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Citations for Takara Bio :
4251 - 4300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Specific primers were designed for this purpose and PCR was done using the Advantage 2 Polymerase kit (Clontech). Complementary 60 bp-ends to each target sequence on all Mycoplasmas species genome used in this study ...
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bioRxiv - Synthetic Biology 2023Quote: ... SD-Trp-Ura or SD-His-Leu (Clontech Takara).
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bioRxiv - Synthetic Biology 2023Quote: ... met14) was grown at 30 °C in YPDA medium (Clontech Takara). Yeast transformants were selected in Synthetic Defined (SD ...
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bioRxiv - Synthetic Biology 2023Quote: ... met14) was grown at 30 °C in YPDA medium (Clontech Takara). Yeast transformants were selected in Synthetic Defined (SD ...
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bioRxiv - Systems Biology 2023Quote: ... and 1.5μl Titanium Taq DNA Polymerase (Takara). The PCR conditions were ...
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bioRxiv - Immunology 2023Quote: ... All constructs were cloned in the expression vector pLVX-Tight-Puro (Clontech Laboratories), between 5’ NotI and 3’ EcoRI restriction sites ...
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bioRxiv - Immunology 2023Quote: ... inducible cell lines were generated using the Tet-On Advanced Inducible Expression System (Clontech Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Purified RNA was quantified and used for cDNA preparation using the PrimeScript 1st strand cDNA Synthesis Kit (Takara) with 1 μg of extracted RNA ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated by NucleoSpin® RNA Plant RNA isolation kit (Takara Bio, Kusatsu, Japan) according to the manufacture’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... was amplified from Tak-1 genomic DNA by PCR using KOD plus with the primers in Table S1 and cloned into the unique NotI site of pENTR/D-TOPO-MpARK by In-Fusion system (Takara) to generate pENTR/D-TOPO-MpARKpro:MpARK ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μl of cell extract was used in a buffer containing final concentrations of 52 mM HEPES pH 7.4 (Takara), 35 mM KGlu (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... the expression of GADD34Δ and K3L was induced by adding 1 µg/ml of doxycycline (Takara). After one additional day ...
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bioRxiv - Immunology 2023Quote: ... Plates were coated with 5μg/mL of target peptide using coating reagent from the Takara Peptide Coating Kit (Takara cat. #MK100). Measles peptide was utilized as a negative control ...
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bioRxiv - Cancer Biology 2023Quote: ... Second-generation lentiviral transfection was performed in LX293T cells (Takara) with pCMV-VSVG and pCMV-delta8.2 packaging plasmids ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies against E-cadherin (clone ECCD2 Takara, M108), c-Jun (CST #9165) ...
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bioRxiv - Cancer Biology 2023Quote: GFP-expressing HPAF-II cells were engineered via second-generation lentiviral transfection with LX293T cells (Takara) using pLX302-EGFP plasmid (Kevin Janes ...
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bioRxiv - Microbiology 2023Quote: ... then circularized using In-Fusion SNAP Assembly Master Mix (Takara Inc.), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The RNA was then reverse-transcribed into complementary DNA (cDNA) using the PrimeScript cDNA Synthesis Kit (Takara). The DENV 3 cDNA was subsequently amplified by PCR ...
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bioRxiv - Systems Biology 2023Quote: ... and 1 μl 10X Titanium Taq DNA Polymerase (Takara). The PCR conditions were ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA libraries were prepared with SMART-Seq v4 RNA Ultra Low Input (Takara) + Nextera XT (Illumina) ...
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bioRxiv - Plant Biology 2023Quote: ... Real-time PCR was performed on a thermal cycler Dice Real Time System (Takara) using KOD SYBR qPCR Kit (Toyobo ...
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bioRxiv - Plant Biology 2023Quote: ... and was cloned into the AscI site of the pENTR/D-TOPO entry vector containing full length MpNEK1 CDS (Otani et al., 2018) by In-fusion system (Takara). The resulting vector pENTR/D-TOPO-MpNEK1-mCitrine was subjected to LR reaction to transfer MpNEK1-Citrine fusion into the Gateway binary vector pMpGWB144 (MpEF1α pro:XVE >>LexA operator:Gateway cassette).
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from tissues using the RNAiso Plus kit and treated with DNase I (TaKaRa, China). The purified RNA was used for first-strand cDNA synthesis ...
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bioRxiv - Plant Biology 2023Quote: ... Protein concentration was measured using a BCA assay kit (Takara Bio Inc., Kusatsu, Siga, Japan). Twenty micrograms (µg ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were then lifted using TrypLE and total RNA was extracted using the Takara RNA plus kit following manufacturer’s instructions (Takara). 5 µL of total mRNA was converted to total cDNA using random hexamers following manufacturer’s instructions (Verso ...
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bioRxiv - Immunology 2023Quote: ... 500 ng of RNA was used for cDNA synthesis using a PrimeScript RT Reagent Kit (Takara, RR037A). 2 μL of diluted cDNA (1:50 ...
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bioRxiv - Cell Biology 2023Quote: ... BsrGI digested and ligated into a likewise digested C1 (Clontech) backbone ...
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bioRxiv - Molecular Biology 2023Quote: ... Twenty microliter reactions were analyzed as outlined by the manufacturer (TaKaRa). The relative fold-changes of the transcripts were calculated using the comparative CT(2-ΔΔCT ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 1 μg of total RNA was reverse-transcribed with random primers using the PrimeScript™ 1st strand cDNA Synthesis Kit (TaKaRa) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was conducted using TB Green fluorescence (TaKaRa) on a LightCycler 480 II System (Roche Diagnostics ...
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bioRxiv - Cell Biology 2023Quote: ... cultures were split into two 2-ml cultures of which one was supplemented with 250 nM rapalog (Clontech). Mislocalisation of the target protein was verified by live-cell microscopy.
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bioRxiv - Cell Biology 2023Quote: ... Correct disruption and integration were confirmed by genomic PCR at the 5′ and 3′ ends using KOD FX Neo (TaKaRa Bio Inc., Japan). We confirmed that N-terminally GFP-tagged Bqt4 is functional ...
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bioRxiv - Genetics 2023Quote: ... China and produced via the Lenti-X Single Shot system (Clontech). 72 hours later ...
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bioRxiv - Genetics 2023Quote: ... China and produced via the Lenti-X Single Shot system (Clontech). 72 hours later ...
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bioRxiv - Developmental Biology 2023Quote: ... was eliminated by digesting with genomic DNA eraser buffer and cDNA was obtained by reverse transcription of RNA using PrimeScript RT reagent Kit with gDNA Eraser (Takara, RR047A). To measure the values of genes specifc primers ...
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bioRxiv - Microbiology 2023Quote: ... Subsequently the 1086 bp downstream of the pv6 gene was amplified using primers CVO675 and CVO676 and introduced into pBLD701 at the AflII site using InFusion (Takara), producing pBLD702 ...
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bioRxiv - Microbiology 2023Quote: ... to amplify the 3’ end and CloneAmp (Takara Bio) for amplification of the 5’ end.
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bioRxiv - Microbiology 2023Quote: The FLAG-vPOL construct was generated by amplifying the MHV68 vPOL from the recombinant MHV68 M3-Luc bacterial artificial chromosome (BAC) (39) with PCR followed by insertion in the p3xFLAG-Myc-CMV-24 vector (CloneTech Takara Biotech, Mountainview, CA) at the EcoRI and SalI restriction sites ...
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bioRxiv - Microbiology 2023Quote: ... and ligated to the corresponding sites in pTWV228 (Takara bio). The resulting plasmid was named pRM1071.
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bioRxiv - Immunology 2023Quote: ... qRT-PCR was performed using the SYBR® Premix Ex Taq™ II (Takara, Shiga, Japan) Kit and a StepOnePlus Real-Time PCR instrument ...
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bioRxiv - Immunology 2023Quote: ... pE2-Crimson (Clontech) and pFPV25.1 67 ...
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bioRxiv - Immunology 2023Quote: ... low-passage Lenti-X 293T cells (Clontech) were seeded into a T75 flask and transfected the following day using PEI Max (Polyethylenimine) ...
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bioRxiv - Immunology 2023Quote: ... using the In-Fusion HD cloning kit (Clontech). To make SPPL3 and B3GNT2 double knockout cells ...
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bioRxiv - Developmental Biology 2023Quote: ... and cloned it (in frame) into the linearized LV-GFP plasmid using In-Fusion® Snap Assembly Master Mix (Takara #638947). High titer viruses were generated as previously described 56 ...
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bioRxiv - Developmental Biology 2023Quote: ... using CloneAmp™ HiFi PCR Premix (Takara 639298) and cloned it (in frame ...
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bioRxiv - Biophysics 2023Quote: Retroviral constructs encoding HA3-CXCR4 wild-type or designed variants were generated using the In-Fusion HD Cloning Kit (Takara, ref: 638933). Sequences of interest from the expression constructs mentioned above were amplified by high-fidelity PCR (CloneAmp HiFi PCR Premix ...
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bioRxiv - Cell Biology 2023Quote: Lentivirus were generated from transiently transfected LentiX-HEK293T cells (Takara, catalog #: 632180) and collected after 60 hours from the media ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.4) and the protein contents of the cell lysate were quantified using a BCA Protein Assay Kit (TAKARA, T9300A) following the manufacturer’s protocol (Song et al. ...
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bioRxiv - Immunology 2023Quote: ... and reverse transcribed (PrimeScript RT reagent kit, TaKaRa). Real-time qPCR was performed with the QuantStudio™ 6 Pro Real-Time PCR System (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... covering SIV env and nef were amplified from plasma viral RNA by nested RT-PCR using Prime-Script one-step RT-PCR kit v2 (TaKaRa) and KOD-Plus v2 (Toyobo) ...