Labshake search
Citations for Takara Bio :
4301 - 4350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... the gateway cassette of the plasmid pmCherry-ccdB was generated by subcloning of ccdB into pmCherry-C1 plasmid (Clontech, Mountain View, CA) using SmaI ...
-
bioRxiv - Biochemistry 2023Quote: ... DNA samples were digested with SacI and S1 nuclease (Takara Bio, #2410) and analyzed by agarose gel electrophoresis followed by staining with SYBR Gold nucleic acid gel stain (Thermo Fisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio, Shiga, Japan); PrimeScript RT-PCR Kit (Takara Bio ...
-
bioRxiv - Developmental Biology 2023Quote: ... cDNA synthesis was performed with SMART-Seq v4 Ultra Low Input RNA Kit (Clontech cat. 634888) according to the manufacturer’s instruction ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were routinely tested for mycoplasma contamination by PCR (6601, Takara Bio). To convert primed hESCs to RSeT or PXGL culture conditions ...
-
bioRxiv - Cancer Biology 2023Quote: ... cDNA was generated with PrimeScript RT Master Mix (Takara). qPCR was performed on StepOnePlus Real-Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... These three segments were ligated sequentially by overlap PCR and inserted into XhoI/NotI linearized Phr-SIN vector by In-fusion cloning (Clontech) to produce full length CD1d SCD ...
-
bioRxiv - Neuroscience 2023Quote: ... using In-Fusion cloning system (#639648, Takara Bio). All constructs were verified by direct sequencing ...
-
bioRxiv - Neuroscience 2023Quote: EGFP-tagged rat PKCδ cDNA were cloned into EcoRI and BamHI digested pRetroX-TetOne-Puro (#634307, Takara Bio) using In-Fusion cloning system (#639648 ...
-
bioRxiv - Immunology 2023Quote: ... TSC were transduced by spinoculation on retronectin-coated plate (Takara) with 1:1 mixture of culture medium and retroviral supernatant ...
-
bioRxiv - Microbiology 2023Quote: ... 2×SYBR Green PCR Mastermix (RR820A) and reverse transcription kit (RR047A) were purchased from TAKARA company ...
-
bioRxiv - Microbiology 2023Quote: ... to ligate the variant insert pool into BsmBI-digested pHW2000 plasmid and transformed Stellar™ Competent Cells (Takara, #636763) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... mouse anti-mCherry monoclonal antibody (1:500; Takara Bio Cat# 632543), Alexa Fluor 488-conjugated anti-chicken IgG antibody (1:500 ...
-
bioRxiv - Microbiology 2023Quote: ... TwistAmp® Basic Kit (TwistDx, TABAS03KIT), Milenia HybriDtect1 (TwistDX, Milenia01), UltraPure DNase/RNase-Free Distilled Water (Thermo, 10977015) and RNase inhibitor (Takara, 2313A). Thermo Fisher Nanodrop 1000 Spectrophotom was used to quantify nucleic acid ...
-
bioRxiv - Cancer Biology 2023Quote: ... forward-Trail and reverse-Trail were generated in PZH vector amplified and extracted using In-Fusion HD Cloning kit (Clontech). Plasmids were sent for Sanger sequencing with reverse and forward primer to control any mutation point ...
-
bioRxiv - Microbiology 2023Quote: ... TaKaRa MutanBEST Kit (Takara, R401), QuickCutTM Hind III (TAKARA ...
-
bioRxiv - Microbiology 2023Quote: ... Then similar protocol was used to isolate total RNA using TRIzol (Takara) reagent according to manufactures’ protocol.
-
bioRxiv - Genetics 2023Quote: ... The PCR fragments were purified and inserted into the vector pigAct88F-GFP [58] through recombinational cloning (In-Fusion, Takara). Primers used for the PCR reactions (sequences for recombination are underlined):
-
bioRxiv - Evolutionary Biology 2023Quote: ... We amplified a 1.4 kb genomic fragment with PCR primers AIP3F (5’- GGCGCTATACCCGCTCGTGTCC-3’) and AIP5R2 (5’-CTTCATATTTGAAGACGAGGGAGG-3’) using 10 ng genomic DNA and Advantage DNA polymerase (Clontech) with the following cycling conditions ...
-
bioRxiv - Evolutionary Biology 2023Quote: The PCR products (3.2 kb or 2.0 kb of HBV-DNA if the 3.2-kb DNA was unavailable) were cloned into the vector PMD-19T with T4 DNA Ligase (Takara, Japan) and transformed into TOP10 Escherichia coli competent cells (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: Total RNA of each strain was extracted with the RNA extraction kit (Takara biomedical technology, Beijing, China) following the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2023Quote: ... transcripts of the target genes were amplified with the corresponding primer pairs (S2 Table) and quantified with SuperReal PreMix Plus (SYBR Green) (Takara biomedical technology, Beijing, China), using the 18S rRNA was used as control ...
-
bioRxiv - Microbiology 2023Quote: ... and genomic DNA was removed using DNase I (TaKaRa). The transcriptome libraries were constructed by using the TruSeqTM RNA sample preparation Kit from Illumina (San Diego ...
-
bioRxiv - Molecular Biology 2023Quote: ... which was supplemented with 10% Tet System Approved FBS (TaKaRa), penicillin/streptomycin ...
-
bioRxiv - Molecular Biology 2023Quote: ... was cloned in a home-made inducible vector derived from pLVX-Tight-Puro (Clontech) to make it SIN by a deletion in the 3’ LTR ...
-
bioRxiv - Molecular Biology 2023Quote: ... An RNA amount equivalent to 12 ng was used to prepare the sequencing library using SMARTer® smRNA-Seq Kit for Illumina® (Takara 635031) according to the kit manual till cDNA synthesis ...
-
bioRxiv - Neuroscience 2023Quote: ... rabbit anti-dsred (Clontech, 632496;1:10,000; RRID:AB_10013483), choline acetyltransferase (goat anti-ChAT ...
-
bioRxiv - Molecular Biology 2023Quote: ... media was removed and concentrated using lenti-X concentrator solution (Takara Bio #631232). Concentrated viral particles were distributed into single-use aliquots and stored at −80°C until use ...
-
bioRxiv - Genetics 2023Quote: ... sgRNA was produced using sgRNA IVT kit (Takara Bio) by following the manufacturer’s instructions and RNA was isolated by Trizol (Invitrogen) ...
-
bioRxiv - Genomics 2023Quote: ... coli cells (Takara Bio, catalog #636763), colonies were picked ...
-
bioRxiv - Genomics 2023Quote: ... SMART-Seq v4 kit (Clontech, 634893) was used for the reverse transcription and cDNA amplification according to the manufacturer’s specifications ...
-
bioRxiv - Molecular Biology 2023Quote: ... The resultant PCR products were inserted into a pmCherry-C1 (Clontech) using XhoI/BamHI restriction enzyme recognition sites ...
-
bioRxiv - Molecular Biology 2023Quote: ... and HEK293-FT cells were used to prepare lentiviral particles according to the instructions in the Lenti-XTM Lentiviral Expression System Manual (Clontech). Doxycycline-inducible ZDHHC20 expressing HEK293T cells were prepared by transduction of 2.5 X 105 low-passage cells with lentivirus ...
-
bioRxiv - Molecular Biology 2023Quote: ... C-FLAG-D20 was subcloned into the pLVX-TetOne-Puro (Clontech) and in-house attb vectors ...
-
bioRxiv - Genomics 2023Quote: ... 800 μL of dNTPs (Takara), 50 μL of P5 stagger primer mix (stock at 100 μM concentration) ...
-
bioRxiv - Genomics 2023Quote: ... a master mix consisted of 150 μL DNA Polymerase (Titanium Taq; Takara), 1 mL of 10x buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... site-directed mutagenesis was performed using a PrimeSTAR mutagenesis basal kit (Takara Bio) with the following primers ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2 × 106 HEK293T cells were transfected with 7 μg of Env expressor and 1 μg of a green fluorescent protein (GFP) expressor (pIRES2-EGFP; Clontech) with the calcium phosphate method ...
-
bioRxiv - Molecular Biology 2023Quote: ... site-directed mutagenesis was performed by using a PrimeSTAR mutagenesis basal kit (Takara Bio) and pEGFP-C1-mADAR1 p150 as a template with the following primers ...
-
bioRxiv - Genomics 2023Quote: ... 4 µl of Dr.GenTLE precipitation carrier (Takara, cat. no. 9094) and 4 µl sodium acetate ...
-
bioRxiv - Genomics 2023Quote: ... 2.5 U Takara Epi Taq HS (Takara, cat. no. R110A, 5 U/µl), 2.5 mM MgCl2 ...
-
bioRxiv - Genomics 2023Quote: ... Lentivirus containing the overexpression plasmid was generated using LentiX Packaging Single Shots (Clontech, 631278) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... All the mutant constructs of CE were prepared from pcDNA4/TO-bio-myc-mCE by using In-Fusion Site Directed Mutagenesis kit (Takara Bio). mCherry-H2B ...
-
bioRxiv - Molecular Biology 2023Quote: ... The Matchmaker two-hybrid system 3 (Clontech Laboratories, Mountain View, CA, USA) was used for the yeast two-hybrid assay ...
-
bioRxiv - Molecular Biology 2023Quote: ... were constructed by cloning the full-length coding region into the pGADT7 vector using the In-Fusion HD Cloning Kit (Clontech Laboratories, Mountain View, CA, USA). The Matchmaker two-hybrid system 3 (Clontech Laboratories ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cytotoxicity was assessed in parallel using the LDH Cytotoxicity Detection Kit (Takara Bio Inc.)
-
bioRxiv - Genomics 2023Quote: ... The obtained PCR products were purified using NucleoSpin Gel and PCR Cleanup kit (Takara Bio, Shiga, Japan) and sequenced directly or were cloned into the pTAC-1 vector (Biodynamics ...
-
bioRxiv - Genomics 2023Quote: ... The RT-PCR products were treated with NucleoSpin Gel and PCR Cleanup kit (Takara Bio) and directly analyzed using Sanger sequencing (Eurofins Genomics) ...
-
bioRxiv - Genomics 2023Quote: ... 5 µm-thick sections were cut and immunostained with anti-GFP antibody (living colors, Clontech, 632592) and secondary anti-rabbit IgG with Alexa fluor 488 (Thermo Fisher) ...
-
bioRxiv - Immunology 2023Quote: ... the non-tissue culture 24-well plates were coated with 7 μg/ml RetroNectin (TAKARA) for 3 hours at 37°C ...