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Citations for Takara Bio :
4001 - 4050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and the expression of specific gene was analysed using TB Green® Premix Ex Taq™ II (TaKaRa Bio Inc., Japan) in a Roche Light Cycler 480 with predesigned and validated primers from PrimerBank of Massachusetts General Hospital (61) ...
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bioRxiv - Neuroscience 2023Quote: ... and rabbit anti-dsRed2 (1:1000, Clontech). The following secondary antibodies were used ...
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bioRxiv - Neuroscience 2023Quote: PCR was performed using 1.25 U PrimeSTAR GXL DNA Polymerase (Takara, Japan) with 0.2 µM of forward and reverse primers ...
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bioRxiv - Neuroscience 2023Quote: ... Lentivirus was then concentrated using Lenti-X Concentrator (Takara Bio), reconstituted in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Each of the four pools was converted into one Illumina-barcode indexed sequencing library using the ThruPLEX DNA-Seq HV kit (Takara Bio Catalog# R400740), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: B/B homodimerizer was purchased from Takara USA lnc ...
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bioRxiv - Neuroscience 2023Quote: ... 5′-GCATGTCACGATGTTACAA-3′ and 5′-GGATGAAATGAAGGTGCTA-3′ as previously described50 and were inserted using the Infusion HD Cloning kit (Takara Bio USA Inc, NC1470242).
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bioRxiv - Neuroscience 2023Quote: ... and eGFP (Clontech. CA, USA) in tsA201 cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated in primary antibody (chicken anti-GFP, Abcam #13970, 1:2000, rabbit anti-DsRed, Takara Bio Clontech #632496, 1:200) for 24 – 48 hours in the dark while shaking at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... and the SYBR Green Real-time PCR Master Mix (Takara). Applied Biosystems™ 7500 Real-Time PCR Systems (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... pEGFP-N1 was obtained from Clontech-Takara.
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bioRxiv - Microbiology 2023Quote: ... Immunoblotting was performed using mouse anti-eGFP (Takara, 1:2000), rabbit anti-Actin (Bethyl ...
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bioRxiv - Microbiology 2023Quote: ... USA) and subcloned into the NheI and XhoI restriction sites of plasmid pIRES2-EGFP (Clontech, Takara Bio USA ...
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bioRxiv - Microbiology 2023Quote: ... USA) and subcloned into the NheI and XhoI restriction sites of plasmid pIRES2-EGFP (Clontech, Takara Bio USA, Mountain View, CA, USA). The coding sequences of the RBDs contain the secretion signal of the chicken receptor-type tyrosine phosphatase μ (RPTPμ ...
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bioRxiv - Molecular Biology 2023Quote: ... The same amount of total RNAs from each sample were reversely transcribed with PrimeScript RT Master Mix (TaKaRa, RR036A). Diluted cDNAs were mixed with primers and SsoAdvanced Universal SYBR® Green Supermix (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 × 106 PBMCs were plated on retronectin coated 6-well plates (Takara Clonetech) with retroviral vectors and centrifuged at 1,000 × g for 40 min ...
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bioRxiv - Microbiology 2023Quote: ... These PCR products were digested by SalI and NotI and subcloned in frame into pCMV2-HA (Clontech) cut by the same enzymes ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection was done using the CalPhos mammalian transfection kit (Clontech). Alternatively ...
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bioRxiv - Microbiology 2023Quote: ... We previously described the generation of psiRNA-U6GFP::Zeo and the nonsense negative control psiRNA-U6GFP::Zeo-shNS from the U6 RNA Polymerase III promoter in pSIREN-shuttle (Clontech) and psiRNA- 7SKGFP::Zeo (InvivoGen ...
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bioRxiv - Molecular Biology 2023Quote: ... and real-time PCR was performed using TB Green™ Premix Ex Taq™ (TaKaRa). For qRT-PCR ...
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bioRxiv - Microbiology 2023Quote: The pEGFP-C1 plasmids including AFF4 3’UTR sites “1-2-3” were generated using pEGFP-C1 (Clontech). The following complementary oligonucleotides were annealed in respective pairs as above (1.25 µM each in 75 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... 4μL of 20 mM DTT (TAKARA 639537), and 1.2μL of 100μM template-switch oligo [7] ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... The target area was initially amplified by PCR using PrimeSTAR® GXL polymerase (Takara). Amplicons were amplified again by PCR with TruSeq DNA-RNA CD index-containing primers to label each fragment with adapter and index sequences in order to build NGS libraries ...
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bioRxiv - Molecular Biology 2023Quote: RNA extracion from the cells and mouse hearts was performed using the sophisticated Trizol reagent (Takara, Kyoto, Japan). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA was subjected to reverse transcription to generate RNA cDNA using the widely recognized PrimeScriptRT kit (Takara, Kyoto, Japan) provided by the esteemed Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... using PCR and cloned in the pEGFP-N1 vector (Clontech) at the KpnI and NotI restriction sites ...
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bioRxiv - Molecular Biology 2023Quote: ... Accurate quantification of mRNA levels was meticulously conducted by employing the GAPDH reference gene and utilizing the cutting-edge SYBR Green kit (Takara, Kyoto, Japan) on the state-of-the-art Real-time PCR Detection System manufactured by Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... The amplified fragments and vector pBFv-UAS3 were fused using the In-Fusion® HD Cloning Kit (TaKaRa). The oligonucleotide sequences used are shown in Supplementary Table 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... and lentivirus was concentrated with lenti-X concentrator (Takara #631232) to 1/100th of the initial media volume and the lentivirus was resuspended in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... EGFP-SEC61B was generated by cloning the corresponding cDNA into the pEGFP.C1 vector (Clontech, 6084-1). The plasmid pRK5-HA-UB was described previously [56] ...
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bioRxiv - Microbiology 2023Quote: ... using an In-Fusion HD Cloning Kit (TaKaRa, Cat# Z9633N). The plasmid was amplified using NEB 5-alpha F′ Iq Competent E ...
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bioRxiv - Microbiology 2023Quote: Human kidney-derived Lenti-X 293T cells (TaKaRa, Cat# Z2180N) and porcine kidney-derived PK-15 cells (Japanese Collection of Research Bioresources Cell Bank ...
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bioRxiv - Microbiology 2023Quote: ... levels were measured by a qRT-PCR assay using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (TaKaRa, Cat# RR096A). The PCR protocol was 42°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... PK-15 cells were transfected with PX459-Ifnar1-k/o or PX459-Stat2-k/o plasmids using the TransIT-X2 Dynamic Delivery System (TaKaRa, Cat# V6100) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... We next cloned the synthesized DNA into the pDON-5 Neo-vector (TaKaRa, Kusatsu, Japan, Cat# 3657), which was pre-linearized with NotI-HF (New England Biolabs [NEB] ...
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bioRxiv - Microbiology 2023Quote: ... cloned into a pCold-II vector (Takara Bio Co Ltd., Japan) containing an N-terminal His tag ...
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bioRxiv - Molecular Biology 2023Quote: ... primer 0.2 μl (200 pmol/μl) and ExTaq HS 0.1 μl (TAKARA), and PCR analysis was performed under the following conditions ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-DsRed (TaKaRa Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... was reverse transcribed using PrimeScript™ RT Reagent Kit (Perfect Real Time) (Takara Bio, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... FD0564)-digested pCRIS-PITChv2-FBL plasmid (addgene #63672) using the In-Fusion HD cloning kit (Takara Bio, 639650). To create the sgRNA-plasmid backbone ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA (template) was obtained by using PrimeScript™ RT Reagent Kit (TaKaRa, Japan). The qRT-PCR reaction includes 10 μL SYBR qPCRMix (TOYOBO ...
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bioRxiv - Molecular Biology 2023Quote: The lentiviral backbone construct was designed starting from the commercial pLVX-EF1α-IRES-Puro vector (Takara Bio, 631253), to which a T2A-mTagBFP2 cassette (Twist Bioscience ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates was clarified by centrifugation as described above and 200 µl of TALON resin suspension (Takara Bio), equilibrated with the same buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The lentivirus-containing supernatant was concentrated around 40-fold using Lenti-X concentrator (Takara), aliquoted and stored in liquid nitrogen.
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bioRxiv - Molecular Biology 2023Quote: ... system (Takara). Flp-In-293 cells were maintained in DMEM with high glucose and L-glutamine supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten ng of cDNA were mixed with 1 x ExTaq buffer (TAKARA), 2.5 mM of each dNTP 2 μl ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified and ligated into the pMD-20 vector or the pANT vector using the Mighty TA- cloning Kit (Takara) or TA-Enhancer Cloning Kit (Nippon Gene ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from whole brains of mice using Trizol agent (Takara, Japan) and treated with DNase following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... mcu-1 sequence was amplified from worm cDNA using PrimeSTAR MAX DNA Polymerase (Takara) and cloned into pCR-Blunt II-TOPO vector (Invitrogen).
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated in primary antibody (chicken anti-GFP, Abcam #13970, 1:2000, rabbit anti-DsRed, Takara Bio Clontech #632496, 1:200) for 24 – 48 hours in the dark while shaking at 4 °C ...