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Citations for Takara Bio :
601 - 650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... or DNA ligation kit (Takara) and transformed into StellarTM competent cells (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara Bio Inc., Shiga, Japan) and gene-specific primers at a concentration of 1 μM were used ...
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bioRxiv - Microbiology 2024Quote: ... after which DNA was extracted using the NucleoSpin Microbial DNA kit (Takara Bio Inc., Shiga, Japan), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Quantitative PCR (qPCR) was conducted using a Thermal Cycler Dice Real Time System III (Takara Bio Inc., Shiga, Japan). For each 25 μL reaction ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: Capturem Co-IP kit was procured from TaKaRa (Japan). The Neuro-2a cells were transfected with pEGFP-PKM2 and cells were infected with JEV 24 hours post-transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... The ORF of HvHsfA6a was amplified and fused to GAL4 BD in the yeast vector pGBKT7 (Clontech, CA, USA), and the resulting constructs was designated as pGBKT7:HvHsfA6a ...
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bioRxiv - Immunology 2024Quote: ... and quantified using bulk RNA-seq (Psomagen) after construction of Illumina sequencing libraries using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara). Noise from low-expression transcripts was filtered ...
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bioRxiv - Immunology 2024Quote: ... SMART-Seq HT kit (Takara) was used for full-length cDNA synthesis and amplification followed by library preparation using Illumina Nextera XT kit ...
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bioRxiv - Immunology 2024Quote: ... we ran our linearized plasmid backbone and 83 bp PCR products on a 1% agarose gel and recovered each using the Gel and PCR Clean-up Kit (Takara) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The next day these cultures were pelleted and immediately processed for plasmid extraction using the Nucleospin® Xtra Maxi Plus EF (Takara).
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bioRxiv - Molecular Biology 2024Quote: ... the cDNA was synthesized by reverse transcription using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Dalian, China) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... into HEK293FT or its derivative Lenti-X 293T cells (632180, Takara). Media was replaced after 24 hours and the viral supernatant was collected through filtration (0.45 µm pore ...
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bioRxiv - Molecular Biology 2024Quote: ... We then introduced into this 303_p3E_GI_PA our previous RNAi cassette from plasmid pME-RNAi6415 using an “In-fusion (Takara)” cloning strategy ...
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bioRxiv - Molecular Biology 2024Quote: ... We conducted an In-fusion cloning reaction (In Fusion HD kit, Takara) following the manufacturer instructions to introduce the RNAi cassette into the digested intronic sequence ...
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bioRxiv - Genomics 2024Quote: DNA libraries were prepared using ThruPLEX® DNA-Seq Kit (Takara) and DNA Unique Dual Index Kit with associated protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 ul of the cDNA was used for PCR reaction using CloneAmp HiFi PCR Premix (Takara, 639298) with the following primers ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was directly loaded into a gravity column filled with 1 mL TALON resin (TaKaRa) pre-equilibrated with the binding buffer (20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... the reaction mixtures mixed with a 6×loading buffer (Takara, Beijing, China) were loaded into the gel ...
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bioRxiv - Developmental Biology 2024Quote: ... containing 10% tetracycline-free fetal calf serum (FCS) (TaKaRa Bio, 631106), 2 mM L-Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted with the NucleoSpin® RNA kit (Takara) and RNA quality was assessed by Agilent Bioanalyzer ...
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bioRxiv - Immunology 2024Quote: ... reverse transcription and PCR steps were directly performed after sorting according to the SMART-Seq® v4 Ultra® Low Input RNA Kit protocol (Takara Bio, USA). Amplified complementary DNA were transferred to the Benaroya Research Institute (Seattle ...
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bioRxiv - Immunology 2024Quote: ... of 25 μg/mL Retronectin (Takara) in PBS and incubated at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... HEK 293T cells were sourced from the American Type Culture Collection (ATCC) and GP2-293 from Takara Bio ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR-amplified enhancer-containing fragments were tandemly inserted into the upstream region of the original promoter sequence by In-Fusion cloning (TAKARA BIO, Shiga, Japan). This process is summarized in Figure S3 ...
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bioRxiv - Molecular Biology 2024Quote: ... YCplac22 plasmids that carry tsa1 mutant alleles were constructed using the PrimeSTAR Mutagenesis Basal Kit (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Lentivirus titration was determined using Lenti-X p24 Rapid Titre Kit (Takara #631476) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... After RNA Reverse transcription (Prime Script RT reagent, Takara), cDNA levels were measured by qPCR Fast SYBR™ Green Master Mix (Ref ...
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bioRxiv - Neuroscience 2024Quote: ... The preserved HEK medium was then spun down with 12.5 ml of lenti-X concentrator (Takara Bio), in an effort to capture the two distinct lentiviruses ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries of cDNA were then generated using the SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Takara). The resulting cDNA libraries were then quantified by the High Sensitivity DNA assay ...
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bioRxiv - Neuroscience 2024Quote: ... and re-plated at a concentration of 2.5×105 cells/cm2 onto plates coated with 4 μg/mL iMatrix-511 (Takara Bio) in N2B27 medium supplemented with 10 μg/mL BDNF and 10 µg/mL Rock Inhibitor Y-27632 ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit Anti-dsRed (Takara 632496, 1:1000); Goat Anti-Calb2 (Swant CG1 ...
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bioRxiv - Biophysics 2024Quote: ... was cloned from the Saccharomyces cerevisiae S288c genome into a pETM-11 expression vector linearised at the XhoI and XbaI restriction sites using In-Fusion (Takara) technology ...
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bioRxiv - Genetics 2024Quote: Long-range PCR was performed using the TaKaRa LA PCR kit (TaKaRa Bio). PCR conditions were optimized for the extension-annealing phase ...
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bioRxiv - Biochemistry 2024Quote: ... grown and concentrated using Lenti-X Concentrator (Clontech Laboratory, 631232) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from monocytes using NucleoSpin® RNA kit (Takara) and RNA concentration was measured using NanoDrop Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... The pmEOS2-C1-ERGIC53 plasmid was constructed using using the In-Fusion® Snap Assembly Cloning Kit (Takara) and transformed into OmniMAX competent cells ...
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bioRxiv - Molecular Biology 2024Quote: RNAi-Ready pSIREN-RetroQ vector (Clontech, Inc. Mountain View, CA, USA) was used to prepare shRNA constructs targeting Selenon gene (KD1 ...
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bioRxiv - Molecular Biology 2024Quote: ... These constructs were transfected into the 293 Ecopack packaging cell line (Clontech) using Lipofection 2000 transfection reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.25 µL of ExTaq (TaKaRa), 1× ExTaq buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Agarose gel electrophoresis was performed on a Mupid EX system (Takara) in 400 mL of 1× MOPS buffer at 20 V for 20 min and then at 100 V until the bromophenol blue dye had migrated approximately 2/3 of the gel ...
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bioRxiv - Neuroscience 2024Quote: ... and at 72°C for 30 sec in TaKaRa Thermal Cyclar Dice Touch (Takara).
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bioRxiv - Neuroscience 2024Quote: ... Reverse transcription was performed with 500 ng of total RNA and PrimeScript™ RT Master Mix (Perfect Real Time) (Takara, Tokyo, Japan). Reverse transcription PCR (RT-PCR ...
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bioRxiv - Neuroscience 2024Quote: ... 10mM dNTP (Takara, Japan), 0.1M DTT (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... mCherry of pmCherry-C1 plasmid (Clontech, Mountain View, CA, USA) was subcloned into pAAV-hSyn-EGFP (#50465 ...
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bioRxiv - Neuroscience 2024Quote: ... Low input RNA-Seq library preparation (Takara SMART-Seq v4) and sequencing by Illumina NovaSeq S2 PE150 Sequencing Lane (40M read pairs/sample avg ...
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bioRxiv - Neuroscience 2024Quote: ... qRT-PCR was carried out with TB Green Premix Ex Taq II (Takara: RR82LR) using gene-specific primers ...
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bioRxiv - Plant Biology 2024Quote: ... 10 µL SYBR Premix Ex Taq II (Takara, China), 1 µL (200 nM final concentration ...
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bioRxiv - Neuroscience 2024Quote: ... and the “SMARTer RNA Unique Dual Index Kit - 24U” (Takara Bio, Cat. No. 634451), following “SMARTer Stranded Total RNA-seq Kit v3 – Pico Input Mammalian User Manual” ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using PrimeScript™ RT Master Mix (Takara Biotechnology, Japan; RR036A),and a reaction mix was prepared using SYBR green master mix (Yeasen ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from tissues or cells using RNAiso Plus (Takara Biotechnology, Japan; 9109) according to the manufacturer’s guidelines ...