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Citations for Takara Bio :
251 - 300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... using the PrimeScript RT Master Mix (Takara). We then amplified cDNA in Power SYBR (Thermo ...
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bioRxiv - Plant Biology 2024Quote: ... The CtBOT6 DNA fragment and the amplified vector were then merged using the In-Fusion HD Cloning Kit (TaKaRa). Ct4 transformed with the pBin-GFP-hph-CtBOT6 vector express CtBOT6 under the TrpC promoter control ...
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bioRxiv - Plant Biology 2024Quote: ... amplified promoter region fragments are inserted with KpnI and XhoI site in pAT006-Fluc-iG5_Rluc-iFAD2_F vector using In-Fusion HD Cloning Kit (Takara). Amplified BOT6 fragment was inserted with pGWB402 using pENTR™ Directional TOPO® Cloning Kits (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: Lenti-X 293T cells (Takara, Cat # 632180) were transfected with each CAR expression plasmid and Mirus Bio™ TransIT™-Lenti Transfection Reagent (Cat # MIR6600) ...
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bioRxiv - Cancer Biology 2024Quote: ... and then lentivirus was harvested and concentrated using Lenti-X Concentrator (Takara Bio, 631232).
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bioRxiv - Zoology 2024Quote: ... RNA was extracted using Isogen-LS (Nippon Gene) and RT-qPCR was performed using the One-Step PrimeScript RT-PCR Kit (Takara Bio) on a 7500 Real-Time RT-PCR System (Applied Biosystems) ...
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bioRxiv - Biophysics 2024Quote: ... The protein was purified to homogeneity using a combination of TALON Metal Affinity Resin (Takara) and gel filtration chromatography ...
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bioRxiv - Physiology 2024Quote: ... SYBR green (TaKaRa) and ABI7500 system (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... were cloned into pGBKT7-GW and pGADT7-GW (Takara Bio) vectors through Gateway cloning ...
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bioRxiv - Plant Biology 2024Quote: ... or Prime STAR HS DNA Polymerase (TaKaRa) with standard protocols.
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bioRxiv - Plant Biology 2024Quote: ... The first-strand cDNA synthesis was performed with the Prime Script RT Master Mix kit (Takara). Quantitative real-time PCR was performed at 60°C using Takyon No ROX SYBR 2X MasterMix blue dTTP (Eurogentec ...
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bioRxiv - Plant Biology 2024Quote: Single or multiple DNA fragments were cloned into fungal transformation vector using In-Fusion HD Cloning Kit (Clontech, USA). Briefly ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR amplification was first performed with PrimeSTAR GXL Premix (Takara Bio, R051A) to attach Nextera-style adapter sequences ...
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bioRxiv - Plant Biology 2024Quote: ... or the SMART-Seq mRNA LP Kit (Takara Bio USA) (for pre-parasitic J2 library generation) ...
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bioRxiv - Plant Biology 2024Quote: ... using the In-Fusion HD Cloning Kit (Clontech) kit ...
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bioRxiv - Plant Biology 2024Quote: ... the 5 μl samples were used as input into the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA) (for parasitic J3 library generation ...
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bioRxiv - Synthetic Biology 2024Quote: ... has been acquired from Takara Bio.
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bioRxiv - Synthetic Biology 2024Quote: ... and IV from M13cp following PCR amplification and ligation using the In-Fusion Snap Assembly Master Mix (Takara Bio, 638944). The resulting helper plasmids were transformed into DH5α competent cells (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... The resulting double-stranded cDNA’s were further processed to DNA sequencing libraries using ThruPLEX DNA-seq 12S Kit (R400428, Clontech Laboratories). Libraries were size-selected by gel purification for an average size of 350bp ...
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bioRxiv - Developmental Biology 2024Quote: ... with eGFP (Clontech) and mKate2 (Evrogen ...
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bioRxiv - Plant Biology 2024Quote: ... Bait and prey constructs were transformed into the haploid yeast strains Y2HGold and Y187 (Matchmaker Gold Yeast Two-Hybrid System, Clontech), respectively ...
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bioRxiv - Plant Biology 2024Quote: ... We then ran a western blot using the GFP antibody (Takara living colors EGFP monoclonal antibody JL-8 ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA library was prepared using the SMART-Seq HT kit (Clontech). Illumina indexes were added to cDNA using the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Developmental Biology 2024Quote: ... the C-terminal twin strep tag was removed by incubating with the 3C protease (Takara Bio, Japan; 1.5 unit/50 mg protein) and ran on a size-exclusion column ...
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bioRxiv - Cell Biology 2024Quote: ... libraries were prepared using SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara, 634411). Single-end sequencing was performed on the Ilumina NovaSeq platform with a sequencing depth of 80 million reads and a read length of 100 bp.
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bioRxiv - Cancer Biology 2024Quote: ... sterilized and titre measured (Go-Stix, Takara). The cell lines CACO2 ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... Linearized pMK1334 vector was purified using NucleoSpin Gel and PCR clean-up kit (Takara Bio, Cat#740609.50). Top and bottom oligos of non-targeting and targeting sgRNAs were annealed ...
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bioRxiv - Zoology 2024Quote: ... The complementary DNA (cDNA) synthesis was achieved by using ReverTra Ace-α-(Toyobo) with random primer (Takara Bio). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... GFP (1:1,000, JL-8, Clontech), GABARAP/Atg8a (1:2,000 ...
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bioRxiv - Physiology 2024Quote: ... qPCR was performed with Thermal Cycler Dice Real-time System II (Takara Bio, Shiga, Japan) and Luna Universal qPCR Master Mix (New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... Both PHI-1 ORF and CESAs ORFs were amplified from first-strand DNA with PrimeScriptRTase (TaKaRa) using primers indicated in Supplemental information 4 ...
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bioRxiv - Plant Biology 2024Quote: ... with the SYBER® Premix Ex Taq TM II kit (TaKaRa) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... coli and verified the expression cassette by sequencing and repeated again this yeast two hybrid assay by using the verified plasmid DNA instead of the corresponding PCR fragment inserts and linear vectors for mbSUS interaction tests as described “mbSUS tests protocol” (Clontech).
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bioRxiv - Plant Biology 2024Quote: ... First strands were synthesized using PrimeScript® 1st Strand cDNA Synthesis Kit (TaKaRa). RT-PCR was used to test for the presence of transcripts ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were transduced with the above-mentioned γ-retroviral SFG construct following plating on retronectin (Takara Bio)-coated plates and spinoculation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNAs were reverse-transcribed into cDNAs using cDNA Synthesis SuperMix (TaKaRa, Kusatsu, Japan), which were performed RT-qPCR using SYBR Green SuperMix (TOYOBO ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HEK 293T cells (Clontech, Mountain View, CA, USA) were grown in 15 cm dish to 60%–70% confluency ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... with the PCR product of the W56-EGFP-Linker-Synapsin1a region from pAAV-hSyn1-W56-EGFP-Linker-Synapsin1a (FWD and REV primers: 5’-GGCGCGCCCTAGAATTTCAGTCGGAGAAGAGGCTGGC-3’ and 5’-ATGCTAGGCCACCATGATGGTGGACGGCAAGCCC-3’, respectively) using In-Fusion cloning (TaKaRa Bio). pAAV-hSyn1-DIO-Scr-EGFP-Linker-Synapsin1a was generated by replacing the TurboID region of pAAV-hSyn1-DIO-TurboID (EcoRI/NcoI sites ...
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bioRxiv - Plant Biology 2024Quote: ... 1.6 μL dNTP (TAKARA, Cat. No. 4030), 0.32 μL DIG-11-dUTP (25 nmol ...
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bioRxiv - Plant Biology 2024Quote: ... 2 μg of purified total RNA was treated with RNase-free DNaseI (Takara, Dalian, China) to remove contaminated genomic DNA ...
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bioRxiv - Plant Biology 2024Quote: ... All PCR reactions were performed using the PCR components from Takara bio Inc ...
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bioRxiv - Plant Biology 2024Quote: ... the AtTPT gene was first amplified by PCR and then introduced into the pMD19-T vector (TaKaRa, Dalian, China) to construct pMD19-T-AtTPT ...
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bioRxiv - Plant Biology 2024Quote: ... The size ranges from 27 nt to 30 nt was cut and thus obtained RNA fragments were subjected into library generation using Smarter smRNA-Seq kit (Takara Cat# 635031).
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bioRxiv - Plant Biology 2024Quote: ... were enriched from the lysate by immunoprecipitation using GFP-Trap (Lablead, GNM-25-1000) and were partially digested by micrococcal nuclease (2 × 10−5 U/μL, Takara, 2910A). The digested RNA was ligated to the 3′-RNA adaptor labeled by biotin ...
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bioRxiv - Plant Biology 2024Quote: ... into chemically competent Saccharomyces cerevisiae Y2HGold cells (Takara Bio USA). Following transformation ...
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bioRxiv - Plant Biology 2024Quote: We utilized the Matchmaker Gold Yeast Two-Hybrid System (Takara Bio USA) to investigate the interactions between HMA domain-containing proteins and Pwl2 ...
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bioRxiv - Plant Biology 2024Quote: ... were diluted by a 10x series dilution and spotted onto –L/-T/-H medium (Clontech) to determine interactions between the two proteins.
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bioRxiv - Physiology 2024Quote: ... 10 μM Reverse primer and TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara, #RR820W). Real-Time PCR was set up in a 384-well plate and run in a CFX384 Real-Time PCR System (BioRad ...
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bioRxiv - Physiology 2024Quote: cDNA synthesis and Real-Time PCR were performed following the manufacture instructions of the PrimeScript RT reagent Kit with gDNA Eraser kit (Takara, #RR047A). For the Real-Time PCR reaction ...
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bioRxiv - Physiology 2024Quote: ... Tissues were then incubated in a primary antibody solution (1:500 chicken anti-GFP, abcam, and 1:500 rabbit anti-mCherry, Takara) for two days at 4°C ...