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Citations for Takara Bio :
4601 - 4650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... plasmids were co-transfected into Lenti-XTM 293T cells (Clontech Laboratories, 632180) using Lipofectamine 3000 (Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... MT-4 cells (gift from(Nakashima et al., 1990) were transfected with a plasmid containing LTR-driven EGFP expression plasmid (Clontech Laboratories Inc, USA) and pFX2 (Invitrogen Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... by inserting mCherry amplified from mCherry-C1 (Clontech) using 5’-CGGCATTAATGATCTGGCCTCCGCGCCGGGT-3’ and 5’-GGCCGCTAGCCGACCTGCAGCCCAAGCTTCGTC-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was amplified using Emerald Amp GT PCR mix (Takara Bio, Japan) on Verti 96 well thermocycler (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The recombinant proteins were purified using a TALON Metal (Cobalt) Affinity Resin column (Clontech Laboratories, Inc., Palo Alto, CA, USA) and eluted with a linear gradient of imidazole (0–1,000 mM ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Constructs were midi-prepped (Takara Bio), and for site-directed transgenesis ...
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bioRxiv - Genetics 2023Quote: ... France] using the SMART-Seq v4 UltraLow Input RNA kit (Clontech) followed by the Nextera XT DNA sample preparation Kit (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... Primer specificity and amplification efficiency for genes of interest were verified by performing reactions on a dilution series of human reference cDNA (Takara, cat# 636693). Gene expression relative to GAPDH and RPLP0 house-keeping genes was calculated in Microsoft Excel ...
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bioRxiv - Developmental Biology 2023Quote: ... The transcripts were quantified by using SYBR Green Master Mix: SYBR green based TB Green Premium Ex Taq II (Takara, RR82WR) in the PCR instrument Light Cycler 480 (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... TRE3Gs and Tet-ON 3G were amplified by PCR from AAVpro Tet-One Luc Control Vector (Clontech, Cat# 634311), Kaede from in-house recombineering cassette (Zheng et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Addgene plasmid #17398) digested with EcoRI using In-Fusion HD system (Clontech). The fragments of interest were PCR amplified using high-fidelity Pfu DNA polymerase (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Linear DNA fragments were amplified via PCR using CloneAmp HiFi PCR premix (Takara Bio, 639298). PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... coli (Takara Bio, 636763). DNA was extracted by miniprep (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were cut from agarose gels and purified using the Nucleospin gel purification kit (Takara Bio, 240609). Final plasmids were constructed using In-Fusion HD (Takara Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-DsRed 1:100 (Takara Bio Clontech 632496), mouse anti-mCherry 1:100 (Takara Bio Clontech 632543) ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-mCherry 1:100 (Takara Bio Clontech 632543), mouse anti-GFP 1:250 (Abcam Ab1218) ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-DsRed 1:100 (Takara Bio Clontech 632496), mouse anti-mCherry 1:100 (Takara Bio Clontech 632543) ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-mCherry 1:100 (Takara Bio Clontech 632543), mouse anti-GFP 1:250 (Abcam Ab1218) ...
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bioRxiv - Developmental Biology 2023Quote: ... Each individual somite was placed in 10μL of lysis buffer (Takara) containing RNase inhibitor ...
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bioRxiv - Developmental Biology 2023Quote: ... using the SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (TaKaRa, 634891). RNA-seq libraries were generated from 100pg of amplified cDNA using the NEXTERA XT DNA Library Preparation kit (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... under standard conditions with oligo(dT) or random hexamer primers and Recombinant RNase Inhibitor (RRI, TAKARA). Then the cDNA was subjected to quantitative RT-PCR (qRT-PCR ...
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bioRxiv - Developmental Biology 2023Quote: RNA isolation was performed using the RNAiso Plus (TAKARA) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µg of total RNA was converted to cDNA using M-MLV reverse transcriptase (TAKARA) under standard conditions with oligo(dT ...
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bioRxiv - Immunology 2023Quote: ... and cDNA was prepared using the SMARTer Ultra Low RNA kit (Clontech Laboratories) for Illumina Sequencing following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... cDNA synthesis was performed using MMLV reverse transcriptase (Clontech) with oligo(dT ...
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bioRxiv - Immunology 2023Quote: ... All expression plasmids for transient expression were cloned into the pCS2+ vector backbone and cloned using InFusion HD (Clontech). Constitutive lentiviral expression was performed using pCDH vector constructs (System Biosciences ...
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bioRxiv - Immunology 2023Quote: ... cryopreserved BALF cells in Cellbanker1 (Takara #210409) were thawed in PBS and stained with Cell-ID™ Cisplatin-198Pt (Standard Biotools #201198 ...
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bioRxiv - Immunology 2023Quote: ... 110 ng of RNA were retrotranscribed in a final volume of 10 μL using the PrimeScript RT reagent Kit (Takara, Kusatsu, Japan) with a combination of oligo-d(T ...
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bioRxiv - Immunology 2023Quote: ... and 5U of Recombinant RNAse inhibitors (Takara), and then were washed in 1x PBS ...
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bioRxiv - Immunology 2023Quote: ... Full-length cDNAs were generated directly from ~10,000 cells per sample using oligo(dT) priming and the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), and 150 pg of cDNA was used to prepare strand-specific paired-end sequencing libraries (Nextera XT ...
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bioRxiv - Immunology 2023Quote: ... RetroNectin (Takara, #T100B), and when specified ...
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bioRxiv - Immunology 2023Quote: ... and qPCR was performed using a Roche LightCycler 480 with SYBR green system (Takara). Below are the primers used for this study:
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... and CCR6high and transduced with lentivirus particles at an MOI of 5 using retronectin (TaKaRa) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were harvested and resuspended in retroviral supernatant and centrifuged for 90 minutes at 1000g at 32°C on retronectin (TaKaRa) coated plates.
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bioRxiv - Cell Biology 2023Quote: ... using SYBR Premix Ex Taq II (Takara) in conjunction with primer sets specific to human SOD1 (hSOD1 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were subsequently mixed with 10X Loading Buffer (Takara) and resolved on agarose gels using GelRed Nucleic Acid Gel Stain (Biotium ...
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bioRxiv - Cell Biology 2023Quote: ... using SYBR Premix Ex Taq II (Takara) reaction mix with primer sets specific to guide strand cDNA ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription (RT) reactions were performed with 1 μg total RNA using the PrimeScript RT kit with gDNA Eraser (Takara). The resulting cDNA was amplified in triplicate on the 480 Real-Time PCR system (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... plus any amplicon smaller than the 9246 bp WT band for the large indel screen were gel purified with the NucleoSpin Gel clean up kit (Takara Bio ...
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bioRxiv - Cell Biology 2023Quote: — Lenti-X 293T cells purchased from Clontech Labs cat no ...
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bioRxiv - Cell Biology 2023Quote: ... whilst Lenti-X HEK293T cells were sourced from ClonTech. Both cell lines were cultured in DMEM with 10% FBS and 1% GlutaMAX ...
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bioRxiv - Developmental Biology 2023Quote: ... and RT-PCR was performed to detect sorcs2 mRNA in wt embryos using the Titanium One-Step RT-PCR kit (Clontech) with primers 5’-TTTTGCGCACCTGTACCCAGCTG-3’ and 5’-TAACGCGCTCCTGAAGCAGAGTC-3’ for detection of mRNA after injection of different amounts of sMO and 5’-TTTTGCGCACCTGTACCCAGCTGTGTT-3’ and 5’ TAACGCGCTCCTGAAGCAGAGTCCATT-3’ for the different developmental stages ...
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bioRxiv - Developmental Biology 2023Quote: ... then immunized against DsRed (1/1000, Takara Bio, lot #CDSO0219101) followed by a secondary antibody coupled to AlexaFluor 594 (1/500 ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR condition modifications to amplify RACE products are as followed using SeqAmp DNA Polymerase (Takara Bio): Fat1 (TA ...
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bioRxiv - Developmental Biology 2023Quote: SMARTer RACE 5’/3’ Kit from Takara Bio was used following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and or DsRed (1/1000, Takara Bio, lot #2103116) in a solution containing 0.5% Triton-X100 ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-DsRed (1:300, Clontech, #632496), chicken anti-mCherry (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... and amplified with PrimeSTAR GXL DNA Polymerase (TaKaRa) or with SYBR Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... DsRed (Clontech, cat#632392, 1:500), GFP (Thermo ...
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bioRxiv - Developmental Biology 2023Quote: ... the RNA was immediately used for library preparation using the SMARTer smRNA-Seq Kit (Takara), with the following modifications ...