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Citations for Takara Bio :
4501 - 4550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... pEGFP-C1 was purchased from Clontech. pcDNA3.1-Flag-N was obtained from IGBMC cloning facility ...
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bioRxiv - Microbiology 2023Quote: ... with TB Green Advantage qPCR premix (Takara Bio) and the following primers (5’ to 3’) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by blocking solution containing primary antibodies against mCherry (mouse monoclonal 1:500, Takara Bio) and c-Fos (rabbit polyclonal 1:500 ...
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bioRxiv - Systems Biology 2023Quote: ... The cells containing the Cas12-pRG232 insertion were selected with 0.5ug/mL puromycin (Takara, 631305) for three days ...
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using a One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2023Quote: ... 0.8 U/µl recombinant RNase inhibitor (Takara, Cat# 2313B)] and incubated at room temperature for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... The CPER products (25 µl) were transfected into VeroE6/TMPRSS2 cells with TransIT-X2 (Takara, Cat# MIR6003) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The viral RNA copy number was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). Fluorescent signals were acquired using a QuantStudio 1 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... and mouse GAPDH transcripts as described previously 47 using SYBR green reagent (Takara) with primer pairs 1195/1196 ...
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bioRxiv - Plant Biology 2023Quote: The LexA-based Y2H system (Clontech) was used for protein interaction analysis ...
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bioRxiv - Plant Biology 2023Quote: ... and transferred by LR recombination into pB42AD-GW and pGILDA-GW (Clontech), respectively ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified using Nucleospin Gel and PCR Clean-up kit (Takara), and Sanger sequenced using primer IGHG1-F1 (Supplementary Table 5 ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA was reverse-transcribed using a cDNA synthesis kit (TaKaRa) and one μg of each RNA samples was reverse transcribed ...
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bioRxiv - Plant Biology 2023Quote: Transformation of Saccharomyces cerevisiae strain HF7c was performed as described in the Yeast Protocols Handbook (Clontech), with transformants selected on synthetic dropout (SD ...
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bioRxiv - Plant Biology 2023Quote: ... RNA from 30 nymphs was extracted using the RNAiso Plus kit (TaKaRa) according to the manufacturer’s instructions as one biological replicate ...
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed from cDNA using SYBR Green qPCR mix (TaKaRa, RR601A) following the manufacturer’s instructions using the LightCycler 480 system (Roche Diagnostics) ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from cell pellets using Nucleospin RNA Plus XS kit (Takara), and first strand cDNA synthesis was performed using Superscript IV VILO Master Mix with ezDNAse (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... PrimeSTAR Max Premix (2x) (TaKaRa, Japan) was used for PCR with the degenerate primers listed in Supplemental Table S10 ...
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bioRxiv - Plant Biology 2023Quote: ... an aliquot of total RNA was treated with DNase (Takara, Dalian, China) using the standard protocol described by the manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... using SYBR Premix Ex Taq Ⅱ (TaKaRa, Japan). The 25S rRNA gene obtained from Carthamus tinctorius L ...
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bioRxiv - Plant Biology 2023Quote: ... pGBKT7 and pGADT7 (Takara Bio) by using Gateway LR Clonase II (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: Y2H assays were performed with the MatchMaker GAL4 Two-Hybrid System 3 (Takara Bio). Saccharomyces cerevisiae strain AH109 was co-transformed with different pairs of pGADT7 and pGBKT7 harboring IREH1 or B1-Rafs ...
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bioRxiv - Plant Biology 2023Quote: The interaction between PIF4 and MPK4 was examined using the Matchmaker gold yeast two-hybrid system (Takara Bio). PIF4 and MPK4 cDNA were cloned in the pGADT7 and pGBKT7 vectors ...
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bioRxiv - Immunology 2023Quote: ... and concentrated using Lenti-X Concentrator (Takara Bio) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... flanking the site of alterations (HDE-like motifs) were amplified from the genomic DNA using CloneAmp HiFi PCR Premix (Clontech), A-tailed with DreamTaq DNA polymerase and ligated into pGEM-T Easy vector ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... mtDNA was then amplified by PCR using PrimeSTAR® GXL polymerase (Takara). PCR was performed using two sets of slightly overlapping primers to reduce primer bias ...
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bioRxiv - Microbiology 2023Quote: ... anti-GAPDH antibody (Clontech) at a 1/2500 dilution ...
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bioRxiv - Microbiology 2023Quote: ... STM ΔspeED by RNA isolation was performed using TRIzol (Takara) reagent according to manufactures’ protocol RNA was quantified using Thermo-fischer scientific Nano Drop followed by running on 2% agarose gel for checking the quality ...
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bioRxiv - Microbiology 2023Quote: ... QuickCutTM Hind III (TAKARA, 1615), TwistAmp® Basic Kit (TwistDx ...
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bioRxiv - Microbiology 2023Quote: ... The fragments were joined using In-Fusion (TaKaRa), producing plasmid pTV002 ...
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bioRxiv - Microbiology 2023Quote: ... and the RNA was extracted immediately using a bacterial RNA extraction kit (TaKaRa). The purified RNA extracts were reverse-transcribed to cDNA ...
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bioRxiv - Neuroscience 2023Quote: ... 1.25 x105 cells/cm2 were immediately added to each well with HEK293 maintenance media modified with 10% tetracycline-free FBS (Takara Bio, Cat. No. 631367). After 24 h ...
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bioRxiv - Neuroscience 2023Quote: ... Cousin lab was dropped into an pEGFP-N1 CMV mammalian expression vector (Clontech) by In-fusion cloning ...
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bioRxiv - Neuroscience 2023Quote: ... GP2-293 cell line was purchased from (Takara Bio, Kusatsu, Shiga, Japan). PC12 cells were cultured in RPMI-1640 with 2mM L-glutamine (FUJIFILM Wako Pure Chemical ...
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bioRxiv - Neuroscience 2023Quote: ... GP2-293 cells were cultured in DMEM (FUJIFILM Wako Pure Chemical) supplemented with 10% tetracycline-free FBS (Takara Bio).
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bioRxiv - Neuroscience 2023Quote: ... the brain slices were first blocked in 10% goat serum made in 0.1% Triton X-100/1× PBS and then incubated in anti-GFP antibodies (1/1000, RT, overnight, Mouse anti-GFP, 632381, Takara Bio USA, WI) followed by Alexa-488 secondary antibodies (1/200 ...
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bioRxiv - Neuroscience 2023Quote: ... the RNA concentration was determined via Nanodrop and up to 100 ng was used as an input material for library preparation using the Clontech Ultra Low kit (Clontech).
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bioRxiv - Microbiology 2023Quote: ... PrimeSTAR GXL Premix (Takara) and primers listed in supplementary Table 1 ...
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bioRxiv - Microbiology 2023Quote: ... clarified by Lenti-X (TaKaRa). Particle infectivity was measured as previously described (28) ...
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bioRxiv - Microbiology 2023Quote: ... using the In-Fusion HD Cloning system (Clontech) and transformed into One Shot® BL21 Star™ (DE3 ...
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bioRxiv - Microbiology 2023Quote: ... Y2H Gold (pGADT7-PfGEXP15 RVxF) and Y187 (pGBKT7 constructs) yeast strains (Clontech) were mated on SD-LW media ...
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bioRxiv - Molecular Biology 2023Quote: ... tabaci adults was used for the synthesis of first strand cDNA utilizing the make your own mate & plate library system with CDS III adaptor primer and smart III oligo (template switching) as per manufacturer’s instructions (Takara Bio, San Jose, CA, USA). The first strand cDNA was used as template for long distance PCR with 5’ and 3’ primers ...
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bioRxiv - Microbiology 2023Quote: ... Stellar™ Competent Cells (Takara Cat#: 636763) were used to generate clones of the vector containing RBD genes of interest ...
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bioRxiv - Microbiology 2023Quote: ... The culture was then split in half and one tube received 40ul 2ug/ul anhydrotetracycline (Takara, 631310) to induce GFP expression ...
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bioRxiv - Neuroscience 2023Quote: ... the SV40pA was replaced by the 3’UTR of msSOD2 (Kaltimbacher et al., 2006) amplified from mouse brain cDNA (Clontech 637301) using primers AA35 and AA36 and inserted into the XbaI and NotI sites ...
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bioRxiv - Immunology 2023Quote: ... cDNA libraries were constructed using the SMARTer Stranded Total RNA-Seq Kit (v2) – Pico Input Mammalian (Clontech) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Clarified supernatant was incubated with His60 NTA resin (TAKARA Bio) for 1 hour then loaded onto gravity flow columns and eluted with Buffer A containing 200 mM Imidazole ...
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bioRxiv - Genetics 2023Quote: ... Human KISS1 was cloned into pLVX-neo vector (Clontech) for overexpression ...
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bioRxiv - Genetics 2023Quote: ... Viruses were concentrated by using the Lenti X Concentrator (Clontech, 631231), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and plated on 0.2% gelatin coated T75 flasks at a density of 6,666 cells/cm2 in RHBA medium (Takara Bio, Y40001). Media was changed every 2 days ...