HT kit (Clontech). Illumina indexes were added to cDNA using the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Developmental Biology 2024Quote: ... the C-terminal twin strep tag was removed by incubating with the 3C protease (Takara Bio, Japan; 1.5 unit/50 mg protein) and ran on a size-exclusion column ...
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bioRxiv - Cell Biology 2024Quote: ... libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, 634411). Single-end sequencing was performed on the Ilumina NovaSeq platform with a sequencing depth of 80 million reads and a read length of 100 bp.
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bioRxiv - Cancer Biology 2024Quote: ... sterilized and titre measured (Go-Stix, Takara). The cell lines CACO2 ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... Linearized pMK1334 vector was purified using NucleoSpin Gel and PCR clean-up kit (Takara Bio, Cat#740609.50). Top and bottom oligos of non-targeting and targeting sgRNAs were annealed ...
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bioRxiv - Zoology 2024Quote: ... The complementary DNA (cDNA) synthesis was achieved by using ReverTra Ace-α-(Toyobo) with random primer (Takara Bio). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... GFP (1:1,000, JL-8, Clontech), GABARAP/Atg8a (1:2,000 ...
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bioRxiv - Physiology 2024Quote: ... qPCR was performed with Thermal Cycler Dice Real-time System II (Takara Bio, Shiga, Japan) and Luna Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... Both PHI-1 ORF and CESAs ORFs were amplified from first-strand DNA with PrimeScriptRTase (TaKaRa) using primers indicated in Supplemental information 4 ...
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bioRxiv - Plant Biology 2024Quote: ... with the SYBER® Premix Ex Taq TM II kit (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... coli and verified the expression cassette by sequencing and repeated again this yeast two hybrid assay by using the verified plasmid DNA instead of the corresponding PCR fragment inserts and linear vectors for mbSUS interaction tests as described “mbSUS tests protocol” (Clontech).
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bioRxiv - Plant Biology 2024Quote: ... First strands were synthesized using PrimeScript® 1st Strand cDNA Synthesis Kit (TaKaRa). RT-PCR was used to test for the presence of transcripts ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were transduced with the above-mentioned γ-retroviral SFG construct following plating on retronectin (Takara Bio)-coated plates and spinoculation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNAs were reverse-transcribed into cDNAs using cDNA Synthesis SuperMix (TaKaRa, Kusatsu, Japan), which were performed RT-qPCR using SYBR Green SuperMix (TOYOBO ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HEK 293T cells (Clontech, Mountain View, CA, USA) were grown in 15 cm dish to 60%–70% confluency ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... with the PCR product of the W56-EGFP-Linker-Synapsin1a region from pAAV-hSyn1-W56-EGFP-Linker-Synapsin1a (FWD and REV primers: 5’-GGCGCGCCCTAGAATTTCAGTCGGAGAAGAGGCTGGC-3’ and 5’-ATGCTAGGCCACCATGATGGTGGACGGCAAGCCC-3’, respectively) using In-Fusion cloning (TaKaRa Bio). pAAV-hSyn1-DIO-Scr-EGFP-Linker-Synapsin1a was generated by replacing the TurboID region of pAAV-hSyn1-DIO-TurboID (EcoRI/NcoI sites ...
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bioRxiv - Plant Biology 2024Quote: ... 1.6 μL dNTP (TAKARA, Cat. No. 4030), 0.32 μL DIG-11-dUTP (25 nmol ...
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bioRxiv - Plant Biology 2024Quote: ... 2 μg of purified total RNA was treated with RNase-free DNaseI (Takara, Dalian, China) to remove contaminated genomic DNA ...
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bioRxiv - Plant Biology 2024Quote: ... All PCR reactions were performed using the PCR components from Takara bio Inc ...
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bioRxiv - Plant Biology 2024Quote: ... the AtTPT gene was first amplified by PCR and then introduced into the pMD19-T vector (TaKaRa, Dalian, China) to construct pMD19-T-AtTPT ...
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bioRxiv - Plant Biology 2024Quote: ... The size ranges from 27 nt to 30 nt was cut and thus obtained RNA fragments were subjected into library generation using Smarter smRNA-Seq kit (Takara Cat# 635031).
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bioRxiv - Plant Biology 2024Quote: ... were enriched from the lysate by immunoprecipitation using GFP-Trap (Lablead, GNM-25-1000) and were partially digested by micrococcal nuclease (2 × 10−5 U/μL, Takara, 2910A). The digested RNA was ligated to the 3′-RNA adaptor labeled by biotin ...
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bioRxiv - Plant Biology 2024Quote: ... into chemically competent Saccharomyces cerevisiae Y2HGold cells (Takara Bio USA). Following transformation ...
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bioRxiv - Plant Biology 2024Quote: We utilized the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA) to investigate the interactions between HMA domain-containing proteins and Pwl2 ...
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bioRxiv - Plant Biology 2024Quote: ... were diluted by a 10x series dilution and spotted onto –L/-T/-H medium (Clontech) to determine interactions between the two proteins.
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bioRxiv - Physiology 2024Quote: ... 10 μM Reverse primer and TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara, #RR820W). Real-Time PCR was set up in a 384-well plate and run in a CFX384 Real-Time PCR System (BioRad ...
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bioRxiv - Physiology 2024Quote: cDNA synthesis and Real-Time PCR were performed following the manufacture instructions of the PrimeScript RT reagent Kit with gDNA Eraser kit (Takara, #RR047A). For the Real-Time PCR reaction ...
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bioRxiv - Physiology 2024Quote: ... Tissues were then incubated in a primary antibody solution (1:500 chicken anti-GFP, abcam, and 1:500 rabbit anti-mCherry, Takara) for two days at 4°C ...
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bioRxiv - Physiology 2024Quote: ... cDNAs were subcloned into pGEMHE (22) using the In-Fusion Snap Assembly Master Mix (Takara Bio, Shiga, Japan) with gene-specific primers and 15-bp sequences complementary to the ends of the linearized pGEMHE (Table S4 ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were then run on a 4-12% Bis-Tris gel and transferred to a membrane for western blotting using α-GFP (Clontech) and α-HA (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2024Quote: ... the relevant combinations of constructs were co-transformed into the auxotrophic yeast strain AH109 using the polyethylene glycol/lithium acetate method according to the manufacturer’s manual (Clontech). Yeast cells were dotted on the plates of double (-Leu-Trp ...
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bioRxiv - Physiology 2024Quote: RNA from tissues was extracted using RNAiso Plus (Takara Bio) and reverse-transcribed using ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO) ...
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bioRxiv - Physiology 2024Quote: ... Synthesized cDNA samples were used as templates for quantitative PCR using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a Thermal Cycler Dice Real Time System (Takara Bio). The amount of target RNA was normalized to the endogenous control ribosomal protein 49 gene (rp49 ...
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bioRxiv - Physiology 2024Quote: Approximately 70-80% confluent HEK293T cells (AAVpro® 293T Cell Line; Takara # 632273 AAVpro® 293T Cell Line; Takara # 632273) in DMEM (SH30022.01 ...
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bioRxiv - Physiology 2024Quote: Approximately 70-80% confluent HEK293T cells (AAVpro® 293T Cell Line; Takara # 632273 AAVpro® 293T Cell Line ...
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bioRxiv - Physiology 2024Quote: ... DRGs were transferred to a primary antibody solution (1:500 chicken anti-GFP, abcam, and 1:500 rabbit anti-mCherry, Takara) overnight ...
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bioRxiv - Physiology 2024Quote: ... Each half-site of the responsible ERE-like sequence was mutated into a HindIII recognition site using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Shiga, Japan). Transcriptional activity assays with these constructs were done as described above ...
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bioRxiv - Physiology 2024Quote: ... Three volumes of concentrated virus medium were subsequently mixed with one volume of Lenti-X concentrator (Takara Bio) and rotated at 4°C overnight before centrifugation (1500 g for 45 min at 4°C) ...
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bioRxiv - Physiology 2024Quote: ... Virus concentration was determined using Lenti-X GoStix Plus (Takara Bio) after one freeze-thaw cycle to be consistent with experimental conditions.
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bioRxiv - Plant Biology 2024Quote: ... and introduced into linearized (with KpnI) pCGEN (Motteram et al. 2011) using the In-Fusion HD Cloning Kit (Takara Bio). The resulting vectors ...
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bioRxiv - Plant Biology 2024Quote: ... coli HST08 (Stellar competent cells, Takara Bio). Verified plasmids using Sanger sequencing were electroporated into A ...
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bioRxiv - Plant Biology 2024Quote: ... vectors (Clontech) served as the backbone for generating constructs in the yeast two-hybrid (Y2H ...
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bioRxiv - Neuroscience 2024Quote: ... was purchased from Clontech (Clontech; #P3070-5). Plasmids were prepared using the ZymoPURE Plasmid MaxiPrep Kit (Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: qPCR was performed on a Thermal Cycler Dice Real Time System II (TaKaRa) with THUNDERBIRD SYBR qPCR Mix or Next SYBR qPCR Mix (TOYOBO ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviral titers were measured using Lenti-X GoStix (PT5185-2; Clontech, Takara Bio) following instructions provided by the manufacturer ...