Labshake search
Citations for Takara Bio :
4101 - 4150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... prior to cells being fully resuspended to a single-cell suspension in 2mL NDiff227 with a 1mL pipette (Takara). Using a multichannel pipette ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μg/ml Doxycycline (Dox) (Clontech) was added into PGC-cultured FAcs medium 24 hours before the injection ...
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bioRxiv - Developmental Biology 2023Quote: ... and fluorescence quantitative kit (Takara Bio, Japan). The primer sequence is the same as Table 1 mentioned in 2.7
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR detection was performed on each group of samples by using a reverse transcription kit (Takara Bio, Japan) and fluorescence quantitative kit (Takara Bio ...
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bioRxiv - Developmental Biology 2023Quote: ... Polyadenylated RNA-seq libraries were prepared by using SMART-Seq v4 PLUS Kit (Clontech, Mountain View, CA, USA). Indexed RNA-seq libraries were sequenced using Illumina HiSeqX sequencer (paired end ...
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bioRxiv - Developmental Biology 2023Quote: ... lentivirus was first packaged following protocols we described previously1 and was then concentrated 100x using Lenti-X Concentrator kit (Takara, # 631231). Concentrated lentivirus was aliquoted and stored in -80°C before use ...
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bioRxiv - Developmental Biology 2023Quote: ... the PCR products were subcloned into EcoRV site of pBluescript SK- vector using In-Fusion® HD Cloning Kit (TaKaRa Bio, Japan) and sequenced with the M13 forward primer (5’-GTAAAACGACGGCCAG-3’ ...
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bioRxiv - Developmental Biology 2023Quote: ... at 1.6×106 cells/dish in RHB-A (Takara-Clontech), changing media every 24 h ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Inducible expression of a Nef-eGFP fusion protein was established in CEM-T4 cells using the Lenti-XTM Tet-On System (Clontech now Takara Bio USA) which places expression of Nef-eGFP under the control of a doxycycline-inducible promoter ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Spec.100) and TB Green TM Premix Ex TaqTM II (Tli RNaseH Plus) (RR820A, Spec. 200) were supplied by Takara (Beijing, China). IP cell lysate (P0013 ...
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bioRxiv - Plant Biology 2023Quote: ... and the RPS6A and RPS6B transcripts were amplified via PCR using PrimeStar Max polymerase (Takara). The PCR products were separated on a 1% agarose gel ...
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bioRxiv - Plant Biology 2023Quote: ... and inserted into the Aor51HI-linearized pMpGWB123/223/323/423 vectors using In-Fusion HD Cloning Kit (Takara Bio, Shiga, Japan).
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bioRxiv - Plant Biology 2023Quote: Coding sequences were cloned in the GAL4 system Gateway® vectors (pGADT7 and pGBKT7; Clontech) using primers listed in Dataset S1 ...
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bioRxiv - Plant Biology 2023Quote: ... Each sample of total RNA (1 mg) was reverse transcribed by the PrimeScriptTM RT reagent Kit with gDNA Eraser (cat no. RR047A, Takara, Japan). The resistance genes examined were the same as those investigated in our previous study 9.
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bioRxiv - Plant Biology 2023Quote: ... was PCR amplified from the pET-28a plasmid bearing WT or individual six-point mutations (M1 to M6) using HS Primestar DNA polymerase (Takara) with forward and reverse primers bearing BamHI and XhoI ...
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bioRxiv - Plant Biology 2023Quote: ... The full-length CAMTA3 cDNA was PCR amplified using the HS Primestar DNA polymerase (Takara, USA) with forward and reverse primers bearing BamHI and XhoI ...
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bioRxiv - Plant Biology 2023Quote: ... and RNA was reverse transcribed into cDNA with the TransScript Kit (TaKaRa, Dalian, China). RTLqPCR was performed with a StepOne Plus Real Time PCR System by using a TaKaRa SYBR Premix Ex Taq II reagent kit ...
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bioRxiv - Plant Biology 2023Quote: ... the chimeric H5Indo gene was reamplified by PCR and then introduced in the T-DNA region of a pCAMBIA binary vector previously linearized with restriction enzymes SacII and StuI using the In-Fusion cloning system (Clontech). Expression of H5Indo was driven by a 2X35S promoter from the cauliflower mosaic virus (CaMV) ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was isolated using RNAiso Plus (TaKaRa, Cat. #9109) and reverse transcribed to produce cDNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR was performed by using the TB Green Premix Ex Taq (Tli RNase H Plus; TaKaRa, Cat. #RR420A) and CFX Connect Real-Time system (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Bcl-2 and PTEN were detected by RT-PCR with TB Green TM Premix Ex TaqTM II(Tli RNaseH Plus) (RR820A, Takara, Japan). The complete gene sequences showed in Table 2 were searched from the National Center for Biotechnology Information (NCBI ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the KOD Xtreme Hot Start DNA polymerase kit (Takara) was utilized ...
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bioRxiv - Plant Biology 2023Quote: ... High fidelity Taq polymerase (PrimeSTAR GXL DNA polymerase, Takara) was used in the preparation of the PCR reaction mixture followed by the PCR program according to Gayatri et al ...
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bioRxiv - Synthetic Biology 2023Quote: Standard PCR reactions were performed using the PrimeSTAR Max DNA Polymerase (TaKaRa) or KOD One PCR Master Mix -Blue-(Toyobo) ...
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bioRxiv - Cell Biology 2023Quote: ... 500nM AP21967 (Takara) was added to the media at least 30 minutes prior to imaging.
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bioRxiv - Cell Biology 2023Quote: ... with 10% foetal bovine serum (tetracycline-free FBS, Takara Bio) and 1.2 μg ml−1 puromycin (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... with 5% foetal bovine serum (tetracycline-free FBS, Takara Bio) and 10 U mL−1 penicillin and 10 μg mL−1 streptomycin (Pen-Strep ...
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bioRxiv - Cancer Biology 2023Quote: ... iCaspase-T2A-tdTomato sequence was removed and swapped for mNeonGreen-NLS using a combination of Q5® High-Fidelity DNA Polymerase and the In-Fusion HD Cloning kit (Takara). Final targeting vector was generated by insertion of homology arms (∼600bp each ...
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bioRxiv - Cell Biology 2023Quote: ... and enzymatic digestion and ligation (TaKaRa, Japan) systems ...
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bioRxiv - Cell Biology 2023Quote: ... suspensions of the transformants were spotted onto SD plates with dropout supplements: Leu/-Trp (cat #630417) or -Ade/-His/-Leu/-Trp (cat #630428) (Takara Bio). The strains used in this assay are listed in Table S1.
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bioRxiv - Immunology 2023Quote: ... resuspended in CELLBANKER 1 (Takara), and stored at -80°C ...
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bioRxiv - Microbiology 2023Quote: ... and the PrimeScript cDNA synthesis kit (TaKaRa, Shiga, Japan) with 400 ng RNA input in a 10 μl reaction ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant AAV9 vectors were generated by co-transfection of AAVpro 293T cells (Takara Bio) with three plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... was inserted in between the KpnI and HindIII sites of the pOPINK vector (42) using the In-Fusion HD Cloning Kit (Takara). The pOPINK vector incorporates an N-terminal GST tag and a 3C protease cleavage site into the PhPINK1 construct ...
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bioRxiv - Molecular Biology 2023Quote: ... His6 (631212, Clontech, mouse, 1:500); NPL4 (sc-365796 ...
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bioRxiv - Microbiology 2023Quote: HEp-2 cells were transduced with supernatants of Plat-GP cells cotransfected with pMDG60 and pRetroX-Tet3G (TaKaRa), selected with 4 mg/ml G418 (Wako) ...
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bioRxiv - Microbiology 2023Quote: ... pAAV-UGI was constructed by cloning the entire coding sequence of UGI amplified from pBS-UGI-flag by PCR using the primers shown in S-Table 3 into the EcoRI and BamHI sites of pAAV-ZsGreen1 (TaKaRa).
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bioRxiv - Microbiology 2023Quote: ... pcDNA-EGFP-P2A-stop was constructed by amplifying the EGFP and P2A sequences from pEGFP-C1 (Clontech) using the primers shown in S-Table 3 and cloning it into the BamHI and EcoRI sites of pcDNA3.1(+ ...
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bioRxiv - Microbiology 2023Quote: ... and reverse transcribed using the TaKaRa PrimeScript RNA Kit (cat# RR036A, Takara, Japan), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... coli competent cells were used (Takara, https://www.takarabio.com/). Finally ...
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bioRxiv - Plant Biology 2023Quote: ... pDESTTM32RAPTOR1BcDNAwas co-transformed either with pDESTTM22FLZ14cDNA or pDESTTM22S6K1cDNA plasmids into the yeast strain Y2HGold (Takara Bio, USA) as previously described (Paiano et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... was performed by quantitative real-time polymerase chain reaction (PCR) using the AAVpro™ Titration Kit (for Real Time PCR) Ver.2 (Takara Bio Inc), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then incubated with anti-GFP antibody (JL-8; Clontech) at a dilution of 1:5,000 ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently stained using a horseradish peroxidase (HRP)-conjugated monoclonal anti-StrepTag antibody (Iba) or mouse anti-6His (Clontech) monoclonal antibody combined with rabbit anti-mouse-HRP polyclonal antibodies (DAKO) ...
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bioRxiv - Plant Biology 2023Quote: ... following treatment with RNAase-free DNAase (Takara). RNA (1.5 μg ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized using a cDNA synthesis kit (TaKaRa, Dalian, China), and the sequencing adapter was linked to both ends ...
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bioRxiv - Plant Biology 2023Quote: ... by using In-Fusion HD Cloning Kit (Takara Bio). Amino acid substitutions were introduced into pMAL-c5X Raf13 by site-directed mutagenesis to produce the kinase-dead Raf13 (K546N) ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR products were cloned into pMD19-T vectors (TaKaRa, Japan). Different UGT amino acid sequences were downloaded from NCBI ...
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed TB Green Premix Ex Taq II (Takara) and the StepOnePlus Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Lenti-X-293T cells were purchased from Clontech Laboratories ...