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Citations for Takara Bio :
4201 - 4250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... pBI-BFP (made by replacing AcGFP1 in pBI-CMV2 Vector (Clontech) with BFP from pTagBFP-N Vector (Evrogen ...
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bioRxiv - Biophysics 2023Quote: ... using Bsp1407I (TaKaRa, Shiga, Japan) and inserted into mCherry-C1 (mCherry-TIA1).
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The Hippo pathway terminal effector TAZ/WWTR1 mediates oxaliplatin sensitivity in colon cancer cellsbioRxiv - Cancer Biology 2023Quote: ... Resulting oligos were then annealed together and cloned into the pSIREN-RetroQ vector (TaKaRa) according to the manufacturer’s protocol between BamHI and EcoRI cloning sites.
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bioRxiv - Cell Biology 2023Quote: ... Isolated DNA was amplified by PCR using Hot Start TaKaRa LA Taq kit to yield a 10-Kb product (Takara Biotechnology, #RR042A). Primers utilized were the following ...
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bioRxiv - Neuroscience 2023Quote: ... using seamless cloning (InFusion (Takara) or HiFi (NEB)).
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bioRxiv - Cell Biology 2023Quote: ... Real-time qPCR amplification was performed using the PrimeScript™ RT-PCR Kit (Takara, Catalog#RR420). Alp ...
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bioRxiv - Cell Biology 2023Quote: ... Single-stranded cDNA was reverse transcribed from 500ng total RNA using reverse transcriptase and oligo(dT) primer (Takara, Catalog#RR036). Real-time qPCR amplification was performed using the PrimeScript™ RT-PCR Kit (Takara ...
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bioRxiv - Cancer Biology 2023Quote: ... Viruses were concentrated with lenti-X concentrator (Clontech). Cell lines to be infected with virus were seeded with 200,000 cells in each well of a 6-well plate ...
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bioRxiv - Developmental Biology 2023Quote: ... pTRE3G (TaKaRa Bio) was used as the backbone ...
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bioRxiv - Developmental Biology 2023Quote: ... The yeast two-hybrid library was generated from the poly(A)+ mRNA isolated from mixed-stage N2 nematodes using the Matchmaker kit (Clontech) as per the manufacturer’s protocol and transformed into Y1HGold strain (MATα ...
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bioRxiv - Developmental Biology 2023Quote: ... fbf-2 cDNA was cloned into the pGBTK vector (Clontech) and transformed into PJ68-4a yeast strain (MATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ ...
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bioRxiv - Bioengineering 2023Quote: ... Lentivirus was then concentrated 100X using the Lenti-X concentrator (Takara Bio,631232). Biological titration of lentivirus by QPCR was carried out as previously described86 ...
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bioRxiv - Bioengineering 2023Quote: ... 37°C for 2 hours in a plate coated with Retronectin (Takara Bio, T100B) with an MOI of ∼5.0 for each lentivirus (dCas9 lentivirus at MOI ∼5.0 and gRNA-MCP-fusion effector lentivirus) ...
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bioRxiv - Plant Biology 2023Quote: ... These plasmids were transformed into the yeast strain AH109 for testing protein-protein interaction using the Matchmaker Gold system (Takara Bio, Kusatsu, Japan). Yeast transformation and growth were performed as described in [Pecher et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... using Primestar-GXL polymerase (Takara) and the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... The full-length was also cloned into the lentiviral vector pLVX-TetOne-Puro (Clontech, Takara, #631849). The plasmid pECMV-3×FLAG-RAB11A was purchased from SinoBiological (Beijing ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HepG2 cells were resuspended in ice-cold PBS supplemented with protease inhibitors (Clontech, Mountain View, CA, USA) and lysed in ice bath by sonication in cycles of 10 s on /5 s off for 1 min at 6–10 μm amplitude at 25 % intensity from an exponential ultrasonic horn of 3 mm in a Soniprep 150 MSE (MSE Ltd. ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Inducible expression of a Nef-eGFP fusion protein was established in CEM-T4 cells using the Lenti-XTM Tet-On System (Clontech now Takara Bio USA) which places expression of Nef-eGFP under the control of a doxycycline-inducible promoter ...
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bioRxiv - Physiology 2023Quote: ... Slides were incubated for 18 hours at room temperature in anti-dsRed antibody (TaKaRa, 1:2000) in 0.1M PB containing 0.3% Triton X-100 and 1% donkey serum ...
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bioRxiv - Genetics 2023Quote: ... the sequences corresponding to Flag tags were replaced by those encoding for Myc tags using the In-Fusion PCR cloning system (Takara) according to the kit’s guidelines ...
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bioRxiv - Genetics 2023Quote: ... according to the steps in the instruction book of SYBR PrimeScript RT-PCR kit (Takara). We used 2-ΔΔc to indicate the relative gene expression level of each sample.
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bioRxiv - Cell Biology 2023Quote: ... hygromycin B (300 µg/ml) and 10% tetracycline free fetal bovine serum (Takara Bio, USA) in a humidified incubator at 37°C in 5% CO2 in 6-well plates ...
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bioRxiv - Microbiology 2023Quote: ... Thermal Cycler Dice Real Time System III (Takara), or 7500 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (ref.69) (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). On day 3 (24 hours post-transfection) ...
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bioRxiv - Microbiology 2023Quote: ... The protocol was as follows: PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (Takara Bio, Shiga, Japan), nuclease-free PCR-grade water ...
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bioRxiv - Genomics 2023Quote: ... except for gRNA cloning that was performed using In-Fusion cloning (Takara) and the drug-selection cassette of blasticidin instead of human dhfr was used ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was carried out using SmartScribe RT (Takara), and Q5 polymerase (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: cDNA was amplified from the collected cells using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-TakaraBio). 1ng of amplified cDNA was used to generate barcoded libraries with the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR was performed with ExTaq (TaKaRa, Japan) on 2720 thermal cycler (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... Full length cDNAs were generated from 350 to 1,000 pg of total RNA using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe ...
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bioRxiv - Plant Biology 2023Quote: The Matchmaker Gold Yeast 1-hybrid system (Clontech) with the yeast one-hybrid (Y1H ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA fragment was inserted into the cloning site of pColdIII expression vector (TaKaRa Bio, Shiga, Japan). The constructed vector and the chaperone co-expression plasmid pGro1254 were introduced into E ...
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bioRxiv - Plant Biology 2023Quote: ... SRF mutants were prepared using PrimeSTAR Mutagenesis Basal Kit according to the manufacturer’s instructions (TaKaRa Bio, Shiga, Japan), and the mutations were confirmed by sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... and each was cloned into NdeI/SalI sites of the pRI201-AN vector (TaKaRa Bio, Shiga, Japan) to express under the control of the 35S promoter ...
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bioRxiv - Molecular Biology 2023Quote: ... Promoterless bicistronic vectors were generated by removing the SV40 promoter from the bicistronic vector pRUF and the pRUF vectors containing the putative IRES sequences by PCR amplification using divergent primers (Supplementary Table 3) and the CloneAmp™ HiFi PCR Premix (Takara) polymerase ...
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bioRxiv - Molecular Biology 2023Quote: ... linearized by PCR and using the divergent primers reported in Supplementary Table 3 and CloneAmp™ HiFi PCR Premix (Takara) polymerase ...
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bioRxiv - Plant Biology 2023Quote: ... the resultant PCR products were inserted into the NdeI and KpnI restriction sites of the modified pColdIII expression vector (TaKaRa Bio, Shiga, Japan) containing the His6-tag sequence with stop codon (5’-catcaccatcaccatcactag-3’ ...
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bioRxiv - Plant Biology 2023Quote: ... the chimeric H5Indo gene was reamplified by PCR and then introduced in the T-DNA region of a pCAMBIA binary vector previously linearized with restriction enzymes SacII and StuI using the In-Fusion cloning system (Clontech). The same methods and PDI signal peptide were used to clone antibody chain genes ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant CML13 and CML14 were purified via Ni-NTA His60 (Takara Biosciences.) and CaM7 using phenyl-sepharose (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was transcribed into full-length complementary DNA using a SMART-Seq v4 Ultra Low Input RNA kit (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... The cDNA of each mouse gene was amplified and detected using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO) and Thermal Cycler Dice Real Time System III (TAKARA BIO, Shiga, Japan). 18S ribosomal RNA was used for the reference gene ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-dsRed (Takara Bio) 1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... coated with 1 µg/cm2 of iMatrix-511 (Takara Bio). Hypothalamic cultures were loaded with the Fluorescent Dye-Based Cal-590 following the manufacturers’ instructions (Stratech) ...
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bioRxiv - Neuroscience 2023Quote: Libraries were constructed with the SMARTer® Stranded Total RNA-Seq Kit – Pico Input Mammalian kit (Clontech Laboratories), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) using the PrimeScript cDNA Synthesis Kit (Takara). Synthesized cDNA was utilized as a template for polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2023Quote: ... LDH release was quantified using an LDH Cytotoxicity Detection Kit (Clontech) according to the manufacturer’s instructions and normalized to mock-infected (min cytotoxicity ...
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bioRxiv - Plant Biology 2023Quote: ... Two biological replicates were processed for next-generation library preparation using the ThruPLEX DNA-seq kit (Takara). Next ...
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bioRxiv - Microbiology 2023Quote: ... reverse transcription of the RNA using a primer designed to the constant region of the barcoded adaptor with addition of an adapter to the 3’ end of the cDNA by template switching using SMARTScribe (Clontech) as described (23) ...
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bioRxiv - Synthetic Biology 2023Quote: ... SD-Trp-Ura or SD-His-Leu (Clontech Takara).
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bioRxiv - Synthetic Biology 2023Quote: ... using the Advantage 2 Polymerase kit (Clontech) and specific primers located on either side of the target locus ...