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Citations for Takara Bio :
251 - 300 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... All PCR reactions were performed using the PCR components from Takara bio Inc ...
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bioRxiv - Plant Biology 2024Quote: ... the AtTPT gene was first amplified by PCR and then introduced into the pMD19-T vector (TaKaRa, Dalian, China) to construct pMD19-T-AtTPT ...
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bioRxiv - Plant Biology 2024Quote: ... The size ranges from 27 nt to 30 nt was cut and thus obtained RNA fragments were subjected into library generation using Smarter smRNA-Seq kit (Takara Cat# 635031).
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bioRxiv - Plant Biology 2024Quote: ... were enriched from the lysate by immunoprecipitation using GFP-Trap (Lablead, GNM-25-1000) and were partially digested by micrococcal nuclease (2 × 10−5 U/μL, Takara, 2910A). The digested RNA was ligated to the 3′-RNA adaptor labeled by biotin ...
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bioRxiv - Plant Biology 2024Quote: ... into chemically competent Saccharomyces cerevisiae Y2HGold cells (Takara Bio USA). Following transformation ...
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bioRxiv - Plant Biology 2024Quote: We utilized the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA) to investigate the interactions between HMA domain-containing proteins and Pwl2 ...
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bioRxiv - Plant Biology 2024Quote: ... were diluted by a 10x series dilution and spotted onto –L/-T/-H medium (Clontech) to determine interactions between the two proteins.
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bioRxiv - Physiology 2024Quote: ... 10 μM Reverse primer and TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara, #RR820W). Real-Time PCR was set up in a 384-well plate and run in a CFX384 Real-Time PCR System (BioRad ...
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bioRxiv - Physiology 2024Quote: cDNA synthesis and Real-Time PCR were performed following the manufacture instructions of the PrimeScript RT reagent Kit with gDNA Eraser kit (Takara, #RR047A). For the Real-Time PCR reaction ...
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bioRxiv - Physiology 2024Quote: ... cDNAs were subcloned into pGEMHE (22) using the In-Fusion Snap Assembly Master Mix (Takara Bio, Shiga, Japan) with gene-specific primers and 15-bp sequences complementary to the ends of the linearized pGEMHE (Table S4 ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were then run on a 4-12% Bis-Tris gel and transferred to a membrane for western blotting using α-GFP (Clontech) and α-HA (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2024Quote: ... the relevant combinations of constructs were co-transformed into the auxotrophic yeast strain AH109 using the polyethylene glycol/lithium acetate method according to the manufacturer’s manual (Clontech). Yeast cells were dotted on the plates of double (-Leu-Trp ...
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bioRxiv - Physiology 2024Quote: RNA from tissues was extracted using RNAiso Plus (Takara Bio) and reverse-transcribed using ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO) ...
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bioRxiv - Physiology 2024Quote: ... Synthesized cDNA samples were used as templates for quantitative PCR using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a Thermal Cycler Dice Real Time System (Takara Bio). The amount of target RNA was normalized to the endogenous control ribosomal protein 49 gene (rp49 ...
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bioRxiv - Physiology 2024Quote: Approximately 70-80% confluent HEK293T cells (AAVpro® 293T Cell Line; Takara # 632273 AAVpro® 293T Cell Line; Takara # 632273) in DMEM (SH30022.01 ...
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bioRxiv - Physiology 2024Quote: Approximately 70-80% confluent HEK293T cells (AAVpro® 293T Cell Line; Takara # 632273 AAVpro® 293T Cell Line ...
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bioRxiv - Physiology 2024Quote: ... Each half-site of the responsible ERE-like sequence was mutated into a HindIII recognition site using the PrimeSTAR Mutagenesis Basal Kit (Takara Bio, Shiga, Japan). Transcriptional activity assays with these constructs were done as described above ...
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bioRxiv - Plant Biology 2024Quote: ... and introduced into linearized (with KpnI) pCGEN (Motteram et al. 2011) using the In-Fusion HD Cloning Kit (Takara Bio). The resulting vectors ...
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bioRxiv - Plant Biology 2024Quote: ... coli HST08 (Stellar competent cells, Takara Bio). Verified plasmids using Sanger sequencing were electroporated into A ...
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bioRxiv - Plant Biology 2024Quote: ... vectors (Clontech) served as the backbone for generating constructs in the yeast two-hybrid (Y2H ...
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bioRxiv - Neuroscience 2024Quote: ... was purchased from Clontech (Clontech; #P3070-5). Plasmids were prepared using the ZymoPURE Plasmid MaxiPrep Kit (Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: qPCR was performed on a Thermal Cycler Dice Real Time System II (TaKaRa) with THUNDERBIRD SYBR qPCR Mix or Next SYBR qPCR Mix (TOYOBO ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells in 24-well plates were transfected with a total of 0.9 μg of DNA vectors carrying genes encoding GluN1 and GluN2 subunits and green fluorescent protein (GFP; pQBI 25 vector; for identification of transfected cells; Takara) in a 1:1:1 ratio using 0.9 μl of PolyMag reagent (OZBiosciences ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-DsRed (1:500; 632496, Clontech) and chicken anti-eGFP (to detect eYFP ...
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bioRxiv - Neuroscience 2024Quote: ... The target genes were amplified by qPCR according to the instructions of kits (Cat.no. 638315 Takara, Japan), reversely ...
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bioRxiv - Neuroscience 2024Quote: ... The titer of AAV was determined with a real-time PCR thermal cycler (Dice, Takara Bio). The resultant AAV1-Syn-mCherry (2.8 × 1012 GC/ml) ...
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bioRxiv - Microbiology 2024Quote: ... The resulting RNA was used as input for the SMARTer smRNA-Seq Kit for Illumina (Takara Cat# 635030). cDNA libraries were prepared according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 19.5 µl of nuclease-free water (Takara Bio), and 5 µl of the DNA template ...
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bioRxiv - Microbiology 2024Quote: ... comprising 25 µl of 2x probe qPCR mix (Takara Bio Inc., Otsu, Japan), 0.2 µl each of forward and backward primers (100 µmol l-1 each ...
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bioRxiv - Microbiology 2024Quote: ... was amplified with primers Fe-227S and Fe-204R using SYBR Premix Ex Taq II (Tli RNaseH Plus; Takara) (Table 2) ...
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bioRxiv - Microbiology 2024Quote: ... The cDNA was amplified using Premix Ex Taq (Probe qPCR; Takara) in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: Plasmids were introduced into HEK293T cells using the TransIT®-293 (Takara) reagent in six-well plates ...
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bioRxiv - Microbiology 2024Quote: HEK293T cells were transfected with expression plasmids using the TransIT-293 transfection reagent (Takara, Shiga, Japan), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... A3 encapsidation was determined by concentrating the virus containing supernatant using Retro-X Concentrator (Clontech) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... PrimeSTAR mutagenesis (Takara), and Gibson assembly ...
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bioRxiv - Microbiology 2024Quote: ... the RNA samples were treated with recombinant DNase I (TaKaRa). The cDNA was amplified using Premix Ex Taq (Probe qPCR ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using a PrimeScript II first-strand cDNA synthesis kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The EnvV2-Fca gene was amplified using primers Fe-gvm2Env-F and Fe-gvm2Env-R and detected using probe Fe-gvm2Env-P (containing 6-carboxy-fluorescein; FAM) (Takara). The internal control ...
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bioRxiv - Microbiology 2024Quote: ... the first strand primer was annealed to 1 µl of sample RNA before being extended with SMARTScribe reverse transcriptase (Clontech). After the addition of the second strand primer ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentiviruses were generated by seeding Lenti-X 293T cells (Takara Bio, Cat. No. 632180) in 6-well plates ...
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bioRxiv - Genetics 2024Quote: ... GFP-fused Actb WT and S348L in pcDNA3-GFP were amplified using KOD One (TOYOBO, Osaka, Japan) and cloned into pCSf107mT[31] using the In-Fusion HD Cloning Kit (Takara, Shiga, Japan) resulting in pCSf107mT-GFP-Actb WT and S348L ...
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bioRxiv - Genetics 2024Quote: ... This was performed using the In-Fusion HD Cloning Plus Kit (Takara Bio, Shiga, Japan), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... then digested with Proteinase K (Takara) at 55lJ overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... and DNA size marker ladders were from Takara. TRIzol reagent from Thermo Fisher Scientific was used for RNA isolation ...
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bioRxiv - Cell Biology 2024Quote: ... and complementary DNA (cDNA) was generated using a PrimeScriptTM RT Reagent Kit (Takara, Japan). Then ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from the liver tissue by using Trizol Reagent (Takara, Japan), and complementary DNA (cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed with a SYBR Premix Ex Taq II Kit (Takara, Japan) by a Real-Time System (CFX96 ...
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bioRxiv - Cell Biology 2024Quote: Total RNAs from HeLa cells (about 5 x 107 cells/10 cm dish) transfected with scrambled siRNA (si-Ctrl) or si-CDC5L were extracted using RNAiso Plus (Takara, 9109) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was isolated from HeLa cells or flies using RNAiso Plus (Takara, 9109) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... 7 μg of lentiviral vectors was mixed with a Lenti-X packaging (VSV-G) single shots tube (Clontech) in a final volume of 600 μl for 15 min before the transfection mix was added to subconfluent (80-90% ...