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Citations for Takara Bio :
4351 - 4400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Based on the pEGFP.C1 vector (Clontech) plasmids for the CMV promoter-driven expression of fusion proteins of GFP with either only the inhibitory receptor cytoplasmic domain or the joint transmembrane plus cytoplasmic domain were generated ...
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bioRxiv - Immunology 2023Quote: ... Full-length cDNAs were prepared using the SMART-Seq HT Kit (Takara Bio, Mountain View, CA) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: qRT-PCR was performed using SYBR® Premix Ex Taq™ II (Takara Biotechnology) and CFX96 Connect Real-Time system (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2023Quote: ... MA050368 (Takara Bio); Cxcl10 ...
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bioRxiv - Immunology 2023Quote: ... MA118556 (Takara Bio); Cxcl9 ...
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bioRxiv - Cell Biology 2023Quote: ... secreted embryonic alkaline phosphatase (SEAP) activity and TGF-β concentration were determined using the Great EscAPe® SEAP (631738, TaKaRa Bio, Kusatsu, Japan) chemiluminescence kit to quantify SEAP activity (excitation wavelength 360 nm ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was isolated from frozen cells using the Nucleospin Blood XL kit (Takara Bio, #740950.10) and amplified with barcoded primers by index PCR (see table S10 for sequences) ...
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bioRxiv - Immunology 2023Quote: ... Virus supernatant was harvested three days later and coated on retronectin (TaKaRa)-treated plates (incubated overnight at 4°C previously ...
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bioRxiv - Immunology 2023Quote: ... Complementary DNA was prepared using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech Laboratories, CA, USA). Libraries were prepared using a Nextera XT DNA Library Prep Kit (Illumine ...
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bioRxiv - Immunology 2023Quote: ... RNA extracted and amplified (10 ng RNA/sample) using SMART-Seq® HT kit (Takara Bio, #634455) according to the manufacture’s instruction ...
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bioRxiv - Immunology 2023Quote: ... RNA extracted and amplified (10 ng RNA/sample) using SMART-Seq® HT kit (Takara Bio, #634455) according to the manufacture’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... sgRNAs were produced by sgRNA IVT kit (Takara Bio) by following the manufacturer’s instructions and RNA was isolated by Trizol (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... at 1.6×106 cells/dish in RHB-A (Takara-Clontech), changing media every 24 h ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-dsRed (Clontech, 632496) at 1:300 ...
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bioRxiv - Microbiology 2023Quote: AH109 yeast cells (Clontech) were used for yeast two-hybrid experiments ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR-amplified donor sequences were independently cloned by In-fusion reaction (Takara) into the backbone of the Addgene plasmid 58409 which contains the homology arms (HA ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA from the total RNA was synthesized using the Advantage® RT-for-PCR kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... University of Cambridge using SMART-Seq v4 to generate the cDNA (Takara) and then adapter and index ligation was performed using the Nextera XT workflow (Illumina) ...
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bioRxiv - Plant Biology 2023Quote: ... after which first-strand cDNA was synthesized from total RNA using a cDNA reverse transcription kit (TaKaRa, Japan). Full-length CDS sequences of the three candidate UGT genes were obtained from third-generation transcriptome sequencing data (Chen et al ...
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bioRxiv - Plant Biology 2023Quote: ... The expression of marker genes was detected by qRT-PCR using the One-step TB Green PrimeScriptTM RT-PCR kit II (Takara, Osaka, Japan). All genes were normalized against the level of an actin reference gene (Foo et al. ...
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bioRxiv - Plant Biology 2023Quote: ... the fusion protein was purified using TALON® Cobalt resin (Takara Bio). Following extensive washing in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: AAV transfer plasmids were constructed by removing all the sequences between the ITRs of the pAAV-CMV vector (TaKaRa) and inserting the Mhck7 promoter ...
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bioRxiv - Cell Biology 2023Quote: ... The helper plasmid used was pHelper (TaKaRa).
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bioRxiv - Developmental Biology 2023Quote: ... dsDNAs were amplified by PCR using modified primers with 5’Bioton – 5 x phosphorothioate bonds (synthesized by eurofins) and PrimeSTAR Max (Takara).
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bioRxiv - Cell Biology 2023Quote: ... clustering was performed by heterodimerization between LAMP1-mCherryFKBP (lysomes) and BicD-FRB (dynein adaptor) by the use of Rapalog (A/C Heterodimerizer ref. n°635057, from Takara Bio Inc.). Cells were cultured in glass bottom dishes and imaged at different time points (24 ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μg of total RNA was used to make cDNA libraries using prime script RT reagent kit gDNA eraser (Takara Bio Inc.) according to the kit’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: DNA template for RNA synthesis was created using the forward and reverse primers listed below with GXL prime star PrimeSTAR® GXL DNA Polymerase (Takara Cat# R050A). mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit (ThermoFisher Cat# AM1345 ...
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bioRxiv - Developmental Biology 2023Quote: ... using In-Fusion cloning (Takara Bio, primers are listed in Table S3) and then inserted into a pDestTol2ACryGFP backbone (Berger and Currie ...
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bioRxiv - Biochemistry 2023Quote: ... by RT-PCR (Takara, RR014) and cloned by Gibson assembly into the vector pK27SUMO ...
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bioRxiv - Cell Biology 2023Quote: TurboID plasmids were made using In-Fusion Recombination (Takara Bio USA, Inc.). 3xHA-TurboID was amplified from 3xHA-TurboID-NLS pcDNA3 (Addgene plasmid #107171 ...
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bioRxiv - Cell Biology 2023Quote: ... and Terra (Takara) polymerases as well as by microscopy using RNA-FISH detecting intronic sequences (Supplementary Figure S1B ...
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bioRxiv - Cell Biology 2023Quote: ... Lenti-X 293T cells were obtained from Takara Bio and cultured in DMEM (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: Yeast two-hybrid assays were performed according to the Yeastmaker Yeast Transformation System 2 manual (Clontech). The yeast strain AH109 was co- transformed with an AD-fused and a BD-fused construct using the lithium acetate method ...
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bioRxiv - Developmental Biology 2023Quote: ... then inserted into pCS2+ vector (Clontech) fused with C-terminal HA tag ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA fragment encoding zebrafish Foxo1a was amplified by PCR and subcloned into pEGFP-C3 vectors (Takara Bio Inc.) to generate the expression plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... and staining with anti-DsRed (Takara Bio, #632496, 1/1000), anti-Pax7 (purified hybridoma ...
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bioRxiv - Cell Biology 2023Quote: ... For immunostaining the slides were incubated with primary antibodies anti-DsRed (Takara Bio, #632496, 1/1000), anti-Pax7 (purified hybridoma ...
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bioRxiv - Cell Biology 2023Quote: ... added with 0.8 ml pure TALON IMAC (Clontech) resin and 20 mM imidazole ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 0.2 mM dNTPs and 0.5 U TaKaRa rTaq DNA polymerase (TaKaRa, Otsu, Japan). The amplification was carried out by PCR involving a denaturation step at 94 °C for 3 min ...
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bioRxiv - Bioengineering 2023Quote: ... CloneAmp HiFi PCR Premix (Takara Bio USA, San Jose, CA, USA) was used for PCR amplification following the manufacturer’s instructions using the following protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Anti-GFP (monoclonal antibody raised in mouse, Takara, USA, catalog # 632381) and anti-SEC12 (Bar-Peled and Raikhel ...
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bioRxiv - Plant Biology 2023Quote: ... RNA (0.5 μg) was reverse transcribed by the PrimeScript RT reagent kit with genomic DNA Eraser (Takara, USA) using random and oligo-dT primer mix in a 10 μL reaction ...
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bioRxiv - Genomics 2023Quote: ... The total RNA library was prepared using SMARTer® Stranded Total RNA-Seq Kit v2 (TAKARA 634413). The rest of the nuclei were reverse crosslinked ...
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bioRxiv - Neuroscience 2023Quote: ... comprising a red-shifted variant GFP cDNA and a downstream polyadenylation signal derived from pIRES2-EGFP (Takara Bio), was inserted in-frame into the initiation codon of the Scn1a coding exon 1 using the Red/ET Recombineering kit (Gene Bridges) ...
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bioRxiv - Bioengineering 2023Quote: ... supernatant was loaded to a TALON met al affinity (Clontech, 635502) gravity column ...
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bioRxiv - Biochemistry 2023Quote: ... Mutagenesis and cloning were done using In-Fusion (Takara, Shiga, JP). Primers and plasmids used are listed in Table S4 ...
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bioRxiv - Biochemistry 2023Quote: ... MA) and the protein was purified using Talon beads (Takara Bio Inc., Japan) following published protocols 37,38 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with Talon beads (Takara Bio Inc., Japan) for an hour at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... 661W cells were transfected with the calcium phosphate technique or the XfectTM transfection reagent (Takara). MEF cells were transfected using TurboFectTM Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Cerulean and Myo7b blots were stained for 1 h at room temperature (RT) or overnight at 4 °C using the mouse anti-GFP (detects cerulean, 1:2000, JL-8, Clontech, Takara) and the mouse anti-β-tubulin antibody (1:500 ...