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Citations for Takara Bio :
4151 - 4200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PK-15 cells were transfected with PX459-Ifnar1-k/o or PX459-Stat2-k/o plasmids using the TransIT-X2 Dynamic Delivery System (TaKaRa, Cat# V6100) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... We next cloned the synthesized DNA into the pDON-5 Neo-vector (TaKaRa, Kusatsu, Japan, Cat# 3657), which was pre-linearized with NotI-HF (New England Biolabs [NEB] ...
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bioRxiv - Microbiology 2023Quote: ... cloned into a pCold-II vector (Takara Bio Co Ltd., Japan) containing an N-terminal His tag ...
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bioRxiv - Immunology 2023Quote: ... followed by RNA reverse transcription by SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech). After enzymatic fragmentation of cDNA samples by KAPA Frag kit (KAPA Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... supernatants were harvested and concentrated with Lenti-X™ Concentrator (Takara# 631232). Virus pellet was resuspended in 1∼4 mL RPMI1640 medium (with 10% FBS) ...
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bioRxiv - Immunology 2023Quote: ... The 6-His tagged recombinant proteins were purified from the supernatant by gravity-fed through TALON® Metal Affinity Resin (Takara Bio, Shiga, Japan). Following a wash step with PBS (pH 8) ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was performed using PrimeScript reverse transcriptase (Takara) and gene expression was analyzed using the TP SYBR 2x mastermix (TopBio ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed using the Thermal Cycler Dice Real Time System (Takara Bio, Kusatsu, Japan). Primers and templates were mixed with TB Green Premix Ex Taq II (Takara Bio) ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Clontech) and 2 mM L-glutamine (PAN Biotech ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was incubated with 4 ml TALON metal affinity resin (Clontech) at 4°C for 1 hr ...
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bioRxiv - Microbiology 2023Quote: ... LDH release was measured using an LDH Cytotoxicity Detection Kit (Clontech) according to the manufacturer’s instructions and normalized to mock-infected cells.
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bioRxiv - Microbiology 2023Quote: ... All the primers were designed using an online tool provided by TaKaRa (https://www.takarabio.com/learning-centers/cloning/primer-design-and-other-tools ...
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bioRxiv - Microbiology 2023Quote: ... FluPol Zhejiang-H7N9 was then purified using His60 NiNTA Superflow resin (Takara Bio) from the soluble fraction ...
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bioRxiv - Microbiology 2023Quote: ... we performed mutagenesis by overlapping PCR using PrimeSTAR GXL DNA Polymerase (TaKaRa, Cat# R050A) together with the primers IFNAR-F (5′-CGTGGGCCCGCGGCCGCGCCACCATG-3’ ...
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bioRxiv - Microbiology 2023Quote: ... and pMD2.G plasmids using TransIT-293 Transfection Reagent (TaKaRa, Cat# V2700) in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... pCMV-HA-N2 (Takara Bio Inc.,), pET28a(+ ...
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bioRxiv - Microbiology 2023Quote: ... and double amino acid mutations were introduced using PrimerSTAR® Max DNA Polymerase (# R046A, Takara Bio Inc.). The primers used for the mutation generation were listed in SI data.
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bioRxiv - Microbiology 2023Quote: ... The p24 amount in the medium was quantified by Lenti-X p24 Rapid Titer Kit (632200, Takara Bio Inc.) with the p24 control samples ...
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bioRxiv - Microbiology 2023Quote: The cDNA encoding human ZCCHC3 (NM_033089.7) was amplified from cDNA pool of HeLa cells by PCR and cloned into pEGFP-C3 (Takara Bio Inc., Shiga, Japan), pCMV-HA-N2 (Takara Bio Inc.,) ...
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bioRxiv - Microbiology 2023Quote: ... USA) or non-targeting control siRNA with TransIT-X2 Dynamic Delivery System (V6100, Takara Bio Inc.) in Opti-MEM ...
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bioRxiv - Microbiology 2023Quote: ... The cells were cultured again overnight and subjected to the quantification of mRNA by qRT-PCR using the CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (3732, Takara Bio Inc.), One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time ...
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bioRxiv - Microbiology 2023Quote: ... and small intestinal organoids by Total RNA extraction reagent RNAiso Plus (TaKaRa Bio, Shiga, Japan) or NucleoSpin®RNA (MACHEREY-NAGEL ...
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bioRxiv - Molecular Biology 2023Quote: ... and quantitative PCR was performed using SYBR® Premix Ex Taq (Tli RNaseH Plus) (Takara) on a LightCycler 480 II (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The resulting PCR fragment was subcloned into the KpnI-NotI site of the pCAGGS vector20 using In-Fusion HD Cloning Kit (Takara, Cat# Z9650N). Nucleotide sequences were determined by DNA sequencing services (Eurofins) ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial DNA was isolated using a NucleoSpin DNA stool kit (Takara Bio, Shiga, Japan). The V3-V4 regions of the 16S rRNA gene were amplified in each sample ...
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bioRxiv - Microbiology 2023Quote: The Lenti-X 293T cell line (Takara, Cat# 632180) and HOS-ACE2/TMPRSS2 cells (kindly provided by Dr ...
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bioRxiv - Molecular Biology 2023Quote: ... and lentivirus was concentrated with lenti-X concentrator (Takara #631232) to 1/100th of the initial media volume and the lentivirus was resuspended in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... The Prime Script RT Master Mix (Takara Bio, RR036A) was used to synthesize cDNA according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... with TB Green Premix Ex Taq Ⅱ (Takara Bio, RR820B). Primers are in the supplemental materials ...
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bioRxiv - Genomics 2023Quote: ... supplemented with 10% FBS (Takara), 1% NEAA (Gibco) ...
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bioRxiv - Genomics 2023Quote: ... and doxycycline (200 ng/ml; Clontech) on laminin-521 (Biolamina ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... RNA was reverse transcribed using anchored oligo-dT and SMART Scribe RT enzyme (Clontech) allowing to add adapter sequences to the 5’ and 3’ ends of RNA fragments ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 × 106 PBMCs were plated on retronectin coated 6-well plates (Takara Clonetech) with retroviral vectors and centrifuged at 1,000 × g for 40 min ...
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bioRxiv - Neuroscience 2023Quote: ... and eGFP (Clontech. CA, USA) in tsA201 cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated in primary antibody (chicken anti-GFP, Abcam #13970, 1:2000, rabbit anti-DsRed, Takara Bio Clontech #632496, 1:200) for 24 – 48 hours in the dark while shaking at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: RNA extracion from the cells and mouse hearts was performed using the sophisticated Trizol reagent (Takara, Kyoto, Japan). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA was subjected to reverse transcription to generate RNA cDNA using the widely recognized PrimeScriptRT kit (Takara, Kyoto, Japan) provided by the esteemed Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... using PCR and cloned in the pEGFP-N1 vector (Clontech) at the KpnI and NotI restriction sites ...
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bioRxiv - Molecular Biology 2023Quote: ... Accurate quantification of mRNA levels was meticulously conducted by employing the GAPDH reference gene and utilizing the cutting-edge SYBR Green kit (Takara, Kyoto, Japan) on the state-of-the-art Real-time PCR Detection System manufactured by Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplified fragments and vector pBFv-UAS3 were fused using the In-Fusion® HD Cloning Kit (TaKaRa). The oligonucleotide sequences used are shown in Supplementary Table 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... EGFP-SEC61B was generated by cloning the corresponding cDNA into the pEGFP.C1 vector (Clontech, 6084-1). The plasmid pRK5-HA-UB was described previously [56] ...
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bioRxiv - Neuroscience 2023Quote: ... Both back bones and inserts were then agarose-gel purified using NucleoSpin® Gel and PCR Clean-Up columns (Takara Bio USA, Inc.). Purified fragments then mixed at prescribed molar ratio together with In-Fusion HD Cloning Kit ...
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bioRxiv - Neuroscience 2023Quote: ... We used a seamless cloning strategy (In-Fusion, Takara Bio USA, Inc.). Primers were synthesized from the company IDT ...
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bioRxiv - Neuroscience 2023Quote: ... high-fidelity polymerase (Cat#R050A, Takara Bio USA, Inc.) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR reaction mixture for all reactions consisted of 5x PrimeSTAR Buffer (Takara), 200uM dNTPs (Takara) ...
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bioRxiv - Neuroscience 2023Quote: ... 50x PrimeSTAR GXL DNA polymerase (Takara), water up to recommended volume ...
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bioRxiv - Neuroscience 2023Quote: ... Lentivirus was then concentrated using Lenti-X Concentrator (Takara Bio), reconstituted in PBS ...
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bioRxiv - Neuroscience 2023Quote: PCR was performed using 1.25 U PrimeSTAR GXL DNA Polymerase (Takara, Japan) with 0.2 µM of forward and reverse primers ...
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bioRxiv - Immunology 2023Quote: ... 1.25 units of ExTaq DNA polymerase (TAKARA, Shiga, Japan), 5 mM KCl ...
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bioRxiv - Immunology 2023Quote: ... to alanine (A) (L234A, L235A [LALA]) by site-directed mutagenesis with the In-Fusion Cloning System (In-Fusion® HD Cloning Plus; Clontech Laboratories Inc., Shiga, Japan) based on the manufacturer’s protocol [13].