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Citations for Takara Bio :
501 - 550 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US). Plasmids expressing red fluorescent protein mRuby341 at 25–100 ng/µL under CAG promoter in combination with ASAP328 voltage sensor at 30–100 ng/µL under either CAG ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using SYBR® Premix Ex Taq™ II (Perfect Real Time, Takara Bio Inc.). SYBR green detection of PCR products was conducted in real time using a MyiQ single-color detection system (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... fragmented RNA library was mixed with 5 µM of PE2-N6 primer (Supplementary Table S1) and reverse transcribed using PrimeScript RTase (Takara, SD0418) for 60Lmin at 42L°C ...
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bioRxiv - Neuroscience 2024Quote: ... and then further subcloned into pDsRed-Express2 (Clontech, cat no 632535). Due to the GC-rich region being close to the Sox2 TSS ...
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bioRxiv - Neuroscience 2024Quote: ... and rabbit anti-dsRed (Takara Bio 632393; 1:200). Then ...
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bioRxiv - Neuroscience 2024Quote: Mice were anesthetized with isoflurane and perfused intracardially with 1X Phosphate Buffered Saline (PBS) (Takara, Japan) and 4% Paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted as described for RNA sequencing and cDNA was generated using the PrimeScript RT reagent kit (Takara, cat. #RR037A) using a mix of oligo dT and random hexamer primers ...
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bioRxiv - Neuroscience 2024Quote: ... samples in homogenization buffer (Clontech, Cat: 2313A), were thawed on ice ...
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bioRxiv - Neuroscience 2024Quote: The primary human fibroblasts (HFs) and the corresponding iPSCs were purchased from Takara Bio (Kusatsu ...
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bioRxiv - Neuroscience 2024Quote: ... or In-Fusion cloning (Takara). The sequence of the constructs was confirmed by Sanger sequencing.
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bioRxiv - Microbiology 2024Quote: ... and then the viral DNA and the cDNA were processed with Klenow polymerase (Takara, Japan) in the presence of FR26RV-N ...
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bioRxiv - Microbiology 2024Quote: ... RNA and DNA were extracted using MiniBEST viral RNA/DNA extraction kit (Takara, Japan) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... A Premix Ex Taq™ (Probe qPCR) (TaKaRa, Japan) was used to perform qPCR following manufacturer’s instructions and each reaction consisted of 1 μl DNA template ...
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bioRxiv - Neuroscience 2024Quote: eGFP: EGFP (pAcGFP1-N1, Clontech)
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bioRxiv - Neuroscience 2024Quote: EGFP derived from pEGFP-C1 (Clontech) was cloned in-frame to the N-terminal domain of the mouse NFL gene (derived from pmNFL ...
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bioRxiv - Neuroscience 2024Quote: ... 0.02% Tween 20) and resuspend with Capture mix (50µl per ROI_index: 2xBWT, 0.5U/µl RRI (Takara)) ...
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bioRxiv - Neuroscience 2024Quote: ... and resuspended in PBS with 0.3%BSA and 1% recombinant RNase inhibitor (RRI, Takara Bio). Next ...
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bioRxiv - Neuroscience 2024Quote: ... The tdTomato-tagged Tmem120a construct was generated by PCR cloning Tmem120a using the Origene MR205146 clone as a template and subcloning it to the ptdTomato-N1 vector (Clontech), placing the tdTomato tag to the C-terminus of Tmem120a8 ...
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bioRxiv - Neuroscience 2024Quote: The mammalian expression vector pBApo-CMV-Pur (cat. #3421) was purchased from Takara. We used Genescript to insert the 144 amino acid sequence for WT synuclein into the backbone and create 6 mutated strains ...
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bioRxiv - Neuroscience 2024Quote: Media was collected 48 h after transfection and clarified by syringe filtration through a 0.45 µm polyvinylidene difluoride membrane (Millex-HV) before concentrating the virus with a Lenti-X concentrator (Clontech 631231). The concentrated virus was resuspended in PBS and aliquoted into single-use tubes stored at −80 °C ...
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bioRxiv - Pathology 2024Quote: ... SYBR Green PCR reagent (Takara Co. Ltd., Japan) was chosen for real-time fluorescence quantification ...
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bioRxiv - Cell Biology 2024Quote: RNA sequencing libraries of rRNA-depleted nuclear RNA or polyA RNA were prepared with SMARTer Stranded Total RNA-Seq Kit v2 (Takara) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... pelleted overnight using Retro-X-Concentrator (Takara Bio, 631456) and resuspended in complete TexMACS the next morning ...
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bioRxiv - Cancer Biology 2024Quote: ... Meanwhile, untreated 6-well cell culture plates (Falcon, 351146) were coated with 15 µg/ml retronectin solution (Takara Bio, T100A) overnight at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The JMJ27-GFP fusion protein was detected using the anti-GFP (Takara: 632593) 1:2,000 (v ...
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bioRxiv - Plant Biology 2024Quote: ... S1 and P1 fractions were loaded in denaturing buffer for SDS-PAGE separation and immunoblotted with the indicated antibodies (anti-DDB2: Molinier et al., 2008; anti-GFP: Takara-632593 ...
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bioRxiv - Developmental Biology 2024Quote: ... and cloned via In-Fusion HD cloning (Clontech #Z9648N) into a mo dified pHR’SIN:CSW vector under an EF1α promoter.
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bioRxiv - Cell Biology 2024Quote: ... This was then concentrated using Lenti-X concentrator (Clontech Cat. No. 631231). 5 shBLTP2 viral pellets were mixed into an shRNA cocktail in 1ml complete DMEM medium ...
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bioRxiv - Plant Biology 2024Quote: ... The PCR reaction was performed using PrimeSTAR Max DNA Polymerase (Takara Bio., Japan) with the following thermal cycling parameters ...
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bioRxiv - Neuroscience 2024Quote: ... The untagged version was made by cloning the RBP2 cDNA from this plasmid and replacing RIBEYE-GFP in the pAAV-CMV-HBA-RIBEYE-GFP-WPRE plasmid by in-fusion cloning (Takara Bio USA, Inc.).
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bioRxiv - Pathology 2024Quote: ... FAP and β-actin mRNA expression were determined using TB Green Premix Taq (Takara Bio). The primers used for β-actin were as follows ...
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bioRxiv - Pathology 2024Quote: ... and genomic DNA was removed using DNase I (TaKara). Then RNA quality was determined using Agilent 2100 BioAnalyzer (Agilent Technologies ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HEK 293T cells (Clontech, Mountain View, CA, USA) were grown in 15 cm dish to 60%–70% confluency ...
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bioRxiv - Pathology 2024Quote: ... following the manufacturer’s instructions and used for reverse transcription with the PrimeScriptTM RT reagent Kit (TaKaRa).The sequences of FaMyo5 motor domains were amplified from the cDNAs of all the F ...
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bioRxiv - Physiology 2024Quote: ... Each half-site of the responsible ERE-like sequence was mutated into a HindIII recognition site using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Shiga, Japan). Transcriptional activity assays with these constructs were done as described above ...
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bioRxiv - Physiology 2024Quote: ... Synthesized cDNA samples were used as templates for quantitative PCR using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a Thermal Cycler Dice Real Time System (Takara Bio). The amount of target RNA was normalized to the endogenous control ribosomal protein 49 gene (rp49 ...
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bioRxiv - Physiology 2024Quote: Approximately 70-80% confluent HEK293T cells (AAVpro® 293T Cell Line; Takara # 632273 AAVpro® 293T Cell Line; Takara # 632273) in DMEM (SH30022.01 ...
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bioRxiv - Physiology 2024Quote: Approximately 70-80% confluent HEK293T cells (AAVpro® 293T Cell Line; Takara # 632273 AAVpro® 293T Cell Line ...
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bioRxiv - Plant Biology 2024Quote: ... and introduced into linearized (with KpnI) pCGEN (Motteram et al. 2011) using the In-Fusion HD Cloning Kit (Takara Bio). The resulting vectors ...
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bioRxiv - Plant Biology 2024Quote: ... coli HST08 (Stellar competent cells, Takara Bio). Verified plasmids using Sanger sequencing were electroporated into A ...
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bioRxiv - Plant Biology 2024Quote: ... vectors (Clontech) served as the backbone for generating constructs in the yeast two-hybrid (Y2H ...
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bioRxiv - Molecular Biology 2024Quote: ... using the SMARTer smRNA kit for Illumina (Takara), with few modifications ...
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bioRxiv - Neuroscience 2024Quote: ... The Clock-DN-P2A sequence was inserted into an AAV-EF1α-DIO-EYFP or AAV-EF1α-DO-EYFP vector backbone using In-Fusion cloning (Takara Bio).
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bioRxiv - Neuroscience 2024Quote: ... 0.4 U/ul RNAse inhibitor (Takara, 2313B), 0.2 U/ul SUPERase•In (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... RNAse inhibitor (0.4 U/ul; Takara, 2313B), SUPERase•In (0.2 U/ul ...
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bioRxiv - Molecular Biology 2024Quote: ... coli DNA ligase (Takara, #2161). The ligated product was amplified with a G-quadruplex-specific common primer (NGS-1290 ...
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bioRxiv - Microbiology 2024Quote: ... The fusion protein was affinity-purified using TALON metal affinity resin (Takara Biosciences) and eluted in buffer containing 50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was generated using PrimeScript RT kit (Takara RR037A) with 100-500 ng RNA as input ...
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bioRxiv - Neuroscience 2024Quote: pCAG-XCaMP-R63 (gift from Haruhiko Bito, University of Tokyo) was transformed using Stellar Competent Cells (Takara Bio.), grown in antibiotic-treated LB broth at 37° C ...
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bioRxiv - Molecular Biology 2024Quote: ... TRIzol (TaKaRa, Japan) was utilized ...