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Citations for Takara Bio :
4401 - 4450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... pLVX-DsRed-Monomer-N1–hVMP1 was obtained by inserting the human VMP1 sequence (NCBI reference sequence: NM_030938.5) into pLVX-DsRed-Monomer-N1 (CLONTECH cat. 632152). pLenti–VMP1–GFP was obtained by cloning the VMP1 sequence (NCBI reference sequence ...
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bioRxiv - Developmental Biology 2023Quote: ... at 1.6×106 cells/dish in RHB-A (Takara-Clontech), changing media every 24 h ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-dsRed (Clontech, 632496) at 1:300 ...
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bioRxiv - Microbiology 2023Quote: AH109 yeast cells (Clontech) were used for yeast two-hybrid experiments ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR-amplified donor sequences were independently cloned by In-fusion reaction (Takara) into the backbone of the Addgene plasmid 58409 which contains the homology arms (HA ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA from the total RNA was synthesized using the Advantage® RT-for-PCR kit (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... University of Cambridge using SMART-Seq v4 to generate the cDNA (Takara) and then adapter and index ligation was performed using the Nextera XT workflow (Illumina) ...
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bioRxiv - Developmental Biology 2023Quote: ... sgRNAs were produced by sgRNA IVT kit (Takara Bio) by following the manufacturer’s instructions and RNA was isolated by Trizol (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: Based on the pEGFP.C1 vector (Clontech) plasmids for the CMV promoter-driven expression of fusion proteins of GFP with either only the inhibitory receptor cytoplasmic domain or the joint transmembrane plus cytoplasmic domain were generated ...
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bioRxiv - Immunology 2023Quote: ... Full-length cDNAs were prepared using the SMART-Seq HT Kit (Takara Bio, Mountain View, CA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... The DNA oligos were then ligated to PX458 vector linearized by BpiI (Takara) and cloned into E.coli DH5α competent cells (Takara) ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Plasmids were assembled from linear fragments using In-Fusion HD (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... PCR (PrimeSTAR MAX DNA polymerase, Takara Bio USA, Inc.) was performed using divergent primers flanking or spanning the junction site of the specific circRNA ...
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bioRxiv - Plant Biology 2023Quote: ... and cloned into the XbaI digestion site of pMpGWB307 vectors using In-Fusion HD Cloning Kit (Takara Bio) to produce pMpGWB307-MpJINp ...
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bioRxiv - Developmental Biology 2023Quote: ... and mRNA was captured and amplified with the Smart-seq2 v4 kit (Clontech). After AMPure XP beads purification ...
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bioRxiv - Immunology 2023Quote: ... 100 units RNAse-Inhibitors (Takara)) for 20 min at 4°C in a rotating mixer ...
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bioRxiv - Cell Biology 2023Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Cell Biology 2023Quote: ... plasmid backbones derived from EYFP-N1 (Clontech/Takara), Lenti-Cas9-blast (a kind gift of F ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was obtained by centrifugation of the cell lysate at 10,000 g for 30 minutes and applied into the His TALON™ gravity column (Clontech). The columns were washed to remove the non-target proteins ...
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bioRxiv - Cell Biology 2023Quote: ... PCR amplification was conducted using PrimeSTAR Max (Takara) or KOD-Plus-Neo (TOYOBO).
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bioRxiv - Molecular Biology 2023Quote: ... Primers and templates were mixed with TB Green Premix Ex Taq II (Takara Bio). mRNA levels were then normalized to that of Gapdh and calculated using the comparative Ct method ...
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bioRxiv - Genomics 2023Quote: ... coli using the Nucleobond XtraBAC kit (Takara 740436) and preps were stored at 4 °C.
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bioRxiv - Cell Biology 2023Quote: ... Library preparation was performed with SMART Ultra-Low Input kit v4 (Takara) and Nextera™ XT Library Prep kit (Illumina) ...
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bioRxiv - Immunology 2023Quote: ... (Cat.No. 632180) were cultured in DMEM supplemented with 10% Tet System Approved FBS (Takara, Cat.No. 631106). All cell lines were cultivated at 37°C in a humidified atmosphere with 5% CO2.
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bioRxiv - Immunology 2023Quote: ... Plasmids were packed into lentiviral particles using the Lenti-X cell line and Lenti-X Packaging Single Shots (VSV-G) (Takara, Cat.No. 631276) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... qRT-PCR was performed using SYBR Premix Ex Taq (Tli RNaseH Plus; TaKaRa, Tokyo, Japan) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Division of AIDS, NIAID, NIH (contributed by Dr. John C. Kappes, Dr. Xiaoyun Wu and Tranzyme Inc.) Lenti-X 293T cells (Takara Bio Inc.), TZM-bl cells ...
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bioRxiv - Microbiology 2023Quote: ... One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (RR096A, Takara Bio Inc.), and primer pairs for Zcchc3 (5′-CTCTCTATGCCTTCTTAAACCGA-3′ and 5′-CATCTGCACGCTACAGTTCT-3′ ...
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bioRxiv - Microbiology 2023Quote: The whole Gag sequence was amplified from wild-type Gag plasmid by PCR and cloned into pGEX6P1 and pmCherry-C1 (Takara Bio Inc.). Fragments of MAp17 (a.a ...
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bioRxiv - Microbiology 2023Quote: ... pGP (# 6161) and pDON-5 Neo DNA (# 3657) were purchased from Takara Bio Inc ...
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bioRxiv - Cell Biology 2023Quote: ... pombe genome using KOD One DNA polymerase (TaKaRa Bio Inc., Japan). The first PCR products were used as primers in the second PCR step to amplify a cassette for integration ...
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bioRxiv - Developmental Biology 2023Quote: ... or SMARTer™ RACE cDNA Amplification Kit (Clontech). A cDNA library was used as a template for PCR (Nanos1 ...
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bioRxiv - Neuroscience 2023Quote: ... The rest of the clones were linearized using restriction enzymes bought from Takara, using a standard procedure ...
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bioRxiv - Microbiology 2023Quote: The following plasmids were used in this study and previously described: enhanced green fluorescent protein (EGFP) pEGFP-N1 (Clontech Laboratories, Mountain View, CA), pCI-neo HSV-2 UL21 (UL21 ...
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bioRxiv - Microbiology 2023Quote: The 16S rRNA gene sequences were amplified by PCR using LA Taq polymerase (TaKaRa-Bio, Inc., Shiga, Japan) using the primers Uni530F and Uni907R65 ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... genomic DNA was isolated using Guide-it Mutation Detection Kit (Takara). DNA fragments flanking the targeted Drd1a locus and off-targets (Figure S4C ...
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bioRxiv - Neuroscience 2023Quote: In Fusion cloning (Takara Bio) was used to assemble the responder vector (p371 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 μM Shield1 (Clontech lab, now Fisher Scientific) for 4 h ...
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bioRxiv - Microbiology 2023Quote: ... pSIREN-shRNA-LucIRR and pSIREN-shRNA GAPDHHB were purchased from Clontech. pCDNA3.1 was purchased from Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... pEGFP-N3 was from Clontech and pGEM-T Easy was from Promega (cat# A137A) ...
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bioRxiv - Plant Biology 2023Quote: ... The double-strand oligonucleotides containing the target sequences were synthesized and cloned into pMpGE_En01 or pMpGE_En03 using In-Fusion HD cloning kit (TaKaRa) and BsaI restriction sites in pMpGE_En03 ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time PCR was performed on a thermal cycler Dice Real Time System (Takara) using KAPA SYBR FAST qPCR Kit (KAPA Biosystems ...
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bioRxiv - Plant Biology 2023Quote: ... PCR was performed by TaKaRa EX Taq (Takara) and primers ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was isolated from 14-day-old thalli of the wild type and Mpacl5 mutants grown on the half-strength B5 agar medium using NucleoSpin RNA Plant (Takara). The sequence libraries were generated by TruSeq RNA Sample Prep Kit (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... PCR was performed by TaKaRa EX Taq (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... The fragment was then excised by XbaI and BamHI and inserted into the downstream of the CaMV35S promoter of pBI121 (Takara) whose GUS reporter gene was removed in advance to generate the CaMV35S:MpACL5 construct ...
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bioRxiv - Plant Biology 2023Quote: The internal fragments of the target genes were amplified by KOD FX Neo (Toyobo, Japan) and cloned into pT18mobsacB by in-fusion reaction (Clontech, United States) for the construction of insertion mutants ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized using 1 µg of RNA following manufacturers’ instruction (1st strand cDNA synthesis kit, Takara; 6110A). RT-PCR was performed using SapphireAmp® Fast PCR Master Mix (Takara ...
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bioRxiv - Developmental Biology 2023Quote: ... the TaKaRa DNA Ligation Kit LONG (TaKaRa Bio) was used along with NEB Stable Competent E ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged recombinant proteins were isolated by 1 h incubation with Talon metal affinity resin (Clontech) at room temperature ...