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Citations for Takara Bio :
3951 - 4000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: Cloning of RAP2.4pro:RAP2.4–venusYFP was done according to standard In–fusion cloning procedures (Takara In–Fusion® Snap Assembly Master Mix #638948) ...
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bioRxiv - Physiology 2023Quote: ... DNA was amplified using Terra PCR Direct Red Dye Premix (Takara Cat. No. 639286) and ran on a 1% agarose gel to confirm DNA recombination ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Inducible expression of a Nef-eGFP fusion protein was established in CEM-T4 cells using the Lenti-XTM Tet-On System (Clontech now Takara Bio USA) which places expression of Nef-eGFP under the control of a doxycycline-inducible promoter ...
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bioRxiv - Physiology 2023Quote: ... Slides were incubated for 18 hours at room temperature in anti-dsRed antibody (TaKaRa, 1:2000) in 0.1M PB containing 0.3% Triton X-100 and 1% donkey serum ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was isolated using RNAiso Plus (TaKaRa, Cat. #9109) and reverse transcribed to produce cDNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR was performed by using the TB Green Premix Ex Taq (Tli RNase H Plus; TaKaRa, Cat. #RR420A) and CFX Connect Real-Time system (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... Thermal Cycler Dice Real Time System III (Takara), or 7500 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (ref.69) (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). On day 3 (24 hours post-transfection) ...
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bioRxiv - Neuroscience 2023Quote: ... mRNA was transcribed into full-length complementary DNA using a SMART-Seq v4 Ultra Low Input RNA kit (Clontech) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... coated with 1 µg/cm2 of iMatrix-511 (Takara Bio). Hypothalamic cultures were loaded with the Fluorescent Dye-Based Cal-590 following the manufacturers’ instructions (Stratech) ...
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bioRxiv - Neuroscience 2023Quote: Libraries were constructed with the SMARTer® Stranded Total RNA-Seq Kit – Pico Input Mammalian kit (Clontech Laboratories), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Promoterless bicistronic vectors were generated by removing the SV40 promoter from the bicistronic vector pRUF and the pRUF vectors containing the putative IRES sequences by PCR amplification using divergent primers (Supplementary Table 3) and the CloneAmp™ HiFi PCR Premix (Takara) polymerase ...
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bioRxiv - Molecular Biology 2023Quote: ... linearized by PCR and using the divergent primers reported in Supplementary Table 3 and CloneAmp™ HiFi PCR Premix (Takara) polymerase ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-dsRed (Takara Bio) 1:500 ...
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bioRxiv - Microbiology 2023Quote: ... The protocol was as follows: PCR reaction mixture (100 nL) was prepared using SmartChip TB Green Gene Expression Master Mix (Takara Bio, Shiga, Japan), nuclease-free PCR-grade water ...
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bioRxiv - Developmental Biology 2023Quote: ... The yeast two-hybrid library was generated from the poly(A)+ mRNA isolated from mixed-stage N2 nematodes using the Matchmaker kit (Clontech) as per the manufacturer’s protocol and transformed into Y1HGold strain (MATα ...
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bioRxiv - Developmental Biology 2023Quote: ... fbf-2 cDNA was cloned into the pGBTK vector (Clontech) and transformed into PJ68-4a yeast strain (MATa trp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ ...
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bioRxiv - Bioengineering 2023Quote: ... Lentivirus was then concentrated 100X using the Lenti-X concentrator (Takara Bio,631232). Biological titration of lentivirus by QPCR was carried out as previously described86 ...
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bioRxiv - Bioengineering 2023Quote: ... 37°C for 2 hours in a plate coated with Retronectin (Takara Bio, T100B) with an MOI of ∼5.0 for each lentivirus (dCas9 lentivirus at MOI ∼5.0 and gRNA-MCP-fusion effector lentivirus) ...
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bioRxiv - Cell Biology 2023Quote: ... The full-length was also cloned into the lentiviral vector pLVX-TetOne-Puro (Clontech, Takara, #631849). The plasmid pECMV-3×FLAG-RAB11A was purchased from SinoBiological (Beijing ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time qPCR amplification was performed using the PrimeScript™ RT-PCR Kit (Takara, Catalog#RR420). Alp ...
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bioRxiv - Cell Biology 2023Quote: ... Single-stranded cDNA was reverse transcribed from 500ng total RNA using reverse transcriptase and oligo(dT) primer (Takara, Catalog#RR036). Real-time qPCR amplification was performed using the PrimeScript™ RT-PCR Kit (Takara ...
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bioRxiv - Cancer Biology 2023Quote: ... Viruses were concentrated with lenti-X concentrator (Clontech). Cell lines to be infected with virus were seeded with 200,000 cells in each well of a 6-well plate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Bcl-2 and PTEN were detected by RT-PCR with TB Green TM Premix Ex TaqTM II(Tli RNaseH Plus) (RR820A, Takara, Japan). The complete gene sequences showed in Table 2 were searched from the National Center for Biotechnology Information (NCBI ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the KOD Xtreme Hot Start DNA polymerase kit (Takara) was utilized ...
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bioRxiv - Plant Biology 2023Quote: ... High fidelity Taq polymerase (PrimeSTAR GXL DNA polymerase, Takara) was used in the preparation of the PCR reaction mixture followed by the PCR program according to Gayatri et al ...
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bioRxiv - Plant Biology 2023Quote: ... was PCR amplified from the pET-28a plasmid bearing WT or individual six-point mutations (M1 to M6) using HS Primestar DNA polymerase (Takara) with forward and reverse primers bearing BamHI and XhoI ...
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bioRxiv - Plant Biology 2023Quote: ... The full-length CAMTA3 cDNA was PCR amplified using the HS Primestar DNA polymerase (Takara, USA) with forward and reverse primers bearing BamHI and XhoI ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Spec.100) and TB Green TM Premix Ex TaqTM II (Tli RNaseH Plus) (RR820A, Spec. 200) were supplied by Takara (Beijing, China). IP cell lysate (P0013 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HepG2 cells were resuspended in ice-cold PBS supplemented with protease inhibitors (Clontech, Mountain View, CA, USA) and lysed in ice bath by sonication in cycles of 10 s on /5 s off for 1 min at 6–10 μm amplitude at 25 % intensity from an exponential ultrasonic horn of 3 mm in a Soniprep 150 MSE (MSE Ltd. ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The PCR products were used as templates to synthesize specific dsRNA using the in vitro Transcription T7 Kit (TaKaRa Biotechnology, Dalian, China) (dsRNA-Vitro ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from tissues and cells was extracted using TRIzol reagent (Takara, Japan) and were reversed transcribed into first-strand cDNA using the PrimeScript RT Reagent Kit (Takara ...
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bioRxiv - Cell Biology 2023Quote: ... and were reversed transcribed into first-strand cDNA using the PrimeScript RT Reagent Kit (Takara) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... These plasmids were transformed into the yeast strain AH109 for testing protein-protein interaction using the Matchmaker Gold system (Takara Bio, Kusatsu, Japan). Yeast transformation and growth were performed as described in [Pecher et al. ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting GST-S-LacI fusion sequence was then amplified by PCR using primers eo1211 and eo1212 (Supplementary Table S1) and inserted between the NdeI and HindIII sites in the pCold II vector (Takara Bio, Shiga, Japan, #3362) by the In-Fusion reaction ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysate was then filtered through a 0.45 µm syringe filter and mixed with 2 mL of washed TALON® Metal Affinity Resin beads (Takara). The mixture was allowed to bind under nutation at 4℃ for 1 hr ...
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bioRxiv - Biochemistry 2023Quote: TssM193-490 coding region was obtained by gene synthesis (IDT) and cloned into pOPIN-K vector45 using the In-Fusion HD Cloning Kit (Takara Clontech). Point mutations were introduced using the QuikChange Lightning kit (Agilent Technologies).
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bioRxiv - Biochemistry 2023Quote: ... The amplified vector was gel purified (1% agarose) and used for In-Fusion® HD cloning (Clontech, Mountain View, CA, USA) with amplified and gel purified (2% agarose ...
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bioRxiv - Biochemistry 2023Quote: ... The endogenous pfrkip locus was targeted by cloning 20 nucleotide guide region (5’-ATAATTGGTGCCAAATTGAA-3’) with primer pair GRKIPV5F/ GRKIPV5R using In-Fusion (Clontech, Mountain View, CA) in pL6eGFP plasmid [53] generating pL6eGFPgV5 plasmid ...
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bioRxiv - Biochemistry 2023Quote: ... was generated by amplifying the coding sequence of human EL from cDNA (LIPG; MGC MHS6278-202806078) and inserting it into the vector pCDNA6 using InFusion cloning (Clontech). An EL containing a T111I mutation (pKS17 ...
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bioRxiv - Cancer Biology 2023Quote: ... Pico Input Mammalian (Takara Bio) according to manufactures instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted by using MiniBEST Universal RNA extraction Kit (TaKaRa) according to the manufacturer’s instructions and quantified by NanoDrop instruments (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNA regions were amplified using staggered primers (see below) by Ex Taq (TaKaRa, Catalog # RR001B) to construct a library for next-generation sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... bacteria cells were pelleted and BAC DNA was extracted using the NucleoBond Xtra BAC kit (Takara, 740436) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... followed by the reverse tTA (rtTA) gene (Tet-On 3G, Takara Bio), separated via self-cleaving T2A peptide was generated via Gibson Assembly (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... LNCaP KO and LNCaP NT control cells were transiently transfected with 150ng of NF-kB luciferase reporter gene (NF-kB-luc, Clontech) and 3.5ng of Renilla using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T Lenti-XTM cells (Takara Bio Europe ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing media was harvested for virus isolation using Lenti-X concentrator (Takara) in lieu of ultracentrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... Additional lentiviral vectors were packaged in HEK 293T cells using Lenti-X Packaging Single Shots (VSV-G) (Takara Bio USA) per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... pLVX-Tet-Off Advanced and pLVX Tight-Puro-GOI vectors were purchased from Takara Bio (cat#632163 ...