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Citations for Takara Bio :
4551 - 4600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... mCherry and Venus ORFs were inserted into the MCSs of pBI-CMV1 (631630, Clontech, TakaraBio). WT ...
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bioRxiv - Cell Biology 2023Quote: ... Jerusalem) (73) was subcloned into the XhoI-BamHI sites of the pEGFP-C1 vector (Clontech, Mountain View, CA), and the corresponding SB203580-resistant mutant (T106M ...
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bioRxiv - Cancer Biology 2023Quote: ... The construct containing Y181G-coding point mutation was generated by inverse PCR of Zdhhc20WT plasmid using CloneAmp HiFi PCR Premix (Takara) and joining the ends of the PCR product using InFusion cloning.
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bioRxiv - Cell Biology 2023Quote: ... the respective sequence was amplified by PCR from pEGFP C1 (Clontech, Takara Bio Europe ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then induced for the indicated amount of time with 1 μM Shield-1 ligand (Takara bio) to stabilize the TRF1-FokI protein and 1 μM 4-OHT (Cayman Chemical ...
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bioRxiv - Cell Biology 2023Quote: ... the respective sequence was amplified by PCR from pEGFP C1 (Clontech, Takara Bio Europe ...
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bioRxiv - Cell Biology 2023Quote: ... pAxCAwtit (Takara), which contains the CAG promoter and the entire genome of type 5 adenovirus ...
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bioRxiv - Cell Biology 2023Quote: ... E-cadherin antibody (HECD-1, 1:1000 IF, 1:1000 WB) was from Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: ... with 0.1% Westase (Takara Bio, Kusatsu, Japan) in McIlvain buffer (37 mM citrate ...
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bioRxiv - Cell Biology 2023Quote: ... All plasmid ligation step was done using In-fusion seamless cloning (Takara Bio). Appropriate mutagenesis was done using QuikChange Lightning Multi Site-Directed mutagenesis kit (Agilent) ...
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bioRxiv - Cell Biology 2023Quote: ... and reverse transcription was performed using RNA to cDNA EcoDry™ Premix (Clontech – Takara Bio) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... using the In-Fusion HD cloning kit (Clontech), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and CDCA8 cDNA was amplified via PCR from the HeLa cDNA library and ligated into the pQCXIP vector (Takara Bio, Shiga, Japan) with N-terminal GFP ...
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bioRxiv - Cell Biology 2023Quote: ... and concentrated 10-fold using Lenti-X concentrator (Takara). Lentivirus aliquots were stored at -80 °C until functional titration and use.
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from ONB tumor tissue samples using RNAiso Plus reagent (Takara Bio, Inc.) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... RNAseH was obtained from Takara Clontech (# 2150A) ...
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bioRxiv - Cancer Biology 2023Quote: ... Adeno-X Maxi Purification Kit and Adeno-X Rapid Titer Kit from Clontech were used ...
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bioRxiv - Cell Biology 2023Quote: ... 30 cycles of 98 °C for 10 sec followed by 68 °C for 1 min using PrimeSTAR HS DNA Polymerase with GC buffer (Takara, R044A). The primers used were as follows ...
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bioRxiv - Cell Biology 2023Quote: ... and inserted into the pScarlessHD-C-3xVHH05-6XMS2-DsRed vector via InFusion cloning (Takara Bio). The DNA plasmid of pScarlessHD-5’ msps homology arm-C-3xVHH05-6XMS2-DsRed-3’ msps homology arm was co-injected with pCFD5-gRNA#1 and pCFD5-gRNA#2 by BestGene ...
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bioRxiv - Cell Biology 2023Quote: ... crossed with WT BDF1 males at E0.5 and electroporated with RNP complex of Mavs targeting sgRNA (100 ng μL-1) and Cas9 protein (Takara Bio; 500 ng μL-1) using NEPA21 electroporator ...
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bioRxiv - Biophysics 2023Quote: ... and precipitated with Lenti-X concentrator (Clontech) at 4 °C for 12 h ...
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bioRxiv - Cancer Biology 2023Quote: ... The SMARTer Ultra Low Input RNA Kit for Sequencing v4 (Clontech Laboratories) was used to generate first strand cDNA from 0.1 to 1.0 ng total-RNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Ecotropic lentivirus was produced using Lenti-X 293T cells (Takara). 743 and 732 cells were transfected with pLV-EF1a -LCP1-HA-IRES-Puro construct together with psPAX2 and pMD2.G using GeneJuice (Millipore) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ecotropic lentivirus was produced using Lenti-X 293T cells (Takara). These cells were transfected with lentiCRISPRv2 vector expressing sgRNA together with psPAX2 and pMD2.G using GeneJuice (Millipore) ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus particles were collected 24 hours and 48 hours after transfection and concentrated with Lenti-X™ Concentrator (Takara, California, USA, Cat#631232). The pellets were then resuspended with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription and DNA amplification was performed with SmartSeqV4 (Takara) using 10 ng of RNA as template ...
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bioRxiv - Cancer Biology 2023Quote: Coding regions of the heavy-chain variable domains were amplified with two PAGE-purified primers (CALL001: 5’-GTCCTGGCTGCTCTTCTACAAGG-3’ and CALL002: 5’-GGTACGTGCTGTTGAACTGTTCC-3’) using LA Taq polymerase (TAKARA Bio Inc., Shiga, Japan). The amplified fragments were separated on a 1.5% low-melting-temperature agarose gel (Lonza Group AG ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA libraries were constructed using the immunoprecipitated mRNA and SMARTer PCR cDNA Synthesis Kit (Clontech, Cat: 634926), and sequenced on a HiSeq 2500 system (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: HEK 293T cell line (Lenti-X™ 293T, Takara Bio, San Jose, CA, USA) was cultured in DMEM High Glucose (DMEM-HG cat# SH30243.02 ...
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bioRxiv - Cell Biology 2023Quote: ... and then inserted into the pEGFP-N1 vector (Clontech) for the expression of ITGβ5-GFP ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragments encoding human zyxin were amplified from the cDNA of U2OS cells and then inserted into the pmCherry-N1 vector (Clontech) for the expression of zyxin-RFP.
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bioRxiv - Cell Biology 2023Quote: ... The DNA fragment of RFP-CaaX was inserted into the pEGFP-C1 vector (Clontech) to replace the EGFP for the mammal expression ...
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bioRxiv - Cell Biology 2023Quote: ... by InFusion Snap assembly (Takara). This plasmid was linearized with Sbf I and Xho I and a synthetic g-block (IDT ...
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bioRxiv - Developmental Biology 2023Quote: ... Full length cDNA was generated using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe, 634891) according to manufacturer’s instructions with 15 cycles of PCR for coding DMA (cDNA ...
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bioRxiv - Immunology 2023Quote: The medium from transfected HEK293T cells was replaced with Opti-MEM containing 1 μM of B/B Homodimeriser (AP20187; Clontech) and incubated for 30 min ...
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bioRxiv - Genomics 2023Quote: ... Illumina sequencing libraries were prepared from ChIP-enriched DNA using ThruPLEX DNA-seq library preparation kit (Takara Bio) with up to 10ng of input DNA and 8-15 PCR cycles ...
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bioRxiv - Immunology 2023Quote: 150-200 tuft cells were sorted directly into lysis buffer from the SMART-Seq v4 Ultra Low Input RNA Kit (Takara) and cDNA generated following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Wt or mutated human (h)ALPK1 cDNA constructs were cloned into the pCMV-myc plasmid (Takara Bio Inc) and were all siRNA resistant against the hALPK1 siRNA (s37074 ...
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bioRxiv - Immunology 2023Quote: ... and 2 μl SMART MMLV reverse transcriptase (Clontech, Takara Bio). The mixture was incubated under the following conditions ...
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bioRxiv - Immunology 2023Quote: ... The mixture was then chilled on ice for 2 min and then mixed with 4 μl 5x first-strand buffer (Takara Bio), 2 μl 100 mM DTT ...
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bioRxiv - Immunology 2023Quote: ... and 2 μl SMART MMLV reverse transcriptase (Clontech, Takara Bio). The mixture was incubated under the following conditions ...
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bioRxiv - Immunology 2023Quote: ... SMART MMLV reverse transcriptase (639524) and carrier DNA (630440) were purchased from Clontech, Takara Bio ...
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bioRxiv - Immunology 2023Quote: ... Random primer (Hexadeoxyribonucleotide mixture, pd(N)6) (3801) was purchased from Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: We used an hESC line that transiently expresses the C-terminal region (catalytic domain) of histone demethylase JMJD3 in a doxycycline (DOX, Takara Bio Inc, Japan) inducible manner (12 ...
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bioRxiv - Genetics 2023Quote: Total RNA extracted from freshly harvested spikes at single ridge end-stage with the RNAprep pure Plant Kit (Biofit Biotechnologies co. Ltd, Chengdu, China) was digested with RNase-free DNase (Takara) to remove residual genomic DNA ...
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bioRxiv - Genetics 2023Quote: ... The RNA was reverse-transcribed into cDNA by using a Prime ScriptTM RT Reagent Kit (TaKaRa, Kyoto, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... cDNA was generated from 2 ng of RNA using the Smart-Sq V4 Ultra Low Input RNA Kit (Takara, 634894). Next ...
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bioRxiv - Genetics 2023Quote: ... The whole fkbA locus from the FK506 resistant isolates was amplified using primers JOHE52223 and JOHE52224 and LA Taq DNA polymerase for long-range PCR with GC Buffer I (Takara). PCR conditions were optimized for long fragments as follows ...
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bioRxiv - Genetics 2023Quote: ... using infusion cloning (Takara).
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bioRxiv - Genetics 2023Quote: ... The 3’ MYH7-eGFP and SV40pA fragments were isolated from a full-length MYH7 cDNA cloned in-frame into pEGFP-N1 (Clontech Laboratories) by PCR with a SalI site at 3’-end ...