Labshake search
Citations for Takara Bio :
4051 - 4100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Quantitative RT-PCR was performed with a SYBR Premix Ex Taq II (Tli RNaseH Plus) (RR820; Takara) on a 7500 Real-Time PCR system (Thermo Fisher) ...
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bioRxiv - Genomics 2023Quote: ... using SMARTer® Stranded RNA-Seq Kit (Takara Bio USA, Inc.) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... coli StellarTM Competent Cells (Takara) according to manufacturer’s instructions and verified by sequencing ...
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bioRxiv - Genomics 2023Quote: ... The PCR reaction mix (TaKaRa LA Taq, catalog num. RR002M, Takara) was assembled using 24 μl of nuclease-free water ...
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bioRxiv - Genomics 2023Quote: ... The final 65 μl volume was treated with 5 μl of an enzymatic mixture containing: 0.1 μl of RNase H (60 U/μl, Ribonuclease H
, catalog num. 2150, Takara), 2 μl of RNase ONE (10 U/μl ... -
bioRxiv - Genomics 2023Quote: ... The PCR reaction mix (TaKaRa LA Taq, catalog num. RR002M, Takara) was assembled using 24 μl of nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: ... Remaining DNA was removed using the TURBO DNA-free kit (Thermo-Scientific) and cDNA generated using the PrimeScript cDNA synthesis kit (Takara). qRT-PCR reactions were set up according to the manufacturer’s instructions (Alkali Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... ISG20 expression was routinely induced upon incubation of cells for 18 hours with 1.5 μg/mL of doxycycline (Takara Bio, cat. 631311).
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bioRxiv - Neuroscience 2023Quote: Transgene constructs that simultaneously encode FingR targeting PSD95 and membrane markers of neuronal morphology were made using the In-Fusion HD Cloning System (Clontech). First ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized from 10 ng of total RNA using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: All plasmids were constructed using ligation-independent cloning (In-Fusion, Clontech) into the backbone of pAAV CaMKII iRFP-FLAG (Addgene 149513 ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293/17 cell media were harvested and concentrated overnight with a Lenti-X concentrator (Clontech, Mountain View, CA) according to the manufacturer’s protocol and resuspended in 10-fold less media volume prior to concentration.
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bioRxiv - Microbiology 2023Quote: ... A parallel virus production was obtained by substitution of the ISG20 coding viral genome with pRetroX-Tet-On (Clontech, cat. 632104) that codes for the TetOn protein and allows for dox ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed using the Thermal Cycler Dice Real Time System (Takara Bio, Kusatsu, Japan). Primers and templates were mixed with TB Green Premix Ex Taq II (Takara Bio) ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Clontech) and 2 mM L-glutamine (PAN Biotech ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was incubated with 4 ml TALON metal affinity resin (Clontech) at 4°C for 1 hr ...
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bioRxiv - Neuroscience 2023Quote: ... DNA from two selected colonies was amplified using LA Taq DNA polymerase (TaKaRa) and the region between the homology arms was sequenced ...
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bioRxiv - Microbiology 2023Quote: cDNA synthesis was performed using the PrimeScript RT kit (#RR037A, Takara Bio) using 250 ng RNA input from siRNA transfected HeLa MX2 cells ...
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bioRxiv - Microbiology 2023Quote: ... as previously described (17): pcDNA3.1+-based plasmids used for ectopic expression and pRetroX-tight-Puro-based ones (Clontech, cat. PT3960-5) used here to generate stable cell lines expressing ISG20 upon induction with doxycycline (dox.) ...
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bioRxiv - Microbiology 2023Quote: ... GFP tag (JL-8 monoclonal antibody from Takara), and mCherry (polyclonal antibody from Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... and quantitative PCR was performed using SYBR® Premix Ex Taq (Tli RNaseH Plus) (Takara) on a LightCycler 480 II (Roche ...
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bioRxiv - Microbiology 2023Quote: ... fus3::nyfp and cyfp::gal83 sequences were constructed on the pMD18-T (D101A, Takara, Dalian, China) plasmid with ClonExpress II One Step Cloning Kit (C112-01 ...
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bioRxiv - Microbiology 2023Quote: ... coli-Streptococcus shuttle vector pDL278 by using an In-Fusion HD cloning kit (TaKaRa, Japan) as described previously [49] ...
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bioRxiv - Microbiology 2023Quote: ... ssRNA was converted into cDNA using SMARTer Universal Low Input RNA Kit according to the manufacturer’s protocol (Takara Bio). After PCR amplification ...
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bioRxiv - Microbiology 2023Quote: ... and then treated with appropriate amounts of proteinase inhibitors (Takara Bio) and DNase I (Takara Bio ...
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bioRxiv - Immunology 2023Quote: ... using the Lenti-X concentrator (Takara Bio). Stable expression of soluble CD40L constructs was generated by lentiviral transduction of HEK293T/17 cells expressing BirA enzyme for in vivo biotinylation ...
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bioRxiv - Microbiology 2023Quote: ... LDH release was measured using an LDH Cytotoxicity Detection Kit (Clontech) according to the manufacturer’s instructions and normalized to mock-infected cells.
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bioRxiv - Microbiology 2023Quote: ... All the primers were designed using an online tool provided by TaKaRa (https://www.takarabio.com/learning-centers/cloning/primer-design-and-other-tools ...
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bioRxiv - Microbiology 2023Quote: ... FluPol Zhejiang-H7N9 was then purified using His60 NiNTA Superflow resin (Takara Bio) from the soluble fraction ...
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bioRxiv - Microbiology 2023Quote: ... we performed mutagenesis by overlapping PCR using PrimeSTAR GXL DNA Polymerase (TaKaRa, Cat# R050A) together with the primers IFNAR-F (5′-CGTGGGCCCGCGGCCGCGCCACCATG-3’ ...
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bioRxiv - Microbiology 2023Quote: ... and pMD2.G plasmids using TransIT-293 Transfection Reagent (TaKaRa, Cat# V2700) in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 1.25 units of ExTaq DNA polymerase (TAKARA, Shiga, Japan), 5 mM KCl ...
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bioRxiv - Immunology 2023Quote: ... to alanine (A) (L234A, L235A [LALA]) by site-directed mutagenesis with the In-Fusion Cloning System (In-Fusion® HD Cloning Plus; Clontech Laboratories Inc., Shiga, Japan) based on the manufacturer’s protocol [13].
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bioRxiv - Microbiology 2023Quote: ... cerevisiae strain PJ69-4A (Clontech, USA), which contains the lacZ gene from E ...
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bioRxiv - Microbiology 2023Quote: ... which is inducible by doxycycline (Takara Bio). For minigenome assays ...
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bioRxiv - Microbiology 2023Quote: ... pCMV-HA-N2 (Takara Bio Inc.,), pET28a(+ ...
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bioRxiv - Microbiology 2023Quote: ... and double amino acid mutations were introduced using PrimerSTAR® Max DNA Polymerase (# R046A, Takara Bio Inc.). The primers used for the mutation generation were listed in SI data.
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bioRxiv - Microbiology 2023Quote: ... The p24 amount in the medium was quantified by Lenti-X p24 Rapid Titer Kit (632200, Takara Bio Inc.) with the p24 control samples ...
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bioRxiv - Microbiology 2023Quote: The cDNA encoding human ZCCHC3 (NM_033089.7) was amplified from cDNA pool of HeLa cells by PCR and cloned into pEGFP-C3 (Takara Bio Inc., Shiga, Japan), pCMV-HA-N2 (Takara Bio Inc.,) ...
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bioRxiv - Microbiology 2023Quote: ... USA) or non-targeting control siRNA with TransIT-X2 Dynamic Delivery System (V6100, Takara Bio Inc.) in Opti-MEM ...
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bioRxiv - Microbiology 2023Quote: ... The cells were cultured again overnight and subjected to the quantification of mRNA by qRT-PCR using the CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (3732, Takara Bio Inc.), One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time ...
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bioRxiv - Microbiology 2023Quote: ... and small intestinal organoids by Total RNA extraction reagent RNAiso Plus (TaKaRa Bio, Shiga, Japan) or NucleoSpin®RNA (MACHEREY-NAGEL ...
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bioRxiv - Microbiology 2023Quote: ... The resulting PCR fragment was subcloned into the KpnI-NotI site of the pCAGGS vector20 using In-Fusion HD Cloning Kit (Takara, Cat# Z9650N). Nucleotide sequences were determined by DNA sequencing services (Eurofins) ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial DNA was isolated using a NucleoSpin DNA stool kit (Takara Bio, Shiga, Japan). The V3-V4 regions of the 16S rRNA gene were amplified in each sample ...
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bioRxiv - Microbiology 2023Quote: The Lenti-X 293T cell line (Takara, Cat# 632180) and HOS-ACE2/TMPRSS2 cells (kindly provided by Dr ...
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bioRxiv - Immunology 2023Quote: ... The RNA samples were dissolved in RNase-free water (TAKARA HOLDINGS Inc., Kyoto, Japan, 9012) and measured with a spectrophotometer (NanoDrop ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed in Thermal Cycler Dice Real Time System Lite (Takara Bio) using KOD SYBR qPCR Mix (Toyobo ...
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bioRxiv - Cell Biology 2023Quote: ... by replacing EGFP-coding sequences with mCherry-coding sequences using In-Fusion® HD Cloning Kit (Clontech).
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bioRxiv - Cell Biology 2023Quote: ... harvested in Trizol (Takara), and stored at -80 °C ...
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bioRxiv - Physiology 2023Quote: ... DNA was amplified using Terra PCR Direct Red Dye Premix (Takara Cat. No. 639286) and ran on a 1% agarose gel to confirm DNA recombination ...