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Citations for Takara Bio :
3901 - 3950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The respective gene targets were amplified by polymerase chain reaction using Ex Taq polymerase (Takara Bio, catalog no. RR01CM) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... pETCH-VanRB and pGro7 (Takara Cat# 3340) were cotransformed into BL21(DE3 ...
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bioRxiv - Biochemistry 2023Quote: PCR reactions were performed with PrimeSTAR MAX DNA Polymerase (BD Clontech GmbH, Heidelberg, Germany) or Phusion High-Fidelity polymerase (Thermo Fisher Scientific GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... AP21967 (rapalog) from Takara, Doxycycline (Cas No.24390-14-5 ...
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bioRxiv - Biochemistry 2023Quote: ... All PCR was done with HiFi PCR master mix (Takara) following the manufacturer recommendations.
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bioRxiv - Biochemistry 2023Quote: ... followed by an SGG linker and then the ferritin protein1 were cloned into the pADD2 vector backbone using HiFi PCR (Takara) followed by In-Fusion (Takara ...
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bioRxiv - Bioengineering 2023Quote: ... Protease inhibitors and 10% glycerol were added to the supernatant before loading it onto 1 mL of Talon metal affinity resin (Clontech, Mountain View, CA, #635502). After a 20 mL buffer wash ...
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bioRxiv - Bioengineering 2023Quote: ... On Day 2 virus coated culture plates were prepared on non-TC-treated 12-well plates that had been pre-coated with RetroNectin (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was incubated with 500 µL of a TALON resin (Clontech) for 16 h.
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bioRxiv - Cancer Biology 2023Quote: ... Gamma-retroviral transduction was facilitated by RetroNectin (#T100B, Takara Bio). Transduced and non-transduced (NT ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription of mRNA was performed using 3-5 μg RNA with RNA to cDNA EcoDry Premix (Takara, #639549). For real-time PCR analysis ...
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bioRxiv - Bioengineering 2023Quote: ... PrimeStar HS DNA polymerase was purchased from Takara (Dalian, China). The RAA kit was purchased from Hangzhou ZhongCe BioSci&Tech Co ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from adult hippocampal NSCs using RNAiso Plus (9109, Takara Bio) and reverse transcribed using RevaTra Ace qPCR RT Master Mix with gDNA Remover (FSQ-301 ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were fixed with ice-cold 4 % paraformaldehyde for 20 min and stained with rabbit anti-dsRed antibody (Takara Bio) and Alexa fluor 568-conjugated anti-rabbit secondary antibody to identify cells expressing mApple-tagged Myo6 ...
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bioRxiv - Physiology 2023Quote: ... Lentivirus was collected beginning 48-hrs post-transfection and concentrated with Lenti-X Concentrator (Takara Bio USA, Mountain View, CA) for 3 consecutive days ...
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bioRxiv - Physiology 2023Quote: ... followed by the use of EcoDry RNA to cDNA (Takara Bio Inc. ...
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bioRxiv - Immunology 2023Quote: cDNA was amplified from the collected cells using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-TakaraBio). 1ng of amplified cDNA was used to generate barcoded libraries with the Nextera XT DNA library preparation kit (Illumina) ...
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bioRxiv - Genetics 2023Quote: ... the sequences corresponding to Flag tags were replaced by those encoding for Myc tags using the In-Fusion PCR cloning system (Takara) according to the kit’s guidelines ...
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bioRxiv - Genetics 2023Quote: ... according to the steps in the instruction book of SYBR PrimeScript RT-PCR kit (Takara). We used 2-ΔΔc to indicate the relative gene expression level of each sample.
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bioRxiv - Cell Biology 2023Quote: ... hygromycin B (300 µg/ml) and 10% tetracycline free fetal bovine serum (Takara Bio, USA) in a humidified incubator at 37°C in 5% CO2 in 6-well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... Adenoviruses were titered using the Adeno-X Rapid Titer Kit (TaKaRa).
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bioRxiv - Cancer Biology 2023Quote: ... the agarose gel pieces were cut out and digested using NucleoSpin® Gel and PCR Clean-Up kit (Takara, 740609), according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by insertion of a flexible linker72 at the C-terminus of EGFP using the InFusion system (Takara). α-tubulin CDS was amplified from a cDNA clone ...
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bioRxiv - Developmental Biology 2023Quote: ... They were then incubated O/N at 4°C with the corresponding primary antibody: anti-DsRed (1:500; Clontech), mouse anti-GFP ([1:100] ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was extracted using a NucleoSpin Tissue XS kit (TAKARA Bio USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ HSPCs were cultured overnight on RetroNectin-coated (Takara Bio USA) plates in StemSpan SFEM II medium supplemented with SCF 100 ng/mL ...
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bioRxiv - Genomics 2023Quote: ... except for gRNA cloning that was performed using In-Fusion cloning (Takara) and the drug-selection cassette of blasticidin instead of human dhfr was used ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription was carried out using SmartScribe RT (Takara), and Q5 polymerase (New England Biolabs ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR was performed with ExTaq (TaKaRa, Japan) on 2720 thermal cycler (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... Full length cDNAs were generated from 350 to 1,000 pg of total RNA using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe ...
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the OneStep TB Green PrimeScript PLUS RT-PCR kit (TAKARA, Cat# RR096A) and the following primers ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cells using RNAiso Plus Regent (Takara, Cat# 9108) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 14-3-3τ and β-actin were designed and synthesized by Takara (Table 1). To run the real-time PCR reaction ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit anti-Myc (1:1000) (Clontech); rabbit anti-HA (1:800 ...
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bioRxiv - Cell Biology 2023Quote: ... The real-time PCR reaction was performed using One Step SYBR Prime Script RT-PCR Kit (TakaRa). The primers for 14-3-3β ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells were sorted by FACS as described above into 96-well plates containing 0.1 μl of RNase inhibitor (Clontech, Cat# 2313A), 1.9 μl of 0.2% Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cells using RNAiso Plus Regent (Takara, Cat# 9108) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The cDNA is synthesized using Prime Script RT reagent Kit with gDNA Eraser (TakaRa). The RT reaction was carried out for one cycle at 42℃ for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... pBI-BFP (made by replacing AcGFP1 in pBI-CMV2 Vector (Clontech) with BFP from pTagBFP-N Vector (Evrogen ...
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bioRxiv - Plant Biology 2023Quote: ... and RNA was reverse transcribed into cDNA with the TransScript Kit (TaKaRa, Dalian, China). RTLqPCR was performed with a StepOne Plus Real Time PCR System by using a TaKaRa SYBR Premix Ex Taq II reagent kit ...
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bioRxiv - Plant Biology 2023Quote: ... the chimeric H5Indo gene was reamplified by PCR and then introduced in the T-DNA region of a pCAMBIA binary vector previously linearized with restriction enzymes SacII and StuI using the In-Fusion cloning system (Clontech). Expression of H5Indo was driven by a 2X35S promoter from the cauliflower mosaic virus (CaMV) ...
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bioRxiv - Plant Biology 2023Quote: ... and the RPS6A and RPS6B transcripts were amplified via PCR using PrimeStar Max polymerase (Takara). The PCR products were separated on a 1% agarose gel ...
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bioRxiv - Plant Biology 2023Quote: ... and inserted into the Aor51HI-linearized pMpGWB123/223/323/423 vectors using In-Fusion HD Cloning Kit (Takara Bio, Shiga, Japan).
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bioRxiv - Plant Biology 2023Quote: Coding sequences were cloned in the GAL4 system Gateway® vectors (pGADT7 and pGBKT7; Clontech) using primers listed in Dataset S1 ...
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bioRxiv - Plant Biology 2023Quote: ... Each sample of total RNA (1 mg) was reverse transcribed by the PrimeScriptTM RT reagent Kit with gDNA Eraser (cat no. RR047A, Takara, Japan). The resistance genes examined were the same as those investigated in our previous study 9.
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bioRxiv - Plant Biology 2023Quote: ... The yeast transformants were grown on nutrient-restricted (without Trp, Leu, His, Ade) mediums (Clontech) 3-5 days to assess interactions between various protein combinations.
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bioRxiv - Plant Biology 2023Quote: ... Reactions contained 0.5 µL of hot-start high fidelity Clonetech PrimeSTAR GLX polymerase (Takara Bio, Kusatusu, Japan), 5 µL of 5X buffer ...
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bioRxiv - Plant Biology 2023Quote: ... Reactions used 0.2 µL of hot-start high fidelity Clonetech PrimeSTAR GLX polymerase (Takara Bio, Kusatusu, Japan), 2 µL of 5X buffer ...
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bioRxiv - Plant Biology 2023Quote: The nucleotide sequence (around 500 bp long) specific to the NlCA coding region was cloned into the pMD 19-T vector (TAKARA). The double-stranded RNA was synthesized through PCR amplification by using the Mega script T7 High Yield RNA Transcription Kit (Vazyme ...
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bioRxiv - Synthetic Biology 2023Quote: Standard PCR reactions were performed using the PrimeSTAR Max DNA Polymerase (TaKaRa) or KOD One PCR Master Mix -Blue-(Toyobo) ...