Labshake search
Citations for Takara Bio :
251 - 300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Developing glutamatergic neurons were transfected with peGFP-N1 (BD Biosciences Clontech) using a calcium phosphate transfection approach ...
-
bioRxiv - Plant Biology 2024Quote: ... equilibrated with 2 μg of anti-GFP antibody (Takara Bio, 632381). Beads were washed for 2 x 10 mins in low salt wash buffer ...
-
bioRxiv - Neuroscience 2024Quote: ... Libraries were prepared using the low-input Clontech SMART-Seq HT with Nxt HT kit (Takara Bio USA) and sequenced using an Illumina NovaSeq 6000 with an S1 100 Cycle Flow Cell (v1.5).
-
The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... RNA was reverse-transcribed to cDNA by using DNA removal and a cDNA synthesis kit (PrimeScriptTM RT reagent Kit with gDNA Eraser; TaKaRa, Kusatsu, Japan) with a mixture of oligo-dT and random primers ...
-
The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germinationbioRxiv - Plant Biology 2024Quote: ... Quantification of transcript was done by qRT-PCR with fluorescent-labelled nucleotide substrate (TB Green™ Premix Ex Taq™ II, Takara or PowerUp SYBR Green Master Mix ...
-
bioRxiv - Plant Biology 2024Quote: ... complete sequences of the three genomic segments of AV2 were obtained by 5ʹ- and 3ʹ-RACE with the SMARTer RACE cDNA amplification kit (Clontech) using infected Nicotiana occidentalis leaf material (DSMZ ...
-
bioRxiv - Plant Biology 2024Quote: ... following the manufacturer’s instructions with TB Green Premix Ex Taq II (Takara, Shiga, Japan) and the primers listed in Supplemental Table S7.
-
bioRxiv - Plant Biology 2024Quote: ... two-hybrid assay was conducted according to the manufacturer’s instructions for the Match-maker Gold Yeast Two-Hybrid System (Clontech, USA). To generate the bait construct BD-GhCPK28 ...
-
bioRxiv - Plant Biology 2024Quote: ... and indexes from the SMARTer DNA HT Dual Index kit (Takara Bio). DNA fragments were then selected based on size using AMPure beds (Beckman Coulter) ...
-
bioRxiv - Plant Biology 2024Quote: ... The libraries were prepared using the ThruPLEX DNA-seq kit (Takara Bio) and indexes from the SMARTer DNA HT Dual Index kit (Takara Bio) ...
-
bioRxiv - Plant Biology 2024Quote: ... and 10 μ L SYBR premium ExTaq Perfect Real Time (TaKaRa Bio), adjusted to a final volume of 20 μL with water ...
-
bioRxiv - Plant Biology 2024Quote: ... the full-length ORF of PpGL2 fused with the GAL4 DNA binding domain in the PGBKT7 vector (Clontech, Japan) was transformed into the competent yeast strain Y2HGold ...
-
bioRxiv - Plant Biology 2024Quote: ... the full-length ORF of PpGL2 was fused with the GAL4 transcriptional activation domain in the pGADT7 vector (Clontech, Japan), and then co-transformed with the pHIS2 cis reporter vector (containing triple tandem CAAATTG repeat sequence ...
-
bioRxiv - Plant Biology 2024Quote: ... DNA polymerase I was purchased from Takara (Dalian, China). Key instruments and equipment include the PDS-1000 desktop gene gun (Bio Rad ...
-
bioRxiv - Plant Biology 2024Quote: ... The pMD18-T vector was procured from TaKaRa Bioengineering (Dalian ...
-
bioRxiv - Plant Biology 2024Quote: ... a genomic DNA walk was carried out using the Universal Genome Walker 2.0 Kit (Takara Bio Inc. Company, USA). Genomic DNA was isolated using DNeasy ® Plant Mini Kit (Qiagen) ...
-
bioRxiv - Plant Biology 2024Quote: ... SYBR Premix Ex Taq II (Takara) and the primers listed in Supplementary Table 1 ...
-
bioRxiv - Neuroscience 2024Quote: ... The primary antibody used was rabbit-anti-dsRed (1:500, Living Colors DsRed pAb, RRID: AB_10013483; Takara Bio USA, Mountain View, CA, USA) to enhance mCherry-labeled rVRG axon visualization ...
-
bioRxiv - Neuroscience 2024Quote: Human embryonic kidney cells (Lenti-X 293T Cell line from Takara. Cat no. 632180) were grown in DMEM/F12 media (GIBCO ...
-
bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using PrimeScriptTM RT reagent kit (Takara, catalog no. RR037A), and qPCR analysis was done with power SYBR Green PCR master mix (Thermo Fisher ...
-
bioRxiv - Pathology 2024Quote: ... we used the PrimeScript RT Reagent Kit (Perfect Real Time; Takara Bio Inc., Shiga, Japan). Finally ...
-
bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were first incubated with ARIAD ligand at a concentration of 1 µM (AL; D/D Solubilizer; Takara) for the indicated times ...
-
bioRxiv - Synthetic Biology 2024Quote: ... viral supernatant was harvested and concentrated using the Lenti-X concentrator (Takara, #631231) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... or In-Fusion (Takara #102518) assembly with backbones that are linearized using restriction digestion ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The human EF1α promoter was sourced from pLVX-Tet3G (Clontech/Takara). Barcodes used for the TUPVs were designed by the Elledge lab34 ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated in primary antibody (Rabbit-DsRed, 1:1000 dilution, Takara) overnight at 4 ° C ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... then cDNA was synthesized according to the PrimeScript TM II 1st Strand cDNA Synthesis Kit (Takara Bio, Dalian, China). Real-time PCR analysis was performed with the LightCycler 480 SYBR Green I Master (Roche ...
-
bioRxiv - Molecular Biology 2024Quote: ... and ligated into the digested lentiCRISPRv2 backbone using the DNA ligation kit (Mighty Mix) (Takara, Cat# 6023). The ligated DNA was then transformed into One Shot Stabl3 competent cells (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in 5% Skim milk powder (Fujifilm, Cat# 190-12865) with Phosphate buffered saline with tween® (PBS-T) (Takara, Cat# T9183). The primary antibody was incubated overnight at 4°C ...
-
MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: ... And the obtained RNA was used for reverse transcription using the PrimeScript RT Enzyme Mix I kit (TaKaRa). After the reaction was completed ...
-
MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: ... and the extracted total RNA was used to remove DNA using the DNA Eraser Buffer kit (TaKaRa, Beijing, China). And the obtained RNA was used for reverse transcription using the PrimeScript RT Enzyme Mix I kit (TaKaRa) ...
-
bioRxiv - Neuroscience 2024Quote: ... rabbit anti-DsRed (1:500; Takara Bio, RRID:AB_10013483), goat anti-HRP (1:200 ...
-
bioRxiv - Plant Biology 2024Quote: ... The PCR reaction was performed using PrimeSTAR Max DNA Polymerase (Takara Bio., Japan) with the following thermal cycling parameters ...
-
bioRxiv - Plant Biology 2024Quote: ... 1000 ng of DNase-treated RNA was used in a 20 µL reaction employing oligo-dT primers and random hexamers according to the protocol provided by Takara (Cat# RR014B). The relative expression levels of the target genes were assessed via qPCR ...
-
bioRxiv - Plant Biology 2024Quote: ... The JMJ27-GFP fusion protein was detected using the anti-GFP (Takara: 632593) 1:2,000 (v ...
-
bioRxiv - Plant Biology 2024Quote: ... S1 and P1 fractions were loaded in denaturing buffer for SDS-PAGE separation and immunoblotted with the indicated antibodies (anti-DDB2: Molinier et al., 2008; anti-GFP: Takara-632593 ...
-
bioRxiv - Microbiology 2024Quote: ... M-MLV reverse transcriptase (2641A) and TB Green® Fast qPCR Mix (RR430A) were purchased from Takara (Tokyo, Japan).
-
bioRxiv - Microbiology 2024Quote: ... was amplified by ExTaq polymerase (Takara Bio) using the primers ...
-
bioRxiv - Microbiology 2024Quote: ... Ex taq polymerase (Takara bio) was used according the manufacture’s instruction.
-
bioRxiv - Genomics 2024Quote: ... First strand reverse transcription (RT) using Smartscribe Reverse Transcriptase (Clontech) and SmartSeq template switching oligo (TSO ...
-
bioRxiv - Genomics 2024Quote: RNA was acquired from Takara (Cat# 636644). Multiplexing samples was done using one of two methods ...
-
bioRxiv - Plant Biology 2024Quote: ... PrimeSTAR® GXL DNA Polymerase (Takara), or Prime STAR HS DNA Polymerase (TaKaRa ...
-
bioRxiv - Plant Biology 2024Quote: Escherichia coli StellarTM Competent Cells (Clontech) was used as the host for DNA manipulation ...
-
bioRxiv - Neuroscience 2024Quote: ... The viral titer was measured using a Lenti-X qRT‒PCR Titration Kit (#631235, TaKaRa, Shiga, Japan).
-
bioRxiv - Neuroscience 2024Quote: ... rabbit anti-DsRed (Clontech #632496, 1:500), chicken anti-GFP (Aves Lab #GFP-1020 ...
-
bioRxiv - Neuroscience 2024Quote: ... SMART-Seq v4 ultra-low RNA (Takara Bio) library preparation protocol was used to generate cDNA libraries and sequencing was performed on an Illumina HiSeq 2 instrument with 150 bp reads according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2024Quote: Single or multiple DNA fragments were cloned into binary vector using In-Fusion HD Cloning Kit (Clontech, USA). Briefly ...
-
bioRxiv - Plant Biology 2024Quote: ... and inserted into BamHI-digested pGEX-4T-3 by using In-Fusion Smart Assembly Cloning Kit (Takarabio-Clontech). Full-length OcKSL4 cDNA was amplified by RT-PCR using primers 5’-GGATCCATGGCGAATTATCCCATGGAG-3’ (forward ...
-
bioRxiv - Neuroscience 2024Quote: ... Lentivirus-containing conditioned media were centrifuged at 500 x g for 5 min at 4°C to remove cellular debris and concentrated using Takara LentiX (Takara Cat# 631231). Briefly ...
-
bioRxiv - Synthetic Biology 2024Quote: Cells were treated with Doxycycline (Clontech) and/or Shield1 (MedCehmExpress ...