Labshake search
Citations for Takara Bio :
4451 - 4500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... N-terminally and subcloned into pMSCV-IRES-Thy 1.1 vector (Clontech) using EcoRI and ClaI restriction sites.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... trizol reagent (Takara Bio, Shiga, Japan); PrimeScript 1st strand cDNA Synthesis Kit (Takara Bio ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... PrimeScript RT-PCR Kit (Takara Bio, Shiga, Japan)
-
bioRxiv - Synthetic Biology 2023Quote: ... and PrimeSTAR® GXL DNA Polymerase (TaKaRa) were used for PCR reactions to produce the DNA parts for genome modifications ...
-
bioRxiv - Plant Biology 2023Quote: ... RT-PCR was performed using SapphireAmp® Fast PCR Master Mix (Takara; RR350A), while iTaq™ Universal SYBR® Green Supermix (Biorad ...
-
bioRxiv - Plant Biology 2023Quote: ... The RNA was treated with gDNA Eraser (RR047A, Takara, Japan) to remove DNA contamination ...
-
bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR was performed using TB Green Premix Ex TaqTM (RR820A, Takara, Japan) with above cDNA as template ...
-
bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from three-week-old plants with Trizol reagent (9109, Takara, Japan). The RNA was treated with gDNA Eraser (RR047A ...
-
bioRxiv - Developmental Biology 2023Quote: ... coli (Takara Bio, 636763) via ampicillin-resistant selection ...
-
bioRxiv - Genetics 2023Quote: ... cDNA was amplified and purified using an Advantage 2 PCR Kit (Clontech). The cDNA library was sequenced using an Illumina sequencing platform (NovaSeq6000) ...
-
bioRxiv - Neuroscience 2023Quote: ... and insertion into the EcoRI and XbaI restriction sites of pZac2.1-GfaABC1D-Lck-GCaMP6f using In-Fusion cloning (TaKaRa). pHJ421(pEGFP-Ezrin WT ...
-
bioRxiv - Cell Biology 2023Quote: ... Greenberg) were cultured in DMEM supplemented with 10% tetracycline-free fetal bovine serum (Takara) and antibiotics ...
-
bioRxiv - Cell Biology 2023Quote: ... The primers for Sfrp1 In-fusion cloning (Takara Bio) are (Forward 5’-CTAGTGATT TCGCCGCCACCATGGGCGTCGGGCGCAGCGCGCG-3’ ...
-
bioRxiv - Cell Biology 2023Quote: ... The viral supernatants were collected 72 hr after transfection followed by virus concentration using Lenti-X concentrator (TaKaRa). Virus containing media was added to ATG3 KO HeLa cells with 8 μg/mL polybrene (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... Total RNA was reverse-transcribed with the PrimeScript RT kit (Takara, Kusatsu, Japan) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from 200 ng of total RNA by Prime script RT master Mix (Takara, RR036A). RT-qPCR was performed using TB Green Premix Ex TaqII (Tli RnaseH Plus ...
-
bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed using TB Green Premix Ex TaqII (Tli RnaseH Plus) (Takara, RR820L) and a Quantstudio6 Flex Real-Time PCR system (Thermo Fisher ...
-
bioRxiv - Neuroscience 2023Quote: ... by dispensing individual cells in 5 nL drops directly into 3 µL ice-cold single cell lysis buffer (scLB, 0.134% Triton X-100 [Sigma], 0.5 U/µL recombinant RNase inhibitor [Takara, 2313B] ...
-
bioRxiv - Bioengineering 2023Quote: ... murine T cells were transferred to retronectin-coated (Takara) plates previously spinoculated with retroviral particles at 2000ξg for 1.5h ...
-
bioRxiv - Developmental Biology 2023Quote: 2i was comprised of basal differentiation medium NDiff®227 (Takara), hereby referred to as N2B27 ...
-
bioRxiv - Cell Biology 2023Quote: ... using a Takara Thermal PCR Cycler (Takara Bio). RT-PCR of miRNAs was performed with a Taqman microRNA Reverse Transcription Kit (Invitrogen ...
-
bioRxiv - Cancer Biology 2023Quote: ... which was performed for genes of interest by using the SYBR Green Premix ExTaq (Tli RNaseH Plus) (Takara bio Inc, Japan) and the CFX96 Real-time PCR (Biorad ...
-
bioRxiv - Bioengineering 2023Quote: ... the lentivirus was concentrated using Lenti-X™ Concentrator (Takara, 631232) followed by transduction as detailed in the Isolation ...
-
bioRxiv - Immunology 2023Quote: ... and the cDNA was synthesized using the PrimeScript RT master kit (Takara, RR036A). The qPCR was done using PowerUp SYBR Green Master Mix (Thermofisher ...
-
bioRxiv - Genomics 2023Quote: ... a preliminary Terra PCR Direct Polymerase mix amplification (Takara Bio, Kusatsu, Japan) using 1µL of crude sample was used for direct genotyping without DNA purification ...
-
bioRxiv - Plant Biology 2023Quote: ... were converted to cDNA with oligo dT primers according to the instructions of the PrimeScript RT reagent Kit with gDNA Eraser (Takara, Japan). Quantitative PCR was performed in accordance with manufacturer’s instructions for THUNDERBIRD SYBR qPCR Mix (TOYOBO ...
-
bioRxiv - Neuroscience 2023Quote: ... 0.5 U*μl-1 RNAse inhibitor (Takara, 2313A). CsCl internal solution contains (in mM) ...
-
bioRxiv - Immunology 2023Quote: ... and cDNA was reverse-transcribed with MMLV reverse transcriptase (Clontech). Quantitative reverse-transcriptase polymerase chain reaction (qPCR ...
-
bioRxiv - Plant Biology 2023Quote: ... The resulting PCR products were assembled using Infusion cloning (Takara) to create the pMal-Strep-WEEP.
-
bioRxiv - Microbiology 2023Quote: ... and 5 µl of the treated mix were used to transform competent bacteria (Takara, Stellar™ Competent Cells ...
-
bioRxiv - Microbiology 2023Quote: Plasmids pGADT7 and pETDuet-1 were purchased from Clontech and Novagen respectively ...
-
bioRxiv - Microbiology 2023Quote: ... First-strand complementary DNA (cDNA) was synthesized with PrimeScript II Reverse Transcriptase (Takara) primed with a gene-specific oligonucleotide ...
-
bioRxiv - Microbiology 2023Quote: Lenti-X 293T cells (Clontech) and its derivative ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was made by transfecting Lenti-X 293T cells (Clontech 632180) with CTL plasmids plus psPAX2 and VSV-G packaging plasmids using Lipofectamine 2000 (Invitrogen 11668019) ...
-
bioRxiv - Immunology 2023Quote: The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation ...
-
bioRxiv - Immunology 2023Quote: ... the virus was concentrated using RetroX concentrator reagent (Takara). Meanwhile ...
-
bioRxiv - Immunology 2023Quote: ... RNA was extracted and libraries prepared using SMARTer Stranded RNA-Seq Kits according the manufacturer’s protocols (Takara) by the Integrated Genomics Operation (IGO ...
-
bioRxiv - Immunology 2023Quote: ... the viral supernatant was collected and Lenti-X™ GoStix™ Plus (Takara) was used to test presence of lentiviral p24 ...
-
bioRxiv - Genomics 2023Quote: ... coli (Takara Bioscience). 1% of the transformed cells were plated on ampicillin-agar plates to ensure adequate transformation efficiency (at least 10-fold library coverage) ...
-
bioRxiv - Genomics 2023Quote: ... Xfect (Takara Bioscience) transfection reagent was used ...
-
bioRxiv - Developmental Biology 2023Quote: All in-situ hybridization probe templates were amplified using Primestar-GXL (Takara) from cDNA prepared with SSIII (ThermoFisher ...
-
bioRxiv - Microbiology 2023Quote: ... The NucleoBond Xtra BAC kit was purchased from TaKaRa Bio (Kusatsu ...
-
bioRxiv - Immunology 2023Quote: ... and ChiPSC22 (Takara Bio Europe) were used ...
-
bioRxiv - Systems Biology 2023Quote: Molecular cloning was done by standard restriction enzyme digestion cloning, Gibson assembly (Gibson et al, 2009) or In-Fusion® cloning (Irwin et al, 2012) (TaKaRa Bio Inc., Kusatsu, Shiga, Japan). S ...
-
bioRxiv - Plant Biology 2023Quote: ... truncatula A17 using the primers MtU6.6_prom_InF_F1 and MtU6.6_prom_InF_R along with the guide cassette fragment amplified from pKSE401_RR using the primers pKSE401_empty_InF_F2 and pKSE401_InF_R2 (Table S1) and the amplified fragments were assembled into the HindIII digested pKSE401_RR vector backbone using In-Fusion cloning (Takara Bio, Kusatsu, Japan).
-
bioRxiv - Plant Biology 2023Quote: ... Promoter fragments were cloned into the pABAi vector (Takara Bio), and the pABAi plasmids carrying the promoter fragments were linearized and transformed into the Y1H Gold strain according to the Matchmaker protocol ...
-
bioRxiv - Plant Biology 2023Quote: ... Bait (pDEST-GBKT7) or prey (pDEST-GADT7) vectors were transformed into Saccharomyces cerevisiae strains AH109 Gold and Y187 (Clontech, MATCHMAKER GAL4 Two-Hybrid System) respectively using a classical heat shock protocol (Gietz and Woods ...
-
bioRxiv - Molecular Biology 2023Quote: ... Total protein was lysed by homogenization in radioimmunoprecipitation (RIPA) buffer (FUJIFILM) containing protease inhibitor cocktail (NACALAI TESQUE) and Cryonase Cold-active Nuclease (TAKARA), while total RNA was extracted using an RNeasy Mini Kit (Qiagen) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and CDS of fluorescent proteins were subcloned into pET-human αSyn51 using the KOD-Plus Mutagenesis kit (TOYOBO) and In-Fusion HD Cloning Kit (Takara Bio) according to the manufacturers’ protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... and proteinase K (9034, Takara Bio). The reactions were terminated by addition of 15% trichloroacetic acid ...